scholarly journals Comparative Study on Seed Characteristics, Antioxidant Activity, and Total Phenolic and Flavonoid Contents in Accessions of Sorghum bicolor (L.) Moench

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3964
Author(s):  
Bimal-Kumar Ghimire ◽  
Ji-Won Seo ◽  
Chang-Yeon Yu ◽  
Seung-Hyun Kim ◽  
Ill-Min Chung
Keyword(s):  
Antioxidant Activity ◽  
Agronomic Traits ◽  
Germination Rate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Extensive Study ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Seed Characteristics ◽  
Seed Extracts

Sorghum is a major cereal food worldwide, and is considered a potential source of minerals and bioactive compounds. Its wide adaptive range may cause variations in its agronomic traits, antioxidant properties, and phytochemical content. This extensive study investigated variations in seed characteristics, antioxidant properties, and total phenolic (TPC) and flavonoid contents (TFC) of sorghum collected from different ecological regions of 15 countries. The antioxidant potential of the seed extracts of various sorghum accessions was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging assays. Significant variations in TPC were observed among the sorghum accessions. All 78 sorghum accessions used in this study exhibited significant variations in TFC, with the lowest and highest amount observed in accessions C465 and J542, respectively. DPPH scavenging potential of the seed extracts for all the accessions ranged from 11.91 ± 4.83 to 1343.90 ± 81.02 µg mL−1. The ABTS assay results were similar to those of DPPH but showed some differences in the accessions. Pearson’s correlation analysis revealed a wide variation range in the correlation between antioxidant activity and TPC, as well as TFC, among the sorghum accessions. A wide diversity range was also recorded for the seed characteristics (1000-seed weight and seed germination rate). A dendrogram generated from UPGMA clustering, based on seed traits, antioxidant activity, TPC, and TFC was highly dispersed for these accessions. Variations among the accessions may provide useful information regarding the phytoconstituents, antioxidant properties, and phytochemical contents of sorghum and aid in designing breeding programs to obtain sorghum with improved agronomic traits and bioactive properties.

scholarly journals In Vitro Antioxidant and Antiproliferative Efficacy of Abrus Precatorius Seed Extracts on Hep2c Cells and its Inhibitory Efficacy with Receptors of Cervical Carcinoma

2022 ◽  
Author(s):  
Amritpal Kaur ◽  
Yash Sharma ◽  
Kamran Waidha ◽  
Madhumita P Ghosh ◽  
Anoop Kumar ◽  
...  
Keyword(s):  
Tannic Acid ◽  
Herbal Remedy ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Seed Extract ◽  
Total Phenolic ◽  
Cervix Carcinoma ◽  
Abrus Precatorius ◽  
Ic50 Value ◽  
Seed Extracts

Abstract Abrus precatorius is a widely distributed tropical medicinal plant with several therapeutic properties, however its seed extract has not been studied against cervical cancer (CaCx) till date. Herein, we have assessed the antioxidant and antiproliferative properties of A. precatorius seed extracts (ethyl acetate and 70% ethanol) prepared from different extraction methods (Soxhlet and maceration) against human cervix carcinoma cells (Hep2C). We observed a significantly higher total flavonoid content of APE (sox) i.e.,112.7±0.127 mg Quercetin Equivalent/g of extract than others; total phenolic content of APA (mac) seed extract was higher i.e.,108.53±0.089 mg Quercetin Equivalent/g of extract and total tannin content of APA (sox) was higher i.e., 98.98± 0.011 mg tannic acid equivalents/g of extract. In addition, tannic acid, rutin and piperine were identified in extracts by HPLC. Furthermore, APA (sox) exhibited the highest radical scavenging activity with an IC50 value of 14.49±0.93µg/ml. APE (mac) showed the most significant antiproliferative activity with IC50 value of 85.90±0.93µg/mL against Hep2c cells. SOD and GST activity was observed as highest in the APA(Mac) extract whereas the catalase activity and GSH content was maximum in the APE(Mac) extract. Further, the MDA content was observed to be the least in APE(Mac) extracts. Docking results suggested maximum binding energy between tannic acid and Her2 receptor compared to doxorubicin (standard). This study provides evidence that A. precatorius seed extracts possess promising bioactive compounds with probable anticancer and antioxidant properties against CaCx which might be utilized as a possible herbal remedy aimed at restricting tumor growth.

