scholarly journals Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2606 ◽  
Author(s):  
Mercè Hereu ◽  
Sara Ramos-Romero ◽  
Roser Marín-Valls ◽  
Susana Amézqueta ◽  
Bernat Miralles-Pérez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Weight Gain ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Bacterial Strains ◽  
Pufa Supplementation ◽  
Sprague Dawley Rats ◽  
Chain Fatty Acids

Some functional food components may help maintain homeostasis by promoting balanced gut microbiota. Here, we explore the possible complementary effects of d-fagomine and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA 1:1) on putatively beneficial gut bacterial strains. Male Sprague–Dawley rats were supplemented with d-fagomine, ω-3 PUFAs, or both, for 23 weeks. Bacterial subgroups were evaluated in fecal DNA by quantitative real-time polymerase chain reaction (qRT-PCR) and short-chain fatty acids were determined by gas chromatography. We found that the populations of the genus Prevotella remained stable over time in animals supplemented with d-fagomine, independently of ω-3 PUFA supplementation. Animals in these groups gained less weight than controls and rats given only ω-3 PUFAs. d-Fagomine supplementation together with ω-3 PUFAs maintained the relative populations of Bacteroides. ω-3 PUFAs alone or combined with d-fagomine reduced the amount of acetic acid and total short-chain fatty acids in feces. The plasma levels of pro-inflammatory arachidonic acid derived metabolites, triglycerides and cholesterol were lower in both groups supplemented with ω-3 PUFAs. The d-fagomine and ω-3 PUFAs combination provided the functional benefits of each supplement. Notably, it helped stabilize populations of Prevotella in the rat intestinal tract while reducing weight gain and providing the anti-inflammatory and cardiovascular benefits of ω-3 PUFAs.

Short-chain fatty acids inhibit cAMP-mediated chloride secretion in rat colon

1996 ◽  
Vol 271 (6) ◽  
pp. C1853-C1860 ◽  
Author(s):  
P. C. Dagher ◽  
R. W. Egnor ◽  
A. Taglietta-Kohlbrecher ◽  
A. N. Charney
Keyword(s):  
Fatty Acids ◽  
Short Circuit ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Rat Colon ◽  
Sprague Dawley ◽  
T84 Cells ◽  
Ussing Chambers ◽  
Cl Secretion ◽  
Chain Fatty Acids

Butyrate stimulates salt absorption in mammalian colon. We examined whether butyrate also affects Cl- secretion. Mucosal segments of distal colon of male Sprague-Dawley rats and T84 cells were studied in Ussing chambers. In control colon, 1 mM dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) increased short-circuit current (Isc) and serosal-to-mucosal Cl- flux (JsmCl) by 3.2 +/- 0.8 and 2.9 +/- 0.8 mueq.cm-2.h-1, respectively. Mucosal or serosal 25 mM butyrate prevented DBcAMP-induced increases in Isc and JsmCl. Four and eight millimolar butyrate caused half-maximal inhibition of the increases in JsmCl and Isc, respectively. Butyrate also inhibited basal JsmCl (by 2.0 +/- 0.4 mueq.cm-2.h-1) but not carbachol-mediated Cl- secretion. The relative inhibitory potency at 25 mM of other short-chain fatty acids (SCFA) paralleled their degree of cellular metabolism: butyrate > acetate = propionate > isobutyrate. At 25 mM, all SCFA reduced mucosal intracellular pH (pHi) transiently by 0.1 pH unit. In intact T84 cells, 50 mM butyrate inhibited the DBcAMP-induced rise in Isc by 55%. In T84 cells with nystatin-permeabilized basolateral membranes, butyrate inhibited the increase in Isc by 82%. We conclude that butyrate inhibits basal and cAMP-mediated Cl- secretion by a mechanism independent of pHi, possibly located at the apical membrane.

scholarly journals Short chain fatty acids and methylamines produced by gut microbiota as mediators and markers in the circulatory system

2020 ◽  
Vol 245 (2) ◽  
pp. 166-175 ◽  
Author(s):  
Maksymilian Onyszkiewicz ◽  
Kinga Jaworska ◽  
Marcin Ufnal
Keyword(s):  
Fatty Acids ◽  
Cardiovascular Diseases ◽  
Gut Microbiota ◽  
Therapeutic Targets ◽  
Circulatory System ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Bacterial Strains ◽  
Ample Evidence ◽  
Chain Fatty Acids

Ample evidence suggests that gut microbiota-derived products affect the circulatory system functions. For instance, short chain fatty acids, that are the products of dietary fiber bacterial fermentation, have been found to dilate blood vessels and lower blood pressure. Trimethylamine, a gut bacteria metabolite of carnitine and choline, has recently emerged as a potentially toxic molecule for the circulatory system. To enter the bloodstream, microbiota products cross the gut–blood barrier, a multilayer system of the intestinal wall. Notably, experimental and clinical studies show that cardiovascular diseases may compromise function of the gut–blood barrier and increase gut-to-blood penetration of microbiota-derived molecules. Hence, the bacteria products and the gut–blood barrier may be potential diagnostic and therapeutic targets in cardiovascular diseases. In this paper, we review research on the cardiovascular effects of microbiota-produced short chain fatty acids and methylamines. Impact statement Despite a progress in the diagnosis and treatment of cardiovascular diseases, there are still significant gaps in understanding complex mechanisms underlying cardiovascular pathology. Increasing evidence suggests that gut microbiota products such as short chain fatty acids or methylamines may affect the circulatory system in health and disease. Hence, the microbiota-derived molecules are potential diagnostic and therapeutic targets in cardiovascular diseases. Therapeutic options may include administration of selected bacterial strains (probiotics) producing desired metabolites or administration of direct gut microbiota products.

