scholarly journals Natural Isotope Abundances of Carbon and Nitrogen in Tissue Proteins and Amino Acids as Biomarkers of the Decreased Carbohydrate Oxidation and Increased Amino Acid Oxidation Induced by Caloric Restriction under a Maintained Protein Intake in Obese Rats

Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1087 ◽  
Author(s):  
Jean-François Huneau ◽  
Olivier L. Mantha ◽  
Dominique Hermier ◽  
Véronique Mathé ◽  
Guillaume Galmiche ◽  
...  
Keyword(s):  
Amino Acids ◽  
Caloric Restriction ◽  
Protein Intake ◽  
Skeletal Muscles ◽  
Acid Oxidation ◽  
Protein Mass ◽  
Obese Rats ◽  
Lower Protein ◽  
Underlying Mechanisms ◽  
Δ15n And Δ13c

A growing body of evidence supports a role for tissue-to-diet 15N and 13C discrimination factors (Δ15N and Δ13C), as biomarkers of metabolic adaptations to nutritional stress, but the underlying mechanisms remain poorly understood. In obese rats fed ad libitum or subjected to gradual caloric restriction (CR), under a maintained protein intake, we measured Δ15N and Δ13C levels in tissue proteins and their constitutive amino acids (AA) and the expression of enzymes involved in the AA metabolism. CR was found to lower protein mass in the intestine, liver, heart and, to a lesser extent, some skeletal muscles. This was accompanied by Δ15N increases in urine and the protein of the liver and plasma, but Δ15N decreases in the proteins of the heart and the skeletal muscles, alongside Δ13C decreases in all tissue proteins. In Lys, Δ15N levels rose in the plasma, intestine, and some muscles, but fell in the heart, while in Ala, and to a lesser extent Glx and Asx, Δ13C levels fell in all these tissues. In the liver, CR was associated with an increase in the expression of genes involved in AA oxidation. During CR, the parallel rises of Δ15N in urine, liver, and plasma proteins reflected an increased AA catabolism occurring at the level of the liver metabolic branch point, while Δ15N decreases in cardiac and skeletal muscle proteins indicated increased protein and AA catabolism in these tissues. Thus, an increased protein and AA catabolism results in opposite Δ15N effects in splanchnic and muscular tissues. In addition, the Δ13C decrease in all tissue proteins, reflects a reduction in carbohydrate (CHO) oxidation and routing towards non-indispensable AA, to achieve fuel economy.

scholarly journals Acute Metabolic Response in Adults to Toddler Milk Formulas with Alternating Higher and Lower Protein and Fat Contents, a Randomized Cross-Over Trial

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3022
Author(s):  
Emily Newton-Tanzer ◽  
Hans Demmelmair ◽  
Jeannie Horak ◽  
Lesca Holdt ◽  
Berthold Koletzko ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Intake ◽  
Metabolic Response ◽  
Plasma Concentrations ◽  
Postprandial Metabolism ◽  
Postprandial Phase ◽  
Lower Protein ◽  
Branched Chain ◽  
Underlying Mechanisms ◽  
Time Specific

