scholarly journals Metabolite Profiling of Helichrysum italicum Derived Food Supplements by 1H-NMR-Based Metabolomics

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6619
Author(s):  
Antonietta Cerulli ◽  
Milena Masullo ◽  
Sonia Piacente
Keyword(s):  
Phenolic Compounds ◽  
1H Nmr ◽  
Chemical Constituents ◽  
Antioxidant Properties ◽  
Mediterranean Area ◽  
Food Supplements ◽  
Bioactive Metabolites ◽  
Water Mixture ◽  
Multivariate Statistical ◽  
Dicaffeoylquinic Acid

Helichrysum italicum is a medicinal plant from the Mediterranean area, widely used in traditional medicine for its anti-inflammatory, antibacterial and antioxidant properties and for its preventive effects on microcirculation diseases. Due to these properties, it finds large applications in cosmetic, food and pharmaceutical fields. Additionally, hydroalcoholic extracts and mother tinctures based on H. italicum represent products with a high commercial value, widely distributed not only in drug stores but also on on-line markets. The different extraction procedures used can greatly affect the fingerprints of the extracts, resulting in a different qualitative or quantitative profile of the chemical constituents responsible for biological activity. The aim of the present study was to characterize the composition of bioactive compounds present in water-ethanol and glycerol extracts of H. italicum derived food supplements. Metabolite profiles of the extracts were obtained by 1H NMR experiments and data were processed by multivariate statistical analysis to highlight differences in the extracts and to evidence the extracts with the highest concentrations of bioactive metabolites. In detail, this work highlights how derived food supplements of H. italicum obtained using ethanol-water mixtures ranging from 45% to 20% of ethanol represent the products with the highest amount of both primary (amino acids) and secondary metabolites including 3,4-dicaffeoylquinic acid (9), chlorogenic acid (10), 3,5-dicaffeoylquinic acid (11), and kaempferol 3-O-glucopyranoside (12). Moreover, it is evident that the use of an ethanol-water mixture 20:80 is the most suitable method to afford the highest number of phenolic compounds, while food-derived supplements obtained by glycerol extraction are characterized by a high amount of β-glucose and α-glucose and a low content of phenolic compounds.

scholarly journals Phenolic profile and antioxidant properties of dried buckwheat leaf and flower extracts

2017 ◽  
Vol 23 (1) ◽  
pp. 39-47
Author(s):  
Aleksandra Misan ◽  
Bojana Saric ◽  
Ivan Milovanovic ◽  
Pavle Jovanov ◽  
Ivana Sedej ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Extraction Efficiency ◽  
Antioxidant Properties ◽  
Vascular Diseases ◽  
Cost Effective ◽  
Model Systems ◽  
Free Radical Scavengers ◽  
High Yield ◽  
Water Mixture ◽  
Phenolic Profile

Due to a high content of rutin (2-10%), dried buckwheat leaf and flower (DBLF) formulations were shown to be efficient in the treatment of vascular diseases. In order to find a cost effective way for the extraction of antioxidants, the effects of ethanol/water ratio and temperature on the extraction efficiency of phenolic compounds and the mechanisms of antioxidant action of the extracts were tested. Extraction with ethanol/water mixture (80:20, v/v) for 24 h at room temperature, after the mixture was just brought to boil was demonstrated to be an efficient and cheap way for obtaining a high yield of rutin (49.94?0.623 mg/g DBLF). The most abundant phenolic compounds in DBLF extracts were rutin and chlorogenic acid. Flavonoids, especially rutin, were shown to be the most responsible for the antioxidant activity in all investigated lipid model systems, acting as free radical scavengers, electron-donating substances and chelators of iron ions. In ?-carotene bleaching tests, the extracts with the highest activity were as efficient as BHT (butylated hydroxytoluole). Regarding the results of antihemolytic and Schaal oven tests, the extracts demonstrated remarkable ability to inhibit the oxidative destruction of erythrocytes and to prolong the beginning of the oxidation process in sunflower oil.

scholarly journals Hydrothermal Processing and In Vitro Simulated Human Digestion Affects the Bioaccessibility and Bioactivity of Phenolic Compounds in African Pumpkin (Momordica balsamina) Leaves

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5201
Author(s):  
Siphosanele Mafa Moyo ◽  
June C. Serem ◽  
Megan J. Bester ◽  
Vuyo Mavumengwana ◽  
Eugenie Kayitesi
Keyword(s):  
Oxidative Stress ◽  
Phenolic Compounds ◽  
Spinacia Oleracea ◽  
Hydrothermal Process ◽  
The Body ◽  
Bioactive Metabolites ◽  
Hydrothermal Processing ◽  
Caffeoylquinic Acid ◽  
Dicaffeoylquinic Acid