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

scholarly journals Comparative study of the antioxidant properties of silymarin and milk thistle extracts

2021 ◽  
Vol 19 (1) ◽  
pp. 1-6
Author(s):  
V. Ivanov ◽  
M. Pavlova
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Total Phenolic ◽  
Milk Thistle ◽  
Flavonoid Content ◽  
Silybum Marianum ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Purpose: Milk thistle (Silybum marianum (L.) Gaertn) has been used for centuries as a natural remedy for liver and gall bladder / biliary tract diseases. Silymarin is the purified and standardized extract from milk thistle seeds. The purpose of the present study is to present a comparative analysis of the total phenolic substances content and the antioxidant effect of silymarin and three different types of Silybum marianum extracts (i.e. ethanol extract, water extract and lyophilized extract). Methods: Total phenolic and flavonoid content of extracts was analysed and the antioxidant activity was assessed by determining their free radical scavenging ability by DPPH method. Results: Total phenolic content is the highest in silymarin– 134.12±3.22 mg GAE/g, followed by lyophilized water extract – 51.74±2.10 mg GAE/g. Silymarin has also been found to have the highest antioxidant activity among all extract samples (338.03 VCEAC µmol /g), followed by S.marianum lyophilised extract (123.05 VCEAC µmol/g). Conclusions: A strong correlation between total phenolic and flavonoid content in the extracts and their antioxidant activity has been found.

scholarly journals Influence of fertilizer and salicylic acid treatments on growth, physiological, and antioxidant characteristics in green and red Perilla frutescens varieties

2021 ◽  
Vol 49 (1) ◽  
pp. 12064
Author(s):  
Yin-Jheng JHOU ◽  
Kuan-Hung LIN ◽  
Chun-Wei WU ◽  
Yu-Sen CHANG
Keyword(s):  
Salicylic Acid ◽  
Vegetation Index ◽  
Agronomic Traits ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Fertilizer Treatment ◽  
Herbaceous Plant ◽  
Psii Photochemistry

Perilla is herbaceous plant, functional food, and nutraceutical product with antioxidant properties. The objective of this study was to investigate the growth, reflectance indices, and antioxidant properties of P. frutescens species in response to fertilizer and salicylic acid (SA) applications. Two independent experiments were carried out in an environmentally controlled greenhouse: (1) pots of red-leaf and green-leaf cultivars divided into four groups treated with 10-30-20 (10N-13.1P-16.6K), 15-10-30 (15N-4.4P-24.9K), 20-20-20 (20N-8.7P-16.6K), and 30-10-10 (30N-4.4P-8.3K) fertilizers for periods of 10 weeks, and (2) pots of red and green Perilla cultivars divided into five groups treated with 0 (control), 125, 250, 500, and 1,000 µM of SA for periods of 7 weeks. Wide variations occurred in the agronomic performance, soil-plant analysis development (SPAD) value, adjusted normalized difference vegetation index (NDVI), maximal quantum yield of PSII photochemistry (Fv/Fm), and antioxidant activity of the two Perilla varieties. All the measured traits were higher in green than in red Perilla under identical fertilizing, and all agronomic traits in green and red Perilla plants subjected to 125 and 500 μM SA were better than in controls. The SPAD and NDVI values of all plants increased as N% increased, the lowest Fv/Fm values of all plants were observed under 15-10-30 fertilizer treatment, the lowest NDVI values were detected in controls, and the Fv/Fm values of all plants decreased under 1,000 μM SA treatment. These indices can be used as indicators to characterize the physiology of these plants and are suitable for evaluating their growth and development under specific fertilizer and SA treatments. Green Perilla leaf extract (PLE) contained higher rosmarinic acid (RA) concentration in each fertilizer treatment, and higher total phenolic (TP) and RA concentration in each SA treatment. However, red PLE contained higher caffeic acid (CA) concentration than green PLE in each fertilizer and SA treatment, implying that their two genotypes exhibited different abilities and specificities of photosynthetic metabolites, and that different varieties may prepare for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity by up-regulating TP, RA, and CA concentration differently. Thus, Perilla plants can be used as health foods due to high TP, RA, and CA concentration. To produce Perilla efficiently in industrial applications, we undertook to determine the optimum N-P-K fertilizer ratio and SA application for maximizing the growth and accumulation of TP, RA, and CA in Perilla plants.

scholarly journals Micronized Oat Husk: Particle Size Distribution, Phenolic Acid Profile and Antioxidant Properties

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5443
Author(s):  
Dariusz Dziki ◽  
Wojciech Tarasiuk ◽  
Urszula Gawlik-Dziki
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Phenolic Acids ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffraction Method ◽  
Total Phenolic ◽  
Oat Husk

