scholarly journals Antioxidant, Anti-Inflammatory, and Inhibition of Acetylcholinesterase Potentials of Cassia timoriensis DC. Flowers

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2594
Author(s):  
Maram B. Alhawarri ◽  
Roza Dianita ◽  
Khairul Niza Abd Razak ◽  
Suriani Mohamad ◽  
Toshihiko Nogawa ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Acetylcholinesterase Activity ◽  
Phytochemical Analysis ◽  
Methanol Extracts ◽  
Anti Inflammatory ◽  
Inhibitory Potential ◽  
Radical Scavenging Ability ◽  
First Time

Despite being widely used traditionally as a general tonic, especially in South East Asia, scientific research on Cassia timoriensis, remains scarce. In this study, the aim was to evaluate the in vitro activities for acetylcholinesterase (AChE) inhibitory potential, radical scavenging ability, and the anti-inflammatory properties of different extracts of C. timoriensis flowers using Ellman’s assay, a DPPH assay, and an albumin denaturation assay, respectively. With the exception of the acetylcholinesterase activity, to the best of our knowledge, these activities were reported for the first time for C. timoriensis flowers. The phytochemical analysis confirmed the existence of tannins, flavonoids, saponins, terpenoids, and steroids in the C. timoriensis flower extracts. The ethyl acetate extract possessed the highest phenolic and flavonoid contents (527.43 ± 5.83 mg GAE/g DW and 851.83 ± 10.08 mg QE/g DW, respectively) as compared to the other extracts. In addition, the ethyl acetate and methanol extracts exhibited the highest antioxidant (IC50 20.12 ± 0.12 and 34.48 ± 0.07 µg/mL, respectively), anti-inflammatory (92.50 ± 1.38 and 92.22 ± 1.09, respectively), and anti-AChE (IC50 6.91 ± 0.38 and 6.40 ± 0.27 µg/mL, respectively) activities. These results suggest that ethyl acetate and methanol extracts may contain bioactive compounds that can control neurodegenerative disorders, including Alzheimer’s disease, through high antioxidant, anti-inflammatory, and anti-AChE activities.

scholarly journals In vitro Antidiabetic, anti-obesity and antioxidant proprities of Rosemary extracts

2014 ◽  
Vol 10 (2) ◽  
pp. 2305-2316 ◽  
Author(s):  
Manel Ben Ali ◽  
Kais Mnafgui ◽  
Abdelfattah Feki ◽  
Mohamed Damak ◽  
Noureddine Allouche
Keyword(s):  
Essential Oil ◽  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Antioxidant Properties ◽  
Methanolic Extracts ◽  
Ic50 Values ◽  
Inhibitory Potential ◽  
Prevention Of Diabetes ◽  
First Time

Diabetes mellitus is a serious health problem worldwide that has adverse and long-lasting consequences for individuals, families, and communities. Hence, this study sought to investigate the inhibitory potential of rosemary extracts on key-enzymes related to diabetes such as α-amylase and pancreatic lipase activities, as well as to assess their antioxidant properties in vitro. The IC50 values of Rosemary essential oil, ethyl acetate and methanolic extracts against α-amylase were 28.36, 34.11 and 30.39 µg/mL respectively, and those against pancreatic lipase were 32.25, 36.64 and 34.07 µg/mL, suggesting strong anti-diabetic and anti-obesity effects of Rosemary. The methanolic extract was found to be the highest in levels of phenolic (282.98 µgGAE/mg extract) and flavonoids (161.05 µg QE /mg extract) contents as well as in the antioxidant activity (IC50 = 15.82 µg/mL) as compared to other extracts ethyl acetate (IC50 = 32.23 µg/mL) and essential oil  (IC50 = 96.12 µg/mL).Antioxidant efficacy of Rosemary extracts has been estimated in the stabilization of sunflower oil (SFO) at three different concentrations, i.e. 200 (SFO-200), 500 (SFO-500) and 1000 ppm (SFO-1000). Results showed the highest efficiency of SFO-1000.The results obtained in this study demonstrated for the first time that Rosemary is a potent source of natural inhibitors of α-amylase and pancreatic lipase with powerful antioxidants proprieties that might be used in the food stabilization and the prevention of diabetes and obesity complications as a complementary pharmacological drug.

