scholarly journals Investigation of the Phytochemical Composition, Antioxidant Activity, and Methylglyoxal Trapping Effect of Galega officinalis L. Herb In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5810
Author(s):  
Katarzyna Bednarska ◽  
Piotr Kuś ◽  
Izabela Fecka
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Positive Control ◽  
Hot Water ◽  
Array Detector ◽  
In Vitro Tests ◽  
Phytochemical Analysis ◽  
Ionization Mass ◽  
Galega Officinalis

Galega officinalis L. has been known for centuries as an herbal medicine used to alleviate the symptoms of diabetes, but its comprehensive chemical composition and pharmacological activity are still insufficiently known. The current study involved the qualitative and quantitative phytochemical analysis and in vitro evaluation of the antioxidative and methylglyoxal (MGO) trapping properties of galega herb. Ultra high-performance liquid chromatography coupled with both the electrospray ionization mass spectrometer and diode-array detector (UHPLC-ESI-MS and UHPLC-DAD) were used to investigate the composition and evaluate the anti-MGO capability of extracts and their components. Hot water and aqueous methanol extracts, as well as individual compounds representing phytochemical groups, were also assessed for antioxidant activity using DPPH (2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) assays. Quercetin and metformin were used as a positive control. We confirmed the presence of tricyclic quinazoline alkaloids, guanidines, flavonoids, and hydroxycinnamic acids (HCAs) in galega extracts. The polyphenolic fraction was dominated by mono-, di-, and triglycosylated flavonols, as well as monocaffeoylhexaric acids. The in vitro tests indicated which G. officinalis components exhibit beneficial antioxidative and MGO trapping effects. For galega extracts, flavonols, and HCAs, a potent antiradical activity was observed. The ability to trap MGO was noted for guanidines and flavonoids, whereas HCA esters and quinazoline alkaloids were ineffective. The formation of mono-MGO adducts of galegine, hydroxygalegine, and rutin in the examined water infusion was observed.

scholarly journals Activity-Guided Fractionation of Red Fruit Extracts for the Identification of Compounds Influencing Glucose Metabolism

Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1166 ◽  
Author(s):  
Johanna Josefine Ostberg-Potthoff ◽  
Kirsten Berger ◽  
Elke Richling ◽  
Peter Winterhalter
Keyword(s):  
Glucose Metabolism ◽  
High Performance ◽  
Punica Granatum ◽  
Positive Control ◽  
In Vitro Tests ◽  
Fruit Extracts ◽  
Aronia Melanocarpa ◽  
Red Grape ◽  
Punica Granatum L

An activity-guided search for compounds influencing glucose metabolism in extracts from aronia (Aronia melanocarpa, A.), pomegranate (Punica granatum L., P.), and red grape (Vitis vinifera, RG) was carried out. The three extracts were fractionated by means of membrane chromatography to separate the anthocyanins from other noncolored phenolic compounds (copigments). In addition, precipitation with hexane was performed to isolate the polymers (PF). The anthocyanin and copigment fractions (AF, CF) of aronia, pomegranate, and red grape were furthermore fractionated with high-performance countercurrent chromatography (HPCCC) and the subfractions were characterized by HPLC-PDA-MS/MS analyses. Each of the (sub-)fractions was examined by in vitro-tests, i.e., the inhibition of the activity of α-amylase and α-glucosidase. On the basis of this screening, several potent inhibitors of the two enzymes could be identified, which included flavonols (e.g., quercetin), ellagitannins (e.g., pedunculagin), and anthocyanins (e.g., delphinidin-3-glucoside and petunidin-3-glucoside). In the α-glucosidase assay all of the examined fractions and subfractions of the fruit extracts were more active than the positive control acarbose.

scholarly journals Neuroprotective and Antioxidant Enhancing Properties of Selective Equisetum Extracts

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2565
Author(s):  
Denisa Batir-Marin ◽  
Monica Boev ◽  
Oana Cioanca ◽  
Cornelia Mircea ◽  
Ana Flavia Burlec ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Chemical Characterization ◽  
Biological Action ◽  
Neuroprotective Activity ◽  
In Vitro Tests ◽  
Skin Conditions ◽  
Tract Infections

