scholarly journals Effects of Additives on Silage Fermentation Characteristic and In Vitro Digestibility of Perennial Oat at Different Maturity Stages on the Qinghai Tibetan

2021 ◽  
Vol 9 (11) ◽  
pp. 2403
Author(s):  
Ping Li ◽  
Xiaolong Tang ◽  
Chaosheng Liao ◽  
Maoya Li ◽  
Liangyin Chen ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Dry Matter ◽  
Gas Production ◽  
Coliform Bacteria ◽  
In Vitro Digestibility ◽  
Flowering Stage ◽  
Ambient Temperatures ◽  
Dry Matter Digestibility ◽  
Silage Fermentation

To effectively use local grass resources to cover the winter feed shortage on the Qinghai-Tibetan Plateau, the silage fermentation and in vitro digestibility of perennial oat (Helictotrichonvirescens Henr.) were investigated. Perennial oat was harvested at the heading/flowering stage, wilted under sunny conditions, chopped, vacuumed in small bag silos, and stored at ambient temperatures (5–15 °C) for 60 days. The silages were treated without (CK) or with local lactic acid bacteria (LAB) inoculant (IN1), commercial LAB inoculant (IN2), and sodium benzoate (BL). Control silages of perennial oat at early heading stage showed higher (p < 0.05) lactate and acetate contents and lower (p < 0.05) final pH, butyrate, and ammonia-N contents than those at the flowering stage. High levels of dry matter recovery (DMR) and crude protein (CP) were observed in IN1- and BL-treated silages, with high in vitro gas production and dry matter digestibility. Compared to CK, additives increased (p < 0.05) aerobic stability by inhibiting yeasts, aerobic bacteria, and coliform bacteria during ensiling. In particular, the local LAB inoculant increased (p < 0.05) concentrations of lactate, acetate and propionate, and decreased concentrations of butyrate and ammonia-N in silages. This study confirmed that local LAB inoculant could improve the silage quality of perennial oat, and this could be a potential winter feed for animals such as yaks on the Qinghai Tibetan Plateau.

Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Application Rates ◽  
The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

scholarly journals THE IN VITRO DIGESTIBILITY OF WHOLE GRASSES AND THEIR PARTS AT PROGRESSIVE STAGES OF MATURITY

1963 ◽  
Vol 43 (1) ◽  
pp. 79-87 ◽  
Author(s):  
G. I. Pritchard ◽  
L. P. Folkins ◽  
W. J. Pigden
Keyword(s):  
Tall Fescue ◽  
Dry Matter ◽  
Growing Season ◽  
The Other ◽  
Rapid Decline ◽  
In Vitro Digestibility ◽  
Flowering Stage ◽  
Dry Matter Digestibility ◽  
Rate Of Decline

The in vitro dry matter digestibility (IVD) of timothy, orchard, brome, reed canary, tall fescue and mountain rye grasses decreased at the rate of approximately 0.5 per cent per day throughout the growing season. The most rapid decline in IVD began with head emergence. The earliest maturing grasses tended to be higher in IVD than the other grasses at the flowering stage. The decline in IVD as the season progressed was observed in all portions of the plant, the rate of decline for the heads and stems being greater than for the leaves. The upper segments of the stems tended to have a lower IVD than the basal segments.

scholarly journals 134 Effects of Aspergillus oryzae prebiotic on in vitro digestibility and fermentability of fibrous ingredients and diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 110-110
Author(s):  
Jinlong Zhu ◽  
Jae-Cheol Jang ◽  
Gerald C Shurson ◽  
Lynsey Whitacre ◽  
Ignacio R Ipharraguerre ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Dry Matter ◽  
Mixed Model ◽  
Enzymatic Digestion ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Production Kinetics ◽  
In Vitro Hydrolysis