scholarly journals ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA

2018 ◽  
Vol 10 (8) ◽  
pp. 36 ◽  
Author(s):  
Muhammad Dawood Shah ◽  
Mohammad Iqbal
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Palmitic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Hexadecanoic Acid ◽  
Methanol Extracts

Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained from Commelina nudiflora were investigated for the free radical scavenging effects and phytochemical analysis.Methods: The antioxidative effect of the essential oil, methanol extracts and methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively. The phytochemical analyses of the essential oil, methanol extracts and methanol fractions were performed by gas chromatography and mass spectrometry (GCMS). Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetate and chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoids and tannins etc. The identified bioactive constituents of essential oil, methanol extract and methanol fractions of C. nudiflora were indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl, phenol, benzyl alcohol, eugenol, phenol, 2, 4-bis (1,1-dimethylethyl), hexadecanoic acid, ethyl ester (palmitic acid ester), n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid) and phytol. All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractions of C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.

Pesudomance sp. Bacteria Associated with Marine Sponge as a Promising and Sustainable Source of Bioactive Molecules

2019 ◽  
Vol 20 (11) ◽  
pp. 964-984 ◽  
Author(s):  
Asmaa Nabil-Adam ◽  
Mohamed A. Shreadah ◽  
Nehad M. Abd El Moneam ◽  
Samy A. El-assar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Marine Sponge ◽  
Bacterial Strain ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bioactive Molecules ◽  
Phytochemical Analysis ◽  
Anti Inflammatory

Background: The study was conducted to identify the bacterial strain associated with marine sponge Hyrtiosaff. erectus collected from the Red Sea coastal water and to assess the utilization of their secondary metabolites for human benefit as antioxidant, anti-Alzheimer, anti-viral, anticancer and anti-inflammatory agent. Methods: After biochemical identification of Pesudomance sp. bacterial strain, the total polyphenol contents, cytotoxic, antioxidant, anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activity of the Pesudomance sp. ethyl acetate extract were investigated by applying different biochemical assays. Polyphenol contents were investigated using spectrophotometric techniques. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and 2,2/-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical scavenging activity assays. The cytotoxic effects were investigated by using the human cancerous cell lines. Results: The anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activities were determined using ELISA. Qualitative phytochemical analysis of the Pesudomance sp. extract demonstrated the presence of a large and diverse group of substances such as alkaloids, carbohydrates, flavonoids, phenols, terpenoids, saponins, and tannins. The strong antioxidant activity of the Pesudomance sp. extract was mainly attributed to the protective role of polyphenols against reactive oxygen. It was also observed that Pesudomance sp. extract possessed significant anti-Alzheimer activity with 94% at 1 mg. The extract showed also high antiviral activity (90%) using reverse transcriptase enzymes inhibition assay. The examination of the anticancer activity by applying two experimental models, i.e., PTK and SHKI cleared out high significant percentages of 76.19 and 83.09 %; respectively. Conclusion: The anti-inflammatory profiling using TNF, COX1, COX2, IL6 also revealed high antiinflammatory activity with different metabolic pathway of 62.70, 75.444, 79.27 and 54.15 %; respectively. The present study concluded that ethyl acetate extract of Pesudomance sp. possessed strong antioxidant, anti-Alzheimer, and anti-viral, anticancer and anti-inflammatory activities. Further studies are required to purify the bioactive compounds.