scholarly journals Effect of Butyrylated Arrowroot Starch to the Digesta Profile and Molar Ratio SCFA

2013 ◽  
Vol 2 (2) ◽  
pp. 144
Author(s):  
Damat Damat
Keyword(s):  
Fatty Acids ◽  
Water Content ◽  
Butyric Acid ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Molar Ratio ◽  
Sprague Dawley ◽  
Randomized Design ◽  
Sprague Dawley Rats ◽  
Arrowroot Starch

The research was conducted to determine the effects of Butyrylated Arrowroot Starch (BAS) to the digesta profile and Short Chain Fatty Acids (SCFA) molar ratio. The research was conducted by using simple complete randomized design (CRD). The treatment tested was feed type, which consists of 5 levels, namely natural arrowroot starch, BAS with Degree of Substitution (DS) 0.053; 0.120; 0.187 and AIN93 natural feed. The study was conducted by using 30 Sprague Dawley rats, which were divided into 5 groups, each group consisting of 6 tails. Feeding treatments were given from dayseven until day 33. On day-34 analysis was conductedof the digesta profile and the SCFA molar ratio. Based on the analysis results, it is known that BAS feeding increased digesta weight, decreased digesta pH and increased digesta water content when compared with the AIN93 standard feed. Largest amount of digesta, lowest digesta pH, and highest water content of digesta, respectively 7.19 g, 6.53 and 91.23%, wasobtained from rats fed BAS with DS 0.187. The molar ratio of butyric acid in digesta also increased along with the increasing BAS DS.<strong><em></em></strong><em></em>

scholarly journals Considerations on goat milk biochemical composition

2021 ◽  
Vol 32 (1) ◽  
pp. 85-89
Author(s):  
Andreea Anghel ◽  
Daniela Jitariu ◽  
Dorina Nadolu ◽  
Zoia Zamfir ◽  
Elena Ilişiu
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Maximum Level ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Human Consumption ◽  
Omega 3 ◽  
Goat’S Milk ◽  
Goat's Milk ◽  
Chain Fatty Acids

Abstract The benefits of human consumption of goat's milk are given by the presence in this milk of short-chain fatty acids (approximately 20% are short-chain fatty acids) and medium-chain fatty acids (55%), this milk being easier to digest. An important qualitative indicator of goat's milk with technological, nutritional and dietary impact is the fat content. Our data show that the percentage of milk fat increases immediately after parturition, then decreases for most of the lactation. This is due to two factors: a diluting effect, by increasing the volume of milk to the maximum level of lactation and a decreasing effect of lipid mobilization, which leads to a decrease in the plasma level of unesterified fatty acids (especially C18:0 and C18:1), with a role in lipid synthesis in the mammary gland. From the third month of lactation, the average daily amount of milking milk undergoes only slight variations. Also, the fat and protein percentage remain relatively constant during June-August. In summer there was an increase in the levels of monounsaturated, polyunsaturated fatty acids and of conjugated linoleic acids in milk, compared to spring. Our results indicate that multiparous Carpathian breed females, whose food comes mostly from grazing, produce milk during the summer with a ratio between omega-6 and omega-3 below 4.

scholarly journals FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes

2021 ◽  
Vol 12 ◽  
Author(s):  
Bénédicte Colnet ◽  
Christian M. K. Sieber ◽  
Fanny Perraudeau ◽  
Marion Leclerc
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
False Positive Rate ◽  
Short Chain Fatty Acids ◽  
Glycoside Hydrolases ◽  
Short Chain ◽  
Bacterial Strains ◽  
Positive Rate ◽  
Chain Fatty Acids

Dietary fibers impact gut colonic health, through the production of short-chain fatty acids. A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens. Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids. However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity. Our goal was to explore how the capacity of strains, from the Bacteroidetes phylum, to grow on fiber could be predicted from their genome sequences. We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains. To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data. FiberGrowth’s prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41). Being able to better predict fiber breakdown by bacterial strains would help to understand their impact on human nutrition and health. Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.

scholarly journals Influence of starches of low digestibility on the rat caecal microflora

1988 ◽  
Vol 60 (3) ◽  
pp. 597-604 ◽  
Author(s):  
A. K. Mallett ◽  
C. A. Bearne ◽  
P. J. Young ◽  
I. R. Rowland ◽  
C. Berry
Keyword(s):  
Fatty Acids ◽  
Potato Starch ◽  
Intestinal Flora ◽  
Short Chain Fatty Acids ◽  
Ammonia Concentration ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Hind Gut ◽  
Branched Chain ◽  
Chain Fatty Acids

1. Male Sprague-Dawley rats were fed on either a purified, fibre-free diet or a diet in which half the maize starch was replaced with uncooked amylomaize or potato starch (equivalent to 100 or 200 g amylase-resistant starch (ARS)/kg diet respectively). Changes in short-chain fatty acids (SCFA), pH, ammonia and a number of bacterial variables in caecal contents were then assessed.2. Both ARS supplements decreased caecal content pH by approximately 1–2 units, with an associated reduction in ammonia concentration. Potato starch significantly decreased the concentration of SCFA in the hind-gut, while amylomaize supplementation increased propionic and butyric acids but decreased the occurrence of minor, branched-chain fatty acids.3. Caecal bacterial biotransformation activities (β-glucosidase (EC3.2.1.21), β-glucuronidase (EC3.2.1.31), reduction ofp-nitrobenzoic acid, apparent ammonia formation) were consistently decreased by both ARS sources.4. The results demonstrate that amylase-resistant carbohydrate altered toxicologically important functions in the large-intestinal flora of the rat.

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