scholarly journals Carbohydrate-Induced Insulin Signaling Activates Focal Adhesion Kinase: A Nutrient and Mechanotransduction Crossroads

Nutrients ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 3145 ◽  
Author(s):  
Dylan T. Wilburn ◽  
Steven B. Machek ◽  
Thomas D. Cardaci ◽  
Darryn S. Willoughby
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Insulin Signaling ◽  
Repeated Measures ◽  
Vastus Lateralis ◽  
Future Research ◽  
Placebo Condition ◽  
Carbohydrate Ingestion ◽  
Post Exercise ◽  
Repeated Measures Design

Research has suggested that nutrient, exercise, and metabolism-related proteins interact to regulate mammalian target of rapamycin complex one (mTOR) post-exercise and their interactions needs clarification. In a double-blind, cross-over, repeated measures design, ten participants completed four sets to failure at 70% of 1-repitition maximum (1-RM) with 45 s rest on angled leg press with or without pre-exercise maltodextrin (2 g/kg) after a 3 h fast. Vastus lateralis biopsies were collected at baseline before supplementation and 1 h post-exercise to analyze Focal Adhesion Kinase (FAK), ribosomal protein S6 kinase beta-1 (p70S6K), insulin receptor substrate 1 (IRS-1), phosphatidylinositol 3-kinase (PI3K), and 5′ AMP-activated protein kinase (AMPK) activation. FAK and IRS-1 activity were only elevated 1 h post-exercise with carbohydrate ingestion (p < 0.05). PI3K and p70S6K activation were both elevated after exercise in both conditions (p < 0.05). However, AMPK activity did not change from baseline in both conditions (p > 0.05). We conclude that FAK does not induce mTOR activation through PI3K crosstalk in response to exercise alone. In addition, FAK may not be regulated by AMPK catalytic activity, but this needs further research. Interestingly, carbohydrate-induced insulin signaling appears to activate FAK at the level of IRS-1 but did not enhance mTOR activity 1 h post-exercise greater than the placebo condition. Future research should investigate these interactions under different conditions and within different time frames to clearly understand the interactions between these signaling molecules.

Power of Modified Brown-Forsythe and Mixed-Model Approaches in Split-Plot Designs

Methodology ◽  
2017 ◽  
Vol 13 (1) ◽  
pp. 9-22 ◽  
Author(s):  
Pablo Livacic-Rojas ◽  
Guillermo Vallejo ◽  
Paula Fernández ◽  
Ellián Tuero-Herrero
Keyword(s):  
Repeated Measures ◽  
Statistical Power ◽  
Mixed Model ◽  
Covariance Structure ◽  
Simulation Method ◽  
Future Research ◽  
Repeated Measures Design ◽  
Fixed And Random Effects ◽  
Split Plot ◽  
High Level

Abstract. Low precision of the inferences of data analyzed with univariate or multivariate models of the Analysis of Variance (ANOVA) in repeated-measures design is associated to the absence of normality distribution of data, nonspherical covariance structures and free variation of the variance and covariance, the lack of knowledge of the error structure underlying the data, and the wrong choice of covariance structure from different selectors. In this study, levels of statistical power presented the Modified Brown Forsythe (MBF) and two procedures with the Mixed-Model Approaches (the Akaike’s Criterion, the Correctly Identified Model [CIM]) are compared. The data were analyzed using Monte Carlo simulation method with the statistical package SAS 9.2, a split-plot design, and considering six manipulated variables. The results show that the procedures exhibit high statistical power levels for within and interactional effects, and moderate and low levels for the between-groups effects under the different conditions analyzed. For the latter, only the Modified Brown Forsythe shows high level of power mainly for groups with 30 cases and Unstructured (UN) and Autoregressive Heterogeneity (ARH) matrices. For this reason, we recommend using this procedure since it exhibits higher levels of power for all effects and does not require a matrix type that underlies the structure of the data. Future research needs to be done in order to compare the power with corrected selectors using single-level and multilevel designs for fixed and random effects.

