scholarly journals PipeNig®-FL, a Fluid Extract of Black Pepper (Piper Nigrum L.) with a High Standardized Content of Trans-β-Caryophyllene, Reduces Lipid Accumulation in 3T3-L1 Preadipocytes and Improves Glucose Uptake in C2C12 Myotubes

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2788 ◽  
Author(s):  
Federica Geddo ◽  
Rosaria Scandiffio ◽  
Susanna Antoniotti ◽  
Erika Cottone ◽  
Giulia Querio ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Glucose Uptake ◽  
Lipid Accumulation ◽  
Cannabinoid Receptor ◽  
Adipogenic Differentiation ◽  
Black Pepper ◽  
Metabolic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
C2c12 Myotubes

Trans-β-caryophyllene (BCP) is a natural sesquiterpene hydrocarbon with several important pharmacological activities, including antioxidant, anti-inflammatory, anticancer, and cardioprotective functions. These properties are mainly due to its selective interaction with the peripherally expressed cannabinoid receptor 2. In addition, BCP activates peroxisome proliferated activator receptors α and γ and inhibits the Toll-like receptor signaling pathway. Given the growing scientific interest in BCP, the aim of our study was to investigate the metabolic effects of a black pepper extract (PipeNig®-FL), containing a high standardized content of BCP. In particular our interest was focused on its potential activity on lipid accumulation and glucose uptake. The extract PipeNig®-FL was chemically characterized by gas chromatography–mass spectrometry (GC–MS) and gas chromatography with flame-ionization detection (GC–FID), confirming a high content (814 mg/g) of BCP. Experiments were performed on 3T3-L1 preadipocytes and on C2C12 myotubes. Lipid content following 3T3-L1 adipogenic differentiation was quantified with AdipoRed fluorescence staining. Glucose uptake and GLUT4 membrane translocation were studied in C2C12 myotubes with the fluorescent glucose analog 2-NBDG and by immunofluorescence analysis. Here we show that PipeNig®-FL reduces 3T3-L1 adipocyte differentiation and lipid accumulation. Moreover, acute exposure of C2C12 myotubes to PipeNig®-FL improves glucose uptake activity and GLUT4 migration. Taken together, these results reveal interesting and novel properties of BCP, suggesting potential applications in the prevention of lipid accumulation and in the improvement of glucose uptake.

Comparison of Volatile Compounds in Pepper (Piper nigrum L.) by Simultaneous Distillation Extraction (SDE) and GC-MS

2011 ◽  
Vol 236-238 ◽  
pp. 2643-2646 ◽  
Author(s):  
Wen Xue Chen ◽  
Hai Gang Dou ◽  
Chang Ge ◽  
Cong Fa Li
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Volatile Compounds ◽  
Black Pepper ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Data Sets ◽  
White Pepper ◽  
Chromatography Mass Spectrometry ◽  
Simultaneous Distillation Extraction

In this study, the volatile compounds of pepper (Piper Nigrum L.) were extracted and analyzed by simultaneous distillation-extraction (SDE) and gas chromatography-mass spectrometry (GC-MS). The differences in volatile compounds were observed by GC-MS data sets. Results showed vinyl compounds were the most dominant volatiles in pepper by simultaneous distillation-extraction (SDE) and GC-MS, and 22 volatile compounds in the white pepper and black pepper were the same.

scholarly journals Secondary Metabolic Profile as a Tool for Distinction and Characterization of Cultivars of Black Pepper (Piper nigrum L.) Cultivated in Pará State, Brazil

2021 ◽  
Vol 22 (2) ◽  
pp. 890
Author(s):  
Luccas M. Barata ◽  
Eloísa H. Andrade ◽  
Alessandra R. Ramos ◽  
Oriel F. de Lemos ◽  
William N. Setzer ◽  
...  
Keyword(s):  
Black Pepper ◽  
Plant Diseases ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Volatile Components ◽  
Chemical Compositions ◽  
Multivariate Statistical ◽  
Protein Targets ◽  
Fruit Samples ◽  
Sesquiterpene Hydrocarbons