Protein intake in early life influences metabolism, weight gain, and later obesity risk. As such, a better understanding of the effects of protein intake on the postprandial metabolism and its dynamics over time may elucidate underlying mechanisms. In a randomized crossover study, we observed fasted adults who consumed two isocaloric toddler milk formulas concentrated as meals of 480 kcal with 67 g of carbohydrates 30 g (HP) or 7 g (LP) protein, and 10 g or 20 g fat, respectively. Anthropometry and body plethysmography were assessed, and blood samples collected at baseline and over five hours. Time-specific concentrations, areas under concentration curves (AUC), and maximum values of metabolites were compared by paired t-tests to examine the effects of protein content of toddler milks on postprandial plasma concentrations of insulin, glucose, branched-chain amino acids (BCAA), urea and triglycerides. Twenty-seven men and women aged 26.7 ± 5.0 years (BMI: 22.2 ± 2.5 kg/m2) (mean ± SD) participated. BCAA AUC, and Cmax values were significantly higher with HP than LP (144,765 ± 21,221 vs. 97,089 ± 14,650 µmol min/L, p < 0.001; 656 ± 120 vs. 407 ± 66 µmol/L, p < 0.001), as were insulin AUC and Cmax values (6674 ± 3013 vs. 5600 ± 2423 µmol min/L, p = 0.005; 71 ± 37 vs. 55 ± 28 µmol /L, p = 0.001). Higher glucose, urea, and triglyceride concentrations occurred in the late postprandial phase (≥180 min) with HP. In conclusion, we noted that higher milk protein intake induces increased postprandial BCAA concentrations for at least 5 h and led to higher initial insulin secretion. Gluconeogenesis due to an influx of amino acids and their degradation after HP meal might explain the late effects of protein intake on glucose and insulin.

Effect of protein intake on weight gain and plasma amino acid levels in uremic rats

1976 ◽  
Vol 230 (5) ◽  
pp. 1455-1459 ◽  
Author(s):  
M Wang ◽  
I Vyhmeister ◽  
JD Kopple ◽  
ME Swendseid
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Intake ◽  
Plasma Concentrations ◽  
Plasma Amino Acid ◽  
Uremic Patient ◽  
Lower Protein ◽  
Similar Weight ◽  
Amino Acid Levels ◽  
And Control

Chronically uremic rats weighing approximately 180-200 g and sham-operated controls of similar weight were pair fed diets containing 5, 15 or 23% protein for 10-12 wk. At each level of protein intake, uremic animals gained less weight and had lower protein efficiency ratios than controls. In addition, certain plasma amino acid levels were altered in the uremic animals. These included tyrosine and the tyrosine/phenylalanine ratio, which were decreased, and citrulline, glycine, and the methylhistidines, which were increased. In both uremic and control rats, plasma concentrations of certain amino acids, primarily nonessential ones, varied inversely with protein intake; with the 5% protein diet, the ratio of essential to nonessential amino acids was significantly reduced. These observations indicate that both uremia and reduced protein intake may affect growth and amino acid metabolism in rats with chronic renal failure. The finding that uremic rats utilize protein less efficiently may indicate that marked reductions in protein intake may be particularly hazardous to the nutritional status of the uremic patient.

scholarly journals Leptin-Mediated Changes in the Human Metabolome

2020 ◽  
Vol 105 (8) ◽  
pp. 2541-2552 ◽  
Author(s):  
Katherine Lawler ◽  
Isabel Huang-Doran ◽  
Takuhiro Sonoyama ◽  
Tinh-Hai Collet ◽  
Julia M Keogh ◽  
...  
Keyword(s):  
Amino Acids ◽  
Caloric Restriction ◽  
Severe Obesity ◽  
Caloric Intake ◽  
Branched Chain Amino Acids ◽  
Endocrine Function ◽  
Acid Oxidation ◽  
Obese People ◽  
Leptin Deficiency ◽  
Branched Chain

Abstract Context While severe obesity due to congenital leptin deficiency is rare, studies in patients before and after treatment with leptin can provide unique insights into the role that leptin plays in metabolic and endocrine function. Objective The aim of this study was to characterize changes in peripheral metabolism in people with congenital leptin deficiency undergoing leptin replacement therapy, and to investigate the extent to which these changes are explained by reduced caloric intake. Design Ultrahigh performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) was used to measure 661 metabolites in 6 severely obese people with congenital leptin deficiency before, and within 1 month after, treatment with recombinant leptin. Data were analyzed using unsupervised and hypothesis-driven computational approaches and compared with data from a study of acute caloric restriction in healthy volunteers. Results Leptin replacement was associated with class-wide increased levels of fatty acids and acylcarnitines and decreased phospholipids, consistent with enhanced lipolysis and fatty acid oxidation. Primary and secondary bile acids increased after leptin treatment. Comparable changes were observed after acute caloric restriction. Branched-chain amino acids and steroid metabolites decreased after leptin, but not after acute caloric restriction. Individuals with severe obesity due to leptin deficiency and other genetic obesity syndromes shared a metabolomic signature associated with increased BMI. Conclusion Leptin replacement was associated with changes in lipolysis and substrate utilization that were consistent with negative energy balance. However, leptin’s effects on branched-chain amino acids and steroid metabolites were independent of reduced caloric intake and require further exploration.