The sterile stems belonging to the Equisetum species are often used in traditional medicine of various nations, including Romanians. They are highly efficient in treating urinary tract infections, cardiovascular diseases, respiratory tract infections, and medical skin conditions due to their content of polyphenolic derivatives that have been isolated. In this regard, this study aimed to provide the chemical composition of the extracts obtained from the Equisetum species (E. pratense, E. sylvaticum, E. telmateia) and to investigate the biological action in vitro and in vivo. For the chemical characterization of the analyzed Equisetum species extracts, studies were performed by using ultra-high-performance liquid chromatography (UHPLC-DAD). In vitro evaluation of the antioxidant activity of the plant extracts obtained from these species of Equisetum genus was determined. The neuroprotective activity of these three ethanolic extracts from the Equisetum species using zebrafish tests was determined in vivo. All obtained results were statistically significant. The results indicate that E. sylvaticum extract has a significant antioxidant activity; whereas, E. pratense extract had anxiolytic and antidepressant effects significantly higher than the other two extracts used. All these determinations indicate promising results for the antioxidant in vitro tests and neuroprotective activity of in vivo tests, particularly mediated by their active principles.

scholarly journals Free and Conjugated Phenolic Profiles and Antioxidant Activity in Quinoa Seeds and Their Relationship with Genotype and Environment

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1046
Author(s):  
Fabiana Antognoni ◽  
Giulia Potente ◽  
Stefania Biondi ◽  
Roberto Mandrioli ◽  
Lorenzo Marincich ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Environmental Conditions ◽  
High Performance ◽  
Biological Activities ◽  
Chenopodium Quinoa ◽  
Northern Italy ◽  
Temperate Climate ◽  
Total Phenolic ◽  
Array Detector

The nutraceutical interest in quinoa (Chenopodium quinoa Willd.) seeds is associated with the presence of macronutrients, micronutrients, minerals, vitamins, and polyphenols. In particular, polyphenols contribute to the health-promoting effects of this food crop, and their levels are influenced by environmental conditions. Production of quinoa is recently being explored in temperate climate areas, including Italy. The aim of this research was to assess the profile of bioactive compounds in seeds of two quinoa varieties, Regalona-Baer and Titicaca, grown in northern Italy, compared to that of seeds of those varieties grown in Chile and Denmark, respectively. High-performance liquid chromatography-diode array detector (HPLC-DAD) analysis of phenolic acid and flavonoid profiles, both in their free and soluble conjugated forms, showed that the main differences between Regalona grown in Chile and Italy were for the free vanillic acid and daidzein contents, while the two Titicaca samples mainly differed in quercetin derivative levels. The total phenolic index was comparable in Titicaca and Regalona, and only a slight decrease in this parameter was found in seeds of the two varieties grown in Italy. The in vitro antioxidant activity of seed extracts, evaluated by means of three different assays, indicated that it correlated with flavonol (quercetin derivative) levels. In conclusion, the results indicate that, although environmental conditions alter the polyphenolic profile and biological activities, it is possible to grow good-quality quinoa in northern Italy.

scholarly journals Evaluation of Antioxidant and Antihyperhipidemic Activity of Pterospermum acerifolium (Sterculiaceae) In Vitro

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Hemlata Hemlata ◽  
Asija Rajesh ◽  
Charanjeet Singh ◽  
Kumawat Radhey Shyam
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
High Performance ◽  
Cardiac Glycosides ◽  
Research Work ◽  
Computer Assisted ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Antihyperlipidemic Activity

