scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRESH AND FREEZE-DRIED SELECTED FRUIT JUICES

2017 ◽  
Vol 10 (9) ◽  
pp. 156 ◽  
Author(s):  
Naritsara Toobpeng ◽  
Pannapa Powthong ◽  
Pattra Suntornthiticharoen
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Total Phenolic ◽  
Zone Of Inhibition ◽  
Schleichera Oleosa ◽  
Freeze Dried

  Objective: The objective of this investigation was to assess the biological activity of fresh fruit juices and freeze dried fruit juices including antioxidant and antibacterial activity.Methods: The fruits used in this study were Schleichera oleosa (Lour.) Oken, Carissa carandas L., and Sandoricum koetjape (Burm.f.) Merr. The fresh juice sample (FJS) was extracted and also prepared for freeze drying sample (FDS) by freeze dryer. The antioxidant capacity of FJS and FDS was assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays and determination for total phenolic contents (TPCs) by Folin–Ciocalteu’s reagent. Antimicrobial tests were carried out by agar diffusion methods and evaluated by measuring the zone of inhibition against 10 isolates of pathogenic bacteria.Results: The highest antioxidant activity of FJS and FDS was found in S. oleosa and related to the scavenging effect on DPPH radical of FJS (100% concentration) and FDS (200 mg/ml) was found in the value of 93.05±0.31% and 82.18±0.33%, respectively. TPC of FJS and FDS from S. oleosa at the same concentration as in the DPPH assay was 1,003.53±1.96 ug GAE/300 g of matter and 827.77±23.15 ug GAE/100 ml of matter, respectively. Both FJS and FDS revealed antibacterial activity. The zone of inhibition of FJS was ranging from 8.3±0.6 to 24.0±1.0 mm and FDS values of 7.3±0.5-21.0±0.9 mm. The most potent sample against bacteria was FJS and FDS from S. oleosa which FJSs against Escherichia coli ESBL and FDS against Pseudomonas aeruginosa MDR2.Conclusion: Fruit juices of S. oleosa, C. carandas, and S. koetjape associated with antioxidant activity in the form of both fresh fruit juices and FDS. The result of this study showed that the value of DPPH and TPCs were slightly decreased in FDS compare with fresh juice. Screening of FJS and FDS of C. carandas revealed broad spectrum antibacterial activity. However, FJS of S. oleosa and S. koetjape showed inhibition of growth of bacteria, but few of FDS from these fruits could inhibit limited bacterial isolates. For antibacterial activity against pathogenic bacteria, the samples of fresh and freeze dried exhibited a different degree of activity.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

2021 ◽  
Vol 3 (2) ◽  
pp. 53-61
Author(s):  
Angga Crystal Loasana Yami ◽  
Irmanida Batubara ◽  
Kholis Abdurachim Audah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Ic50 Value ◽  
Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

scholarly journals Assessment of Total Phenolic, Antioxidant, and Antibacterial Activities ofPassifloraSpecies

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Shiamala Devi Ramaiya ◽  
Japar Sidik Bujang ◽  
Muta Harah Zakaria
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Phenolic Antioxidant ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Methanol Extracts

This study focused on total phenolic content (TPC) and antioxidant and antibacterial activities of the leaves and stems ofPassiflora quadrangularis,P. maliformis, andP. edulisextracted using three solvents: petroleum ether, acetone, and methanol. The maximum extraction yields of antioxidant components from the leaves and stems were isolated using methanol extracts ofP. edulis(24.28%) andP. quadrangularis(9.76%), respectively. Among the leaf extracts, the methanol extract ofP. maliformishad the significantly highest TPC and the strongest antioxidant activity, whereas among the stem extracts, the methanol extract ofP. quadrangularisshowed the highest phenolic amount and possessed the strongest antioxidant activity. The antibacterial properties of thePassifloraspecies were tested using the disc diffusion method against 10 human pathogenic bacteria. The largest inhibition zone was observed for the methanol extract ofP. maliformisagainstB. subtilis. Generally, extracts from thePassifloraspecies exhibit distinct inhibition against Gram-positive but not Gram-negative bacteria. Based on the generated biplot, three clusters of bacteria were designated according to their performance towards the tested extracts. The present study revealed that methanol extracts of thePassifloracontain constituents with significant phenolic, antioxidant, and antibacterial properties for pharmaceutical and nutraceutical uses.