This study evaluated the chemical compositions of the leaves and fruits of eight black pepper cultivars cultivated in Pará State (Amazon, Brazil). Hydrodistillation and gas chromatography–mass spectrometry were employed to extract and analyze the volatile compounds, respectively. Sesquiterpene hydrocarbons were predominant (58.5–90.9%) in the cultivars “Cingapura”, “Equador”, “Guajarina”, “Iaçará”, and “Kottanadan”, and “Bragantina”, “Clonada”, and “Uthirankota” displayed oxygenated sesquiterpenoids (50.6–75.0%). The multivariate statistical analysis applied using volatile composition grouped the samples into four groups: γ-Elemene, curzerene, and δ-elemene (“Equador”/“Guajarina”, I); δ-elemene (“Iaçará”/“Kottanadan”/“Cingapura”, II); elemol (“Clonada”/“Uthirankota”, III) and α-muurolol, bicyclogermacrene, and cubebol (“Bragantina”, IV). The major compounds in all fruit samples were monoterpene hydrocarbons such as α-pinene, β-pinene, and limonene. Among the cultivar leaves, phenolics content (44.75–140.53 mg GAE·g−1 FW), the enzymatic activity of phenylalanine-ammonia lyase (20.19–57.22 µU·mL−1), and carotenoids (0.21–2.31 µg·mL−1) displayed significant variations. Due to black pepper’s susceptibility to Fusarium infection, a molecular docking analysis was carried out on Fusarium protein targets using each cultivar’s volatile components. F. oxysporum endoglucanase was identified as the preferential protein target of the compounds. These results can be used to identify chemical markers related to the susceptibility degree of black pepper cultivars to plant diseases prevalent in Pará State.

scholarly journals Comprehensive Two-Dimensional Gas Chromatography–Mass Spectrometry Analysis of Exhaled Breath Compounds after Whole Grain Diets

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2667
Author(s):  
Kaisa Raninen ◽  
Ringa Nenonen ◽  
Elina Järvelä-Reijonen ◽  
Kaisa Poutanen ◽  
Hannu Mykkänen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Metabolic Effects ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Wheat Bread ◽  
Exhaled Breath ◽  
Two Dimensional ◽  
Whole Grain ◽  
Chromatography Mass Spectrometry

Exhaled breath is a potential noninvasive matrix to give new information about metabolic effects of diets. In this pilot study, non-targeted analysis of exhaled breath volatile organic compounds (VOCs) was made by comprehensive two-dimensional gas chromatography–mass spectrometry (GCxGC-MS) to explore compounds relating to whole grain (WG) diets. Nine healthy subjects participated in the dietary intervention with parallel crossover design, consisting of two high-fiber diets containing whole grain rye bread (WGR) or whole grain wheat bread (WGW) and 1-week control diets with refined wheat bread (WW) before both diet periods. Large interindividual differences were detected in the VOC composition. About 260 VOCs were detected from exhaled breath samples, in which 40 of the compounds were present in more than half of the samples. Various derivatives of benzoic acid and phenolic compounds, as well as some furanones existed in exhaled breath samples only after the WG diets, making them interesting compounds to study further.

scholarly journals Volatiles of Black Pepper Fruits (Piper nigrum L.)

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4244 ◽  
Author(s):  
Dosoky ◽  
Satyal ◽  
Barata ◽  
da Silva ◽  
Setzer
Keyword(s):  
Essential Oils ◽  
Biological Activities ◽  
Herbal Medicines ◽  
Coastal Region ◽  
Hierarchical Cluster ◽  
Black Pepper ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Tropical Regions ◽  
Geographical Regions

Black pepper (Piper nigrum) is historically one of the most important spices and herbal medicines, and is now cultivated in tropical regions worldwide. The essential oil of black pepper fruits has shown a myriad of biological activities and is a commercially important commodity. In this work, five black pepper essential oils from eastern coastal region of Madagascar and six black pepper essential oils from the Amazon region of Brazil were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components of the essential oils were α-pinene, sabinene, β-pinene, δ-3-carene, limonene, and β-caryophyllene. A comparison of the Madagascar and Brazilian essential oils with black pepper essential oils from various geographical regions reported in the literature was carried out. A hierarchical cluster analysis using the data obtained in this study and those reported in the literature revealed four clearly defined clusters based on the relative concentrations of the major components.

scholarly journals α-Mangostin Improves Glucose Uptake and Inhibits Adipocytes Differentiation in 3T3-L1 Cells via PPARγ, GLUT4, and Leptin Expressions

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Muhammad Taher ◽  
Mohamed Zaffar Ali Mohamed Amiroudine ◽  
Tengku Muhamad Faris Syafiq Tengku Zakaria ◽  
Deny Susanti ◽  
Solachuddin J. A. Ichwan ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Lipid Accumulation ◽  
Adipogenic Differentiation ◽  
Stem Bark ◽  
Pcr Analysis ◽  
Effective Management ◽  
Fatty Acid Release ◽  
Uptake Activity ◽  
Acid Release

Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity ofα-mangostin, a major xanthone component isolated from the stem bark ofG. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPARγ, GLUT4, and leptin expressions.α-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 μM ofα-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed thatα-mangostin significantly improved the glucose uptake (P<0.05) with highest activity found at 25 μM. In addition,α-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 μM ofα-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition ofPPARγgene expression. Induction of glucose uptake and free fatty acid release byα-mangostin were accompanied by increasing mRNA expression ofGLUT4andleptin. These evidences propose thatα-mangostin might be possible candidate for the effective management of obesity in future.

scholarly journals The Study on Extraction Process and Analysis of Components in Essential Oils of Black Pepper (Piper nigrum L.) Seeds Harvested in Gia Lai Province, Vietnam