The objective of present research work was to determine the antioxidant and antihyperlipidemic activity in the leaves of Pterospermum acerifolium belonging to family Sterculiaceae. The phytochemical analysis of leaves of Pterospermum acerifolium showed the presence of anthraquinones, cardiac glycosides, flavonoids, saponins and tannins etc. Oxidative compounds are responsible for prognosis of many diseases like Alzheimer’s disease, ischemic heart disease, Parkinson disease, hyperlipidemia and atherosclerosis. Antioxidants are the compounds responsible for curing such ailments that are formed by oxidative stress to the cells. Antioxidant activity of bark of Pterospermum acerifolium was determined by 1, 1- diphenyl-2-picrylhydrazyl radical (DPPH). Ascorbic acid and finofibrate was used as standard drug and antihyperlipidemic activity was determine by computer-assisted high-performance liquid chromatography and determine the various types of lipoprotein like LDL, VLDL and HDL.

scholarly journals Evaluation of in vitro antioxidant activity and HPTLC finger printing analysis of Physalis peruviana fruits

2020 ◽  
pp. 384-389
Author(s):  
Saratha Venkatesan ◽  
Ambiga Somasundaram ◽  
Sangavi Rengaraj
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Research Work ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Physalis Peruviana ◽  
Reducing Ability ◽  
Finger Printing ◽  
In Vitro Antioxidant Activity

In this research work to evaluate in vitro antioxidant activity and HPTLC finger printing analysis of Physalis peruviana fruits. The chemical fingerprinting was carried out by high performance thin layer chromatography.  It was carried out by the CAMAG HPTLC system equipped with Linomat V sample applicator, twin through plate development chamber, TLC scanner III and integration software WIN CATS-4.02. Physalis peruviana fruit extract was tested for phytochemical screening and in vitro anti-oxidant enzymes like 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH), total antioxidant activity and reducing ability. Physalis peruviana fruit extract effectively scavenged free radicals at all different concentrations and showed its potent antioxidant activity. Phytochemical analysis revealed the presence of various major phytoconstituents like alkaloids, flavonoids, saponins, phenolics, tannins and anthraquinones. The HPTLC fingerprint qualitatively revealed predominant amount of quercetin. Physalis peruviana fruit extract will be subjected to further extensive studies to isolate and identify their active constituents which are useful for understanding their mechanism of action as antioxidants.

scholarly journals Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Ayinuer Reheman ◽  
Haji Akber Aisa ◽  
Qing Ling Ma ◽  
Dilaram Nijat ◽  
Rahima Abdulla
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Traditional Medicine ◽  
Gallic Acid ◽  
High Performance ◽  
Radical Scavenging ◽  
Array Detector ◽  
Chemical Fingerprinting ◽  
Orbitrap Ms

By merging a high-performance liquid chromatography diode array detector (HPLC-DAD) method with high-performance thin-layer chromatography (HPTLC), an assay was developed for chemical fingerprinting and quantitative analysis of traditional medicine Majun Mupakhi ELA (MME), and constituent compounds were identified using HPLC coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS method. In addition, the antioxidant capacity of MME was assessed based on the ability of components to scavenge radicals using in vitro method. Using a HPLC-DAD method with HPTLC easily validated the chemical fingerprinting results and quantified three characteristic components, namely, gallic acid (1), daidzein (2), and icariin (3), in commercial MMEs. The three compounds presented excellent regression values (R2=0.9999) in the ranges of the test and the method recovery was in the range from 100.49% to 100.68%. The fingerprints had 27 common characteristic peaks, of which 13 were verified by rapid UHPLC-DAD-Q-Orbitrap-MS analysis. In vitro antioxidant assays rapidly assessed and contrasted antioxidant activity or the free radical scavenging activity of the main polyphenolic classes in MMEs, and the antioxidant capacity was mostly affected by the presence of gallic acid. Thus, this study establishes a powerful and meaningful approach for MME quality control and for assessing in vitro antioxidant activity.

scholarly journals Phytochemical evaluation, HPTLC analysis and in-vitro antioxidant activity of hydroalcoholic leaf extract of Grewia hirsuta collected from Western Ghats forest

2020 ◽  
Vol 11 (3) ◽  
pp. 4653-4659
Author(s):  
Dattatraya Kature ◽  
Gaurav Gupta ◽  
Ritu Gilotra
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Western Ghats ◽  
High Performance ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
In Vitro Antioxidant Activity