scholarly journals Severe anemia in patients with Propionic acidemia is associated with branched-chain amino acid imbalance

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Sinziana Stanescu ◽  
Amaya Belanger-Quintana ◽  
Borja Manuel Fernandez-Felix ◽  
Francisco Arrieta ◽  
Victor Quintero ◽  
...  
Keyword(s):  
Amino Acids ◽  
Food Consumption ◽  
Protein Intake ◽  
Propionic Acidemia ◽  
Study Data ◽  
Metabolic Decompensation ◽  
Severe Anemia ◽  
Medical Food ◽  
Natural Protein ◽  
Branched Chain

Abstract Background Propionic acidemia (PA), an inborn error of metabolism, is caused by a deficiency in propionyl-CoA carboxylase. Patients have to follow a diet restricted in the propiogenic amino acids isoleucine (Ile), valine (Val), methionine (Met) and threonine (Thr); proper adherence can prevent and treat acute decompensation and increase life expectancy. However, chronic complications occur in several organs even though metabolic control may be largely maintained. Bone marrow aplasia and anemia are among the more common. Materials and methods In this retrospective study, data for patients with PA being monitored at the Hospital Ramón y Cajal (Madrid, Spain) (n = 10) in the past 10 years were examined to statistically detect relationships between persistent severe anemia outside of metabolic decompensation episodes and dietary practices such as natural protein intake and medical food consumption (special mixture of precursor-free amino acids) along with plasma levels of branched-chain amino acids (BCAA). High ferritin levels were deemed to indicate that a patient had received repeated transfusions for persistent anemia since data on hemoglobin levels at the moment of transfusion were not always passed on by the attending centers. Results Three patients had severe, persistent anemia that required repeated blood transfusions. Higher medical food consumption and plasma Leu levels were associated with iron overload. Notably, natural protein intake and plasma Val were negatively correlated with ferritin levels. We also observed an inverse relationship between plasma Val/Leu and Ile/Leu ratios and ferritin. Conclusion The present results suggest that severe anemia in patients with PA might be associated with low natural protein intake and BCAA imbalance.

Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats

2021 ◽  
pp. 1-6
Author(s):  
Bruna Aléxia Cristofoletti Grillo ◽  
Lara C. Rocha ◽  
Giovana Z. Martinez ◽  
Jurandyr Pimentel Neto ◽  
Carolina dos Santos Jacob ◽  
...  
Keyword(s):  
Skeletal Muscles ◽  
Morphological Changes ◽  
Myotendinous Junction ◽  
Obese Rats

Abstract

scholarly journals Tracing the Trophic Plasticity of the Coral–Dinoflagellate Symbiosis Using Amino Acid Compound-Specific Stable Isotope Analysis

2021 ◽  
Vol 9 (1) ◽  
pp. 182
Author(s):  
Christine Ferrier-Pagès ◽  
Stephane Martinez ◽  
Renaud Grover ◽  
Jonathan Cybulski ◽  
Eli Shemesh ◽  
...  
Keyword(s):  
Amino Acids ◽  
Essential Role ◽  
Isotope Analysis ◽  
Diagnostic Tools ◽  
Global Changes ◽  
Biosynthetic Pathways ◽  
Trophic Plasticity ◽  
Trophic Index ◽  
Δ15n And Δ13c ◽  
Acid Compound