scholarly journals Phytochemical Content, Antioxidant, Alpha-Glucosidase Inhibitory and Antibacterial Activities of Spineless Cactus Pear Cultivars

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1312
Author(s):  
Mologadi B. Mabotja ◽  
Sonja L. Venter ◽  
Christian P. Du Plooy ◽  
Tukayi Kudanga ◽  
Stephen O. Amoo
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Cactus Pear ◽  
Phytochemical Content ◽  
E Coli ◽  
Alpha Glucosidase ◽  
Phenolic And Flavonoid

Variation in cultivars can influence plant biological activities. This study aimed to identify superior cultivars while determining the variability in the phytochemical content, antioxidant, alpha-glucosidase inhibitory and antibacterial activities of cladode extracts from selected spineless Burbank cactus pear (Opuntia ficus-indica and Opuntia robusta) cultivars. Total phenolic and flavonoid contents were determined using the Folin-Ciocalteu and aluminum chloride spectrophotometric methods, respectively. Antioxidant activity was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene linoleic acid assays. Alpha-glucosidase inhibition was determined using a spectrophotometric method and antibacterial activity using a non-polar (petroleum ether) and polar (50% methanol) extracts against two Gram-positive and two Gram-negative bacteria. Significant variation in phytochemical content, antioxidant, antidiabetic and antibacterial activities was observed amongst the cultivars. Alpha-glucosidase inhibitory activity varied widely with IC50 values ranging from 0.06 to 1.85 mg/mL. Radical scavenging activity of Polypoly cultivar was about seven fold higher than that recorded in other cultivars with low activity. Turpin and Berg x Mexican cultivars had the highest total phenolic and flavonoid contents, whilst the non-polar extract of Turpin also exhibited higher antibacterial activity against Bacillus subtilis and Escherichia coli. Sicilian Indian Fig was amongst the cultivars with a higher antioxidant activity, whilst also showing a strong inhibition against B. subtilis and E. coli. Polypoly cultivar demonstrated strong antioxidant and antidiabetic activities while its polar extract showed the highest total antibacterial activity against B. subtilis. The cultivar Malta was superior in terms of its antibacterial potency and efficacy against B. subtilis, Staphylococcus aureus and E. coli. The potential of using spineless cactus pear cladodes as a functional food with antioxidant, antidiabetic and antibacterial properties against pathogenic food spoilage bacteria in place of synthetic compounds was established. The significance of cultivar selection to increase this potential was highlighted.

scholarly journals Phytochemical Composition, Antioxidant and Antibacterial Activities of Extracts from Different Parts of Brocchia cinerea (Vis.)

2021 ◽  
Vol 12 (4) ◽  
pp. 4432-4447
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Pharmaceutical Products ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Different Parts

Brocchia cinerea (Vis.) is an Asteraceae-family plant widely used in traditional medicine in Southeastern Morocco to treat several ailments. The aqueous extracts of flowers, leaves, stems and aerial parts of B. cinerea were subjected to a preliminary phytochemical screening test for various constituents. The polyphenols, flavonoids, and tannins contents were determined. Moreover, the antioxidant activity was evaluated by DPPH scavenging assay and ferric reduction antioxidant power. However, the antibacterial activity was evaluated against six pathogenic bacteria. Phytochemical screening of different parts of B. cinerea revealed alkaloids, flavonoids, saponins, tannins, and terpenoids. The leaves extract showed the highest polyphenols concentration (27.15±0.92 mg GAE/g of Ext) and flavonoids (17.46±0.66 mg QE/g of Ext), while flowers extract was highest in tannin (9.57±1.24 mg TAE/g of Extract). All extracts of B. cinerea showed moderate to high antioxidant power, among which the leaves extract demonstrated the strongest antioxidant activity, with an IC50 value of 0.99 mg/mL and an optical density of 1.15. In the case of antibacterial activity screening, leaves extract showed the highest inhibition zone diameters ranging from 9 to 27 mm against the tested bacteria. The results demonstrate that the aqueous extracts of different parts of B. cinerea, especially the leaves, could be developed as pharmaceutical products.

