scholarly journals Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5517
Author(s):  
Patrycja Sowa ◽  
Dana Marcinčáková ◽  
Michał Miłek ◽  
Ewelina Sidor ◽  
Jaroslav Legáth ◽  
...  
Keyword(s):  
Real Time ◽  
Antioxidant Properties ◽  
Gastrointestinal Diseases ◽  
Epithelial Cell Line ◽  
Therapeutic Effects ◽  
Leaf Extracts ◽  
Spectrophotometric Methods ◽  
Ethanol Extracts ◽  
Polyphenolic Profile ◽  
Mts Assay

Plants from Asteraceae family are widely used for their therapeutic effects in the treatment of gastrointestinal diseases, but the consequences of excessive intake still need to be studied. The aims of this study were the evaluation of cytotoxicity, measurement of antioxidant properties and determination of polyphenolic profile of Tanacetum vulgare L. (tansy), Achillea millefolium L. (yarrow) and Solidago gigantea Ait. (goldenrod) ethanolic extracts. The cytotoxicity of extracts was monitored by xCELLigence system in real time by using porcine intestinal epithelial cell line (IPEC-1) and by measurement of changes in metabolic activity ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay). The antioxidant properties were measured by spectrophotometric methods and polyphenolic profiles were determined by HPLC-DAD for 50% ethanol extracts (10% w/v). Strong cytotoxic effect was recorded for tansy and yarrow extracts (125–1000 µg/mL) by xCELLigence system and MTS assay. Conversely, a supportive effect on cell proliferation was recorded for goldenrod extracts (125 µg/mL) by the same methods (p < 0.001). The antioxidant activity was in good correlation with total polyphenolic content, and the highest value was recorded for goldenrod leaves, followed by tansy leaves, goldenrod flowers and yarrow leaf extracts. The goldenrod extracts were abundant with flavonoids, whereas phenolic acid derivatives predominated in the polyphenolic profile of tansy and yarrow.

Organo-protective and antioxidant properties of leaf extracts of Syzygium guineense var macrocarpum against ferric nitriloacetate-induced stress of Wistar rats

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Francine Tankeu Nzufo ◽  
Constant Anatole Pieme ◽  
Jacques Romain Njimou ◽  
Prosper Cabral Biapa Nya ◽  
Bruno Moukette Moukette ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Leaf Extracts ◽  
Enzymatic Markers ◽  
Syzygium Guineense ◽  
Ethanol Extracts ◽  
Polyphenolic Profile

AbstractBackgroundThe present study focused on the antioxidant, phenolic profile and free radical scavenging-mediated protective effect of leaves extracts ofMethodsSpectrophotometric standardized methods were used to determine the free radical scavenging potential, antioxidant and protective properties of plant extracts on rat homogenates.ResultsAll the extracts showed a concentration-dependent free radical quenching potential, and the ability to protect all the tested organs by inhibiting the lipid peroxidation and potentiating or restoring the activity of enzymatic and non enzymatic markers. The polyphenolic profile revealed the presence of at least one simple phenolic acid (gallic, caffeic, para-coumaric acid) although the majority (6 out of 14) of the compounds used as standard are present in the aqueous and aqueous–ethanol extracts.ConclusionsEthanolic extract of leaves of

scholarly journals Evaluation of Phytochemical and Antioxidant Properties of 15 Italian Olea europaea L. Cultivar Leaves

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1998 ◽  
Author(s):  
Francesca Nicolì ◽  
Carmine Negro ◽  
Marzia Vergine ◽  
Alessio Aprile ◽  
Eliana Nutricati ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Olea Europaea ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Total Phenolic ◽  
Therapeutic Effects ◽  
Leaf Extracts ◽  
Olea Europaea L ◽  
Olea Europaéa