scholarly journals In Vivo Role of Focal Adhesion Kinase in Regulating Pancreatic  -Cell Mass and Function Through Insulin Signaling, Actin Dynamics, and Granule Trafficking

Diabetes ◽  
2012 ◽  
Vol 61 (7) ◽  
pp. 1708-1718 ◽  
Author(s):  
E. P. Cai ◽  
M. Casimir ◽  
S. A. Schroer ◽  
C. T. Luk ◽  
S. Y. Shi ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Insulin Signaling ◽  
Cell Mass ◽  
Actin Dynamics ◽  
Pancreatic Cell ◽  
And Function

scholarly journals THIRST RESPONSE TO POST-EXERCISE FLUID REPLACEMENT NEEDS AND CONTROLLED DRINKING

2016 ◽  
Vol 14 (2) ◽  
pp. 1-16
Author(s):  
Catalina Capitán-Jiménez ◽  
Luis Fernando Aragón-Vargas
Keyword(s):  
Plasma Volume ◽  
Repeated Measures ◽  
Fluid Replacement ◽  
Water Volume ◽  
Volume Changes ◽  
Physically Active ◽  
Post Exercise ◽  
Repeated Measures Design ◽  
Controlled Drinking ◽  
Before And After

Perceived thirst (TP) was evaluated as a dependent variable: can it distinguish among several levels of acute dehydration, is it reliable, and how does it respond to the ingestion of a fixed water volume post exercise? In a repeated-measures design, eight physically active students (24.5±3.6 years, mean±SD), reported to the laboratory on four non-consecutive days. They remained at rest or exercised at 32±3°C db and 65±6% rh to a randomly assigned dehydration equivalent to 1, 2, and 3% of body mass (BM). Following exercise, participants ingested a fixed water volume of 1.20% BM in 30 minutes; urine output, TP and plasma volume changes were assessed every 30 minutes over 3 hours. Post-exercise TP was not different before and after showering (p = 0.860), but it was significantly different among conditions (TP = 2.50 ± 0.45, 4.44 ± 0.72, 6.38 ± 0.82, and 8.63 ± 0.18 for 0, 1, 2, and 3% BM, p = 0.001). TP was associated with net fluid balance (rpart = -0.62, p < 0.0001) but, soon after drinking, TP was the same regardless of dehydration (p>0.05). Thirst perception is valid and reliable in the absence of drinking but it responds inappropriately to water intake.

scholarly journals Investigating Care Dependency and Its Relation to Outcome (ICARE): Results From a Naturalistic Study of an Intensive Day Treatment Program for Depression

2021 ◽  
Vol 12 ◽  
Author(s):  
Sarah Glanert ◽  
Svenja Sürig ◽  
Ulrike Grave ◽  
Eva Fassbinder ◽  
Sebastian Schwab ◽  
...  
Keyword(s):  
Treatment Outcome ◽  
Depressive Symptoms ◽  
Repeated Measures ◽  
Mixed Model ◽  
Depressive Symptomatology ◽  
Future Research ◽  
Clinical Trial Registration ◽  
Levels Of Care ◽  
Repeated Measures Design ◽  
Care Dependency

