scholarly journals Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 462 ◽  
Author(s):  
Raghavendra Ramalingam ◽  
Chetna Dhand ◽  
Chak Leung ◽  
Hariharan Ezhilarasu ◽  
Praseetha Prasannan ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Bacterial Colonization ◽  
Parenchymal Cell ◽  
Antimicrobial Properties ◽  
Recovery Process ◽  
Dermal Fibroblasts ◽  
Microbial Colonization ◽  
Herbal Extract ◽  
Burst Release ◽  
Gymnema Sylvestre

Electrospun fibers have emerged as promising materials in the field of biomedicine, due to their superior physical and cell supportive properties. In particular, electrospun mats are being developed for advanced wound dressing applications. Such applications require the firers to possess excellent antimicrobial properties in order to inhibit potential microbial colonization from resident and non-resident bacteria. In this study, we have developed Poly-ε-Caprolactone /gelatin hybrid composite mats loaded with natural herbal extract (Gymnema sylvestre) to prevent bacterial colonization. As-spun scaffolds exhibited good wettability and desirable mechanical properties retaining their fibrous structure after immersing them in phosphate buffered saline (pH 7.2) for up to 30 days. The initial burst release of Gymnema sylvestre prevented the colonization of bacteria as confirmed by the radial disc diffusion assay. Furthermore, the electrospun mats promoted cellular attachment, spreading and proliferation of human primary dermal fibroblasts and cultured keratinocytes, which are crucial parenchymal cell-types involved in the skin recovery process. Overall these results demonstrated the utility of Gymnema sylvestre impregnated electrospun PCL/Gelatin nanofibrous mats as an effective antimicrobial wound dressing.

scholarly journals To Evaluate Antimicrobial Properties of Platelet Rich Plasma and Source of Colonization in Pressure Ulcers in Spinal Injury Patients

Ulcers ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Roop Singh ◽  
Raj Kumar Dhayal ◽  
Paramjit Kumar Sehgal ◽  
Rajesh K. Rohilla
Keyword(s):  
Staphylococcus Aureus ◽  
Pressure Ulcers ◽  
Spinal Injury ◽  
Platelet Rich Plasma ◽  
Bacterial Colonization ◽  
Antimicrobial Properties ◽  
Local Environment ◽  
Microbial Colonization ◽  
Local Application ◽  
Cord Injury

Background. Exposure of pressure ulcers (PrUs), particularly to urine and feces, leads to increased colonization of wounds. The aim of the present study was to evaluate the source of microbial colonization and antimicrobial properties of autologous platelet rich plasma (PRP) in controlling it in PrUs. Methods. Twenty-five patients of spinal cord injury (SCI) with at least two PrUs were taken for the study. Local application of autologous PRP on one PrU (case) was compared with saline dressing on the other PrU (control). Urine cultures, urethral meatus, PrUs, and perineal swabs were taken at weekly interval for five weeks. Result. Colonization rate of PrUs (case) decreased from 92% at enrollment to 24% at the 5th week but did not significantly decrease in PrUs (control) from enrollment (84%) to the 5th week (76%). Association between PrU (case) and perineal cultures was observed for Staphylococcus aureus at enrollment 41% (χ2=6.76, P<0.01) and at the 2nd week 47% (χ2=5.83, P<0.05). 47% association between PrU (control) and perineal cultures at enrollment (χ2=4.11, P<0.05) and 29% association at the 2nd week (χ2=8.41, P<0.01) were observed for Staphylococcus aureus. There was association between bacteria present in perineum/urine and those colonizing PrUs. Conclusion. There is a significant association between PrUs colonization and bacteria present in local environment (urine and feces). Local application of autologous PRP changes the “biological milieu” of the PrUs through its antimicrobial properties leading to reduction in bacterial colonization.

scholarly journals Antimicrobial Properties and Release Profile of Ampicillin from Electrospun Poly(εepsilon;-caprolactone) Nanofiber Yarns