The association between corals and photosynthetic dinoflagellates is one of the most well-known nutritional symbioses, but nowadays it is threatened by global changes. Nutritional exchanges are critical to understanding the performance of this symbiosis under stress conditions. Here, compound-specific δ15N and δ13C values of amino acids (δ15NAA and δ13CAA) were assessed in autotrophic, mixotrophic and heterotrophic holobionts as diagnostic tools to follow nutritional interactions between the partners. Contrary to what was expected, heterotrophy was mainly traced through the δ15N of the symbiont’s amino acids (AAs), suggesting that symbionts directly profit from host heterotrophy. The trophic index (TP) ranged from 1.1 to 2.3 from autotrophic to heterotrophic symbionts. In addition, changes in TP across conditions were more significant in the symbionts than in the host. The similar δ13C-AAs signatures of host and symbionts further suggests that symbiont-derived photosynthates are the main source of carbon for AAs synthesis. Symbionts, therefore, appear to be a key component in the AAs biosynthetic pathways, and might, via this obligatory function, play an essential role in the capacity of corals to withstand environmental stress. These novel findings highlight important aspects of the nutritional exchanges in the coral–dinoflagellates symbiosis. In addition, they feature δ15NAA as a useful tool for studies regarding the nutritional exchanges within the coral–symbiodiniaceae symbiosis.

The effect of caloric restriction on working memory in healthy non-obese adults

CNS Spectrums ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 2-8 ◽  
Author(s):  
Emilie Leclerc ◽  
Alisson Paulino Trevizol ◽  
Ruth B. Grigolon ◽  
Mehala Subramaniapillai ◽  
Roger S. McIntyre ◽  
...  
Keyword(s):  
Working Memory ◽  
Energy Expenditure ◽  
Sleep Quality ◽  
Caloric Restriction ◽  
Neuropsychological Tests ◽  
Spatial Working Memory ◽  
Multicenter Trial ◽  
Obese Adults ◽  
Parallel Group ◽  
Lower Protein

Objective.We aim to evaluate the effect of caloric restriction (CR) in cognition by comparing performance in neuropsychological tests for working memory between a group of non-obese healthy subjects doing CR for 2 years with another consuming ad libitum diet (AL).Methods.This study was part of a larger multicenter trial called CALERIE that consisted of a randomized clinical trial with parallel-group comparing 2 years of 25% CR and AL in 220 volunteers with a BMI between 22 and 28 kg/m2, across 3 sites. The cognitive tests used were the Cambridge Neuropsychological Tests Automated Battery (CANTAB) for Spatial Working Memory (SWM) including the total number of errors (SWMTE) and strategy (SWMS). Included as possible moderators were sleep quality, mood states, perceived stress, and energy expenditure. Analyses were performed at baseline and months 12 and 24.Results.After adjustments, there was a significantly greater improvement in working memory assessed by the SWM for CR individuals, compared to AL. At month 24, it was related mostly to lower protein intake, compared to other macronutrients. Changes in SWM were moderated by changes in sleep quality, physical activity, and energy expenditure.Conclusion.On the long term, CR in healthy individuals seems to have a slightly positive effect on working memory. The study of brain CR targets opens new possibilities to prevent and treat cognitive deficits.

Lower Abdominal Fatness And Lower Protein Intake Are Risk Factors For Sarcopenic And Dynapenic Women

2016 ◽  
Vol 48 ◽  
pp. 145
Author(s):  
Leonardo E. Perera ◽  
Mariana S. Nakagaki ◽  
Edilaine Michelin ◽  
Gabriel N. Vilches ◽  
Okesley Teixeira ◽  
...  
Keyword(s):  
Risk Factors ◽  
Protein Intake ◽  
Lower Protein ◽  
Abdominal Fatness

Technology of production and quality assessment of direct-squeezed juice and concentrated elderberry growing in the Republic of Belarus