The African pumpkin (Momordica balsamina) contains bioactive phenolic compounds that may assist in reducing oxidative stress in the human body. The leaves are mainly consumed after boiling in water for a specific time; this hydrothermal process and conditions of the gastrointestinal tract may affect the presence and bioactivity of phenolics either positively or negatively. In this study, the effects of hydrothermal processing (boiling) and in vitro simulated human digestion on the phenolic composition, bioaccessibility and bioactivity in African pumpkin were investigated in comparison with those of spinach (Spinacia oleracea). A high-resolution ultra-performance liquid chromatography, coupled with diode array detection, quadrupole time-of-flight and mass spectrometer (UPLC-DAD-QTOF-MS) was used to profile phenolic metabolites. Metabolites such as 3-caffeoylquinic acid, 5-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid were highly concentrated in the boiled vegetable extracts compared to the raw undigested and all digested samples. The majority of African pumpkin and spinach extracts (non-digested and digested) protected Deoxyribonucleic acid (DNA), (mouse fibroblast) L929 and human epithelial colorectal adenocarcinoma (Caco-2) cells from 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative damage. From these results, the consumption of boiled African pumpkin leaves, as well as spinach, could be encouraged, as bioactive metabolites present may reduce oxidative stress in the body.

scholarly journals Changes in Bovine Colostrum Metabolites during Early Postpartum Period Revealed by 1H-NMR Metabolomics Approach

2021 ◽  
Vol 44 (2) ◽  
pp. 229-239
Author(s):  
S. Settachaimongkon ◽  
N. Wannakajeepiboon ◽  
P. Arunpunporn ◽  
W. Mekboonsonglarp ◽  
D. Makarapong
Keyword(s):  
1H Nmr ◽  
Chemical Constituents ◽  
Bovine Colostrum ◽  
Proton Nuclear Magnetic Resonance ◽  
Bioactive Metabolites ◽  
Nmr Metabolomics ◽  
Early Postpartum ◽  
Metabolite Profiles ◽  
Main Effect ◽  
Major Chemical

The objectives of this study were to characterize and compare non-volatile polar metabolite profiles of bovine colostrum, collected within 1 h and at 72 h after parturition, from crossbred Holstein cows raised in northeastern Thailand. The colostrum serum was characterized and compared using a non-targeted proton nuclear magnetic resonance (1H-NMR) technique combined with chemometric analysis. Results demonstrated that the main effect of post-parturition time provided a significant impact on the physical properties and major chemical constituents of colostrum, while the influence of farm origin and sampling month were likely undetectable. The 1H-NMR technique enabled to identify 45 non-volatile polar metabolites in the samples. Partial least-squares-discriminant analysis (PLS-DA) allowed discrimination of colostrum metabolome not only according to different times after parturition, but also the origins of the farm as well as sampling months. Differential metabolites were statistically identified as potential biomarkers accountable for the discrimination. Besides basic nutritive compounds (amino acids and sugars), several bioactive metabolites such as ascorbate, creatine, carnitine, choline, acetylcarnitine, N-acetylglucosamine, ornithine, orotate, and UPD-glucose could be successfully elucidated. Our finding reveals the application of non-targeted 1H-NMR metabolomics as an effective tool to assess the biomolecular profiles of bovine colostrum and their essential dynamics during the first three days after parturition.

scholarly journals An Evaluation of Phenolic Compounds, Carotenoids, and Antioxidant Properties in Leaves of South African Cultivars, Peruvian 199062.1 and USA's Beauregard

2021 ◽  
Vol 8 ◽  
Author(s):  
Charmaine J. Phahlane ◽  
Sunette M. Laurie ◽  
Tinotenda Shoko ◽  
Vimbainashe E. Manhivi ◽  
Dharini Sivakumar
Keyword(s):  
Phenolic Compounds ◽  
Sweet Potato ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Potato Cultivar ◽  
Hierarchical Cluster ◽  
Dry Weight ◽  
Potato Cultivars ◽  
Dicaffeoylquinic Acid ◽  
The Usa

In this study, leaves of sweet potato cultivars from South Africa (“Ndou,” “Bophelo,” “Monate,” and “Blesbok”), “Beauregard,” a sweet potato cultivar from the USA, and a Peruvian cultivar “199062. 1” were analyzed using UPLC/QTOF/MS and chemometrics, with the aim of characterizing the locally developed sweet potato cultivars and comparing them with already well-known established varieties on the market. A set of 13 phenolic compounds was identified. A partial least squares discriminant analysis, a hierarchical cluster analysis, and variables importance in projection were used to successfully distinguish sweet potato varieties based on their distinct metabolites. Caffeic acid enabled to distinguish Cluster 1 leaves of varieties (“Beauregard” and “Ndou”) from Cluster 2 (“199062.1,” “Bophelo,” “Monate,” and “Blesbok”). The leaves of “Bophelo” contained the highest concentrations of rutin, quercetin 3-O-galactoside, 3-caffeoylquinic acid (3-CQA), (5-CQA), 1,3 dicaffeoylquinic acid (1,3-diCQA), 1,4-diCQA, and 3,5-diCQA. Furthermore, Bophelo leaves showed the highest antioxidant activities (FRAP 19.69 mM TEACg−1 and IC50 values of (3.51 and 3.43 mg ml−1) for DPPH and ABTS, respectively, compared to the other varieties. Leaves of “Blesbok” contained the highest levels of β-carotene (10.27 mg kg−1) and zeaxanthin (5.02 mg kg−1) on a dry weight basis compared to all other varieties. This study demonstrated that the leaves of local cultivars “Bophelo” and “Blesbok” have the potential to become functional ingredients for food processing.

