scholarly journals Antimicrobial Activity of Gemini Surfactants with Ether Group in the Spacer Part

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5759
Author(s):  
Bogumil Eugene Brycki ◽  
Adrianna Szulc ◽  
Iwona Kowalczyk ◽  
Anna Koziróg ◽  
Ewelina Sobolewska
Keyword(s):  
Antimicrobial Activity ◽  
Gemini Surfactants ◽  
Alkyl Chain ◽  
Antimicrobial Properties ◽  
Structure Modification ◽  
Ether Group ◽  
Minimal Inhibitory Concentrations ◽  
Minimal Concentration ◽  
The Relationship ◽  
And Staphylococcus Aureus

Due to their large possibility of the structure modification, alkylammonium gemini surfactants are a rapidly growing class of compounds. They exhibit significant surface, aggregation and antimicrobial properties. Due to the fact that, in order to achieve the desired utility effect, the minimal concentration of compounds are used, they are in line with the principle of greenolution (green evolution) in chemistry. In this study, we present innovative synthesis of the homologous series of gemini surfactants modified at the spacer by the ether group, i.e., 3-oxa-1,5-pentane-bis(N-alkyl-N,N-dimethylammonium bromides). The critical micelle concentrations were determined. The minimal inhibitory concentrations of the synthesized compounds were determined against bacteria Escherichia coli ATCC 10536 and Staphylococcus aureus ATCC 6538; yeast Candida albicans ATCC 10231; and molds Aspergillus niger ATCC 16401 and Penicillium chrysogenum ATCC 60739. We also investigated the relationship between antimicrobial activity and alkyl chain length or the nature of the spacer. The obtained results indicate that the synthesized compounds are effective microbicides with a broad spectrum of biocidal activity.

scholarly journals Antimicrobial Activity of Gemini Surfactants with Azapolymethylene Spacer

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4054 ◽  
Author(s):  
Iwona Kowalczyk ◽  
Marta Pakiet ◽  
Adrianna Szulc ◽  
Anna Koziróg
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Penicillium Chrysogenum ◽  
Gemini Surfactants ◽  
1H And 13C Nmr ◽  
Minimal Inhibitory Concentrations ◽  
Reaction Proceeds ◽  
The Relationship

A series of 21 azapolymethylene gemini surfactants were obtained. The synthesis of the title surfactants in one- or two-step reaction proceeds with good yields. The structure and the purity of the synthesized compounds were determined by 1H and 13C NMR, ESI-MS spectra, and elemental analysis. Moreover, 2D COSY, HMBC, and HSQC spectra were performed. The minimal inhibitory concentrations (MIC) of the synthesized compounds were determined against fungi: Candida albicans, Aspergillus niger, Penicillium chrysogenum and bacteria: Escherichia coli,Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis. Also, the critical micelle concentrations (CMC) were determined. The relationship between antimicrobial and surface activity and surfactant structure has been determined.

scholarly journals Antimicrobial activities of laboratory produced essential oil solutions against five selected fungal strains

2013 ◽  
pp. 171-183 ◽  
Author(s):  
Emilija Ivanova ◽  
Natalija Atanasova-Pancevska ◽  
Dzoko Kungulovski
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Microtiter Plate ◽  
Antimicrobial Activities ◽  
Food Preservation ◽  
Minimal Inhibitory Concentrations ◽  
Fungal Strains ◽  
Treat Ment ◽  
Penicillium Spp

It is well known that essential oils possess significant antimicrobial activity. This study was conducted to estimate the antimicrobial activity of various types of Biokill, a laboratory produced solution composed of several essential oils (Biokill dissolved in 96% ethanol; Biokill 96% further dissolved in DMSO; Biokill dissolved in 70% ethanol and Biokill 70% further dissolved in DMSO). The antimicrobial activity was evaluated against five selected fungal strains, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger I.N. 1110, Aspergillus sojae CCF and Penicillium spp. FNS FCC 266. A variation of the microtiter plate-based antimicrobial assay was used in order to assess the antimicrobial activity of the solutions. By applying this assay minimal inhibitory concentrations (MIC) of the Biokill solutions were determined for each strain of the selected test microorganisms. The results demonstrated that all variations of Biokill showed antimicrobial activity at concentrations lower than 2.5?g/mL. Biokill 70% further dissolved in DMSO showed the best antimicrobial properties against all the selected strains with MICs less than 1.25?g/mL. These results indicated that Biokill could find application in the pharmaceutical industry, in food preservation and conservation, in the prevention and treat?ment of plants infected by certain phytopathogens, etc.

