scholarly journals Synthesis, Cytotoxicity Assessment and Optical Properties Characterization of Colloidal GdPO4:Mn2+, Eu3+ for High Sensitivity Luminescent Nanothermometers Operating in the Physiological Temperature Range

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 421 ◽  
Author(s):  
Kamila Maciejewska ◽  
Blazej Poźniak ◽  
Marta Tikhomirov ◽  
Adrianna Kobylińska ◽  
Łukasz Marciniak
Keyword(s):  
Synthesis Method ◽  
High Sensitivity ◽  
Relative Sensitivity ◽  
Spectroscopic Properties ◽  
Physiological Temperature ◽  
Temperature Range ◽  
Cytotoxicity Studies ◽  
Cytotoxicity Assessment

Herein, a novel synthesis method of colloidal GdPO4:Mn2+,Eu3+ nanoparticles for luminescent nanothermometry is proposed. XRD, TEM, DLS, and zeta potential measurements confirmed the crystallographic purity and reproducible morphology of the obtained nanoparticles. The spectroscopic properties of GdPO4:Mn2+,Eu3+ with different amounts of Mn2+ and Eu3+ were analyzed in a physiological temperature range. It was found that GdPO4:1%Eu3+,10%Mn2+ nanoparticles revealed extraordinary performance for noncontact temperature sensing with relative sensitivity SR = 8.88%/°C at 32 °C. Furthermore, the biocompatibility and safety of GdPO4:15%Mn2+,1%Eu3+ was confirmed by cytotoxicity studies. These results indicated that colloidal GdPO4 doped with Mn2+ and Eu3+ is a very promising candidate as a luminescent nanothermometer for in vitro applications.

Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus

2020 ◽  
Vol 21 ◽  
Author(s):  
Muhammad Arfat Yameen ◽  
Amir Zeb ◽  
Raza E Mustafa ◽  
Sana Mushtaq ◽  
Nargis Aman ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Hybrid Material ◽  
Ag Nanoparticles ◽  
Hybrid Composite ◽  
Synthesis Method ◽  
Biological Evaluation ◽  
Vitro Assay ◽  
Cytotoxicity Studies ◽  
Material Composite

Background: Incoherent use of antibiotics has led toward resistance in MRSA, which is becoming multidrugresistant with high rate of virulence in the community and hospital settings. Objective: Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles and chitosan which were prepared by one-step synthesis method and various characterizations were performed. Methods: Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques while agar well diffusion was used for hybrid composite spheres. The in vitro and cytotoxicity studies was done by skin abrasion mouse model and MTT assay on RD cell respectively. Results: All isolates were resistant with the tested antibiotics except vancomycin. MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1. The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm respectively. Morphology of spheres was uneven, varied, porous and irregular in SEM and Ag nanoparticles presence and formation was also seen in micrograph. No substantial interface among drug, nanoparticles and polymer was found in XRD and IR showed characteristic peaks of all compound in the formulation. The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm). A significant reduction in microbial burden (~6.5 log10 CFU ml-1) was seen in vivo with loaded hybrid composite spheres formulation. The MTT assay indicated no potential cytotoxicity with hybrid composite spheres. Conclusion: Synergistic effect, amoxicillin, new hybrid formulation, anti-MRSA activity, composite spheres. nanoparticles.

scholarly journals Development and characterization of PLGA nanocapsules of grandisin isolated from Virola surinamensis: in vitro release and cytotoxicity studies

2013 ◽  
Vol 23 (1) ◽  
pp. 153-159 ◽  
Author(s):  
Luciano Aparecido Stecanella ◽  
Stephânia Fleury Taveira ◽  
Ricardo Neves Marreto ◽  
Marize C. Valadares ◽  
Marcelo de Sousa Vieira ◽  
...  
Keyword(s):  
In Vitro Release ◽  
Cytotoxicity Studies ◽  
Vitro Release