Abstract It has been an increasing interest to develop strategies to improve utilization of fibrous ingredients in swine. The objective of this study was to determine the effects of an Aspergillus oryzae prebiotic (AOP, Amaferm®) on in vitro digestibility and fermentability of energy and dry matter using a 3-step in vitro enzymatic digestion and fermentation. Three diets were formulated based on corn-DDGS (DDGS), rice bran (RB), and wheat middlings (WM). During in vitro hydrolysis and fermentation, 0.05% AOP was added to the diets (DDGS+AOP, RB+AOP, and WM+AOP) and sole ingredients. Each 2-g sample was hydrolyzed for 2 h with pepsin and for a subsequent 4 h with pancreatin. Hydrolyzed residues were filtered, washed, dried, weighed, pooled within the same sample, and used for subsequent fermentation using swine fecal inoculum. Volume of gas produced was recorded at 11 time points during 72 h of incubation. Parameters of gas production kinetics were calculated using a nonlinear monophasic model, and differences among treatment groups were compared using a mixed model. Supplementation of AOP increased (P &lt; 0.05) in vitro digestibility of gross energy (IVDGE) and dry matter (IVDDM) in the diets and AOP tended to increase (P = 0.06) dry matter fermentability of the diets. Supplementation of AOP during fermentation did not (P &gt; 0.1) affect maximum gas production in ingredients or diets. AOP decreased the half-time to asymptote (T/2) in the ingredient of DDGS+AOP compared with that of DDGS and increased the fraction rate of degradation at T/2 in the diet of RB+AOP compared with that of RB. In conclusion, supplementation of AOP increased energy and dry matter digestibility of the diets during in vitro hydrolysis and modified gas production kinetics during in vitro fermentation and the effects were dietary- and ingredient-type dependent.

scholarly journals COMPARISON OF NEAR INFRARED CALIBRATIONS FOR ESTIMATING IN VITRO DIGESTIBILITY IN WHOLE-PLANT CORN HYBRIDS

1987 ◽  
Vol 67 (2) ◽  
pp. 557-562 ◽  
Author(s):  
E. V. VALDES ◽  
R. B. HUNTER ◽  
G. E. JONES
Keyword(s):  
Dry Matter ◽  
Near Infrared ◽  
Mature Stage ◽  
In Vitro Digestibility ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
Corn Hybrids ◽  
Dry Matter Digestibility ◽  
Whole Plant

A comparison of two near infrared (NIRA) calibrations (C1 and C2) for the prediction of in vitro dry matter digestibility (IVDM) in whole-plant corn (WPC) was conducted. C1 consisted of 40 WPC samples collected from four locations across Ontario (Brucefield, London, Guelph and Elora). C2 consisted of 90 samples and included the above locations plus Pakenham and Winchester. Nine wavelengths were used in both equations but only three were common in C1 and C2 equations. These wavelengths were 2139 nm, 2100 nm, and 1445 nm, respectively. The predictions of IVDM utilizing both C1 and C2 were good. Coefficients of determination (r2) and standard error of the estimate (SEE) for calibration and prediction sets were 0.91, 1.7; 0.85, 1.7 for C1 and 0.88, 1.6; 0.77, 1.6 for C2 respectively. Regression analysis within location, however, showed low r2 values for the prediction of IVDM for Pakenham and Winchester in both calibrations. The more mature stage of harvest at these locations might be the cause of the poorer predictions. Key words: In vitro digestibility, whole-plant corn, near infrared reflectance

THE IN VITRO DIGESTIBILITY OF PLANT PARTS OF ORCHARDGRASS CLONES WITH ADVANCING STAGES OF MATURITY

1965 ◽  
Vol 45 (5) ◽  
pp. 503-507 ◽  
Author(s):  
D. N. Mowat ◽  
B. R. Christie ◽  
J. E. Winch
Keyword(s):  
Dry Matter ◽  
In Vitro Digestibility ◽  
Plant Parts ◽  
Dry Matter Digestibility ◽  
Total Leaf ◽  
Early Maturing

The in vitro dry matter digestibility (I.V.D.) of the stem and total leaf fractions of four orchardgrass clones was similar at the preheading stage. Even at 100% head emergence, differences in I.V.D. between stems and living leaves were not great with the early-maturing clones. However, wide differences existed between the values for stems and living leaves in mature plants. Heads were only slightly higher than stems in digestibility.Leaf digestibility was lower for those leaves dead or even partially dead. Nevertheless, in mature plants, dead leaves were higher in I.V.D. than heads or stems. Large differences occurred among clones in stem digestibility. However, the variability among clones in leaf digestibility was small.

scholarly journals Chemical composition and ruminal degradation kinetics of white oat (Avena sativa L.) IPR 126