Olive leaf extracts are of special interest due to their proven therapeutic effects. However, they are still considered a by-product of the table olive and the oil industries. In order to learn possible ways of exploiting this waste for health purposes, we investigated the phytochemical profiles and antioxidant activities in the leaves of 15 Italian Olea europaea L. cultivars grown in the same pedoclimatic conditions. The phenolic profiles and amounts of their seven representative compounds were analyzed using HPLC ESI/MS-TOF. The antioxidant activities were determined using three different antioxidant assays (DPPH, ORAC, and superoxide anion scavenging assay). Wide ranges of total phenolic content (11.39–48.62 g GAE kg−1 dry weight) and antioxidant activities (DPPH values: 8.67–29.89 µmol TE mg−1 dry weight, ORAC values: 0.81–4.25 µmol TE mg−1 dry weight, superoxide anion scavenging activity values: 27.66–48.92 µmol TE mg−1 dry weight) were found in the cultivars. In particular, the cultivars Itrana, Apollo, and Maurino, showed a high amount of total phenols and antioxidant activity, and therefore represent a suitable natural source of biological compounds for use in terms of health benefits.

scholarly journals Biological Activity of Blackcurrant Extracts (Ribes nigrumL.) in Relation to Erythrocyte Membranes

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Dorota Bonarska-Kujawa ◽  
Sylwia Cyboran ◽  
Romuald Żyłka ◽  
Jan Oszmiański ◽  
Halina Kleszczyńska
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Erythrocyte Membrane ◽  
Membrane Lipids ◽  
Membrane Surface ◽  
Antioxidant Properties ◽  
Erythrocyte Membranes ◽  
Leaf Extracts ◽  
Hydrophobic Region ◽  
Spectrophotometric Methods

Compounds contained in fruits and leaves of blackcurrant (Ribes nigrumL.) are known as agents acting preventively and therapeutically on the organism. The HPLC analysis showed they are rich in polyphenol anthocyanins in fruits and flavonoids in leaves, that have antioxidant activity and are beneficial for health. The aim of the research was to determine the effect of blackcurrant fruit and leaf extracts on the physical properties of the erythrocyte membranes and assess their antioxidant properties. The effect of the extracts on osmotic resistance, shape of erythrocytes and hemolytic and antioxidant activity of the extracts were examined with spectrophotometric methods. The FTIR investigation showed that extracts modify the erythrocyte membrane and protect it against free radicals induced by UV radiation. The results show that the extracts do not induce hemolysis and even protect erythrocytes against the harmful action of UVC radiation, while slightly strengthening the membrane and inducing echinocytes. The compounds contained in the extracts do not penetrate into the hydrophobic region, but bind to the membrane surface inducing small changes in the packing arrangement of the polar head groups of membrane lipids. The extracts have a high antioxidant activity. Their presence on the surface of the erythrocyte membrane entails protection against free radicals.

scholarly journals Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

2020 ◽  
Vol 10 (03) ◽  
pp. 109-115
Author(s):  
Shreya Udaya ◽  
Nishith Babu ◽  
Dechamma Pandyanda Nanjappa ◽  
Krithika Kalladka ◽  
Gunimala Chakraborty ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Stem Bark ◽  
Oxygen Species ◽  
Cassia Fistula ◽  
Potential Applications ◽  
Ethanol Extracts ◽  
Stress Models

Abstract Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish. Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark. Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

Investigation of antioxidant activity and analysis of phenolic compounds of some Asteraceae plants by HPLC: A comparison between Methanol and Ethanol extracts

2021 ◽  
Vol 02 ◽  
Author(s):  
Donia Anvari ◽  
Rashid Jamei
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Sinapic Acid ◽  
Total Phenolic ◽  
Therapeutic Effects ◽  
Antioxidant Power ◽  
Antioxidant Agents ◽  
Ethanol Extracts