Synthesis and studies of thiazolidinedione–isatin hybrids as α-glucosidase inhibitors for management of diabetes

2021 ◽  
Vol 13 (5) ◽  
pp. 457-485
Author(s):  
Ramandeep Kaur ◽  
Rajnish Kumar ◽  
Nilambra Dogra ◽  
Ashok Kumar ◽  
Ashok Kumar Yadav ◽  
...  
Keyword(s):  
In Silico ◽  
Binding Free Energy ◽  
Biological Evaluation ◽  
Free Energy Calculations ◽  
Standard Drug ◽  
Glucosidase Inhibitors ◽  
Cytotoxicity Studies ◽  
Cytotoxicity Assessment

Aim: Keeping in view the side effects associated with clinically used α-glucosidase inhibitors, novel thiazolidinedione–isatin hybrids were synthesized and evaluated by in vitro, in vivo and in silico procedures. Materials & methods: Biological evaluation, cytotoxicity assessment, molecular docking, binding free energy calculations and molecular dynamics studies were performed for hybrids. Results: The most potent inhibitor A-10 (IC50 = 24.73 ± 0.93 μM) was competitive in manner and observed as non-cytotoxic. A-10 possessed higher efficacy than the standard drug (acarbose) during in vivo biological testing. Conclusion: The enzyme inhibitory potential and safety profile of synthetic molecules was recognized after in vitro, in vivo, in silico and cytotoxicity studies. Further structural optimization of A-10 can offer potential hit molecules suitable for future investigations.

scholarly journals Biosynthesis and Characterization of ZnO: Ag2O Nanocomposite for Antifungal Efficacy

2021 ◽  
Vol 2114 (1) ◽  
pp. 012081
Author(s):  
Ali Abdullah Fayyadh ◽  
Muneer H. Jaduaa Alzubaidy
Keyword(s):  
Plant Extract ◽  
Synthesis Method ◽  
Spectroscopic Properties ◽  
Zinc Nitrate ◽  
Biological Synthesis ◽  
Nitrate Hexahydrate ◽  
Lawsonia Inermis ◽  
X Ray ◽  
Inhibitory Efficiency

Abstract In this study, biosynthesis zinc and silver oxide nanocomposite (Bs-ZANc) were prepared using an eco-friendly biological synthesis method using silver nitrate, zinc nitrate hexahydrate, and Lawsonia inermis (Henna) plant extract with four different concentrations; (0.1, 0.2, 0.3, and 0.4) molar. The detailed characterization of Bs-ZANc was performed using Grazing X-ray diffraction technique (G-XRD), Field Emission Scanning Electron Microscope (FE-SEM), X-ray energy dispersive spectroscopy (EDX), Zeta Potential (Z.P.), and Dynamic Light Scattering (DLS). In addition to studying the spectroscopic properties using Fourier transform infrared spectroscopy (FTIR). The result showed good inhibitory efficiency of Bs-ZANc against some types of fungal such as; Penicillium spp., Aspergillus spp., and Candida Albicans. Further, a comparison was made between Bs-ZANc and Lawsonia inermis (Henna) plant extract only regarding antfungal efficacy.

scholarly journals Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protei

2020 ◽  
Author(s):  
Meng Xu ◽  
Feifeng Lu ◽  
Qingping Wu ◽  
Jumei Zhang ◽  
Peng Tian ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
High Sensitivity ◽  
Relative Sensitivity ◽  
Immunochromatographic Assay ◽  
Clinical Samples ◽  
Special Equipment ◽  
Human Noroviruses ◽  
Infected People ◽  
Sensitivity Specificity

