scholarly journals Assessment of the In Vivo Release and Biocompatibility of Novel Vesicles Containing Zinc in Rats

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4101
Author(s):  
Liliana Mititelu-Tartau ◽  
Maria Bogdan ◽  
Daniela Angelica Pricop ◽  
Beatrice Rozalina Buca ◽  
Ana-Maria Pauna ◽  
...  
Keyword(s):  
Body Weight ◽  
Zinc Chloride ◽  
Histopathological Examination ◽  
Chitosan Solution ◽  
Lipid Vesicles ◽  
Biochemical Tests ◽  
Phagocytic Capacity ◽  
In Vivo Release ◽  
Group I

This paper is focused on the in vivo release and biocompatibility evaluation in rats of some novel systems entrapping zinc chloride in lipid vesicles. The particles were prepared by zinc chloride immobilization inside lipid vesicles made using phosphatidylcholine, stabilized with 0.5% chitosan solution, and dialyzed for 10 h to achieve a neutral pH. The submicrometric systems were physico-chemically characterized. White Wistar rats, assigned into four groups of six animals each, were treated orally with a single dose, as follows: Group I (control): deionized water 0.3 mL/100 g body weight; Group II (Zn): 2 mg/kg body weight (kbw) zinc chloride; Group III (LV-Zn): 2 mg/kbw zinc chloride in vesicles; Group IV (LVC-Zn): 2 mg/kbw zinc chloride in vesicles stabilized with chitosan. Haematological, biochemical, and immune parameters were assessed after 24 h and 7 days, and then liver fragments were collected for histopathological examination. The use of zinc submicrometric particles—especially those stabilized with chitosan—showed a delayed zinc release in rats. No substantial changes to blood parameters, plasma biochemical tests, serum complement activity, or peripheral neutrophils phagocytic capacity were noted; moreover, the tested substances did not induce liver architectural disturbances. The obtained systems provided a delayed release of zinc, and showed good biocompatibility in rats.

scholarly journals Monitoring of BALB/C strain mice health, investigation of behavior, hematological parameters under the effect of an electromagnetic field

2019 ◽  
Vol 75 (03) ◽  
pp. 6194-2019
Author(s):  
JUDITA ZYMANTIENE ◽  
RASA ZELVYTE ◽  
VIDA JUOZAITIENE ◽  
VAIDAS OBERAUSKAS ◽  
ALGIS NOREIKA ◽  
...  
Keyword(s):  
Body Weight ◽  
Mobile Phone ◽  
Mobile Phones ◽  
Electromagnetic Waves ◽  
Histopathological Examination ◽  
Hematological Parameters ◽  
Behavior Characteristics ◽  
Brain Behavior ◽  
The Brain

The aim of this study was to investigate the effect of electromagnetic fields on BALB/c strain mice on their health, body weight, behavior characteristics, hematological parameters and histopathological findings in the brain. The mice of the experimental groups were exposed to electromagnetic waves by using Nokia 230 and Samsung 19300 Galaxy S III mobile phones situated at 2 cm from the cages. In the present study, it can be concluded that the exposure of mice to mobile phone radiation had an effect on the structure of the brain, behavior and body weight. The waves of mobile phones increased activity characteristics and changed some behavioral categories of mice and also decreased their body weight. Histopathological examination revealed mild edema of neutrophils and degeneration of some neurons and glial cells in the brains of experimental mice. The results of the present study showed that a using mobile phone had an influence on in vivo systems.

scholarly journals ​Therapeutic Efficacy of Ethanolic Extract of Curcuma longa and its Component, Curcumin against Experimental Cryptosporidiosis in Mice

2021 ◽  
Author(s):  
Alveena Ganai ◽  
Anish Yadav ◽  
Rajesh Katoch ◽  
Dibyendu Chakraborty ◽  
Pawan Kumar Verma ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Curcuma Longa ◽  
Ethanolic Extract ◽  
Control Group ◽  
Rrna Gene ◽  
Histopathological Changes ◽  
Group I