Chemical Composition, Antioxidant, and Anti-inflammatory Activities of Whole Parts of Onobrychis crista-galli (L.) Lam

2020 ◽  
Vol 10 (5) ◽  
pp. 642-654
Author(s):  
Wassila Benchadi ◽  
Hamada Haba ◽  
Emerson Ferreira Queiroz ◽  
Laurence Marcourt ◽  
Jean-Luc Wolfender ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Gallic Acid ◽  
Ethyl Acetate Extract ◽  
Fresh Weight ◽  
Anti Inflammatory ◽  
First Time ◽  
Β Carotene

Objective: The aim of the present study is to examine the phytochemical components and the biological activities of the whole parts of Onobrychis crista-galli (L.) Lam. growing in Algeria. Methods: The structures of the isolated compounds 1-15 were elucidated using different spectroscopic methods and by comparison with literature data. The biological evaluation of the plant was determined by the in vitro antioxidant and anti-inflammatory activities. The antioxidant activity of various extracts (petroleum ether, ethyl acetate, and n-butanol) and some isolated flavonoids was assessed by using five different test systems, namely, 1,1-diphenyl-2-picryl-hydrazil (DPPH), 2,2’- azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), cupric reducing antioxidant capacity (CUPRAC), superoxide alkaline DMSO, and β-carotene/linoleic acid tests. In addition, the total phenolic and flavonoid contents of the extracts were determined as gallic acid and quercetin equivalents, respectively. In vitro anti-inflammatory activity by protein denaturation was measured for all extracts. Results: Phytochemical investigation of the ethyl acetate and n-butanol extracts of Onobrychis crista- galli led to the isolation for the first time of fifteen known compounds. The present study reports for the first time the isolation and identification of fifteen known compounds from this species. The ethyl acetate extract had rich phenolic content indicating (31.09 ± 0.40 mg gallic acid equivalents/g of fresh weight), while n-butanol extract displayed a high content in flavonoid compounds (60.70±0.7 mg quercetin equivalents/ g of fresh weight). This investigation indicated that the ethyl acetate extract of O. crista-galli showed the highest antioxidant activity (IC50= 17.13±0.51 μg/mL, DPPH), (IC50= 82.99±2.50 μg/mL, ABTS), and (A0.50= 94.67±0.41 μg/mL, CUPRAC), (IC50= 97.09±2.20 μg/mL, DMSO), (IC50: 36.73±1.17 μg/mL, β-carotene/linoleic acid). Furthermore, the compound luteolin 5-methyl ether (14) exhibited a good antioxidant activity in DPPH (IC50= 06.05 ± 0.15 μg /mL) and CUPRAC (A0.5= 12.57 ± 0.34 μg /mL) assays. Moreover, the ethyl acetate and nbutanol extracts of O. crista-galli evidenced a good to moderate in vitro anti-inflammatory activity. Conclusion: The extracts of the whole plant of O. crista-galli (L.) Lam. showed potent antioxidant and anti-inflammatory activities.

In vitro antimicrobial activity and phytochemical analysis of fruits of Syzygium aromaticum (L.) Merr. & L.M.Perry - An important medicinal Plant

2016 ◽  
Vol 5 (4) ◽  
pp. 137-140
Author(s):  
Duraisamy Kayal Vizhi ◽  
◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam ◽  
Natarajan Nirmal Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Syzygium Aromaticum ◽  
Active Components ◽  
Methanol Extracts ◽  
Important Medicinal Plant

The fruit of Syzygium aromaticum is an important medicinal plant was subjected to phytochemical screening and antimicrobial effect of methanol, ethyl acetate and acetone extracts. Antimicrobial activity was analyzed by agar well diffusion method against gram positive and gram negative bacteria. The phytochemical analysis carried out revealed the presence of alkaloids, coumarins, catechins, flavanoids, phenols, saponins, terpenoids, tannins and steroids in various solvent extracts. Methanol extract of S. aromaticum showed good antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa and Staohylococcus aureus. It has been showed that the methanol extracts had wider range of activity on these organisms than the acetone and ethyl acetate extracts, which indicates that the methanol extracts of selected plants may contain the active components. The use of plant extracts with known antimicrobial properties can be of great significance in therapeutic treatments.

