scholarly journals Composition and Antibacterial Activity of Aronia melanocarpa (Michx.) Elliot, Cornus mas L. and Chaenomeles superba Lindl. Leaf Extracts

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2011 ◽  
Author(s):  
Magdalena Efenberger-Szmechtyk ◽  
Agnieszka Nowak ◽  
Agata Czyżowska ◽  
Alicja Z. Kucharska ◽  
Izabela Fecka
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Hydroxycinnamic Acid ◽  
Total Phenolic ◽  
Lag Phase ◽  
Leaf Extracts ◽  
Acinetobacter Baumanii ◽  
Aronia Melanocarpa ◽  
Cornus Mas ◽  
Hydroxycinnamic Acid Derivatives

The purpose of this study was to investigate the composition of leaf extracts from Aronia melanocarpa, Chaenomeles superba, and Cornus mas, and their antimicrobial activity against typical spoilage-causing and pathogenic bacteria found in meat and meat products. The highest total phenolic content (TPC) was detected in C. superba extract, followed by C. mas and A. melanocarpa extracts. The antioxidant capacity of the extracts was measured by DPPH and ABTS assays. The lowest IC50 values were found for C. superba extract, followed by C. mas and A. melanocarpa extracts. LC-MS and HPLC analysis revealed that A. melanocarpa and C. superba extracts contained hydroxycinnamic acid derivatives and flavonoids (mainly flavonols). Hydroxycinnamic acid derivatives were detected in the C. mas extract, as well as flavonols, ellagitannins, and iridoids. The antibacterial activity of the plant extracts was tested against Gram-negative bacteria (Moraxella osloensis, Pseudomonas fragi, Acinetobacter baumanii, Escherichia coli, Enterobacter aerogenes, Salmonella enterica) and Gram-positive bacteria (Enterococcus faecium, Staphylococcus aureus, Brochothrix thermosphacta, Lactobacillus sakei, Listeria monocytogenes) using the microculture method. The extracts acted as bacteriostatic agents, decreasing the growth rate (µmax) and extending the lag phase (tlag). C. mas showed most potent antibacterial activity, as confirmed by principal component analysis (PCA).

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRESH AND FREEZE-DRIED SELECTED FRUIT JUICES

2017 ◽  
Vol 10 (9) ◽  
pp. 156 ◽  
Author(s):  
Naritsara Toobpeng ◽  
Pannapa Powthong ◽  
Pattra Suntornthiticharoen
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Total Phenolic ◽  
Zone Of Inhibition ◽  
Schleichera Oleosa ◽  
Freeze Dried

  Objective: The objective of this investigation was to assess the biological activity of fresh fruit juices and freeze dried fruit juices including antioxidant and antibacterial activity.Methods: The fruits used in this study were Schleichera oleosa (Lour.) Oken, Carissa carandas L., and Sandoricum koetjape (Burm.f.) Merr. The fresh juice sample (FJS) was extracted and also prepared for freeze drying sample (FDS) by freeze dryer. The antioxidant capacity of FJS and FDS was assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays and determination for total phenolic contents (TPCs) by Folin–Ciocalteu’s reagent. Antimicrobial tests were carried out by agar diffusion methods and evaluated by measuring the zone of inhibition against 10 isolates of pathogenic bacteria.Results: The highest antioxidant activity of FJS and FDS was found in S. oleosa and related to the scavenging effect on DPPH radical of FJS (100% concentration) and FDS (200 mg/ml) was found in the value of 93.05±0.31% and 82.18±0.33%, respectively. TPC of FJS and FDS from S. oleosa at the same concentration as in the DPPH assay was 1,003.53±1.96 ug GAE/300 g of matter and 827.77±23.15 ug GAE/100 ml of matter, respectively. Both FJS and FDS revealed antibacterial activity. The zone of inhibition of FJS was ranging from 8.3±0.6 to 24.0±1.0 mm and FDS values of 7.3±0.5-21.0±0.9 mm. The most potent sample against bacteria was FJS and FDS from S. oleosa which FJSs against Escherichia coli ESBL and FDS against Pseudomonas aeruginosa MDR2.Conclusion: Fruit juices of S. oleosa, C. carandas, and S. koetjape associated with antioxidant activity in the form of both fresh fruit juices and FDS. The result of this study showed that the value of DPPH and TPCs were slightly decreased in FDS compare with fresh juice. Screening of FJS and FDS of C. carandas revealed broad spectrum antibacterial activity. However, FJS of S. oleosa and S. koetjape showed inhibition of growth of bacteria, but few of FDS from these fruits could inhibit limited bacterial isolates. For antibacterial activity against pathogenic bacteria, the samples of fresh and freeze dried exhibited a different degree of activity.

scholarly journals Unraveling the selective antibacterial activity and chemical composition of citrus essential oils