2010 ◽  
Vol 5 (4) ◽  
pp. 155892501000500 ◽  
Author(s):  
Hang Liu ◽  
Karen K. Leonas ◽  
Yiping Zhao
Keyword(s):  
In Vitro Release ◽  
Antimicrobial Properties ◽  
Fiber Surface ◽  
Electrospun Nanofibers ◽  
Release Profile ◽  
Burst Release ◽  
Spun Yarns ◽  
Surgical Sutures ◽  
Release Model

Poly(εepsilon;-caprolactone) (PCL) electrospun fibers containing ampicillin sodium salt have been produced and twisted into nanofiber yarns. The fiber diameters and crystallinity, the in vitro antimicrobial properties of the yarns, and the in vitro release of ampicillin from yarns containing various ampicillin concentrations are studied. Decreased fiber diameters and reduced diameter variation are observed with the addition of ampicillin salt into the polymer solution. The results from the zone of inhibition test of the yarns against both gram-positive Staphylococcus aureus and gram-negative Klebsiella pneumoniae indicate that the released ampicillin retains its effectiveness after the production processes, therefore the as-spun yarns are antimicrobial active. A burst release of ampicillin from the yarns has been observed in the first hour, and the release is almost completed in 96 hours. The burst release is believed to be due to the low compatibility of ampicillin with PCL, the accumulation of ampicillin on fiber surface and the small fiber diameters. An empirical release model is developed to describe the release profile. The results indicate that the electrospun nanofibers yarns will have a great potential to be used for biomaterials, such as surgical sutures, to decrease the surgical site infection rate.

All-natural composite wound dressing films of essential oils encapsulated in sodium alginate with antimicrobial properties

2014 ◽  
Vol 463 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Ioannis Liakos ◽  
Loris Rizzello ◽  
David J. Scurr ◽  
Pier Paolo Pompa ◽  
Ilker S. Bayer ◽  
...  
Keyword(s):  
Essential Oils ◽  
Sodium Alginate ◽  
Wound Dressing ◽  
Antimicrobial Properties ◽  
Natural Composite

scholarly journals Phenotypic and clonal stability of antigen-inexperienced memory-like T cells across the genetic background, hygienic status, and aging

2020 ◽  
Author(s):  
Alena Moudra ◽  
Veronika Niederlova ◽  
Jiri Novotny ◽  
Lucie Schmiedova ◽  
Jan Kubovciak ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Genetic Background ◽  
Expression Profiles ◽  
Bacterial Colonization ◽  
Gene Expression Profiles ◽  
Inbred Strains ◽  
Microbial Colonization ◽  
Independent Manner ◽  
Different Genetic Background

AbstractAntigen-inexperienced memory-like T (AIMT) cells are functionally unique T cells representing one of the two largest subsets of murine CD8+ T cells. However, differences between laboratory inbred strains, insufficient data from germ-free mice, a complete lack of data from feral mice, and unclear relationship between AIMT cells formation during aging represent major barriers for better understanding of their biology. We performed a thorough characterization of AIMT cells from mice of different genetic background, age, and hygienic status by flow cytometry and multi-omics approaches including analyses of gene expression, TCR repertoire, and microbial colonization. Our data showed that AIMT cells are steadily present in mice independently of their genetic background and hygienic status. Despite differences in their gene expression profiles, young and aged AIMT cells originate from identical clones. We identified that CD122 discriminates two major subsets of AIMT cells in a strain-independent manner. Whereas thymic CD122LOW AIMT cells (innate memory) prevail only in young animals with high thymic IL-4 production, peripheral CD122HIGH AIMT cells (virtual memory) dominate in aged mice. Co-housing with feral mice changed the bacterial colonization of laboratory strains, but had only minimal effects on the CD8+ T-cell compartment including AIMT cells.