Background: Cryptosporidiosis caused by Cryptosporidium spp. is a zoonotic disease and is the most prevalent pathogens worldwide and leads to severe diarrhoeal diseases and affects the immunological status of the individual. Thus, the study was undertaken to examine the anti-cryptosporidial efficacy of curcumin in comparison with ethanolic extract of curcuma longa in immunocompromised mice infected with oocysts isolated from cattle calves of Jammu region and identified as Cryptosporidium parvum using nested PCR on small subunit ribosomal ribonucleic acid (SSU rRNA) gene. Methods: Two hundred female Swiss albino mice were equally divided into ten groups. Group I were kept as a healthy control, group II were immunocompromised, group III were immunocompromised and infected, group IV animals were immunocompromised, infected and treated orally with nitazoxanide. Animals in groups V to VII were immunocompromised, infected and treated with ethanolic extract of C. longa @ 4, 6 and 8 mg/kg/day/os respectively whereas groups VIII to X were immunocompromised, infected and treated with pure salt of curcumin @ 4, 6 and 8 mg/kg/day/os respectively for 5 successive days. Thus, mean oocysts per gram faeces, body weight gain and histopathological changes were measured in different groups. Result: Administration of curcumin as a therapeutic agent @ 8 mg/kg body weight for five days resulted in higher percent mean oocyst reduction of 74.03% and improved body weight gain in experimentally infected mice. Histopathological changes showed that treatment with oral curcumin (group X) in animals had minimal and improved intestinal lesions as compared to animals treated with C. longa (group VII). Altogether, curcumin showed promising anticryptosporidial effects under in vivo conditions and deserves further exploration.

In vivo curative antimalarial effects of the aqueous extract of Entandrophragma angolense bark on Plasmodium berghei infection in mice

2019 ◽  
Author(s):  
Raceline Gounoue Kamkumo ◽  
Abel Narcisse Messi Betene ◽  
Patrick Valère Tsouh Fokou ◽  
Jean Hubert Donfack ◽  
Marius Jaures Tsakem Nangap ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Malaria Control ◽  
Plasmodium Berghei ◽  
Venous Blood ◽  
Parasite Development ◽  
Distilled Water ◽  
Biochemical Tests

Abstract Background Research for new antimalarial drugs remains a permanent quest for the control of malaria disease due to the emerging parasite resistances. The present study investigates the effects of the aqueous extract of Entandrophragma angolense (E. angolense) bark on Plasmodium. berghei-induced malaria in mice. Methods Eight weeks old female mice, were intraperitoneally infested with 200 μL of mouse blood, containing 1x106 P. berghei-infected-erythrocytes. Parasitaemia was determined using a 10% giemsa stained blood smear read under optical microscope (x100). The infected animals were randomized into 5 groups of 10 animals each and daily treated for 5 days with the plant extract at 125, 250 and 500 mg / kg. The malaria control received distilled water (10 mL / kg) while the chloroquine control was treated with 10 mg / kg of chloroquine. A group of healthy mice was used as the normal control and received distilled water. Body weight, parasitaemia and survival time were monitored daily during treatment and follow up periods. Five animals from each group were sacrificed under anaesthesia at the end of treatment (d8) and after the follow up period (d28). Venous blood was used for haematological and biochemical tests. Organs (liver, kidneys and spleen) were also collected for biochemical and histological analyses. Results Administration of the aqueous extract of E. angolense bark to infected mice significantly inhibited parasite development (p < 0.001) with ED50 estimated at 25.32 mg / kg. The extract prevented animal from death, body weight loss, anaemia, leucocytosis, high transaminases (ALT and AST), high bilirubin, creatinine and MDA levels, oxidative stress and anatomical alteration in organs as compared to the malaria control. Conclusions The E. angolense bark possesses antimalarial properties, supporting its use in traditional medicine to treat malaria.

