scholarly journals Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1605 ◽  
Author(s):  
Jing-Li Zhao ◽  
Meiping Zhang ◽  
Hong-Li Zhou
Keyword(s):  
Panax Ginseng ◽  
Bactericidal Activity ◽  
Acid Content ◽  
Uronic Acid ◽  
Hot Water ◽  
Microwave Assisted Extraction ◽  
Hot Water Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Uronic Acid Content

Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low. Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods—hot water extraction and microwave-assisted extraction—and compare their chemical composition and structure. In addition, their antibacterial and antioxidant activities were also determined. The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively. Moreover, the preliminary characterization of polysaccharides was identified after purification. The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra. Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content. More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content. Besides, both of them exhibited high bactericidal activity. These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.

Microwave-Assisted Extraction and In Vitro Antioxidant Evaluation of Polysaccharides from Enteromorpha prolifera

2011 ◽  
Vol 79 ◽  
pp. 204-209 ◽  
Author(s):  
Bin Wang ◽  
Guo Zhong Tong ◽  
You Le Qu ◽  
Li Li
Keyword(s):  
Microwave Power ◽  
Radical Scavenging ◽  
Hot Water ◽  
Butylated Hydroxytoluene ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Enteromorpha Prolifera ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Liquid Material

An efficient microwave-assisted extraction (MAE) technique was developed to extract polysaccharides from Enteromorpha prolifera (PEP). The operating parameters, such as microwave power, liquid/material ratio, temperature and extraction time, were optimized using orthogonal array design coupled with single factor method. PEP yield was determined by the phenol-sulfuric acid method. The optimum extraction conditions were determined as follows: microwave power, 700 W; liquid/material ratio, 40:1 (mL/g); temperature, 70 °C; and extraction time, 25 min. Under such conditions, PEP yield reached to 10.79 %. In comparison with conventional hot water extraction and ultrasonic-assisted extraction, MAE showed obvious advantages in terms of high extraction efficiency, saving energy, rapidity, solvent consumption, and so on. The data demonstrated that MAE could be a fast and reliable method for quantitative analysis of PEP. The scavenging capability of PEP to DPPH/hydroxyl radical reached to 65.2 % and 41.2 % at the concentration of 0.5 mg/mL. The reducing power of PEP was 0.354. Compared with butylated hydroxytoluene (BHT) and gallic acid (GA), the experimental results showed that DPPH radical scavenging activity of PEP was higher than that of BHT. Thus, PEP had good potential as a natural antioxidant used in functional food or medicine industries.

scholarly journals Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from Evodia lepta (Spreng) Merr. by Different Extraction Methods

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1842
Author(s):  
Feng Xiong ◽  
Hui-Xian Liang ◽  
Zhi-Jing Zhang ◽  
Taifo Mahmud ◽  
Albert S. C. Chan ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Structural Characteristics ◽  
Extraction Methods ◽  
Hot Water ◽  
Microwave Assisted Extraction ◽  
Antitumor Activities ◽  
Traditional Chinese Herbal Medicine ◽  
Microwave Assisted ◽  
Assisted Extraction

Evodia lepta (E. lepta) is a traditional Chinese herbal medicine with various biological activities. One of the active components of this widely used medicinal plant is believed to be an oligosaccharide. The extraction yields, structural characteristics, antioxidant, and antitumor activities of four oligosaccharide extracts obtained by hot water extraction (HEO), ultrasound-assisted extraction (UEO), enzyme-assisted (EEO), and microwave-assisted extraction (MEO) were investigated. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS), X-ray diffraction (XRD), and Scanning electron microscopy (SEM) results indicated that the extraction methods had a difference on the molecular mass distribution, structure, and morphology of the EOs. In addition, HEO and MEO showed strong antioxidant activities, which might be related to their uronic acid and protein contents. More interestingly, MEO was more active toward MDA-MB-231 cells compared to other cells, and cell growth inhibition was proposed to occur through apoptosis. Overall, microwave-assisted extraction is a promising technique for the extraction of high quality EO.

scholarly journals Analysis of ginsenosides in Panax ginseng in high pressure microwave-assisted extraction

2008 ◽  
Vol 110 (1) ◽  
pp. 161-167 ◽  
Author(s):  
Yutang Wang ◽  
Jingyan You ◽  
Yong Yu ◽  
Chenling Qu ◽  
Huarong Zhang ◽  
...  
Keyword(s):  
High Pressure ◽  
Panax Ginseng ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction

scholarly journals Ekstraksi Polisakarida Sulfat dari Sargassum polycystum dengan Metode Microwave Assisted Extraction dan Uji Toksisitasnya

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Riong Seulina Panjaitan ◽  
Lidya Natalia
Keyword(s):  
Fourier Transform ◽  
Brine Shrimp ◽  
Uronic Acid ◽  
Artemia Salina ◽  
Microwave Assisted Extraction ◽  
Brine Shrimp Lethality ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ft Ir ◽  
Uv Visible

