scholarly journals The Effect of Bacillus licheniformis MH48 on Control of Foliar Fungal Diseases and Growth Promotion of Camellia oleifera Seedlings in the Coastal Reclaimed Land of Korea

Pathogens ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 6 ◽  
Author(s):  
Sang-Jae Won ◽  
Jun-Hyeok Kwon ◽  
Dong-Hyun Kim ◽  
Young-Sang Ahn

This study investigated the control of foliar fungal diseases and growth promotion of Camellia oleifera seedlings in coastal reclaimed land through the use of Bacillus licheniformis MH48. B. licheniformis MH48 can produce lytic enzymes chitinase and β-1,3-glucanase that can inhibit foliar pathogens by 37.4 to 50.5%. Nevertheless, foliar diseases appeared in the seedlings with bacterial inoculation, and their survival rate decreased because they were unable to withstand salt stress. However, B. licheniformis MH48 significantly increased the total nitrogen and phosphorus contents in the soils through fixing atmospheric nitrogen and solubilizing phosphorus. The growth of seedlings with bacterial inoculation increased, particularly in root dry weight, by 7.42 g plant−1, which was 1.7-fold greater than that of the control. B. licheniformis MH48 produces the phytohormone auxin, which potentially stimulates seedling root growth. C. oleifera seedlings significantly increased in total nitrogen content to 317.57 mg plant−1 and total phosphorus content to 46.86 mg plant−1. Our results revealed the effectiveness of B. licheniformis MH48 not only in the control of foliar fungal diseases but also in the growth promotion of C. oleifera seedlings in coastal lands.

Forests ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 6 ◽  
Author(s):  
Sang-Jae Won ◽  
Vantha Choub ◽  
Jun-Hyeok Kwon ◽  
Dong-Hyun Kim ◽  
Young-Sang Ahn

This study investigated the control of Fusarium root rot and development of coastal pine (Pinus thunbergii) seedlings in a container nursery by using Bacillus licheniformis MH48. High-quality seedlings without infectious diseases cause vigorous growth. Fusarium root rot caused by Fusarium oxysporum is responsible for serious damage to coastal pine seedlings in nurseries. B. licheniformis MH48 produced enzymes that degraded the fungal cell walls, such as chitinase and β-1,3-glucanase. These lytic enzymes exhibited destructive activity toward F. oxysporum hyphae, which were found to play key roles in the suppression of root rot. In addition, B. licheniformis MH48 increased the nitrogen and phosphorus in soils via fixed atmospheric nitrogen and solubilized inorganic phosphate. B. licheniformis MH48 produced the phytohormone auxin, which stimulated seedling root development, resulting in increased nutrient uptake in seedlings. Both the bacterial inoculation and the chemical fertilizer treatments significantly increased seedling growth and biomass, and the bacterial inoculation had a greater effect on seedling development. Based on the results from this study, B. licheniformis MH48 showed potential as a biological agent against Fusarium root rot and as a promoter of growth and development of Pinus thunbergii seedlings.


2017 ◽  
Vol 41 (2) ◽  
pp. 254-264
Author(s):  
Graciela S. Diniz ◽  
Elisabete Barbarino ◽  
João Oiano-Neto ◽  
Sidney Pacheco ◽  
Sergio O. Lourenço

The amino acid composition and contents of nitrogen, phosphorus, lipid, carbohydrate and protein were determined in muscles of Dactylopterus volitans, Genypterus brasiliensis, Mullus argentinae, Paralichthys patagonicus, Percophis brasiliensis, Pinguipes brasilianus, Rhizoprionodon lalandii, Rhizoprionodon porosus, and Urophycis cirrata. The samples showed low carbohydrate content (<3.5% dry weight in all species) and were rich in protein (>66% dry weight in all species). The percentage of total lipid varied widely among species, and M. argentinae showed the highest concentrations (16%). The percentage of nitrogen and phosphorus was high and similar among species, with overall average values of 13.3% and 1.2%, respectively. The amino acids composition was similar among the animals, with glutamic acid and lisine as the most abundant amino acid and histidine in low concentrations. Among species, the content of proteinaceous nitrogen was high, with an average of 96.8% of the total nitrogen. From data of total amino acid and total nitrogen, specific nitrogen-to-protein conversion factors were calculated for each species. The nitrogen-toprotein conversion factors ranged from 5.39 to 5.98, with an overall average of 5.71. These findings showed that the traditional conversion factor of 6.25 overestimates the actual protein content and should be avoided.


2020 ◽  
Vol 13 (2) ◽  
pp. 54-65 ◽  
Author(s):  
M.E.A. Bendaha ◽  
H.A. Belaouni

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 468b-468
Author(s):  
Stephen F. Klauer ◽  
J. Scott Cameron ◽  
Chuhe Chen

After promising results were obtained with an open-style split trellis (two top wires) in its initial year, two new trials were established in 1997 in northwest (Lynden) and southwest (Woodland) Washington. For the split trellis, actual yields were 33% (machine-picked 1/2 season) and 17% (hand-picked) greater, respectively, for the two locations compared to the conventional trellis (one top wire). In Woodland, canes from the split trellis had 33% more berries, 55% more laterals, 69% more leaves, and 25% greater leaf area compared with the conventional trellis. Greatest enhancement of these components was in the upper third of the canopy. Laterals were also shorter in this area of the split canopy, but there was no difference in average total length of lateral/cane between trellis types. Total dry weight/cane was 22% greater in the split trellis, but component partitioning/cane was consistent between the two systems with fruit + laterals (43%) having the greatest above-ground biomass, followed by the stem (30% to 33%) and the leaves (21% to 22%). Measurement of canopy width, circumference, and light interception showed that the split-trellis canopy filled in more quickly, and was larger from preanthesis through postharvest. Light interception near the top of the split canopy was 30% greater 1 month before harvest with 98% interception near the top and middle of that canopy. There was no difference between the trellis types in leaf CO2 assimilation, spectra, or fluorescence through the fruiting season, or in total nitrogen of postharvest primocane leaves.