scholarly journals DESIGN, SYNTHESIS, AND PHARMACOLOGICAL EVALUATION OF SOME NOVEL BIS-THIAZOLE DERIVATIVES

2018 ◽  
Vol 11 (4) ◽  
pp. 164 ◽  
Author(s):  
Ramesh M Borde ◽  
Satish B Jadhav ◽  
Rahul R Dhavse ◽  
Achut S Munde
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Hantzsch Reaction ◽  
Thiazole Derivatives ◽  
Design Synthesis ◽  
Structure Activity ◽  
Elemental Analyses ◽  
And Staphylococcus Aureus ◽  
Toxic Molecule

 Objective: A series of substituted 5,2-bis-thiazoles derivatives were synthesized by Hantzsch reaction and evaluated in vitro for antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus. Methods: 2-(4-(benzyloxy)phenyl)-4-methylthiazole-5-carbothioamide were synthesized and allowed to react with various α-haloketones to give 5,2-bis-thiazoles, i.e., 2-(4-(benzyloxy)phenyl)-4-methyl-5-(4-substituted thiazol-2-yl)thiazole derivatives in excellent yield. The synthesized compounds were characterized by spectroscopic methods as well as elemental analyses. They were screened for their antimicrobial activity using the agar diffusion method.Result: Literature survey reveals that the synthesis of 2-(4-(benzyloxy)phenyl)-4-methyl-5-(4-substituted thiazol-2-yl)thiazole, i.e., (5,2-Bis-thiazoles) derivatives (10a-e) was not reported. The entire compound exhibited mild to moderate antimicrobial activity.Conclusion: The antimicrobial results revealed that the synthesized derivatives have significant antimicrobial properties, and further, structure– activity relationship studies may develop more potent and less toxic molecule.

scholarly journals FUNCTIONAL EFFECTS OF θ-DEFENSINS FROM BLOOD LEUKOCYTES OF BABOON PAPIO HAMADRYAS

2018 ◽  
pp. 66-73
Author(s):  
E. V. Tsvetkova ◽  
G. M. Aleshina ◽  
L. E. Leonovа ◽  
O. V. Shamova ◽  
E. V. Romanovskaya ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ionic Strength ◽  
Membrane Permeability ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
High Ionic Strength ◽  
Papio Hamadryas ◽  
Blood Leukocytes ◽  
Minimal Inhibitory Concentrations

Aim. Study the functional properties of cationic antimicrobial peptides 0-defensins isolated from baboon Papio hamadryas blood leukocytes. Materials and methods. Baboon 0-defensins were extracted from leukocyte mass using ultrafiltration, preparative electrophoresis and reverse phase high performance liquid chromatography. The test microorganisms used were Gram-negative bacteria Escherichia coli, Gram-positive bacteria Listeria monocytogenes and Staphylococcus aureus, and fungi Candida albicans. Minimal inhibitory concentrations (MICs) were determined by testing serial dilutions of the test peptides by radial diffusion in agarose gel. Microbicidal action was evaluated by counting surviving colony forming units after incubation microorganisms with the peptides. The 0-defensins influence on E.coli membrane permeability was assessed using chromogenic markers o-nirofenil- β-D-galactopyranoside and nitrocefin. Results. The analysis of the 0-defensins antimicrobial properties showed that they produce antimicrobial activity against test microorganisms, exhibiting bactericidal and fungicidal effects at micromolar concentrations. We studied the influence of different environmental conditions (low and high ionic strength, blood serum in medium) for antimicrobial activity. It is shown that 0-defensins have the ability to increase the outer membran of E.coli permeability, however, in contrast to 0-defensins have no noticeable influence on the inner membrane permeability. Conclusion. Baboon 0-defensins isolated from blood leukocytes are effective antimicrobial agents with a broad spectrum of microbicidal action. 0-Defensins baboon unlike 0-defensins exhibit antimicrobial activity in environments with high ionic strength. 0-Defensins increase the outer membran of E.coli permeability but not the cytoplasmic membrane, suggesting that the mechanism of antimicrobial effect of these peptides other than 0-defensins.

scholarly journals Antibiofilm and Antimicrobial Activity of Polyethylenimine: An Interesting Compound for Endodontic Treatment

2015 ◽  
Vol 16 (6) ◽  
pp. 427-432 ◽  
Author(s):  
Irene Pina-Vaz ◽  
Joana Barros ◽  
Ana Dias ◽  
Miguel A Rodrigues ◽  
Cidália Pina-Vaz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Flow Cytometric Analysis ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Endodontic Treatment ◽  
Antibiofilm Activity ◽  
Minimal Inhibitory Concentrations ◽  
Fluorescent Markers ◽  
Interesting Compound ◽  
Treatment Procedures