scholarly journals In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela

2016 ◽  
Vol 64 (4) ◽  
Author(s):  
Alexis Buitrago ◽  
Janne Del Carmen Rojas ◽  
Yonel Peñalosa
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Methanol Extracts ◽  
Phenol Derivatives ◽  
Methanolic Extracts ◽  
High Concentrations

Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. The aim of present study is to evaluate the free radical scavenging capacity, total phenolic and flavonoids content as well as the qualitative phytochemical screening of methanol extracts obtained from Vismia macrophylla (VM) and Vismia baccifera (VB) collected in Táchira and Mérida state, Venezuela. Phytochemical screening of VB and VM methanolic extracts carried out using various chemical assays revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH radical scavenging assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 µg/mL. Phenol and flavonoid assays also revealed that methanol extracts of both VM and VB contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in present investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT AND ANTIDIABETIC POTENTIALS OF DIFFERENT FRACTIONS OF MAYTENUS HEYNEANA ROOT EXTRACT

2019 ◽  
Vol 12 (1) ◽  
pp. 408
Author(s):  
Sumithira G ◽  
Senthil Kumar Gp
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Cation Radical ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Prevention Of Diabetes

Objective: In an attempt to explore herbal drug which may become useful in the prevention of diabetes and antioxidant potential by the ethanol extracts of Maytenus heyneana (MH) root belonging to the family Celastraceae and their different fractions were studied.Methods: Different fractionation was done using chloroform, ethyl acetate, and methanol on ethanolic extract of MH and preliminary phytochemical analysis was done by standard methods to identify the presence of important compounds. In vitro antioxidants activities were carried by 2,2-diphenyl- 2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation radical scavenging assays. For antidiabetic potential, α-amylase and α-glucosidase enzyme inhibitory studies were carried on different fractions.Results: Phytochemical studies show the presence of alkaloids, flavonoids, phenols, cardiac glycosides, and terpenoids in all fractionations; however, tannins and quinones were present in ethyl acetate fraction and saponins in methanolic fraction. For antioxidant activity, ethyl acetate fraction shows concentration of the sample causing 50% inhibition (IC50) values in 22.31 μg/ml and methanolic fraction shows in 12.82 μg/ml concentrations for DPPH and ABTS radical scavenging assay, respectively. In case of antidiabetic activity, methanolic fraction offered significant result in inhibitory action of α-glucosidase and also for α-amylase assay IC50 (5.28 and 3.14 μg/ml) than other fractions.Conclusion: From the results of our studies, it can be concluded that MH shows antidiabetic and antioxidant values and methanolic fraction of MH could be possessed potential constituents in the prevention of diabetes and antioxidant than other fractions. However, further studies are required to validate.

scholarly journals Tomorou attenuates progression of rheumatoid arthritis through alteration in ULK-1 independent autophagy pathway in collagen induced arthritis mice model

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Arooma Jannat ◽  
Peter John ◽  
Attya Bhatti ◽  
Muhammad Qasim Hayat
Keyword(s):  
Rheumatoid Arthritis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Treated Group ◽  
Phytochemical Analysis ◽  
Mice Model ◽  
Apoptosis Resistance ◽  
Autophagy Pathway ◽  
Anti Inflammatory