scholarly journals Production and Characterization of Keratin/Tragacanth Gum Nanohydrogels for Drug Delivery in Medical Textiles

2021 ◽  
Vol 8 ◽  
Author(s):  
Nazanin Mansouri Shirazi ◽  
Niloofar Eslahi ◽  
Adeleh Gholipour-Kanani
Keyword(s):  
Disulfide Bonds ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Herbal Extract ◽  
Minimum Size ◽  
Mechanical Resistance ◽  
Tragacanth Gum ◽  
Medical Textiles

Keratin protein has been applied for biomedical applications due to its biocompatibility, biodegradability, mechanical resistance, and bioavailability. Tragacanth gum (TG) as a polysaccharide-based biopolymer has wound healing and antimicrobial properties. In this study, keratin was extracted from protein-based chicken feather by using reduction hydrolysis (sodium sulfide), and nanogels of keratin and TG composites at different ratios were produced by using the chemical cross-linking method. Then, cinnamon (5 and 10%) as an antibacterial herbal extract was added to the nanogels and coated on cotton fabric. The morphology and size of the composite nanogels, chemical structure, biological, and antibacterial properties were evaluated. According to DLS results, TGK2:1 (ratio of TG to keratin = 2:1) had the minimum size (80 nm) and PDI (0.1), and therefore, this sample was chosen as the optimum one. FESEM and TEM images showed the semi-spherical shape of the produced nanogels. FTIR spectra revealed the possible hydrogen bonding between the components, and the formation of disulfide bonds after the addition of hydrogen peroxide was confirmed by XPS. After loading cinnamon into the nanogels, an increase in size was observed from 80 nm for free-nanogel to 85 and 105 nm for 5 and 10% extract-loaded nanogels, respectively. Besides, more cinnamon was released from the treated fabrics by increasing time and cinnamon concentration. The antibacterial test exhibited good antibacterial properties against both Gram-positive and Gram-negative bacteria. Finally, MTT assay approved the biocompatibility of the produced nanogels for potential use in medical textiles.

Synthesis, characterization, and antimicrobial properties of novel dual drug loaded electrospun mat for wound dressing applications

2021 ◽  
Vol 36 (5) ◽  
pp. 431-443
Author(s):  
Swetha Andra ◽  
Satheesh kumar Balu ◽  
Rajalakshmi Ramamoorthy ◽  
Murugesan Muthalagu ◽  
Devisri Sampath ◽  
...  
Keyword(s):  
Wound Healing ◽  
Drug Release ◽  
Wound Dressing ◽  
High Surface ◽  
High Surface Area ◽  
Antimicrobial Properties ◽  
Sustained Drug Release ◽  
Gram Positive Bacteria ◽  
Antimicrobial Efficiency ◽  
Cissus Quadrangularis

Wound healing properties of some herbs have been known for decades. Recently, electrospun mats have been used as a wound dressing material due to the high surface area of fiber and ease of incorporation of drug into the fiber matrix. In this aspect, the incorporation of herbal extracts in electrospun matrix could provide synergistic effect for wound healing. In the present work, extracts from Cissus quadrangularis (CQ) and Galinsoga parviflora Cav (GP) were loaded into the PVA solution in different proportions. These solutions were used to produce nanofibrous mat in electrospinning and the characteristics of the mat were analyzed. The morphology of the fiber was analyzed using scanning electron microscope (SEM), the presence of functional groups was identified using Fourier transform infrared spectroscopy (FTIR). The result of drug release shows that the GP extract loaded PVA nanofibrous mat has sustained drug release of 28% after 8 h of incubation compared to CQ loaded PVA nanofibrous mat. This trend follows as the concentration of GP increases in the mixture. The antimicrobial efficiency of the prepared mat was evaluated against both Gram-negative bacteria E. coli and Gram-positive bacteria S. aureus. The prepared nanofibrous mat has shown excellent antibacterial activity, cell viability, hemocompatibility, and sufficient tensile properties which indicates that it could be a promising biomaterial for wound dressing application.