Oat husk (OH; hull) is a by-product generated from oat processing and is rich in insoluble fibre and phenolic compounds. The aim of this work was to study the particle size distribution, antioxidant activity, and phenolic profile of micronized OH. For this purpose, the hull was first sterilized using superheated steam and was then ground using an impact classifier mill. The particle size distribution (PSD) of the ground husk was determined using the laser diffraction method and the parameters characterizing the PSD of the ground husk, and its antioxidant activity were calculated. In addition, UPLC-MS/MS analysis of phenolic acids was also performed. Micronization of the sterilized husk effectively decreased the size of the particles, and with the increasing speed of the rotor and classifier, the median size of the particles (d50) decreased from 63.8 to 16.7 µm. The following phenolic acids were identified in OH: ferulic, caffeic, p-hydroxybenzoic, vanillic, syringic, and synapic acid. Ferulic acid constituted about 95% of total phenolic acids. The antioxidant activity of the obtained extracts increased as the particle size of the micronized husk decreased. The highest half maximal inhibitory concentration (EC50 index) was found for chelating power, and the lowest was found in the case of radical scavenging activity against DPPH.

scholarly journals Antioxidant and free radical scavenging effects of Iraqi sumac ( Rhus coriaria L)

2013 ◽  
Vol 10 (3) ◽  
pp. 921-933 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antioxidant Activity ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Rhus Coriaria ◽  
Dpph Radical Scavenging ◽  
Seed Extracts

This study was carried out to evaluate the antioxidant activity of Iraqi sumac seeds (Rhus coriaria. L) (Anacardiaceae). Total phenolic compounds and flavoniods were determined in three different sumac seed extracts (SSE) (aqueous,ethanolic and methanolic extract). For extraction Antioxidant activity of SSE were evaluated by various antioxidant assays, including total antioxidant capacity, reducing power,by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, nitric oxide scavenging, Hydroxyl radical scavenging, and metal ion chelating activities. These various antioxidant activities were compared with ascorbic acid as a standard antioxidant.The results showed that the three(SSE), contained large amounts of phenolic and flavoniods compounds. The results also showed that the three( SSEs ) have antioxidant activities , the methanolic (SSEs) contain larger amounts of phenolic and flavoniod compounds and have a higher antioxidant activities than that of aqueous or ethanolic (SSE).These results indicated that Iraqi aqueous and alcoholic extracts of Iraqi sumac in especial methanolic) SSE( are good scavengers for reactive oxygen species(ROS) and are a potential source of natural antioxidant, that may be used in pharmaceutical or food industry .

Effects of Drying Methods on Antioxidant Properties and Phenolic Content in White Button Mushroom

2012 ◽  
Vol 8 (3) ◽  
Author(s):  
Hongfang Ji ◽  
Ailin Du ◽  
Lingwen Zhang ◽  
Shuang Li ◽  
Mingduo Yang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Vacuum Drying ◽  
Drying Methods ◽  
Button Mushroom ◽  
Microwave Vacuum Drying ◽  
White Button Mushroom

Abstract In order to explore the potential drying process, the effects of different drying methods (sun drying, hot-air drying, microwave-vacuum drying, and freeze drying) on the antioxidant properties and phenolic contents in white button mushroom fruit bodies were investigated. The Folin-Ciocalteu method was used to determine total phenolic content, while 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, iron-chelating ability, and hydroxyl radical scavenging activity assays were used to elucidate antioxidant activities. Results indicated that antioxidant properties in dried white button mushroom were affected by the drying methods to different extents, and phenolic contents showed significant differences (p<0.05) among different drying methods. Microwave-vacuum drying possessed the highest antioxidant activity in DPPH radical scavenging, reducing power and iron-chelating ability, respectively; and the content of phenolic compounds was 4.945 mg gallic acid equivalents (GAE) /g dry weight (DW). Sun drying was the worst in antioxidant activity and content of phenolic compounds was 1.964 mg GAE/g DW. Additionally, significantly positive correlation was found between the total phenolic content and antioxidant activity (p<0.01). As far as antioxidant activities are concerned, microwave-vacuum drying is proposed as an appropriate drying technique for white button mushroom.

scholarly journals ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC AND FLAVONOID CONTENT VARIATIONS OF LEAF EXTRACTS OF LAURUS NOBILIS L. FROM MOROCCO

2018 ◽  
Vol 11 (12) ◽  
pp. 540 ◽  
Author(s):  
Amal Taroq ◽  
Fatima El Kamari ◽  
Imane Aouam ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Laurus Nobilis ◽  
Dry Extract ◽  
Water Extracts ◽  
Phenolic And Flavonoid