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 56 ◽  
Author(s):  
Thien Hien Tran ◽  
Le Ke Ha ◽  
Duy Chinh Nguyen ◽  
Tan Phat Dao ◽  
Le Thi Hong Nhan ◽  
...  
Keyword(s):  
Essential Oil ◽  
Respiratory Infections ◽  
Mesh Size ◽  
Extraction Process ◽  
Black Pepper ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Efficient Technology ◽  
Preservation Method

Black pepper (Piper nigrum L.) is a tropical crop with extensive medicinal potential in ethnomedicine and nutraceutical applications. The essential oil of black pepper finds wide applications in inhabitation of respiratory infections and soothing of muscular pains due to its warming and energizing property. The pungent bioactive piperine is responsible for this function, and therefore, efficient technology is required for an optimal extraction process of this compound. In the present article, we have developed a procedure for extracting black pepper essential oil from Vietnam, optimizing conditions that affect the extraction process. The effect of process parameters, namely material size, preservation method, the concentration of sodium chloride, the concentration of soak time, the ratio of material to water, temperature extraction, time extraction on the extraction yield, and relative efficiency were investigated. Results demonstrated that 20 g of black pepper milled with a mesh size of 160 obtained 0.48 g of essential oil (2.4%) at a raw material to water ratio of 1/21 (g/mL) at 150 °C in a time of 5.2 h. GC-MS (Gas chromatography–mass spectrometry) spectra showed that 3-carene (29.21%), D-limonene (20.94%), caryophyllene (15.05%), and β-pinene (9.77%) were present as major components. These results suggested that the essential oil extracted from Vietnamese black pepper is applicable in the manufacturing processes of insecticides and air deodorizers.

scholarly journals Optimization of Essential Oil Extraction Process of White Pepper (Piper nigrum L.) Harvested in Phu Quoc Island, Kien Giang Province, Vietnam

2020 ◽  
Vol 32 (11) ◽  
pp. 2707-2712
Author(s):  
Thien Hien Tran ◽  
Thi Cam Quyen Ngo ◽  
Hoang Duy Ngo ◽  
Nguyen Huu Thuan Anh ◽  
Ton Nu Thuy An ◽  
...  
Keyword(s):  
Essential Oil ◽  
Extraction Process ◽  
Black Pepper ◽  
Oil Extraction ◽  
Extraction Time ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Extraction Temperature ◽  
Green Pepper ◽  
White Pepper

Vietnam is a leading producer and exporter of products harvested from plants of the family Piperaceae such as green pepper, black pepper, red pepper and white pepper. In this study, an attempt is made to examine the essential oil extraction process with the material of white pepper grown and harvested in Phu Quoc island, Vietnam. To maximize essential oil production, three factors consisting the ratio of materials and solvents, extraction time and extraction temperature were selected and optimized. The surface response methodology optimization resulted the highest yield of 3.6%, achieved at the ratio of material and water ratio of 1:20 g/g, extraction time of 96 min and at 130 ºC. A high F values, low P values (< 0.0001), the determination coefficient (R2 = 0.9993) and a non-significant lack of fit suggested a strong correlation between actual and predicted values of the responses. The essential oil obtained was determined for chemical composition by gas chromatography-mass spectrometry (GC-MS) method. The GC-MS results showed that major constituents existing in the oil sample were limonene, 3-carene, sabinene, β-pinene, α-pinene and α-phellandrene, accounting for 27.059, 23.345, 17.903, 9.996, 5.167 and 4.588%, respectively.

scholarly journals Essential Oils from Fresh Fruits, Fruit Stalks and Stem Barks of Four Piper nigrum Varieties from Sarawak

1970 ◽  
Vol 3 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Irna Syairina Sahari ◽  
Zaini Assim ◽  
Fasihuddin Badruddin Ahmad ◽  
Ismail Bin Jusoh
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Principal Component ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Chemical Compositions ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Oil Components ◽  
Type Apparatus

The variation of chemical compositions in essential oils obtained from fresh fruits, fruit stalks and stem barks offour Piper nigrum L. varieties was analysed using capillary gas chromatography-flame ionization detector (GCFID)and gas chromatography-mass spectrometry (GC-MS). The essential oils were extracted byhydrodistillation Clevenger-type apparatus. The major oil constituents identified in all samples were β-elemene(1.07-2.93%), (+)-δ-cadinene (0.58-6.20%), α-caryophyllene (1.90-6.21%), δ-elemene (0.38-13.26%), 3-carene(0.07-16.82%) and β-caryophyllene (11.78-38.33%). All oil samples showed high content of sesquiterpenes,whereas fruit oils from Semongok Emas and Semongok Wan yielded mostly monoterpenes. Hierarchicalclustering and principal component analysis (PCA) of the oil components revealed significant correlationamongst individual P. nigrum oil samples.

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