scholarly journals Characterization of Curcuminoid from Curcuma xanthorrhiza and Its Activity Test as Antioxidant and Antibacterial

Molekul ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 79
Author(s):  
Sri Atun ◽  
Nurfina Aznam ◽  
Retno Arianingrum ◽  
Senam Senam ◽  
Bian Ihda An Naila ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Activity Test ◽  
Medium Activity ◽  
Main Component

Curcuminoids are the main component found in many Zingiberaceae family plants. The aim of this study was to characterize curcuminoid and its activity test as an antioxidant and antibacterial. Dryed powder of C. xanthorrhiza (1 kg) was macerated with ethanol for 24 hours at room temperature. Ethanol extract of C. xanthorrhiza was subsequently fractionated with n-hexane and chloroform to take the yellow or orange indicated contain of curcuminoids. Analysis of total phenolic levels was carried out by the Follin-Ciaocalteau method. The isolation of curcuminoid componens from this fraction  was carried out by chromatographic method and the structure elucidation was performed by interpretation of spectroscopic data, including UV, IR, 1H and 13C NMR 1D and 2D. The antioxidant activity test used the DPPH (2,2-diphenylpicrylhydrazyl) method, while the antibacterial activity test used Kirby Bauer test diffusion method. The results showed that the curcuminoid fraction yield was 10.06%  from ethanol extract C. xanthorrhiza. The total phenolic content of curcuminoids fraction was 745.45 ± 18.5 mg galic acid (GA)/g extract. Curcuminoids fraction was isolated a known compound desmethoxycurcumin (1). The content of demethoxycurcumin (1) in curcuminoid fraction is 20.97 %.The antioxidant activity of curcuminoids fraction showed strongest activity with IC50 24.98 µg/ml. Antibacterial activity against of the four pathogenic bacteria showed medium activity. The study suggests that curcuminoids extract from C. xanthorrhiza rhizome have potential compounds could be suitable for antioxidant and the treatment of various infections caused of microbial.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

scholarly journals ANTIBACTERIAL ACTIVITY OF METHANOLIC RHIZOME EXTRACT OF ALPINIA CALCARATA ROSC. (ZINGIBERACEAE)

2016 ◽  
Vol 3 (1) ◽  
pp. 65-66
Author(s):  
Arumugasamy K ◽  
Nantha Kumar R ◽  
Abdul Kaffoor H ◽  
Shalimol A
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Bacterial Activity ◽  
Proteus Vulgaris ◽  
Bacterial Strains ◽  
Zone Of Inhibition

The methanolic rhizome extract of A. calcarata was evaluated for its antibacterial activities against five bacterial strains Pseudomonas aeuroginosa, Proteus vulgaris, Salmonella paratyphi, Bacillus thurungiensis and Staphylococcus faccealis. The extract has inhibited all the tested bacterial species with different manner at various concentration. However the higher level zone of inhibition in 400 (mg/ml) is significant against all the above said bacterial strains of these Salmonella paratyphi. Based on the present study it can be conculuded that the plant rhizome possess potent anti bacterial activity.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF BIXIN PIGMENT FROM ANNATTO (Bixa orellana L.) SEEDS

2010 ◽  
Vol 7 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Pipin T. Kurniawati ◽  
H. Soetjipto ◽  
Leenawati Limantara
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bixa Orellana ◽  
Isolation And Identification ◽  
Double Beam ◽  
Layer Chromatography