Background: This study explores the association of experienced dependency in psychotherapy as measured with the CDQ (Care Dependency Questionnaire) and treatment outcome in depression. Furthermore, the course of care dependency and differences in the CDQ scores depending on the received type of treatment, MCT (metacognitive therapy), or CBASP (cognitive behavioral analysis system of psychotherapy), were investigated.Methods: The study follows a prospective, parallel group observational design. Patients suffering from depression received an 8-week intensive day clinic program, which was either CBASP or MCT. The treatment decision was made by clinicians based on the presented symptomatology and with regard to the patients' preferences. The patients reported depressive symptoms with the QIDS-SR16 (Quick Inventory of Depressive Symptomatology) and levels of experienced care dependency with the German version of the CDQ on a weekly basis. Mixed-model analyses were run to account for the repeated-measures design.Results: One hundred patients were included in the analyses. Results indicate that higher levels of care dependency might predict a less favorable outcome of depressive symptomatology. Levels of care dependency as well as depressive symptoms decreased significantly over the course of treatment. There was no significant between-group difference in care dependency between the two treatment groups.Conclusion: The results suggest that care dependency might be associated with a worse treatment outcome in depressed patients. In general, care dependency seems to be a dynamic construct, as it is changing over time, while the levels of care dependency seem to be independent from the received type of treatment. Future research should continue investigating the mechanisms of care dependency in a randomized controlled design.Clinical Trial Registration:https://www.drks.de/drks_web/, identifier: DRKS00023779.

Reduced carbohydrate availability enhances exercise-induced p53 signaling in human skeletal muscle: implications for mitochondrial biogenesis

2013 ◽  
Vol 304 (6) ◽  
pp. R450-R458 ◽  
Author(s):  
Jonathan D. Bartlett ◽  
Jari Louhelainen ◽  
Zafar Iqbal ◽  
Andrew J. Cochran ◽  
Martin J. Gibala ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Biogenesis ◽  
Repeated Measures ◽  
Human Skeletal Muscle ◽  
Vastus Lateralis ◽  
Oxidative Capacity ◽  
Pyruvate Dehydrogenase Kinase ◽  
Repeated Measures Design ◽  
P53 Signaling ◽  
Exercise Induced

The mechanisms that regulate the enhanced skeletal muscle oxidative capacity observed when training with reduced carbohydrate (CHO) availability are currently unknown. The aim of the present study was to test the hypothesis that reduced CHO availability enhances p53 signaling and expression of genes associated with regulation of mitochondrial biogenesis and substrate utilization in human skeletal muscle. In a repeated-measures design, muscle biopsies (vastus lateralis) were obtained from eight active males before and after performing an acute bout of high-intensity interval running with either high (HIGH) or low CHO availability (LOW). Resting muscle glycogen (HIGH, 467 ± 19; LOW, 103 ± 9 mmol/kg dry wt) was greater in HIGH compared with LOW ( P < 0.05). Phosphorylation (P-) of ACCSer79 (HIGH, 1.4 ± 0.4; LOW, 2.9 ± 0.9) and p53Ser15 (HIGH, 0.9 ± 0.4; LOW, 2.6 ± 0.8) was higher in LOW immediately postexercise and 3 h postexercise, respectively ( P < 0.05). Before and 3 h postexercise, mRNA content of pyruvate dehydrogenase kinase 4, mitochondrial transcription factor A, cytochrome- c oxidase IV, and PGC-1α were greater in LOW compared with HIGH ( P < 0.05), whereas carnitine palmitoyltransferase-1 showed a trend toward significance ( P = 0.09). However, only PGC-1α expression was increased by exercise ( P < 0.05), where three-fold increases occurred independently of CHO availability. We conclude that the exercise-induced increase in p53 phosphorylation is enhanced in conditions of reduced CHO availability, which may be related to upstream signaling through AMPK. Given the emergence of p53 as a molecular regulator of mitochondrial biogenesis, such nutritional modulation of contraction-induced p53 activation has implications for both athletic and clinical populations.

scholarly journals Investigating Parietal and Premotor Influence on Motor Cortical Excitability Associated with Visuomotor Associative Plasticity

2021 ◽  
Vol 11 (4) ◽  
pp. 452
Author(s):  
Paul J. Wolfe ◽  
Lynea B. Kaethler ◽  
W. Richard Staines
Keyword(s):  
Repeated Measures ◽  
Cortical Excitability ◽  
Occipital Cortex ◽  
Long Term Potentiation ◽  
Future Research ◽  
Reaching Movement ◽  
Repeated Measures Design ◽  
Term Potentiation ◽  
Motor Cortex Excitability