2017 ◽  
Vol 18 (1) ◽  
pp. 50-61 ◽  
Author(s):  
Silvonei Tiago RICACHESKI ◽  
Douglas Sampaio HENRIQUE ◽  
Lilian Regina Rothe MAYER ◽  
Jhone Gleison de OLIVEIRA ◽  
Jucemara Aparecida ROSLER ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Dry Matter ◽  
Degradation Kinetics ◽  
Gas Production ◽  
Repeated Measurements ◽  
Chemical Components ◽  
In Vitro Digestibility ◽  
Estimation Of Parameters ◽  
Ruminal Degradation

SUMMARY The present study aiming to determine the nutritional quality of oat (Avena sativa L.) IPR 126 in order to produce forage for ruminants. Four periods between harvests were used: 14, 21, 28 and 35 days, distributed in randomized blocks with four replicates for each treatment. The variables evaluated were: the concentration of dry matter (DM), neutral detergent insoluble fiber (NDF), crude potein (CP), lignin, dry matter (DM), ash, ether extract (EE) and in vitro digestibility of dry matter obtained by measurement of gas production. The profile of gas production was adjusted to the logistic bicompartimental mathematical model. The variables and the parameters of the adjusted gas production curves were analyzed as repeated measurements through the PROC MIXED of SAS (version 9.0) and the restricted maximum likelihood (REML) as the method of estimation of parameters. Regression analysis was performed for the variables: DM, CP, EE, NDF, ash, and for the parameter k2 of the bicompartimental model. DM and NDF concentrations increased linearly, CP, ash, and the estimations of the parameter k2 reduced linearly and the EE concentration showed a cubic behavior in function of the age of harvest. Lignin and other parameters of the Schofield model were not influenced by the age of harvest. The harvest interval influenced some chemical components and degradation rate of fiber carbohydrates, but do not interfere in lignin concentration. The forage with 21 days of cutting interval has the high nutritional value.

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

scholarly journals Yield and nutritional value of BRS Capiaçu grass at different regrowth ages

2019 ◽  
Vol 40 (5) ◽  
pp. 2045 ◽  
Author(s):  
Flávio Pinto Monção ◽  
Marco Aurélio Moraes Soares Costa ◽  
João Paulo Sampaio Rigueria ◽  
Marielly Maria Almeida Moura ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  
Keyword(s):  
Dry Matter ◽  
Northern Region ◽  
Matter Content ◽  
Neutral Detergent Fiber ◽  
Pennisetum Purpureum ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Elephant Grass ◽  
Dry Matter Digestibility

The objective was to evaluate the productivity, chemical composition, ruminal degradability of dry matter and digestibility of BRS capiaçu grass (Pennisetum purpureum Schum.) managed at five regrowth ages. A completely randomized design with ten replications was used in the BRS capiaçu elephant grass subjected to five cutting intervals (30, 60, 90, 120 and 150 days) in the summer, making a total of 50 plots with a useful area of 4 x 2 m. There were daily increases in the dry matter production in the order of 382 kg ha-1, with 49,859 kg ha-1 being produced at 150 regrowth days. The dry matter content and organic matter linearly increased (P < 0.01) at different regrowth ages. The crude protein content, in vitro dry matter digestibility and in vitro digestibility of neutral detergent fiber linearly reduced (P < 0.01) 0.037%, 0.196% and 0.256% per day, respectively. Potential degradability of dry matter decreased from 68.9% at 30 days to 44.7% at 150 regrowth days (0.194 percentage units per day). The rate of degradation of fraction B 'c' was not modified (P = 0.94), averaging 1.46% hour-1. In the cultivation of BRS capiaçu elephant grass in the summer season, in the northern region of Minas Gerais, the age for harvesting between 90 and 120 days of regrowth is recommended.

scholarly journals Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation

2005 ◽  
Vol 48 (spe) ◽  
pp. 161-168 ◽  
Author(s):  
Lúcia de Fátima Araújo ◽  
Ariosvaldo Nunes Medeiros ◽  
Américo Perazzo Neto ◽  
Líbia de Sousa Conrado Oliveira ◽  
Flávio Luiz Honorato da Silva
Keyword(s):  
Dry Matter ◽  
Solid Medium ◽  
Animal Feed ◽  
In Vitro Digestibility ◽  
Cactus Pear ◽  
Protein Supplements ◽  
Dry Matter Digestibility ◽  
Matter Production ◽  
Gross Energy

The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross energy value when compared to that of the cactus pear in natura.

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