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Carmen M. S. Ambrosio ◽  
Natália Y. Ikeda ◽  
Alberto C. Miano ◽  
Erick Saldaña ◽  
Andrea M. Moreno ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Negative Impact ◽  
Lactobacillus Rhamnosus ◽  
Normal Growth ◽  
Lag Phase ◽  
Beneficial Bacteria ◽  
Phase Duration ◽  
Minor Compounds

AbstractPost-weaning diarrhea (PWD) is an often disease affecting piglets. It is caused mainly by enterotoxigenic Escherichia coli (ETEC) colonization in pig gut. Antibiotics has been used to prevent, combat and control PWD and its negative impact on the productivity of pig breeding sector. Nonetheless, antibiotics due to their wide antibacterial spectrum also can reach beneficial gut bacteria, such as Lactobacillus. Lately, essential oils (EOs) have emerged as a potential alternative to using antibiotics in animal breeding because of their effect on bacterial growth. Commonly, citrus EOs are by-products of food industry and the availability of these EOs in the worldwide market is huge. Thus, six commercials citrus EOs were evaluated on ETEC strains, as model of pathogenic bacteria, and on Lactobacillus species, as models of beneficial bacteria. In overall, citrus EOs exhibited a selective antibacterial activity with higher effect on pathogenic bacteria (ETECs) than beneficial bacteria (Lactobacillus). Brazilian orange terpenes (BOT) oil presented the highest selective performance and caused higher disturbances on the normal growth kinetic of ETEC than on Lactobacillus rhamnosus. The action was dose-dependent on the maximal culture density (A) and the lag phase duration (λ) of the ETEC. The highest sub-inhibitory concentration (0.925 mg/mL) extended the λ duration to ETEC eight times (14.6 h) and reduced A in 55.9%. For L. rhamnosus, the λ duration was only extended 1.6 times. Despite the fact that limonene was detected as the major compound, the selective antibacterial activity of the citrus EOs could not be exclusively attributed to limonene since the presence of minor compounds could be implicated in conferring this feature.

scholarly journals Antibacterial Activities of Xanthium Strumarium L.

2018 ◽  
Vol 29 ◽  
pp. 70-77 ◽  
Author(s):  
Anjana Devkota ◽  
Ritu Kumari Das
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Xanthium Strumarium ◽  
Distilled Water ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Antibacterial activities of Xanthium strumarium L. (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. The antibacterial activity was studied against six pathogenic bacteria, three gram negative: Klebsiella pneumoniae (ATCC 15380), Proteus mirabilis (ATCC 49132), Escherichia coli (ATCC 25922) and three gram positive: Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25932) at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ ml, 250 mg/ml) of leaf extracts of X. strumurium. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antibacterial activity of extracts was determined by disc diffusion method and zone of inhibition (ZOI) was measured. Gram negative bacteria was found more resistant than gram positive bacteria. The most susceptible bacterium was S. aureus while the most resistant bacterium was E. coli. Methanolic extract was found more effective than distilled water. These findings suggest that extracts obtained from leaves of X. strumurium possess biobactericidal potential, which can suitably be exploited for making antibacterial drugs.J. Nat. Hist. Mus. Vol. 29, 2015, Page: 70-77

scholarly journals Gc-Ms Analysis and Antibacterial Activity of Mangosteen Leaf Extracts against Plant Pathogenic Bacteria

2016 ◽  
Vol 07 (07) ◽  
pp. 1013-1020 ◽  
Author(s):  
Qamar Mohammed Naji Alsultan ◽  
Kamaruzaman Sijam ◽  
Tavga Sulaiman Rashid ◽  
Khairulmazmi Bin Ahmad
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extracts ◽  
Plant Pathogenic Bacteria ◽  
Ms Analysis

scholarly journals Phenolic Compounds of Mountain Tea from the Balkans: LC/DAD/ESI/MSn Profile and Content

2011 ◽  
Vol 6 (1) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Jasmina Petreska ◽  
Gjose Stefkov ◽  
Svetlana Kulevanova ◽  
Kalina Alipieva ◽  
Vassya Bankova ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chemical Constituents ◽  
Neutral Loss ◽  
Principal Component ◽  
Hydroxycinnamic Acid ◽  
Flavonoid Glycosides ◽  
Total Phenolic ◽  
Phenylethanoid Glycosides ◽  
The Balkans ◽  
Hydroxycinnamic Acid Derivatives