scholarly journals CHEMICAL CONSTITUENTS FROM THE SEEDS OF ANNONA RETICULATA L. IN VIETNAM

2018 ◽  
Vol 54 (4A) ◽  
pp. 221
Author(s):  
Nguyen Huy Hung
Keyword(s):  
Phenolic Compounds ◽  
1H Nmr ◽  
13C Nmr ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Heteronuclear Correlation ◽  
Chemical Structures ◽  
Eleutheroside B ◽  
Rotundic Acid ◽  
Annona Reticulata

In the present study, the chemical investigation of the seeds of A. reticulata L. (Annonaceae) in Vietnam has resulted in the identification of four known compounds, including two triterpenoids (rotundic acid (1), pedunculoside (2)), and two phenolic compounds (eleutheroside B (3), sinapaldehyde glucoside (4). Their chemical structures were elucidated on the basis of spectroscopic analysis, including homonuclear and heteronuclear correlation NMR (1H-NMR, 13C-NMR, COSY, HSQC, and HMBC) experiments.

scholarly journals Antioxidant Properties of Phenolic Compounds from Baccharis articulata and B. usterii

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Simone Quintana de Oliveira ◽  
Virgínia Demarchi Kappel ◽  
Viviane Silva Pires ◽  
Claiton Leoneti Lencina ◽  
Pascal Sonnet ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Antioxidant Effect ◽  
Trap Assay ◽  
Lead Compounds ◽  
Dicaffeoylquinic Acid ◽  
Total Antioxidant

As a continuation of our research on the Baccharis genus, the evaluation of the antioxidant effect by TRAP and TBARS assays of three isolated compounds from n-butanol fractions of B. articulata and B. usterii is reported. The structures of these compounds were established as 4- O-β-D-glucopyranosyl-3,5-dimethoxybenzyl-methanol (1), 5- O-[ E]-dicaffeoylquinic acid (2), and 7-hydroxy-5,4′-dimethoxyflavone (3). In the TRAP assay it was possible to observe an antioxidant effect of both n-butanol fractions at 1.25 μg/mL. Among the isolated compounds, compound 2 displayed a remarkable contribution to the total antioxidant capacity of the n-butanol fraction of B. usterii. Moreover, the n-butanol fractions of both species, compounds 1 and 2 at 40.0 μg/mL were efficient in protection of lipid peroxidation in the TBARS experiment. They are promising lead compounds for use in medicinal chemistry studies.

scholarly journals Natural Phenolic Compounds for Health, Food and Cosmetic Applications

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 427
Author(s):  
Lucia Panzella
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Food Supplements ◽  
Health Food ◽  
Functional Ingredients ◽  
Cosmetic Industry ◽  
Natural Phenolic Compounds

Based on their potent antioxidant properties, natural phenolic compounds have gained more and more attention for their possible exploitation as food supplements, as well as functional ingredients in food and in the cosmetic industry [...]

scholarly journals 1H-NMR Profiling Shows as Specific Constituents Strongly Affect the International EVOO Blends Characteristics: The Case of the Italian Oil

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2233
Author(s):  
Francesca Calò ◽  
Chiara Roberta Girelli ◽  
Federica Angilè ◽  
Laura Del Coco ◽  
Lucia Mazzi ◽  
...  
Keyword(s):  
1H Nmr ◽  
Metabolic Profile ◽  
1H Nmr Spectroscopy ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Minor Components ◽  
Multivariate Statistical ◽  
Olive Oils ◽  
The World ◽  
Plateau Level

Considering the growing number of extra virgin olive oil (EVOO) producers in the world, knowing the influence of olive oils with different geographical origins on the characteristics of the final blend becomes an interesting goal. The present work is focused on commercial organic EVOO blends obtained by mixing multiple oils from different geographical origins. These blends have been studied by 1H-NMR spectroscopy supported by multivariate statistical analysis. Specific characteristics of commercial organic EVOO blends originated by mixing oils from Italy, Tunisia, Portugal, Spain, and Greece were found to be associated with the increasing content of the Italian component. A linear progression of the metabolic profile defined characteristics for the analysed samples—up to a plateau level—was found in relation to the content of the main constituent of the Italian oil, the monocultivar Coratina. The Italian constituent percentage appears to be correlated with the fatty acids (oleic) and the polyphenols (tyrosol, hydroxytyrosol, and derivatives) content as major and minor components respectively. These results, which highlight important economic aspects, also show the utility of 1H-NMR associated with chemometric analysis as a powerful tool in this field. Mixing oils of different national origins, to obtain blends with specific characteristics, could be profitably controlled by this methodology.

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