scholarly journals Chemical and Skincare Property Characterization of the Main Cocoa Byproducts: Extraction Optimization by RSM Approach for Development of Sustainable Ingredients

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7429
Author(s):  
Catalina Agudelo ◽  
Karent Bravo ◽  
Ana Ramírez-Atehortúa ◽  
David Torres ◽  
Luis Carrillo-Hormaza ◽  
...  
Keyword(s):  
Pharmaceutical Formulations ◽  
Extraction Process ◽  
Skin Aging ◽  
Cocoa Bean ◽  
Cocoa Beans ◽  
Production Chain ◽  
Cocoa Production ◽  
Optimized Conditions ◽  
Property Characterization

Methylxanthines and polyphenols from cocoa byproducts should be considered for their application in the development of functional ingredients for food, cosmetic and pharmaceutical formulations. Different cocoa byproducts were analyzed for their chemical contents, and skincare properties were measured by antioxidant assays and anti-skin aging activity. Musty cocoa beans (MC) and second-quality cocoa beans (SQ) extracts showed the highest polyphenol contents and antioxidant capacities. In the collagenase and elastase inhibition study, the highest effect was observed for the SQ extract with 86 inhibition and 36% inhibition, respectively. Among cocoa byproducts, the contents of catechin and epicatechin were higher in the SQ extract, with 18.15 mg/100 g of sample and 229.8 mg/100 g of sample, respectively. Cocoa bean shells (BS) constitute the main byproduct due to their methylxanthine content (1085 mg of theobromine and 267 mg of caffeine/100 g of sample). Using BS, various influencing factors in the extraction process were investigated by response surface methodology (RSM), before scaling up separations. The extraction process developed under optimized conditions allows us to obtain almost 2 g/min and 0.2 g/min of total methylxanthines and epicatechin, respectively. In this way, this work contributes to the sustainability and valorization of the cocoa production chain.

scholarly journals PARTIAL CHARACTERIZATION of lipase from COCOA BEANS (Theobroma cacao. L.) of clone PBC 159

2010 ◽  
Vol 8 (3) ◽  
pp. 448-453
Author(s):  
Ratna Agung Samsumaharto
Keyword(s):  
Theobroma Cacao ◽  
Cocoa Bean ◽  
Cocoa Beans ◽  
Ph Optimum ◽  
Nitrobenzoic Acid ◽  
Lipase Enzyme ◽  
Wide Range ◽  
Km Value ◽  
Theobroma Cacao L

A study was carried out to characterize the cocoa lipase from cocoa beans (Theobroma cacao, L.) of clone PBC 159. The optimum temperature of cocoa lipase was 30-40 °C and the pH optimum was 7.0-8.0. The moleculer weight of the lipase enzyme was in between 45-66 kDa. The results indicate that Km value for cocoa bean lipase was 2.63 mM, when trimyristin was used as a substrate. The incubation of cocoa bean lipase with triolein and tributyrin (as substrate) yielded Km of 11.24 and 35.71 mM, respectively. The Vmax value obtained from the incubation of the lipase with a wide range of substrates, including tributyrin, trimyristin and triolein, are expressed as µmole acid/min/mg protein for cocoa lipase. Vmax values decreased with the increase in the triacylglycerol chain-length, with Vmax values of 27.78, 13.16 and 11.63 µmole acid/min/mg protein when incubated with tributyrin, trimyristin and triolein, respectively. Inhibition of lipase occurred in the presence of diisopropyl flourophosphate, N-bromosuccinimide and 5,5-dithiobis-(-2-nitrobenzoic acid).   Keywords: characterization, lipase, cocoa beans

scholarly journals Determination of Selection Index of Cocoa (Theobroma cacao L.) Yield Traits Using Regression Methods