Research on Bixa orellana L. have been done to isolate, identify and determine bixin percentage, the antioxidant and antibacterial activities of bixin from B. orellana seed.  Isolation and identification of bixin was done by thin layer chromatography (TLC), column chromatography, chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin was calculated by JECFA method, the antioxidant activity was determined by DPPH (1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed by the use of agar diffusion method. Thin layer chromatography (TLC) for the crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has 75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas the antibacterial activity of bixin against the Escherichia coli and Staphylococus aureus could be classified as weak inhibition category at 500-750 μg and medium inhibition category at 1500 μg.   Keywords: Bixa orellana L., bixin, antioxidant, antibacteria

scholarly journals Study of Biological Activity and Chemical Constituent of Annona Reticulata

2016 ◽  
Vol 21 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Bimala Subba ◽  
Pramod Aryal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Column Chromatography ◽  
Authentic Sample ◽  
Chemical Constituent ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Ic50 Value ◽  
Annona Reticulata

The methanol and hexane extract of leaf of Annona reticulata, vernacularly known as ‘Sareefa’ were subjected to biological assay and column chromatography respectively. Column chromatography of the hexane extract of the leaf of A. reticulata resulted in the isolation of one pure compound PF1. The compound PF1 was suggested as β-sitosterol by comparing TLC, IR and melting point with authentic sample. The antibacterial activity against two bacteria, Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) was done by agar well diffusion method and Zone of Inhibition (ZOI) was observed. The ZOI obtained ranged from 8 to 12 mm. The methanol extract of leaf showed moderate antibacterial activity on comparison with standard antibiotic streptomycin. The Antioxidant activity of the extract was tested using scavenging activity of DPPH (1, 1-Diphenyl-2- Picrylhydrazyl) radical method. The IC50 value of A. reticulata was found (41 µg/ml). The standard compound has shown IC50 value as 120 µg/ml. The antimicrobial and antioxidant activity of the extract was positively associated with the total phenolic and flavonoid contents of the extract. This study revealed that methanolic leaf extract of A. reticulata comprise effective potential source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.Journal of Institute of Science and TechnologyVolume 21, Issue 1, August 2016, page: 157-163

scholarly journals Effect of Freeze, Oven and Microwave Pretreated Oven Drying on Color, Browning Index, Phenolic Compounds and Antioxidant Activity of Hawthorn (Crataegus orientalis) Fruit

2018 ◽  
Vol 46 (2) ◽  
pp. 449-456 ◽  
Author(s):  
Hacer COKLAR ◽  
Mehmet AKBULUT ◽  
Semih KILINC ◽  
Ali YILDIRIM ◽  
Iliasu ALHASSAN
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Drying Methods ◽  
Drying Method ◽  
Microwave Pretreatment ◽  
Oven Drying ◽  
Freeze Dried

Flowers, leaves and fruits of hawthorn plant are traditionally used for treating diseases like hypertension and atherosclerosis. The medicinal effects of the plant are generally attributed to its phenolic compounds. However, the fruits are perishable materials because of their high content of water, and generally dried and stored to be used outside its season. The main aim of this research was to investigate the effect of different drying methods on phenolic compounds of the hawthorn fruit. Fruits were collected from the wild growing trees in Turkey. De-seeded fruits were dried in freeze-, oven- (60 oC) and microwave pretreated oven drying (microwave application for 5 min at 360 W before drying at 60 oC) methods and analyzed for antioxidant activity, phenolic compounds, total phenolic content and color parameters. Total phenolic content of fresh hawthorn fruits was found as 13.36 mg g-1 DW. Oven- and microwave pretreated oven drying methods had a reductive effect on total phenolic content and antioxidant activity of fruits when compared to freeze drying method. (-)-Epicatechin (994.10 mg kg-1 DW), rutin (765.30 mg kg-1 DW), and procyanidin B2 (553.80 mg kg-1 DW) were the main phenolics of the fruit. Lowest values of these three compounds were observed in oven-dried fruits. Microwave pretreatment oven drying method resulted in browner product. Although the highest phenolic concentration and antioxidant activity were occurred in freeze-dried sample, microwave pretreatment before oven drying could be applied to reduce the time and cost of drying in terms of phenolic compounds and antioxidant activity.

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