ABSTRACT Aim Bacteria levels of necrotic teeth are greatly reduced after endodontic treatment procedures but the presence of persisting microorganisms leads to continuous efforts to develop materials with antimicrobial properties. The purpose of the study was to determine the antimicrobial activity of polyethylenimine (PEI) against common bacteria and yeasts, regarding planktonic cells and biofilm, and to clarify its antimicrobial mechanism of action through flow cytometry. Materials and methods The antibiofilm and antimicrobial effect of PEI was determined against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Candida albicans strains using reference protocols. The effect of PEI was evaluated regarding adhesion, biofilm formation and biofilm disaggregation. In order to understand PEI cellular effects flow cytometric analysis was performed with different fluorescent markers. Results It was verified that minimal inhibitory concentrations (MIC) values and minimal lethal concentrations (MLC) obtained for PEI were similar and ranged between 50 and 400 mg/l, proving the microbicidal and fungicidal activity of this compound. Antibiofilm activity was also proved for all the microorganisms. Severe lesion of the membrane and cell depolarization was demonstrated. Conclusion Polyethylenimine showed antimicrobial and antibiofilm activity against microorganisms often associated with apical periodontitis. Clinical significance Theoretically, prolonging the antibacterial effects of materials used in endodontics may be interesting to help prevent reinfection and possibly to affect residual bacteria that survived the treatment procedures. How to cite this article Barros J, Dias A, Rodrigues MA, Pina-Vaz C, Lopes MA, Pina-Vaz I. Antibiofilm and Antimicrobial Activity of Polyethylenimine: An Interesting Compound for Endodontic Treatment. J Contemp Dent Pract 2015;16(6):427-432.

scholarly journals Improvement of the Antimicrobial Activity of Oregano Oil by Encapsulation in Chitosan–Alginate Nanoparticles

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 7017
Author(s):  
Krassimira Yoncheva ◽  
Niko Benbassat ◽  
Maya M. Zaharieva ◽  
Lyudmila Dimitrova ◽  
Alexander Kroumov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Water Solubility ◽  
Food Additives ◽  
Antimicrobial Properties ◽  
Aqueous Dispersion ◽  
In Vitro Cytotoxicity ◽  
Application Site ◽  
Minimal Inhibitory Concentrations ◽  
Oregano Oil

Oregano oil (OrO) possesses well-pronounced antimicrobial properties but its application is limited due to low water solubility and possible instability. The aim of this study was to evaluate the possibility to incorporate OrO in an aqueous dispersion of chitosan–alginate nanoparticles and how this will affect its antimicrobial activity. The encapsulation of OrO was performed by emulsification and consequent electrostatic gelation of both polysaccharides. OrO-loaded nanoparticles (OrO-NP) have small size (320 nm) and negative charge (−25 mV). The data from FTIR spectroscopy and XRD analyses reveal successful encapsulation of the oil into the nanoparticles. The results of thermogravimetry suggest improved thermal stability of the encapsulated oil. The minimal inhibitory concentrations of OrO-NP determined on a panel of Gram-positive and Gram-negative pathogens (ISO 20776-1:2006) are 4–32-fold lower than those of OrO. OrO-NP inhibit the respiratory activity of the bacteria (MTT assay) to a lower extent than OrO; however, the minimal bactericidal concentrations still remain significantly lower. OrO-NP exhibit significantly lower in vitro cytotoxicity than pure OrO on the HaCaT cell line as determined by ISO 10993-5:2009. The irritation test (ISO 10993-10) shows no signs of irritation or edema on the application site. In conclusion, the nanodelivery system of oregano oil possesses strong antimicrobial activity and is promising for development of food additives.

scholarly journals Structure Modification of an Active Azo-Compound as a Route to New Antimicrobial Compounds

2017 ◽  
Author(s):  
Simona Concilio ◽  
Lucia Sessa ◽  
Anna Maria Petrone ◽  
Amalia Porta ◽  
Rosita Diana ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Hydroxyl Groups ◽  
Structure Modification ◽  
Minimal Inhibitory Concentrations ◽  
Vis Spectroscopy ◽  
1H Nuclear Magnetic Resonance ◽  
Protein Receptors ◽  
Plausible Mechanism ◽  
Bacteria And Fungi

Some novel (phenyl-diazenyl)phenols (3a–g) were designed and synthesized to be evaluated for their antimicrobial activity. A previously synthesized molecule, active against bacteria and fungi, was used as lead for modifications and optimization of the structure, by introduction/removal or displacement of hydroxyl groups on the azobenzene rings. The aim of this work was to evaluate the consequent changes of the antimicrobial activity and to validate the hypothesis that, for these compounds, a plausible mechanism could involve an interaction with protein receptors, rather than an interaction with membrane. All newly synthesized compounds were analyzed by 1H nuclear magnetic resonance (NMR), DSC thermal analysis and UV-Vis spectroscopy. The in vitro minimal inhibitory concentrations (MIC) of each compound was determined against Gram-positive and Gram-negative bacteria and Candida albicans. Compounds 3b and 3g showed the highest activity against S. aureus and C. albicans, with remarkable MIC values of 10 µg/mL and 3 µg/mL, respectively. Structure- activity relationship studies were capable to rationalize the effect of different substitutions on the phenyl ring of the azobenzene on antimicrobial activity.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

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