scholarly journals Pengembangan Produk Minuman Tonikum Kulit Kayu Akway (Drymis piperita): Prospek Penambah Stamina Tubuh Secara In Vivo

2021 ◽  
Vol 13 (1) ◽  
pp. 44-51
Author(s):  
Noviana Hallik ◽  
Rani D. Pratiwi ◽  
Elsye Gunawan
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Total Period ◽  
Physical Quality ◽  
Group Iii ◽  
Group I ◽  
The One ◽  
Base Group

Akway bark (Drymis piperita) from Papua is widely used by local people in Arfak Mountains of West Papua to increase stamina. The purpose of this study was to determine the physical quality and tonic effects of Akway bark (bark tonic drink D. piperita) on mice (Mus musculus). The swimming resistance used to determine the tonic effect of mice (24 individuals) which divided into 6 groups. Group I was given water, group II tonic drink base, group III as positive control of 50 mg caffeine energy drinks, groups IV, V, and VI were given bark tonic drink 10mg /kg body weight, 30 mg/kg body weight, and 50mg /kg body weight respectively. The total period swimming until fatigue was measured and used as an index of swimming capacity. The results of  physical quality of the Akway bark tonic drink has a sweet and sour taste, with clear dark brown color, had distinctive aroma with pH of 4, and its volume was 100 ml with solubility deposits on day 21. The results of pre-clinical testing shown the highest dose was 50 mg/ weight swimming time 9.28 minutes and having better stamina. The next was with dose of 30 mg/kgBW, swimming for for 6.01 minutes and the last was dose of 10 mg /kgBW swimming for 3.23 minutes. The results of the one-way ANOVA test showed that it was significantly different between the 10 mg/kgBW, 30 mg/kgBW and 50 mg/kgBW groups. Key words: drink; D. piperita tonic; effect; mice; Papua. 

scholarly journals Genauigkeit der Dualenergie-Röntgenabsorptiometrie (DXA) zur Ermittlung der Körperzusammensetzung von Schweinen <i>in vivo</i>

2006 ◽  
Vol 49 (5) ◽  
pp. 462-476 ◽  
Author(s):  
A. M. Scholz ◽  
M. Förster
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Bone Mineral ◽  
The Body ◽  
Whole Body ◽  
Lean Meat ◽  
Group I ◽  
Group Ii ◽  
Weight Groups

Abstract. Title of the paper: Accuracy of dual energy X-ray absorptiometry (DXA) for the determination of the body composition of pigs in vivo New methods for the measurement of the body composition in vivo (or post mortem) like DXA need to be evaluated before routine use in livestock research or performance testing. Two body weight groups of pigs I) 30–50 kg (n=61) and II) 70–90 kg (n=62) were studied in this analysis. The whole bodies of the pigs were analyzed by a GE Lunar DPX-IQ scanner in vivo for the amount and percentage of fat, lean tissue, bone mineral, and the bone mineral density as well. Three days later, pigs were slaughtered and after a 24 h cooling period, the reference parts (shoulder, loin, side, and ham) of the left carcass half were dissected into lean meat, fat, bone, tendons, connective tissue and skin according to the EU reference method. Generally, DXA variables explained a high proportion of variation (R²>0.8; √MSE=70–784 g) in the tissue masses of the reference parts (fat, muscle, bone) in both weight groups. The fat percentage of the reference parts and the DXA whole body results (in vivo) showed a very high relationship with a maximum R²=0.82 (√MSE=1.47%) for group II. Slightly lower was the relationship between the lean meat content (%) of the reference parts and DXA results in vivo (R2=0.72; √MSE=1.75) for group II. The bone percentage was predicted with R²=0.3 (√MSE=0.64), caused by the very different analysis methods. Generally, DXA is a suitable method for growth studies in the above body weight range. In order to predict the percentage of muscle (lean meat) or fat tissue accurately by DXA, pigs should weigh >50 kg, since the soft tissue percentages of group I resulted in R²≤0.38 (√MSE≤2.55).