Abstract Background: Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all ages worldwide. As the replication of HuNoVs in vitro is immature, the detection of HuNoVs is depended on molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced operators to perform, and extended time to get results. In addition, the genotypes of HuNoVs are broad. Therefore, a method for rapidly, broad-range, and effective approach for HuNoVs would be more applicable for screening infected people when outbreaks occur.Results: In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for highly effective detection of HuNoVs in clinical samples. Monoclonal antibodies (McAbs) against the shell (S) domain in the major capsid protein of HuNoV were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2×106 genomic copies per gram (gc/g) and 4.4×105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 samples were tested for HuNoVs by ICA and compared against that by RT-qPCR. The relative sensitivity, specificity and agreement of the ICA was 84.2 % (95% CI: 83.6-84.8 %), 100.0 % (95 % CI: 98.5-100.0 %) and 87.7 % (95% CI: 85.6-89.8 %), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA could detect a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs.Conclusions: Our results demonstrated that ICA targeting the S domain protein is a promising candidate for effectively improve identifying different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

Nanostructured TiN-Coated Electrodes for High-Sensitivity Noninvasive Characterization of in Vitro Tissue Models

2018 ◽  
Vol 1 (5) ◽  
pp. 2284-2293 ◽  
Author(s):  
Tobias Schmitz ◽  
Matthias Schweinlin ◽  
Robin T. Kollhoff ◽  
Lisa Engelhardt ◽  
Christian Lotz ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Noninvasive Characterization ◽  
Tissue Models ◽  
Coated Electrodes

Design and Synthesis of an MOF Thermometer with High Sensitivity in the Physiological Temperature Range

2015 ◽  
Vol 54 (23) ◽  
pp. 11193-11199 ◽  
Author(s):  
Dian Zhao ◽  
Xingtang Rao ◽  
Jiancan Yu ◽  
Yuanjing Cui ◽  
Yu Yang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Physiological Temperature ◽  
Temperature Range ◽  
Design And Synthesis

scholarly journals Encapsulation of Babchi Oil in cyclodextrin-Based Nanosponges: Physicochemical Characterization, Photodegradation and In Vitro Cytotoxicity Studies

2018 ◽  
Author(s):  
Sunil Kumar ◽  
Pooja Sihag ◽  
Francesco Trotta ◽  
Rekha Rao
Keyword(s):  
Essential Oil ◽  
Physicochemical Characterization ◽  
X Ray Diffraction ◽  
Traditional Medicines ◽  
Molar Ratios ◽  
Beta Cyclodextrin ◽  
Cyclodextrin Nanosponges ◽  
Cytotoxicity Studies

Babchi (Psoralea corylifolia) oil is an important essential oil used in several traditional medicines to cure various disorders. This phytotherapeutic agent possesses number of pharmacological activities including antibacterial, antifungal, antioxidant, anti-inflammatory, immunomodulatory and antitumor. However, volatile nature, poor stability and solubility of babchi oil (BO) restrict its pharmaceutical applications. Hence, the aim of the present work was to encapsulate this oil in β-cyclodextrin nanosponges (NS) in order to overcome above limitations. To fabricate nanosponges, β-cyclodextrin was crosslinked with diphenyl carbonate in different molar ratios viz.1:2, 1:4, 1:6, 1:8 and 1:10. The blank nanosponges were loaded with babchi oil using freeze-drying method. Particle size of the babchi oil loaded nanosponges was found to lie between 200-500 nm, with low polydispersity index. Further, zeta potential, Fourier transform infrared spectroscopy, X-ray diffraction, thermal analysis and electron microscopy were carried out for characterization of babchi oil nanosponges. Results obtained from spectral analysis ascertained the formation of inclusion complexes. Additionally, solubilisation efficiency of the babchi oil was checked in distilled water and found enhanced by 4.95 times with optimized β-cyclodextrin nanosponges. The cytotoxicity study was carried out by MTT assay using HaCaT cell lines. A significant improvement in photostability of essential oil was also observed by inclusion in   nanosponges. Lastly, the optimized formulation was tested for antibacterial activity using Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Hence, encapsulation of BO in nanosponges resulted in efficacious carrier system in terms of solubility, photostability as well as safety of this oil along with handling benefits.

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