In-vitro antioxidant action of hydroalcoholic leaf extract of Grewia hirsuta (HAEGH) has been examined using one, “1-diphenyl-2-picryl-hydrazil (DPPH) free from radical scavenging” actions. The plant collected from the forest of the Western Ghats region of Karnataka province. The motive for plant collection from a specific location is the plant of forests exhibits the variation in growth, quantity, and quality of their active ingredients and secondary metabolites due to influence ecological factors like effect changes in location, soil, climate, etc. The work corresponds to preliminary phytochemical investigation for diverse phytoconstituents and quantitative phytochemical analysis of total phenolic, flavonoids & alkaloids content (TPC, TFC & TAC respectively) was evaluated with advanced methods. “HPTLC (High performance thin-layer” chromatography) fingerprint investigation was achieved for qualitative determination of the likely number of elements present in the hydroalcoholic extract. In-vitro antioxidant activity of HAEGH has been determined through hydroxyl radical scavenging assay that exhibited strong dose-dependent antioxidant activity as compared with standards compound, ascorbic acid. The IC50 value of HAEGH found, 25.90 % inhibition and for ascorbic acid, it was 17.68%. The Preliminary phytochemical estimation found presence of flavonoids, alkaloids, glycosides, phenol, proteins, diterpins and quantitative phytochemical analysis estimation of TPC, TFC & TAC found to be 3.627%, 4.059% & 5.671% respectively. HPTLC analysis of HAEGH at 354nm reveals the presence of a compound with Rf value 0.44 compare with Rf value 0.46 of quercetin. These outcomes indicated that the hydroalcoholic leaf extract of Grewia hirsuta plant contains phytoconstituents that exhibit antioxidant activity possible because of the existence of bioactive compounds.

scholarly journals Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

2021 ◽  
Author(s):  
Mariola Dreger ◽  
Katarzyna Seidler-Łożykowska ◽  
Milena Szalata ◽  
Artur Adamczak ◽  
Karolina Wielgus
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Array Detector ◽  
Reproduction Rate ◽  
Full Spectrum ◽  
Breeding Programs ◽  
Chamerion Angustifolium ◽  
Oenothein B

AbstractThe purpose of the study was to evaluate Chamerion angustifolium (L.) Holub genotypes for preliminary selection and further breeding programs aimed at obtaining a suitable industrial form for the pharmaceutical applications. Clonally propagated plants representing 10 genotypes of Ch. angustifolium were regenerated under in vitro conditions, hardened and planted in the field. Studies included an evaluation of shoot proliferation, phytochemical assessment of in vitro and ex vitro plants as well as investigations of intraspecies variability regarding four phenological stages: vegetative, beginning of blooming, full blooming, and green fruit phases. Quantitative and qualitative analyses of bioactive compounds were performed using high-performance liquid chromatography coupled with diode array detector and tandem mass spectrometer (HPLC–DAD–MS/MS) and high-performance liquid chromatography (HPLC) methods. The efficiency of shoot multiplication varied between genotypes from 8.12 to 21.48 shoots per explant. A high reproduction rate (> 20 shoots per explant) was recorded for four lines (PL_45, PL_44, PL_58, DE_2). Plants grown in vitro synthesized oenothein B (11.2–22.3 mg g−1 DW) and caffeic acid derivatives. Plants harvested from field contained the full spectrum of polyphenols characteristic for this species, and oenothein B and quercetin 3-O-glucuronide were the most abundant. The maximal content of oenothein B was determined in the vegetative phase of fireweed, while some flavonoids were found in the highest amount in full blooming phase. The results of analysis of variance indicated significant differences among genotypes in oenothein B, 3-O-caffeoylquinic acid and flavonoids accumulation in four phenological phases. PL_44 plants were characterized by high content of oenothein B and quercetin 3-O-glucuronide as well as a relatively high level of other flavonoids. Based on our phytochemical and micropropagation studies, PL_44 genotype was the best candidate for early selection and further breeding programs.

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