Evaluation of antidiabetic effect of Cistus salviifolius L. (Cistaceae) in streptozotocin-nicotinamide induced diabetic mice

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Karima Sayah ◽  
Hanae Naceiri Mrabti ◽  
Badia Belarj ◽  
Faouzi Kichou ◽  
Yahia Cherrah ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Histopathological Examination ◽  
Diabetic Control ◽  
Functional Markers ◽  
Histopathological Analysis ◽  
Diabetic Mice ◽  
Standard Drug ◽  
Cistus Salviifolius

AbstractObjectivesCistus salviifoluis L. is a shrub from Cistaceae family used in many traditional medicines for the treatment of various diseases including diabetes mellitus. The aim of this study was to evaluate the in vivo antidiabetic potential of the aerial parts aqueous extract of Cistus salviifolius L. (CSA).MethodsExperimental diabetes was induced in adult male mice by intra-peritoneal injection of Streptozotocin-nicotinamide (STZ-NC). CSA at a dose of 500 mg/kg was administered daily to the diabetic mice for four weeks. The effect of the extract on hyperglycemia, body weight, serum total cholesterol, triglycerides, hepatic and renal functional markers were determined. Histopathological examination of the mice pancreas was also performed. The diabetic animals treated with CSA were compared with animals treated by the standard drug metformin.ResultsTreatment with CSA showed a significant reduction in blood glucose, total triglycerides and creatinine levels and prevented the reduction of body weight caused by diabetes. Furthermore, histopathological analysis of the mice pancreas showed that the group treated with CSA reduced damage induced by STZ-NC on islets of Langerhans cells when compared to the diabetic control.ConclusionsThe results suggest that the aqueous extract of Moroccan C. salviifolius L. possesses beneficial effect on treatment of diabetes.

scholarly journals IN VIVO INVESTIGATION OF BOVINE CUTANEOUS PAPILLOMATOSIS WITH HOMEOPATHIC PREPARATIONS

2012 ◽  
Vol 3 (2) ◽  
pp. 114-116
Author(s):  
MG Hossain ◽  
MG Haider ◽  
MR Khan ◽  
EH Chowdhury ◽  
MM Hossain
Keyword(s):  
Untreated Control ◽  
Histopathological Examination ◽  
Control Group ◽  
Group Iii ◽  
Concurrent Use ◽  
Group I ◽  
Cellular Necrosis ◽  
Group Ii ◽  
Control Group Group

This investigation was carried out to determine the effects of thuza and antim crud (Homeopathic preparations) on bovine cutaneous papillomatosis/wart. Nine calves of one to two and a half years age and of both sexes affected with cutaneous papillomatosis were randomly divided into three equal groups (group I, group II and group III) for this experiment from March 2002 to February 2003. Each calf of group I was first administered with 15 globules sulphur with a potency of 200-power once orally. This was followed after 7 days with 50M (M = 1000-power) thuza at the dose rate of 10 globules twice daily orally for a period of three weeks. The calves of group II were administered with sulphur and antim crud at the same dose, potency, route and duration, respectively and the calves of group III were kept as untreated control. Papilloma tissues were collected prior to treatment and at the sixth week of treatment and they were subjected to histopathological examination. Thuza and antim crud with concurrent use of sulphur were found effective in curing bovine cutaneous papillomatosis with a rate of 66.66% (4/6). Little or no reduction in size of the papillomas was observed within the first two weeks of treatment in both the treated groups (group I and group II). Thereafter, miniature size sessile and pedunculated papillomas started drying with the gradual reduction in size that fell leaving hairless areas at the end of fourth week. By the eighth week there was complete cure of papillomas. One calf of each treated groups (group I and group II) affected with large papillomas (5 X 7 cm) were not completely cured. Microscopic examination of regressive papillomas at sixth week of treatment showed cellular necrosis. Growth recurrence was not observed within one month of recovery. In the untreated control group (group III), the number and size of papillomas increased. Therefore, this line of treatment may be practiced for the treatment of bovine papillomatosis.