Background: Medicinal and healing plants have been used in treating human diseases for centuries because of their therapeutic effects. They may assist in curing common ailments such as a multitude of skin problems, various disorders from muscle spasms to cuts and wounds. They can be used to relieve symptoms of different illnesses from a cold to some forms of arthritis or some allergies as well. The Asteraceae plant is a strong source of antimicrobial and antioxidant agents and this paper focuses on its specifications. Objectives: This study aimed to investigate the antioxidant potential and radical scavenging of different solvents (Methanol and Ethanol) of five species, i.e., Artemisia absinthium L., Arctium lappa L., Centaurea cyanus L., Silybum marianum L., and Echinops ritro L., belonging to the Asteraceae family. Methods: Methanol and ethanol extracts of the above plants were prepared. The obtained sections were evaluated for total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), chain-breaking activity (CBA), thiobarbituric acid (TBA), and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Also, Ferric reducing antioxidant power (FRAP), Nitric oxide (NO) radical scavenging, Hydrogen peroxide (H2O2) radical scavenging, and Superoxide (O2-) radical inhibition assay were measured. Phenolic compounds were determined and measured by high-performance liquid chromatography (HPLC) as well. Results: The collected and analyzed data showed that the highest values for the TPC, TFC, TBA, and DPPH were related to methanol extract of A. lappa L. Moreover, the maximum values for the CBA, H2O2, and O2- were observed in the Ethanol extract of E. ritro L., while methanol extract of E. ritro L. showed the highest amount of FRAP and NO. Eventually, the highest value for TAC was related to A. absintium L., and it was also realized that methanol compared to ethanol solvent was more successful in the extraction procedure. Conclusions: These findings suggest that A. lappa L. and E. ritro L. extracts can be considered excellent natural antioxidant agents. The type of solvent can affect the extraction of phenolic compounds. Sinapic acid, as the highest level of phenolic acid, was found in S. marianum L.

scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

scholarly journals In Vitro Assessment of the Antioxidant Properties of Aqueous Byproduct Extracts of Vitis vinifera

2019 ◽  
Vol 57 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Roberto Puglisi
Keyword(s):  
Plasma Membranes ◽  
Antioxidant Properties ◽  
Dermal Fibroblasts ◽  
Total Phenolic ◽  
Mitochondrial Activity ◽  
Cabernet Sauvignon ◽  
Leaf Extracts ◽  
Volume Fractions ◽  
Positive Effects

Aqueous extracts were obtained at low temperature with the Naviglio technology from grapevine stalks (Merlot), marc (Merlot and Cabernet Sauvignon) and leaves (Merlot) as typical byproducts of winemaking industry, and their properties were evaluated cytofluorometrically on human dermal fibroblasts. Leaf extracts had the greatest total phenolic ((47.6±3.5) mg/g) and proanthocyanidin ((24.2±0.1) mg/g) contents compared to the others. The preliminary colorimetric MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) assay individuated two consecutive non-toxic volume fractions of each extract (from 0.8 to 12.8 %) that were adopted for three cytofluorometric tests. The first cell membrane test did not evidence any harmful effects against plasma membranes at the two non-toxic volume fractions. The second mitochondrial membrane test showed a decreased (p&lt;0.01) percentage of cells ((15.7±8.3) vs (32.5±1.3) %) with active polarized mitochondrial membranes at the higher non-cytotoxic volume fractions of extracts from Cabernet Sauvignon marc in response to 4.5 mM H2O2, and from Merlot stalks (p&lt;0.05) at 1.5 mM H2O2 ((49.3±6.1) vs (64.6±2.4) %) and without H2O2 ((89.7±2.4) vs (96.9±1.8) %), compared to the controls submitted to the same H2O2 concentration. Conversely, mitochondrial activity of leaf extracts significantly (p&lt;0.05) increased ((96.3±1.8) and (96.4±1.4) %) after treatment with 0.5 mM H2O2 at both non-cytotoxic volume fractions compared to control ((88.2±1.1) %). Finally, as evidenced by the third oxidative status test, stalk extracts did not evidence relevant effects on the cellular oxidative state, while the extracts of marc and leaves demonstrated significantly medium (p&lt;0.05) to highly (p&lt;0.001) positive effects following exposure to H2O2 ranging from 0.5 to 4.5 mM, compared to controls.

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