Twenty-one samples of Sideritis species ( S. scardica, S. raeseri, S. taurica, S. syriaca and S. perfoliata) from various locations on the Balkan Peninsula were evaluated for their chemical constituents. Chemical analyses were focused on secondary metabolites, particularly phenolic compounds, which have several roles in the plant physiological processes and have demonstrated significant health beneficial effects. The occurrence of hydroxycinnamic acids, phenylethanoid glycosides and flavonoids has been investigated in taxonomically related taxa of the genus Sideritis. A systematic method for phenolic compounds identification was developed using tandem mass spectrometry coupled to high performance liquid chromatography with diode array detection. Scanning for precursor ions of commonly found phenolics in Sideritis species using LC/MSn with an ion trap instrument permitted the specific determination of hydroxycinnamic acid derivatives, and phenylethanoid and flavonoid glycosides. Further characterization of each phenolic compound was performed using MS/MS production analysis and common–neutral-loss analysis. This online technique allowed identification of three hydroxycinnamic acid derivatives, eight phenylethanoid glycosides, and twenty-four flavonoid glycosides. All the taxa analysed produced very similar phenolic patterns characterized by the presence of 5-caffeoylquinic acid, lavandulifolioside, verbascoside, hypolaetin 7- O-[6′”- O-acetyl]-allosyl(1→2)glucoside, apigenin 7-(4″- p-coumaroylglucoside), 4′- O-methylisoscutellarein 7- O-[6′”- O-acetyl]-allosyl(1→2)glucoside, and minor amounts of isoverbascoside, apigenin 7- O-allosyl(1→2)glucoside, isoscutellarein 7- O-allosyl-(1→2)-[6′- O-acetyl]-glucoside, hypolaetin 7- O-allosyl-(1→2)-[6″- O-acetyl]-glucoside and 4′- O-methylhypolaetin 7- O-[6′”- O-acetyl]-allosyl-(1→2)-[6′- O-acetyl]-glucoside. These results show that the investigated species are systematically very closely related. Phenylethanoid glycosides and flavonoid acetylglycosides are dominant and constitute 90% of the total phenolic compounds compared with hydroxycinnamic acid and flavonoid 7- O-glycosides. Principal component analysis (PCA) was performed for the nature and content of the different compounds to be correlated to the particular Sideritis species and also to the locations.

scholarly journals Aronia melanocarpa (Michx.) Elliot, Chaenomeles superba Lindl. and Cornus mas L. Leaf Extracts as Natural Preservatives for Pork Meat Products

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3009
Author(s):  
Magdalena Efenberger-Szmechtyk ◽  
Ilona Gałązka-Czarnecka ◽  
Anna Otlewska ◽  
Agata Czyżowska ◽  
Agnieszka Nowak
Keyword(s):  
High Throughput Sequencing ◽  
Modified Atmosphere Packaging ◽  
Meat Products ◽  
Storage Period ◽  
Rrna Gene ◽  
Pork Meat ◽  
Leaf Extracts ◽  
Aronia Melanocarpa ◽  
Cornus Mas ◽  
Natural Preservatives

The aim of this study was to investigate the possibility of using Aronia melanocarpa, Chaenomeles superba, and Cornus mas leaf extracts as natural preservatives for pork meat products. Pork sausages were stored in modified atmosphere packaging (MAP) (80% N2 and 20% CO2) at 4 °C for 29 days. The total psychrotrophic counts (TPC) were determined during the storage period, along with the numbers of Enterobacteriaceae and lactic acid bacteria (LAB). The extracts improved the microbial quality of the meat products but to a lesser extent than sodium nitrate (III). They reduced the amounts of Enterobacteriaceae and LAB. The A. melanocarpa leaf extract showed the strongest preservative effect. The bacterial biodiversity of the meat products was investigated based on high-throughput sequencing of the 16S rRNA gene. Two predominant bacteria phyla were identified, Proteobacteria and Firmucutes, mostly consisting of genera Photobacterium, Brochothrix, and Carnobacterium. The extracts also influenced microbial community in sausages decreasing or increasing bacterial relative abundance. The extracts significantly inhibited lipid oxidation and improved the water-holding capacity of the meat, with C. superba extract showing the strongest influence. In addition, A. melanocarpa and C. superba improved the redness (a*) of the sausages. The results of this study show that A. melanocarpa, C. superba, and C. mas leaf extracts can extend the shelf life of meat products stored in MAP at 4 °C.

Antibacterial mechanisms of Aronia melanocarpa (Michx.), Chaenomeles superba Lindl. and Cornus mas L. leaf extracts

2021 ◽  
Vol 350 ◽  
pp. 129218
Author(s):  
Magdalena Efenberger-Szmechtyk ◽  
Agnieszka Nowak ◽  
Agata Czyżowska ◽  
Monika Śniadowska ◽  
Anna Otlewska ◽  
...  
Keyword(s):  
Leaf Extracts ◽  
Aronia Melanocarpa ◽  
Cornus Mas

scholarly journals Evaluation of the Phytochemical and Antibacterial characteristics of leaf extracts of Xanthium strumarium L. against Bacteria

2020 ◽  
Vol 11 (1) ◽  
pp. 725-729
Author(s):  
Antu Kurrey ◽  
Lata Sharma ◽  
Shishir Tiwari
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Herbal Medicines ◽  
Wild Plant ◽  
Xanthium Strumarium ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Maximum Inhibition