Abstract Rheumatoid arthritis (RA) is a multifactorial disease which is complicated by apoptosis resistance. Autophagy is one of the key mechanisms which are involved in the development of resistance to apoptosis as well as to the standard therapies against RA. Aberration in autophagy and apoptosis homeostasis results in the development of oxidative stress thus complicates the pathogenesis of RA. In the given study, tomorou, an indigenous herb of Hunza-Nagar Valley, has been evaluated for its pro-apoptotic, anti-inflammatory, and anti-rheumatic activity. Several major classes of bioactive phytochemicals including steroids, terpenoids, phenols, flavonoids, and essential oils have been detected in the aqueous and ethyl acetate extracts of tomorou through phytochemical analysis. Plant extracts depicted enhanced free radical scavenging activity through di-phenyl-2-picryl hydrazyl hydrate (DPPH) assay and ameliorated the symptoms of arthritis in collagen induced arthritic (CIA) mice model. Moreover, the 6 week extract treatment resulted in the reduction of IL-6 serum levels thus making it an effective anti-inflammatory agent. Upregulation of microtubule-associated proteins light chain 3b (LC3b) and downregulation of UNC51-like kinase 1 (ULK-1) in arthritic mice proposed a ULK-1 independent non-canonical autophagy pathway. Treatment with extracts upregulated the expression of caspase 3 which in turn inhibited the activity of LC3b thus altering the autophagy pathway. However, ULK-1 expression was restored to normal in aqueous extract treated group whereas it was upregulated in ethyl acetate extract treated group. On the other hand, a novel LC3b-independent autophagy pathway was observed in mice treated with ethyl acetate extract due to ULK-1 upregulation. Despite of significantly high IL-6 levels, the arthritic symptoms waned off which suggested the participation of IL-6 in LC3b-independent autophagy pathway in the extract prepared in ethyl acetate. Conclusively, the study established pro-apoptotic, antioxidant, anti-inflammatory and anti-rheumatic activity of tomorou and suggested an intricate autophagy pathway shift.

scholarly journals In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten.

2020 ◽  
Vol 12 (2) ◽  
pp. 222-232
Author(s):  
Turgut TASKIN ◽  
Murat DOGAN ◽  
Muhammet E. CAM ◽  
Talip SAHIN ◽  
Ismail SENKARDES
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Cytotoxic Activities ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Direct Methanol ◽  
Anti Inflammatory

Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts.  The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%).  According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.

scholarly journals Synthesis And Evaluation Of Herbal Formulation Using Natural Extract

2020 ◽  
Vol 11 (3) ◽  
pp. 3450-3458
Author(s):  
Sudha Rameshwari K ◽  
Muthu Muneeswari M ◽  
Gloria Jemmi Christobel R ◽  
Mallika R ◽  
Renuka R ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Phytochemical Analysis ◽  
Significant Activity ◽  
Herbal Formulation ◽  
Nitric Oxide Radical ◽  
Anti Inflammatory

Our study is intended to prepare and evaluate the polyherbal formulation (PHF) using Nyctanthes arbor-tristis, Piper nigrum, and lemon juice. Poly Herbal Formulation F1 and F2 were synthesised and subjected to qualitative, quantitative phytochemical analysis and antioxidant activity was measured using a standard protocol. Antimicrobial activity of PHFs was observed by the agar well diffusion method. The anti-inflammatory and anti-diabetic activities (In-vitro) were studied in F1 by using inhibition of albumin denaturation method. TLC and FTIR are done for the structural elucidation in F1. The F1 and F2 exhibited the presence of flavonoids, alkaloids, tannins, phenols, terpenoids, carbohydrates, saponins, Glycosides and proteins. The F1 and F2 show significant activity against the standard in the antimicrobial and antioxidant activity. The presence of flavonoids and alkaloids may contribute to the anti-microbial activity. The Nitric oxide radical scavenging activity and a metal chelating assay of the F1 possess the highest antioxidant activity of 73.1% and 69% respectively. TLC confirms the presence of flavonoids in F1. FTIR spectrum shows the functional group of amines, alcohols, nitrites, aromatic and aliphatic compounds. These FTIR bands denote stretching and vibrational bands responsible for the compounds like flavonoids, terpenoids, phenols and amino acids. This investigation shows the excellent result in antimicrobial, antioxidant and in-vitro anti-diabetic and anti-inflammatory activities. In future, the PHF of F1 can be used as potent therapeutical agents and can be further exploited for their other beneficial effect in the pharmacological studies.

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