2016 ◽  
Vol 32 (2) ◽  
pp. 101-108
Author(s):  
Bayu Setyawan ◽  
Taryono . ◽  
Suyadi Mitrowihardjo
Keyword(s):  
Plant Traits ◽  
Selection Index ◽  
Dry Weight ◽  
High Yield ◽  
Cocoa Bean ◽  
Fresh Weight ◽  
Cocoa Beans ◽  
Central Java ◽  
Cocoa Production ◽  
Planting Materials

The increasing chocolate consumption has not been followed by growing production of dry cocoa beans. In order to support the increase in cocoa production, planting materials with high yield are needed. The objective of this research was to determine the components of cocoa traits affecting weight of dry cocoa beans, and set a selection index for superior cocoa trees. The experiment material were four cocoa hybrid populations of which their family ancestry were unknown, and were planted on Samigaluh Plantation, Yogyakarta, and Segayung Plantation, Central Java. Observations and data collection were conducted on four plant populations. The observations were undertaken for three years, by observing plant traits, including pod length, pod diameter, husk thickness, cavity diameter, pod fresh weight, cocoa bean/pod fresh weight, husk fresh weight, dry weight of cocoa beans/pod, number of cocoa beans/pod, dry weight per cocoa bean. The collected data were analyzed using path and regression analysis methods. The results showed that pod diameter (X4), fresh pod weight (X5), number of cocoa beans/pod (X8), and dry weight/cocoa bean (X9) were used to form a selection index resulting the equation I = 0.0792 X4 + 0.1330 X5 + 0.0106 X8 + 0.1349 X9 furthermore will be used in the selection of cocoa trees. Ten cocoa plants from seeds having the highest general selection index were D 034, D 003, D 015, A 054, D 004, D 033, D 041, A 157, D 036, and D 025 will be selected for further evaluation.

scholarly journals Characterization of Polyphenols from Various Cocoa (Theobroma cacao L.) Clones During Fermentation

2018 ◽  
Vol 34 (2) ◽  
pp. 104-112
Author(s):  
Muhammad Isa Dwijatmoko ◽  
Budi Nurtama ◽  
Nancy Dewi Yuliana ◽  
Misnawi Misnawi
Keyword(s):  
Theobroma Cacao ◽  
Antioxidant Property ◽  
Cocoa Bean ◽  
Flavor Quality ◽  
Cocoa Beans ◽  
Total Polyphenols ◽  
One Step ◽  
Theobroma Cacao L

Cocoa bean is a rich source of polyphenols, which are the largest group secondary metabolite with natural antioxidant property. Polyphenols from cocoabeans was reported to possess health benefits. Fermentation, one step in cocoa processing is needed to improve the quality of cocoa in which the concentration of cocoa bean polyphenols might decrease significantly through oxidation and exudation. Cocoa polyphenols content among different cocoa clones mightalso vary. The aims of this study were to determine total polyphenols, total flavanoid, epicatechin, and catechin content in several cocoa clones, those wereSulawesi 1, Sulawesi 2, ICCRI 03, and KW 617. Until now, characterization of polyphenols from those clones has not been reported. The effect of five daysfermentation to those parameters was also studied. The results of the study showed that fermentation and type of clones significantly affected total of polyphenols, total of flavanoids, epicatechin, and catechin content of the cocoa, there is also an interaction between fermentation and type of clones. Unfermented of Sulawesi 1 had the highest total polyphenols of 96.94±5.83 mg/g, total flavanoids of 90.92±1.89 mg/g, epicatechin of 52.50±0.46 mg/g, and catechin of 1.99±0.02 mg/g content compared to other clones. Among five days fermented cocoa beans, Sulawesi 2 showed the highest total polyphenols and total flavanoids content, while ICCRI 03 had the highest epicatechin and catechin content than other clones. Thus, in can be concluded that although fermentation is required to improve the flavor quality of cocoa, it significantly reduced the content of bioactive compounds. This effect varied amongdifferent cocoa clones.