Sargassum polycystum merupakan alga cokelat penghasil polisakarida sulfat yang memiliki aktivitas farmakologi yang menjanjikan. Selama ini metode ekstraksi polisakarida sulfat masih bersifat konvensional (seperti maserasi, sokletasi, dan refluks) yang memerlukan waktu ekstraksi relatif lama (4-6 jam). Penelitian ini bertujuan untuk mengekstraksi polisakarida sulfat menggunakan metode Microwave Assisted Extraction (MAE) serta melakukan uji toksisitasnya. Karakterisasi ekstrak polisakarida sulfat menggunakan spektrofotometer UV-Visible dan Fourier Transform Infrared (FT- IR). Uji toksisitas terhadap larva Artemia salina Leach dilakukan dengan metode Brine Shrimp Lethality Test (BSLT). Hasil ekstraksi polisakarida sulfat diperoleh rendemen sebesar 3,42±0,01% dimana tidak jauh berbeda dari metode konvensional. Hasil perhitungan bobot susut pengeringan polisakarida sulfat 7,60±0,085%. Karakterisasi polisakarida sulfat dengan spektrofotometer UV- Visible diperoleh panjang gelombang optimum pada 270 nm. Hasil spektrum FT-IR terdapat gugus fukosa yang dihubungkan oleh gugus sulfat dan O-Sulfat yang mengindikasikan adanya fukoidan. Selain itu masih ditemukan peregangan ester C=O yang menunjukan adanya asam uronat. Asam tersebut merupakan penyusun alginat dimana bukan merupakan polisakarida sulfat. Dari uji toksisitas diperoleh nilai LC50 polisakarida sulfat terhadap larva A. salina Leach sebesar 113,11 mg/mL yang tergolong kategori toksik. Ekstraksi polisakarida sulfat menggunakan MAE dapat mempercepat waktu ekstraksi dibandingkan dengan metode konvensional, tetapi perlu dilakukan tahap selanjutnya untuk menghilangkan senyawa pengotor lainnya seperti asam uronat. ABSTRACTSargassum polycystum, a brown algae, produces sulfate polysaccharides that have promising pharmacological activity. To date, the extraction of sulfate polysaccharide is still conventional (such as maceration, shoxletation and reflux) and requires a relatively long time (4-6 h). This study aims to extract the sulfate polysaccharides using the Microwave Assisted Extraction (MAE) and assess the toxycity. Sulfate polysaccharide extract was characterized by UV-Visible and Fourier Transform Infrared (FT-IR) spectrophotometer and tested for its toxicity against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. The yield of sulfate polysaccharide extraction was 3.42±0.01% which is not much different from conventional methods, but the advantage of the MAE method is in terms of time efficiency. The result of the calculation of the drying shrinkage weight of the sulfate polysaccharide is 7.60±0.085%. Characterization of sulfate polysaccharide using a UV-Visible spectrophotometer obtained an optimum wavelength at 270 nm. Based on spectra of FT-IR, it was identified that there are fucose groups which are connected by sulfate and O-Sulfate groups which indicates the presence of fucoidan. In addition, there was still a stretch of ester C=O which indicates the presence of uronic acid. The acid is a constituent of alginate which is not a sulfate polysaccharide. The LC50 value of sulfate polysaccharide against A. salina Leach larvae was 113.11 mg/mL which was classified as toxic. The extraction of sulfate polysaccharides using MAE can speed up the extraction time compared to conventional method, but a further step is needed to remove other impurities such as uronic acid

Determination of Trigonelline, Nicotinic Acid, and Caffeine in Yunnan Arabica Coffee by Microwave-Assisted Extraction and HPLC with Two Columns in Series

2012 ◽  
Vol 95 (4) ◽  
pp. 1138-1141 ◽  
Author(s):  
Hongcheng Liu ◽  
Jinliang Shao ◽  
Qiwan Li ◽  
Yangang Li ◽  
Hong Mei Yan ◽  
...  
Keyword(s):  
Particle Size ◽  
Nicotinic Acid ◽  
Correlation Coefficients ◽  
Hot Water ◽  
Microwave Assisted Extraction ◽  
Arabica Coffee ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
The Matrix ◽  
In Series

Abstract A simple, rapid method was developed for simultaneous extraction of trigonelline, nicotinic acid, and caffeine from coffee, and separation by two chromatographic columns in series. The trigonelline, nicotinic acid, and caffeine were extracted with microwave-assisted extraction (MAE). The optimal conditions selected were 3 min, 200 psi, and 120°C. The chromatographic separation was performed with two columns in series, polyaromatic hydrocarbon C18 (250 × 4.6 mm id, 5 μm particle size) and Bondapak NH2 (300 × 3.9 mm id, 5 μm particle size). Isocratic elution was with 0.02 M phosphoric acid–methanol (70 + 30, v/v) mobile phase at a flow rate of 0.8 mL/min. Good recoveries and RSD values were found for all analytes in the matrix. The LOD of the three compounds was 0.02 mg/L, and the LOQ was 0.005% in the matrix. The concentrations of trigonelline, nicotinic acid, and caffeine in instant coffee, roasted coffee, and raw coffee (Yunnan Arabica coffee) were assessed by MAE and hot water extraction; the correlation coefficients between concentrations of the three compounds obtained were close to 1.

Orthogonal test in optimizing microwave-assisted extraction process of magnolin from Magnoliae Flos

2010 ◽  
Vol 30 (5) ◽  
pp. 567-568
Author(s):  
Xiao-li LI ◽  
Ming-yuan ZHANG ◽  
Wei-quan ZHAO ◽  
Man Li ◽  
Hai-ying TENG ◽  
...  
Keyword(s):  
Extraction Process ◽  
Orthogonal Test ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction
Sign in / Sign up

Export Citation Format

Share Document