scholarly journals In vitro and in vivo evaluation of silver needle white tea extract against colon cancer

2020 ◽  
Author(s):  
Fatemeh Hajiaghaalipour ◽  
Elham Bagheri ◽  
Elham Rouhollahi ◽  
Nur'ain Salehen ◽  
Mahmood Ameen Abdulla
Keyword(s):  
Colon Cancer ◽  
Body Weight ◽  
Acute Toxicity ◽  
Aberrant Crypt Foci ◽  
Male Rats ◽  
White Tea ◽  
Group I ◽  
Tea Extract

Abstract Background Tea (Camellia sinensis) is one of the most consumed beverages in the world. White tea is an unfermented tea made from young tea leaves or unopened buds. Methods The in vitro antiproliferation assay and in vivo Chemopreventive effects of white tea extract (WTE) using azoxymethane (AOM) induced aberrant crypt foci (ACF) in rats was assessed. Twenty-four adult male rats were injected 15 mg/kg body weight AOM subcutaneously once a week for two weeks and then were divided randomly into four groups. Group I (Cancer control), Group II (treated whit 200 mg/kg white tea extract), Group III (treated whit 400 mg/kg white tea extract) and group IV (35 mg/kg body weight 5-fluorouracil as reference control). On the last day of the experiment, the animals were euthanized using an overdose of ketamine and xylazine and the colon tissue was collected. The colon tissues were evaluated grossly and histopathologically for aberrant crypt foci (ACF). Prior to the cancer studies, the acute toxicity test was performed to demonstrate the safety usage of the extract. Results The antiproliferative activity of the extract was observed in HT-29 and HCT 116 colon cancer cells. The ACF score was significantly reduced following the white tea extract treatment in a dose-dependent manner. Furthermore, the acute toxicity study indicated that there were no nephrotoxic and hepatotoxic effects or any serum biochemical changes in the rats that were orally administered with 2000 mg/kg body weight of the extract. Conclusions This study suggested the the potential of white tea as a chemopreventative agent through inhibition of Azoxymethane-induced Colonic Aberrant Crypt Foci Formation in the male Sprague-Dawley rats.

In vivo assessment of bisphenol A induced histopathological alterations and inflammatory gene expression in lungs of male Wistar rats

2020 ◽  
pp. 096032712095845
Author(s):  
A Rehman ◽  
T Akhtar ◽  
N Hameed ◽  
N Sheikh
Keyword(s):  
Body Weight ◽  
Bisphenol A ◽  
Lung Damage ◽  
Matrix Metalloproteinase 2 ◽  
Control Group ◽  
Limited Information ◽  
Altered Expression ◽  
Group I ◽  
Male Wistar Rats

Bisphenol A (BPA), an imperative environmental contaminant used in polycarbonate plastics. Due to limited information concerning the effect of BPA on lungs, this study design to assess whether BPA cause alterations in histopathology and trace metal content in lungs of rats. They were divided into five groups with five rats per group. Group I was named as control group. Group L6 and L12 were received BPA (10 mg/kg body weight/day) for 6 weeks and 12 weeks respectively. Group H6 and L12 were given BPA (25 mg/kg body weight/day) for 6 weeks and 12 weeks respectively. Considerable alteration in Cu, Zn and Fe was detected in experimental groups. BPA also caused significant increase in the expression of tumor necrosis factor α that mediate the pulmonary inflammatory response. Comparative study of resolved proteins i.e. 72 KDa (matrix metalloproteinase 2 fragment) and 109 KDa (nucleolin) on SDS-PAGE showed their altered expression in experimental groups. Histopathology of experimental groups revealed altered architecture of lungs. Special staining of BPA treated groups showed significant number of mast cells in alveoli and bronchioles. Prolonged administration of BPA causes deleterious aggravating lung damage even at extremely low dose, so the use of BPA should be prohibited in plastic synthesizing industries.

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