scholarly journals Sustainability of cocoa production in Indonesia

2020 ◽  
pp. 997-1003
Author(s):  
Muhardi ◽  
Abdul Rahim ◽  
Effendy ◽  
Made Antara ◽  
Rustam Abdul Rauf ◽  
...  
Keyword(s):  
Ecological Factors ◽  
Survey Method ◽  
Cocoa Bean ◽  
Cocoa Beans ◽  
Technological Factors ◽  
Cocoa Production ◽  
Farming Families ◽  
Farmer Education ◽  
Made In

The objective of this study was to present empirical evidence about the sustainability of cocoa farming in Indonesia and how decisions are made in producing cocoa beans. This study used a survey method involving a questionnaire for collecting data. The results showed that the sustainability of cocoa farming was determined by weak sustainable ecological factors (46.07%), moderately sustainable socioeconomic factors (54.43%), moderately sustainable technological factors (55.95%), moderately sustainable factors that help farmers in cocoa farming (59.60%), and weak sustainable factors in cocoa farming families' futures (47.52%). To increase the sustainability of cocoa farming, the current study found that farmer education, cocoa crop rejuvenation, cocoa pest and disease control, cleanliness and quality of cocoa beans, cocoa productivity, institutions, extension, technology, credit availability, and stability of cocoa bean prices are the most important factors to be improved.

scholarly journals Peningkatan Kualitas dan Kuantitas Biji Kakao Melalui Intensifikasi Perawatan Kakao, Introduksi Alat Budidaya, dan Pengering Sistem Hybrid

2021 ◽  
Vol 3 (1) ◽  
pp. 8
Author(s):  
Mercy Bientri Yunindanova ◽  
Dimas Rahadian Aji Muhammad ◽  
Sigit Prabawa
Keyword(s):  
Positive Impact ◽  
Cocoa Bean ◽  
Cocoa Beans ◽  
Plant Maintenance ◽  
Cocoa Production ◽  
Maintenance Activities ◽  
Cacao Plantation ◽  
Cacao Plant ◽  
Drying Chamber ◽  
Production Industry

Putat village, Patuk district, Gunung Kidul regency is one of cocoa production center in Yogjakarta. Cocoa bean products in this region have been marketed not only in Yogjakarta but also several areas in Indonesia. In order to support production continuity of cocoa bean productions, to date the farmers face obtacles in cacao plantation maintenance and cocoa beans drying. Thus, this activity aimed to increase quality and quantity of cocoa beans through maintenance intensifications of cacao and introduction of cacao cultivation equipments and cocoa beans drying hybrid system. Partner in this activities were Kelompok Tani Kakao Sido Dadi and cocoa dan chocolate production industry “Joglo Coklat” located in Patuk, Gunung Kidul, Yogjakarta. The activities were conducted in several stages including 1) basic data collection, 2) socialization of cacao plantation maintenance, 3) socialization of cocoa beans drying, 4) introduction of cultivation equipments, 5) contruction of drying chamber, 6) handover of equipment, and 7) evaluation of parnter response. The results of the activity indicated that the provision of information and the introduction of equipment for cacao plant maintenance activities had a positive impact on partners. From the questionnaires, the partners stated the benefits of this activity and the desire for sustainable activities. The introduction of maintenance equipment and cocoa beans drying made it easier for farmers to carry out cultivation, harvest and post-harvest activities of cocoa beans so would be able to improve the quality and quantity of cocoa beans.

scholarly journals OPTIMIZATION OF ULTRASOUND ASSISTED EXTRACTION OF POLYPHENOLS IN COCOA BEANS