scholarly journals Lactobacillus rhamnosus GG and Biochemical Agents Enrich the Shelf Life of Fresh-Cut Bell Pepper (Capsicum annuum L. var. grossum (L.) Sendt)

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1252
Author(s):  
Kandasamy Saravanakumar ◽  
Anbazhagan Sathiyaseelan ◽  
Arokia Vijaya Anand Mariadoss ◽  
Ramachandran Chelliah ◽  
Xiaowen Hu ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Antioxidant Properties ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Bell Pepper ◽  
Microbial Colonization ◽  
Lactobacillus Rhamnosus Gg ◽  
Combined Application ◽  
Fresh Cut

This work analyzed the individual and combined effects of biochemical additives and probiotic strain Lactobacillus rhamnosus GG on red and yellow fresh-cut bell pepper (R- and Y-FCBP, respectively) stored at two different temperatures (4 °C and 15 °C) for 15 days. The results revealed that the combined application of biochemical additives and L. rhamnosus GG inhibited the colonization of total bacterial counts (25.10%), total Salmonella counts (38.32%), total Listeria counts (23.75%), and total fungal counts (61.90%) in FCBP. Total bacterial colonization was found to be higher in R-FCBP (1188.09 ± 9.25 CFU g−1) than Y-FCBP (863.96 ± 7.21 CFU g−1). The storage at 4 °C was prevented 35.38% of microbial colonization in FCBP. Importantly, the L. rhamnosus GG count remained for up to 12 days. Moreover, the combined inoculation of the biochemical additives and L. rhamnosus GG treatments (T3) maintained the quality of R- and Y-FCBP for up to 12 days at 4 °C without any loss of antioxidant properties. This work reports the successful utilization of L. rhamnosus GG as a preservative agent for maintaining the quality of FCBP by preventing microbial colonization.

scholarly journals Bi-layered Nanofibers Membrane Loaded with Titanium Oxide and Tetracycline as Controlled Drug Delivery System for Wound Dressing Applications

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1602 ◽  
Author(s):  
Rezk ◽  
Lee ◽  
Son ◽  
Park ◽  
Kim
Keyword(s):  
Titanium Oxide ◽  
Wound Dressing ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Cell Counting ◽  
Confocal Laser ◽  
Burst Release ◽  
Electrospinning Technique ◽  
Cell Compatibility

: The aim of this study is to develop a novel functional bi-layered membrane loaded titanium oxide (TiO2) and tetracycline (TTC) for application in wound dressing. The advantages of the electrospinning technique have to be considered for the uniform distribution of nanoparticles and TTC drug. The as prepared nanofibers and TiO2 were characterized in terms of morphology, fiber diameter, mechanical properties and surface wettability. The in vitro drug release study revealed initial burst release followed by a sustained control release of TTC for 4 days. The in vitro antibacterial of the bi-layered nanofibers was conducted against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria species showing excellent antibacterial effect for drug loaded samples compared with PCL nanofibers. Subsequently, cell counting kit-8 (CCK-8) and confocal laser scanning microscopy (CLSM) were used to evaluate its biocompatibility in vitro. Our results revealed that the bi-layered membrane has better antibacterial and cell compatibility than the control fiber. This suggests that the fabricated biocompatible scaffold is appropriate for a variety of wound dressing applications.

scholarly journals Silk Sericin Semi-interpenetrating Network Hydrogels Based on PEG-Diacrylate for Wound Healing Treatment

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Patchara Punyamoonwongsa ◽  
Supattra Klayya ◽  
Warayuth Sajomsang ◽  
Chanikarn Kunyanee ◽  
Sasitorn Aueviriyavit
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Sol Gel ◽  
Aqueous Media ◽  
Gelation Time ◽  
Dermal Fibroblasts ◽  
Interpenetrating Network ◽  
Silk Sericin ◽  
The Future