Objective: The present study was undertaken to determine antioxidant activity and total phenolic and flavonoid content of ethanol, methanol, ethyl acetate, and aqueous extracts of the leaves of Laurus nobilis L.Methods: Antioxidant properties were measured using three tests: Free radical scavenging activity against 2,2-diphenyl picrylhydrazyl (DPPH), reduction of molybdate, and reducing (Fe3+/Fe2+) power. Total phenolic and flavonoid content was measured by Folin–Ciocalteu and rutin reagent, respectively.Results: The results showed that both the ethanol and water extracts had greater antioxidant activity than ethyl acetate. Aqueous extract exhibited a higher DPPH radical scavenging and reducing molybdate 545.83±5.89 mg equivalent to ascorbic acid/g dry extract. The strong antioxidant activity of water extract was probably due to its high content of phenols. Furthermore, ethanol and water extracts showed higher total flavonoid content with the values: 153.33±3.59 and 127.25±2.60 mg equivalent rutin/g dry extract, respectively.Conclusion: Our results suggested a potent and excellent antioxidant activity of L. nobilis L. extracts of Morocco. Further research has to be done to isolate the pure bioactive compound and could be considered as a potential source of biomolecules for pharmaceutical and food industry.

scholarly journals Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts

2012 ◽  
Vol 66 (4) ◽  
pp. 547-552 ◽  
Author(s):  
Marija Radojkovic ◽  
Zoran Zekovic ◽  
Senka Vidovic ◽  
Drago Kocar ◽  
Pavle Maskovic
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Phenolic Components ◽  
Dpph Method ◽  
Morus Spp ◽  
Phenolic And Flavonoid

Mulberry (Morus spp. L., Moraceae) fruits, leaves, bark and branch have been used in traditional medicine as diuretic, hypoglycemic and hypotensive. The mechanism of their effects is correlated with the content of active components. Objective of this work was to evaluate and compare antioxidant properties of different extracts of two Morus species growing in Serbia: Morus alba L. (white mulberry) and Morus nigra L. (black mulberry). Potential antioxidant activity, content of antioxidant compounds (phenolics and flavonoids) and radical scavenging capacity, tested by DPPH method, were evaluated. The phenolic and flavonoid composition of different Morus extracts was determined by the HPLC method. The extracts prepared from fruits, leaves and roots of M. alba and M. nigra exhibited different characteristics. The highest extraction yield was achieved by M. alba leaves extraction (23.40%). M. nigra roots extract shown the highest total phenolics (186.30 mg CAE/g), while highest total flavonoids content (67.37 mg RE/g) was determined for M. nigra leaves extracts. In addition, black mulberry leaves extracts with the highest antioxidant activity had the highest phenolic acids contents. The dominant phenolic components in the samples were rutin and chlorogenic acid. All investigated mulberry dry extracts shown high content of phenolic compounds and significant antioxidant activity. This work contributes to knowledge of the antioxidant properties of Morus species. The obtained results may be useful in the evaluation of new dietary supplements and food products.

Phenolic contents and antioxidant activity of Helicteres hirsuta extracts

2020 ◽  
Vol 17 ◽  
Author(s):  
Le Trung Hieu ◽  
Tran Thi Van Thi ◽  
Le Lam Son ◽  
Nguyen Minh Nhung ◽  
Huynh Thi Ngoc Diep ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Strongly Correlated ◽  
Phenolic Fraction ◽  
Phenolic Contents ◽  
Plant Parts ◽  
Total Antioxidant

Abstract: Helicteres hirsuta, which belongs to the Malvaceae family, is used in traditional medicine to treat malaria, diabetes and cervical cancer. While these uses have not been validated in clinical studies thus far, extracts from H. hirsuta exhibit confirmed antioxidant and potential anti-cancer activity that warrant critical assessment. The aim of this study was to determine the antioxidant properties of different parts of the plant H. hirsuta. The antioxidant capacities of methanol extracts of different plant parts, and specific solvent fractions thereof were determined on the basis of 2,2-diphenyl1picrylhydrazyl (DPPH) radical scavenging and total antioxidant activity assays. The ethyl acetate fraction from leaf material of H. hirsuta has the most potent antioxidant activity with the lowest half maximal inhibitory con-centration (IC50) value of 9.50 μg/mL, that is 4 times lower than the IC50 of curcumin. The amounts of methyl gal-late and rutin dominated the extracts at 8.62 ± 0.01 and 6.76 ± 0.02 mg/g, respectively, out of a total of 21.00 ± 0.01 mg/g active phenolic fraction. The amount of methyl gallate strongly correlated with total phenolic content and total antioxidant capacity, thus this compound may be useful as a marker of antioxidant activity in common medicinal plants

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