Revista Vitae ◽  
2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Jonny Ariel Fajardo Daza ◽  
Frank Carlos Herrera Ruales ◽  
Carol Andrea Ibarra Cabrera
Keyword(s):  
Antioxidant Properties ◽  
Extraction Process ◽  
Design Stage ◽  
Cocoa Bean ◽  
Solvent Ratio ◽  
Cocoa Beans ◽  
Ultrasound Assisted Extraction ◽  
Optimal Point ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Background: The determination of polyphenols in cocoa beans allows the establishing of antioxidant properties of great benefit to this product. Objectives: Optimization of the ultrasound-assisted extraction method to determine the content of polyphenols in cocoa beans. Methods: Two experiments design stages were carried out to optimize the ultrasound-assisted extraction process of polyphenols from the cocoa bean. In the first experiment design stage, the adequate extraction solvent was determined; for this purpose, five types of solvents were evaluated through a completely random design unrestricted (CRD). In the second experiment stage, a central composite design 22 + star point (with two central points) was used, which was evaluated using the response surface methodology to determine the influence of the temperature, time, and solute / solvent ratio. Results: The experiment found that acetone: water: acetic acid (70: 29.5: 0.5) mixture, leads to a greater amount of total extracted phenols measured for the Folin-Ciocalteu method. Analysis of variance (ANOVA) found that six significant effects that influence the response variable (total phenols extracted). The main effects were of the three factors and three of their interactions. Conclusions: After the optimizing said factors, an optimal point was found: 39.3 °C oftemperature, 74.5 minutes, and 22.8 mL of solvent per gram of cocoa sample.

Determination of the Prospective Processed Cacao Product and Calculation of the Added Value in Agro-Tourism Based on Cacao Agroindustry in Pidie Jaya Regency

2018 ◽  
Vol 22 (1) ◽  
pp. 33-42
Author(s):  
◽  
Tajuddin Bantacut ◽  
Sapta Raharja
Keyword(s):  
System Approach ◽  
Added Value ◽  
Assessment Criteria ◽  
Cocoa Bean ◽  
Cocoa Beans ◽  
Bayes Method ◽  
Cocoa Products ◽  
Processed Products ◽  
Derivative Products

Abstract Utilization of cocoa bean to be a derivative products in industrial is wide enough, that it is necessary to determine the priority of the processed products development. This study aimed to determine the prospective processed cocoa products with a system approach using Bayes method and assessed the potential of added value by using Hayami method. Based on several assessment criteria indicated that chocolate bar is the priority product that needs to be developed and followed by several other processed products. This development was able to produce the added value of Rp 135.000 per kg of cocoa beans. Result indicated that by processing the cocoa beans into chocolate bar could provide a considerable income for the businessman.

Hydrogel-clay Nanocomposites as Carriers for Controlled Release

2020 ◽  
Vol 27 (6) ◽  
pp. 919-954 ◽  
Author(s):  
Raluca Ianchis ◽  
Claudia Mihaela Ninciuleanu ◽  
Ioana Catalina Gifu ◽  
Elvira Alexandrescu ◽  
Cristina Lavinia Nistor ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Hybrid Materials ◽  
Pharmaceutical Formulations ◽  
Tree Of Life ◽  
Present Review ◽  
Clay Nanocomposites ◽  
Delivery Platform ◽  
Complex Hybrid

The present review aims to summarize the research efforts undertaken in the last few years in the development and testing of hydrogel-clay nanocomposites proposed as carriers for controlled release of diverse drugs. Their advantages, disadvantages and different compositions of polymers/biopolymers with diverse types of clays, as well as their interactions are discussed. Illustrative examples of studies regarding hydrogel-clay nanocomposites are detailed in order to underline the progressive researches on hydrogel-clay-drug pharmaceutical formulations able to respond to a series of demands for the most diverse applications. Brief descriptions of the different techniques used for the characterization of the obtained complex hybrid materials such as: swelling, TGA, DSC, FTIR, XRD, mechanical, SEM, TEM and biology tests, are also included. Enlightened by the presented data, we can suppose that hydrogel-clay nanocomposites will still be a challenging subject of global assiduous researches. We can dare to dream to an efficient drug delivery platform for the treatment of multiple affection concomitantly, these being undoubtedly like ”a tree of life” bearing different kinds of fruits and leaves proper for human healing.

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