1996 ◽  
Vol 31 (3) ◽  
pp. 485-504 ◽  
Author(s):  
Patricia Chow-Fraser ◽  
Barb Crosbie ◽  
Douglas Bryant ◽  
Brian McCarry

Abstract During the summer of 1994, we compared the physical and nutrient characteristics of the three main tributaries of Cootes Paradise: Spencer, Chedoke and Borer’s creeks. On all sampling occasions, concentrations of CHL α and nutrients were always lowest in Borer’s Creek and highest in Chedoke Creek. There were generally 10-fold higher CHL α concentrations and 2 to 10 times higher levels of nitrogen and phosphorus in Chedoke Creek compared with Spencer Creek. Despite this, the light environment did not differ significantly between Spencer and Chedoke creeks because the low algal biomass in Spencer Creek was balanced by a relatively high loading of inorganic sediments from the watershed. Laboratory experiments indicated that sediments from Chedoke Creek released up to 10 µg/g of soluble phosphorus per gram (dry weight) of sediment, compared with only 2 µg/g from Spencer Creek. By contrast, sediment samples from Spencer Creek contained levels of polycyclic aromatic hydrocarbon that were as high as or higher than those from Chedoke Creek, and much higher than those found in Borer’s Creek. The distribution of normalized PAH concentrations suggests a common source of PAHs in all three tributaries, most likely automobile exhaust, since there were high concentrations of fluoranthene and pyrene, both of which are derivatives of engine combustion.


2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Geetha Rajendran ◽  
Maheshwari H. Patel ◽  
Sanket J. Joshi

One of the ways to increase the competitive survivability of rhizobial biofertilizers and thus achieve better plant growth under such conditions is by modifying the rhizospheric environment or community by addition of nonrhizobial nodule-associated bacteria (NAB) that cause better nodulation and plant growth when coinoculated with rhizobia. A study was performed to investigate the most commonly associated nodule-associated bacteria and the rhizospheric microorganisms associated with theFenugreek(Trigonella foenum-graecum) plant. Isolation of nonrhizobial isolates from root nodules ofFenugreekwas carried out along with the rhizospheric isolates. About 64.7% isolates obtained fromFenugreeknodules were gram-negative coccobacilli, 29.41% were gram-positive bacilli, and all rhizospheric isolates except one were gram-positive bacilli. All the isolates were characterized for their plant growth promoting (PGP) activities. Two of the NAB isolates M2N2c and B1N2b (Exiguobacterium sp.) showed maximum positive PGP features. Those NAB isolates when coinoculated with rhizobial strain—S. meliloti, showed plant growth promotion with respect to increase in plant’s root and shoot length, chlorophyll content, nodulation efficiency, and nodule dry weight.


1977 ◽  
Vol 4 (5) ◽  
pp. 799 ◽  
Author(s):  
I Sofield ◽  
IF Wardlaw ◽  
LT Evans ◽  
SY Zee

Plants of five cultivars of wheat were grown under controlled-environmental conditions in order to analyse the effect of cultivar and of temperature and illuminance after anthesis on the accumulation of nitrogen and phosphorus by grains in relation to dry matter. The water relations of the grain during maturation were also examined, using calcium content as an index of water entry. The nitrogen and phosphorus contents of grains increased linearly throughout the grain growth period. The percentage of nitrogen and phosphorus in grains fell sharply during the first few days after anthesis but rose progressively thereafter. The higher the temperature, and the lower the illuminance, the higher was the percentage of nitrogen in the grain of all cultivars. Such conditions also reduce final grain size, but their effects on nitrogen concentration in the grain were apparent early in grain development. No evidence was found of a flush of nitrogen or phosphorus into the grain late in its development. Water entry into the grain continued at a steady rate until maximum grain dry weight was reached, then ceased suddenly. No evidence was found of an increased rate of water loss by the grain at that stage, and the rapid fall in water content at the cessation of grain growth may have been due to blockage of the chalazal zone of entry into the grain by the deposition of lipids. Accumulation of dry matter, nitrogen and phosphorus and entry of water into the grain all ceased at the time of lipid deposition in the chalazal zone.


1995 ◽  
Vol 11 (3) ◽  
pp. 333-350 ◽  
Author(s):  
Nicholas C. Songwe ◽  
D. U. U. Okali ◽  
F. E. Fasehun

ABSTRACTDecomposition of litter on the forest floor and of leaves of five species, Celtis zenkeri, Cola lepidota, Desbordesia glaucescens, Ceiba pentandra and Terminalia superba in nylon mesh bags, as well as wood decay were studied in the tropical rainforest at Southern Bakundu Forest Reserve, Cameroon.The rate of loss of dry matter was fastest in Celtis zenkeri which was significantly different from the other species, while potassium was the most rapidly released element from all species with more than 50% being released in the first two months of the experiment. Nitrogen and phosphorus showed initial increases in bagged leaf litter independent of dry weight losses and while nitrogen was later released phosphorus continued to increase reaching 2–3 times the initial concentration. Decomposition constant (k) of litter on the forest floor was found to be 2.23 whereas the mean decomposition constants of the different species were as follows: Celtis zenkeri 4.18, Cola lepidota 2.18, Desbordesia glaucescens 1.60 and Ceiba pentandra 2.16 for the two experiments.Termites were found to have a very great influence on the decay of the wood of Terminalia superba with decay due to micro-organisms being negligible.


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