The brain changes in response to sensory signals it is exposed to. It has been shown that long term potentiation-like neuroplasticity can be experimentally induced via visual paired-associative stimulation (V-PAS). V-PAS combines afferent visual stimuli with a transcranial magnetic stimulation pulse to induce plasticity. Preparation of a reaching movement to generate activity in superior parietal occipital cortex (SPOC) was used in this study as an additional afferent contributor to modulate the resultant plasticity. We hypothesized that V-PAS with a reaching movement would induce greater cortical excitability than V-PAS alone and would exhibit facilitated SPOC to M1 projections. All four experiments enrolled groups of 10 participants to complete variations of V-PAS in a repeated measures design. SPOC to M1 projections facilitated motor cortex excitability following V-PAS regardless of intervention received. We did not observe evidence indicating extra afferent information provided an additive effect to participants. Investigation of PMd to M1 projections confirmed disinhibition and suggested interneuronal populations within M1 may be mechanistically involved. Future research should look to rule out the existence of an upper limit for effective afference during V-PAS and investigate the average influence of V-PAS on cortical excitability in the larger population.

scholarly journals Self-Confidence and Perceptions of Athletic Training Students Following Simulated Experiences: A Mixed-Methods Pilot Study

2021 ◽  
Author(s):  
Hannah Stedge ◽  
Valerie Herzog
Keyword(s):  
Mixed Methods ◽  
Athletic Training ◽  
Clinical Education ◽  
Repeated Measures ◽  
Real Life ◽  
Future Research ◽  
High Fidelity ◽  
Athletic Training Students ◽  
Repeated Measures Design ◽  
Self Confidence

Purpose: Athletic training clinical education provides students with a variety of real-life patient encounters under the supervision of a credentialed athletic trainer. However, clinical education experiences may not allow for all students to practice more invasive or less common skills such as rectal thermometry. The purpose of this study was two-fold: 1) determine the effect of a series of low to high-fidelity simulated rectal thermometry experiences on athletic training students’ self-confidence scores; and 2) through a phenomenological qualitative approach, explore athletic training students’ perceptions following the simulated rectal thermometry experiences. Method: This study used a quasi-experimental, mixed methods, one-group repeated measures design. Participants were nine first year professional Master of Athletic Training students who completed four simulated experiences on the skill of rectal thermometry. The outcome measures were The Athletic Trainer’s Self-Confidence Scale (ATSCS) and qualitative semi-structured open-ended interviews. Results: Friedman’s ANOVA revealed a statistically significant improvement from the participant’s baseline ATSCS scores to the last post-test scores. We identified three major themes following qualitative interviews: 1) Perception changes before and after participation; 2) Benefits of a series of four simulations; 3) Explanations for the improvements in confidence and competence. Conclusions: A series of simulated learning activities using high-fidelity and standardized patient interactions can help improve athletic training students’ self-confidence. Though future research is needed, athletic training education programs should consider implementing simulated experiences to equip athletic training students to transition to practice.

Positively Engaged Pathways: A Brief Pre-Employment Intervention for Transition Age Youth With Disabilities

2020 ◽  
Vol 34 (4) ◽  
pp. 284-296
Author(s):  
Allison R. Fleming ◽  
Brian N. Phillips ◽  
Elizabeth H. Gilfillan ◽  
Joy Gray ◽  
Megan McCloskey
Keyword(s):  
Repeated Measures ◽  
School Setting ◽  
Future Research ◽  
Transition Age Youth ◽  
Youth With Disabilities ◽  
Transition Age ◽  
Postsecondary Outcomes ◽  
Repeated Measures Design ◽  
Counseling Interventions ◽  
The Relationship