Plants are one of the large sources of herbal medicines. Many plants have the capability to produce some bioactive constituents which give defense against microorganisms like bacteria and fungi and also from insects. Xanthium strumarium L. is a wild plant that grows like a weed. Leaves of  Xanthium strumarium L. were subjected for extraction in 4 different solvents viz. methanol, chloroform, aqueous, and ethanol. The aim of this study was to investigate the phytochemical screening and antimicrobial activity of methanol, chloroform, aqueous, and ethanol extracts prepared from leaves of  Xanthium strumarium L. The antibacterial activity was assessed by using the agar well diffusion assay against tested bacterial strain, Escherichia coli (CGSC 4312) and Bacillus subtilis (ATCC 9634). Phytochemical analysis has revealed that  Xanthium strumarium L. has most of the significant phytoconstituents like Saponins, Terpenoids, Flavanoids, Phenol, steroids, Anthraquinones, Tannins,  Alkaloids,  Glycosides, and Carbohydrate. The zone of inhibition was measured and compared by standard antibiotic streptomycin. The outcome of the present work showed that chloroform extract (400µg/ml) showed maximum inhibition against E. Coli (23mm), and Ethanol extract(400µg/ml) showed maximum inhibition against Bacillus subtilis (24mm). The result divulged the presence of antibacterial activity of different extracts of Xanthium strumarium L.against human pathogenic bacteria. Thus, this plant has many active compounds that can be used for the development of various potent drugs.

scholarly journals Antioxidant Activity and Antibacterial Effects on Clinical IsolatedStreptococcus suisandStaphylococcus intermediusof Extracts from Several Parts ofCladogynos orientalisand Their Phytochemical Screenings

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Pongtip Sithisarn ◽  
Piyanuch Rojsanga ◽  
Patchima Sithisarn ◽  
Sumet Kongkiatpaiboon
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
High Performance ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Spectrophotometric Methods ◽  
Staphylococcus Intermedius

Thein vitroantioxidant and antibacterial assays against clinically isolatedStreptococcus suisandStaphylococcus intermediusof the extracts prepared by decoction and ethanolic reflux of different parts of Chettaphangki (Cladogynos orientalisZipp. ex Span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and disc diffusion method were conducted. Quantitative analysis of total phenolic and total flavonoid contents in the extracts using spectrophotometric methods was also performed. Finally, phytochemical screening by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was conducted. Leaf ethanolic reflux extract (100 g) contained the highest total phenolic and total flavonoid contents of 7.21 ± 0.28 μg gallic acid equivalent (GAE) and 11.51 ± 2.02 μg rutin equivalent (RE), respectively. Chettaphangki extracts promoted low antioxidant activity with EC50values in the range of 0.27–0.48 mg/mL. Extracts and fractions from the roots and stems of this plant promoted low to intermediate antibacterial activity againstS. intermediuswith the inhibition zones between 7 and 14 mm. The chromatographic data suggested that the leaf extracts ofC. orientaliscontained rutin while the root and stem extracts contained scopoletin and chettaphanin I. Rutin promoted strong antioxidant activity while chettaphanin I showed low antibacterial activity againstStaphylococcus intermedius.

scholarly journals Assessment of Total Phenolic, Antioxidant, and Antibacterial Activities ofPassifloraSpecies

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Shiamala Devi Ramaiya ◽  
Japar Sidik Bujang ◽  
Muta Harah Zakaria
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Phenolic Antioxidant ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Methanol Extracts

This study focused on total phenolic content (TPC) and antioxidant and antibacterial activities of the leaves and stems ofPassiflora quadrangularis,P. maliformis, andP. edulisextracted using three solvents: petroleum ether, acetone, and methanol. The maximum extraction yields of antioxidant components from the leaves and stems were isolated using methanol extracts ofP. edulis(24.28%) andP. quadrangularis(9.76%), respectively. Among the leaf extracts, the methanol extract ofP. maliformishad the significantly highest TPC and the strongest antioxidant activity, whereas among the stem extracts, the methanol extract ofP. quadrangularisshowed the highest phenolic amount and possessed the strongest antioxidant activity. The antibacterial properties of thePassifloraspecies were tested using the disc diffusion method against 10 human pathogenic bacteria. The largest inhibition zone was observed for the methanol extract ofP. maliformisagainstB. subtilis. Generally, extracts from thePassifloraspecies exhibit distinct inhibition against Gram-positive but not Gram-negative bacteria. Based on the generated biplot, three clusters of bacteria were designated according to their performance towards the tested extracts. The present study revealed that methanol extracts of thePassifloracontain constituents with significant phenolic, antioxidant, and antibacterial properties for pharmaceutical and nutraceutical uses.

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