Silk sericin (SS) from the Bombyx mori silk cocoons has received much attention from biomedical scientists due to its outstanding properties, such as antioxidant, antibacterial, UV-resistant, and ability to release moisturizing factors. Unmodified SS does not self-assemble strongly enough to be used as a hydrogel wound dressing. Therefore, there is a need for suitable stabilization techniques to interlink the SS peptide chains or strengthen their structural cohesion. Here, we reported a method to form a silk semi-interpenetrating network (semi-IPN) structure through reacting with the short-chain poly(ethylene glycol) diacrylate (PEGDA) in the presence of a redox pair. Various hydrogels were prepared in aqueous media at the final SS/PEGDA weight percentages of 8/92, 15/85, and 20/80. Results indicated that all semi-IPN samples underwent a sol-gel transition within 70 min. The equilibrium water content (EWC) for all samples was found to be in the range of 70-80%, depending on the PEGDA content. Both the gelation time and the sol fraction decreased with the increased PEGDA content. This was due to the tightened network structure formed within the hydrogel matrices. Among all hydrogel samples, the 15/85 (SS/PEGDA) hydrogel displayed the maximum compressive strength (0.66 MPa) and strain (7.15%), higher than those of pure PEGDA. This implied a well-balanced molecular interaction within the SS/PEGDA/water systems. Based on the direct and indirect MTS assay, the 15/85 hydrogel showed excellent in vitro biocompatibility towards human dermal fibroblasts, representing a promising material for biomedical wound dressing in the future. A formation of a semi-IPN structure has thus proved to be one of the best strategies to extend a practical limit of using SS hydrogels for wound healing treatment or other biomedical hydrogel matrices in the future.

scholarly journals Fungal Colonization and Biodeterioration of Plasticized Polyvinyl Chloride

2000 ◽  
Vol 66 (8) ◽  
pp. 3194-3200 ◽  
Author(s):  
Jeremy S. Webb ◽  
Marianne Nixon ◽  
Ian M. Eastwood ◽  
Malcolm Greenhalgh ◽  
Geoffrey D. Robson ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Morphological Characteristics ◽  
Sole Source ◽  
Dioctyl Phthalate ◽  
Microbial Colonization ◽  
Fungal Colonization ◽  
In Vitro Tests ◽  
Rrna Gene ◽  
Microbial Succession

ABSTRACT Significant substratum damage can occur when plasticized PVC (pPVC) is colonized by microorganisms. We investigated microbial colonization of pPVC in an in situ, longitudinal study. Pieces of pPVC containing the plasticizers dioctyl phthalate and dioctyl adipate (DOA) were exposed to the atmosphere for up to 2 years. Fungal and bacterial populations were quantified, and colonizing fungi were identified by rRNA gene sequencing and morphological characteristics.Aureobasidium pullulans was the principal colonizing fungus, establishing itself on the pPVC between 25 and 40 weeks of exposure. A group of yeasts and yeast-like fungi, includingRhodotorula aurantiaca and Kluyveromyces spp., established themselves on the pPVC much later (after 80 weeks of exposure). Numerically, these organisms dominated A. pullulans after 95 weeks, with a mean viable count ± standard error of 1,000 ± 200 yeast CFU cm−2, compared to 390 ± 50 A. pullulans CFU cm−2. No bacterial colonization was observed. We also used in vitro tests to characterize the deteriogenic properties of fungi isolated from the pPVC. All strains of A. pullulans tested could grow with the intact pPVC formulation as the sole source of carbon, degrade the plasticizer DOA, produce extracellular esterase, and cause weight loss of the substratum during growth in vitro. In contrast, several yeast isolates could not grow on pPVC or degrade DOA. These results suggest that microbial succession may occur during the colonization of pPVC and that A. pullulans is critical to the establishment of a microbial community on pPVC.

Sign in / Sign up

Export Citation Format

Share Document