PurposeThis study was a pilot of a brief psychological capital (PsyCap) intervention with a sample of transition age youth with disabilities (N = 24) in a school setting. Substantial evidence supports the relationship between PsyCap and employment outcomes. Transition age youth with disabilities are an important target for pre-employment counseling interventions due to a lack of parity in postsecondary outcomes. Researchers have successfully boosted PsyCap in adult samples using brief interventions.MethodsPsyCap and vocational outcome expectancy (VOE) were compared in a repeated measures design.ResultsPsyCap and VOE, and PsyCap and school performance were significantly correlated in our sample, but no differences were observed in PsyCap or VOE postintervention.ConclusionAs the first known effort to develop a PsyCap driven pre-employment intervention for transition age youth with disabilities, our experience and findings provide several implications for practice and future research. Aspects of the intervention to be modified for future efforts are presented.

Vocal Ergonomics in the Workplace: Heating, Ventilation, and Air-Conditioning Method Influences on Vocal Comfort and Function

2017 ◽  
Vol 60 (2) ◽  
pp. 355-363 ◽  
Author(s):  
Mary J. Sandage ◽  
Keith A. Rahn ◽  
Audrey G. Smith
Keyword(s):  
Repeated Measures ◽  
Air Conditioning ◽  
Future Research ◽  
Vocal Function ◽  
Repeated Measures Design ◽  
Work Spaces ◽  
Significant Difference ◽  
Perceived Phonatory Effort ◽  
Forced Air ◽  
And Function

Purpose The purpose of this study was to examine the influence of the heating, ventilation, and air-conditioning method on voice function following a voicing task using ecologically valid offices, one with radiant HVAC and one with forced air. Method A total of 12 consented participants (6 women, 6 men) narrated a video in each of 4 environmental conditions in a within-between repeated-measures design. Acoustic data were collected with an ambulatory phonation monitor and perceived phonatory effort was determined following the voicing task. Data were analyzed using a within-between repeated-measures analysis of variance with significance set at α < .05. Results Perceived phonatory effort did not differ between environments; however, a significant difference in vocal amplitude between 2 trials was identified for the male participants and physiologically significant differences in vocal amplitude were identified for male and female participants. Conclusions The findings suggest that perceived phonatory effort may not be a sensitive measure of vocal function differences following a voicing task in ecologically valid office spaces despite significant acoustic findings. Future research should address longer exposure to environmental differences combined with a longer voicing task within ecologically valid work spaces as well as the recruitment of participants who have particular vulnerability to environmental perturbations.

Using Nature-Inspired Virtual Reality as a Distraction to Reduce Stress and Pain Among Cancer Patients

2020 ◽  
Vol 52 (8) ◽  
pp. 895-918 ◽  
Author(s):  
Diana Scates ◽  
Joan I. Dickinson ◽  
Kathleen Sullivan ◽  
Holly Cline ◽  
Rama Balaraman
Keyword(s):  
Virtual Reality ◽  
Pain Relief ◽  
Repeated Measures ◽  
Treatment Center ◽  
Additional Stress ◽  
Future Research ◽  
Cancer Centers ◽  
Repeated Measures Design ◽  
Port Access ◽  
Chemotherapy Infusion

While many cancer centers suggest treating pain with medication and nondrug treatment, few include the use of virtual reality (VR) as an alternative for stress and pain relief therapy. The purpose of this research was to determine whether a nature-inspired VR simulation reduced stress and pain levels among patients in a cancer treatment center. Using a repeated measures design, 50 patients attending their regularly scheduled chemotherapy infusion were measured for pain and stress during their intravenous (IV)/port access. At the patient’s second visit, they viewed a nature-inspired VR simulation while receiving their IV/port access and were measured for pain and stress again. The paired, one-tailed t tests found significant increases in relaxation, feelings of peace, and positive distractions. While patients felt significantly less frustrated, measures for stress and pain were not significant. Future research should include additional stress and pain measures to determine the viability of VR for chemotherapy infusions.

Sign in / Sign up

Export Citation Format

Share Document