2021 ◽  
pp. 83-87
Author(s):  
Леонид Чеславович Бурак ◽  
Андрей Петрович Завалей
Keyword(s):  
Amino Acids ◽  
Physicochemical Parameters ◽  
Mass Fraction ◽  
Essential Amino Acids ◽  
Quantitative Composition ◽  
Protein Mass ◽  
Juice Extraction ◽  
The Republic ◽  
First Time ◽  
Technology Of Production

Авторами статьи впервые разработана технология производства соков прямого отжима и высокой концентрации из плодов бузины садовой и дикорастущей, собранных на территории Беларуси. Сок прямого отжима получали при помощи ферментации с последующей фильтрацией. Концентрированный сок был получен из сока прямого отжима и экстрагирования выжимок. Для максимального извлечения сока после дробления при ферментативной обработке была использована температура 50 °С в течение 240 мин, количество ферментного препарата составляло 400 см/т. Были исследованы органолептические и физико-химические показатели сока прямого отжима и сока концентрированного: массовая доля сухих веществ, aктивная кислотность (рН), массовая доля пектиновых веществ, cодержание антоцианов, белка, массовая доля органических кислот. Исследованы показатели безопасности продукции, впервые определен состав аминокислот (качество и количество) в концентрированном соке бузины. Установлено, что совокупность физико-химических показателей позволяет отнести сок прямого отжима из бузины к профилактическим напиткам. В состав сока бузины высокой концентрации входят 18 аминокислот в количестве 4,84 г/100 мл. Незаменимых аминокислот выявлено 7 в количестве 1,51 г/100 мл. Также сок суммарно содержит фенолы в соотношении 42,95 мг-экв галловой кислоты/г сухого вещества и большое количество гидролизованных танинов. При этом большинство полифенольных соединений избежали разрушения в процессе концентрации. The authors of the article developed for the first time a technological process for the production of direct-squeezed juice and concentrated juice from the fruits of garden and wild elderberry growing in the Republic of Belarus. Direct-squeezed juice was obtained by fermentation followed by filtration. Concentrated juice was obtained by processing direct-squeezed juice and extracting the marc. For maximum juice extraction after crushing during enzymatic treatment, a temperature of 50 °C was used for 240 min, the dose of the enzyme preparation was 400 cm/t. Direct-squeezed juice and concentrated juice were studied by organoleptic and physicochemical parameters: mass fraction of solids, active acidity (pH), mass fraction of pectin substances, content of anthocyanins, protein, mass fraction of organic acids. The product safety indicators were studied, the qualitative and quantitative composition of amino acids in concentrated elderberry juice was first determined. It has been established that by the combination of physicochemical parameters, direct squeezed juice from elderberry can be considered a preventive drink. The composition of concentrated elderberry juice includes 18 amino acids, in the amount of 4.84 g/100 ml. 7 essential amino acids were detected in the amount of 1.51 g/100 ml. The concentrated juice contained phenols in the quantity of 42.95 mEq gallic acid / gram dry matter and a large number of hydrolyzed tannins. Most polyphenolic compounds were preserved after the juice concentration.

Plasmalogens improve swimming performance by modulating the expression of genes involved in amino acids and lipid metabolism, oxidative stress, and ferroptosis in an Alzheimer's disease zebrafish model

2021 ◽  
Author(s):  
Junli Feng ◽  
Gongshuai Song ◽  
Yuanyuan Wu ◽  
Xi Chen ◽  
Jie Pang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Lipid Metabolism ◽  
Human Health ◽  
Swimming Performance ◽  
Cognitive Impairments ◽  
Zebrafish Model ◽  
Expression Of Genes ◽  
Health Studies ◽  
Underlying Mechanisms

Plasmalogens (PLs) are critical to human health. Studies have reported a link between downregulation of PLs levels and cognitive impairments in patients with Alzheimer´s disease (AD). however, the underlying mechanisms...

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