Light-Activated Liposomes Coated with Hyaluronic Acid as a Potential Drug Delivery System

Light-activated liposomes permit site and time-specific drug delivery to ocular and systemic targets. We combined a light activation technology based on indocyanine green with a hyaluronic acid (HA) coating by synthesizing HA–lipid conjugates. HA is an endogenous vitreal polysaccharide and a potential targeting moiety to cluster of differentiation 44 (CD44)-expressing cells. Light-activated drug release from 100 nm HA-coated liposomes was functional in buffer, plasma, and vitreous samples. The HA-coating improved stability in plasma compared to polyethylene glycol (PEG)-coated liposomes. Liposomal protein coronas on HA- and PEG-coated liposomes after dynamic exposure to undiluted human plasma and porcine vitreous samples were hydrophilic and negatively charged, thicker in plasma (~5 nm hard, ~10 nm soft coronas) than in vitreous (~2 nm hard, ~3 nm soft coronas) samples. Their compositions were dependent on liposome formulation and surface charge in plasma but not in vitreous samples. Compared to the PEG coating, the HA-coated liposomes bound more proteins in vitreous samples and enriched proteins related to collagen interactions, possibly explaining their slightly reduced vitreal mobility. The properties of the most abundant proteins did not correlate with liposome size or charge, but included proteins with surfactant and immune system functions in plasma and vitreous samples. The HA-coated light-activated liposomes are a functional and promising alternative for intravenous and ocular drug delivery.

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Effects of the Molecular Weight of Hyaluronic Acid in a Carbon Nanotube Drug Delivery Conjugate

Frontiers in Chemistry ◽

10.3389/fchem.2020.578008 ◽

2020 ◽

Vol 8 ◽

Author(s):

Silvia Arpicco ◽

Michał Bartkowski ◽

Alessandro Barge ◽

Daniele Zonari ◽

Loredana Serpe ◽

...

Keyword(s):

Drug Delivery ◽

Molecular Weight ◽

Hyaluronic Acid ◽

Anticancer Agents ◽

Targeted Delivery ◽

Molecular Weights ◽

Good Biocompatibility ◽

Highly Hydrophobic ◽

Cluster Of Differentiation ◽

Walled Carbon Nanotubes

Hyaluronic acid (HA) is a ubiquitous biopolymer involved in many pathophysiological roles. One HA receptor, the cluster of differentiation CD44 protein, is often overexpressed in tumor cells. As such, HA has attracted considerable interest in the development of drug delivery formulations, given its intrinsic targetability toward CD44 overexpressing cells. The present study is focused on examining the correlation of HA molecular weight with its targetability properties. A library of conjugates obtained by linking the amino group of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE) to the carboxylic residues of HA of different molecular weight (6.4, 17, 51, 200, and 1,500 kDa) were synthesized and fully characterized. The HA-DMPE conjugates were then used to non-covalently functionalize the highly hydrophobic single-walled carbon nanotubes (CNT), and further encapsulate the anticancer drug doxorubicin (DOX). Our results show that the complexes DOX/CNT/HA-DMPE maintain very good and stable dispersibility. Drug release studies indicated a pH-responsive release of the drug from the nanocarrier. Cell viability tests demonstrated that all HA modified CNTs have good biocompatibility, and specific targeting toward cells overexpressing the CD44 receptor. Among all the molecular weights tested, the 200 kDa HA showed the highest increase in cellular uptake and cytotoxic activity. All these promising attributes make CNT/HA200-DMPE a “smart” platform for tumor-targeted delivery of anticancer agents.

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Photo-crosslinked hyaluronic acid nanoparticles with improved stability for in vivo tumor-targeted drug delivery

Biomaterials ◽

10.1016/j.biomaterials.2013.03.050 ◽

2013 ◽

Vol 34 (21) ◽

pp. 5273-5280 ◽

Cited By ~ 65

Author(s):

Hong Yeol Yoon ◽

Heebeom Koo ◽

Ki Young Choi ◽

Ick Chan Kwon ◽

Kuiwon Choi ◽

...

Keyword(s):

Drug Delivery ◽

Hyaluronic Acid ◽

Targeted Drug Delivery ◽

Improved Stability ◽

Targeted Drug

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A Review on Microsponge Drug Delivery System

International Journal of Review in Life Sciences ◽

10.26452/ijrls.v10i2.1280 ◽

2020 ◽

Vol 10 (2) ◽

pp. 53-59

Author(s):

Bharathi M ◽

Mullaikodi O ◽

Rajalingam D ◽

Gnanasekar N ◽

Kesavan M

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Delivery System ◽

Physical Stability ◽

Current Investigation ◽

Considerable Time ◽

Polymeric Structure ◽

Point Of Interest ◽

Time Period ◽

Time Specific

A Microsponge (MS) is an extremely interconnected, permeable, polymeric structure that involves permeable microparticles trapping and discharging through the skin for a considerable time period. Drug delivery system (DDS) offer extended discharge with less degradation, improved physical stability along with better tolerance. The main intend of any DDS is to achieve the required amount of drug in plasma to produce the desired therapeutic and non-poisonous effect over a prolonged period of time. Specific methods for preparing MS were reviewed in this current investigation, and their pharmaceutical implementations were signed. MS have major DDS point of interest. It also improves stability, increased flexibility in formulation and increased elegance. In fact, numerous studies have reported that MS supplies are not allergic, mutagenic, and poisonous. MS creativity is used in products such as sunscreen, prescription, cosmetics, and OTC skin care. This inquiry primarily focuses on the different methods used to identify, plan and exploit MS.

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Monocyte as an Emerging Tool for Targeted Drug Delivery: A Review

Current Pharmaceutical Design ◽

10.2174/1381612825666190102104642 ◽

2019 ◽

Vol 24 (44) ◽

pp. 5296-5312 ◽

Cited By ~ 5

Author(s):

Fakhara Sabir ◽

Rai K. Farooq ◽

Asim.ur.Rehman ◽

Naveed Ahmed

Keyword(s):

Drug Delivery ◽

Targeted Drug Delivery ◽

The Body ◽

Carrier System ◽

Bone Marrow Precursor ◽

Extensive Introduction ◽

Cancer Inflammation ◽

Targeted Drug ◽

Cluster Of Differentiation

Monocytes are leading component of the mononuclear phagocytic system that play a key role in phagocytosis and removal of several kinds of microbes from the body. Monocytes are bone marrow precursor cells that stay in the blood for a few days and migrate towards tissues where they differentiate into macrophages. Monocytes can be used as a carrier for delivery of active agents into tissues, where other carriers have no significant access. Targeting monocytes is possible both through passive and active targeting, the former one is simply achieved by enhanced permeation and retention effect while the later one by attachment of ligands on the surface of the lipid-based particulate system. Monocytes have many receptors e.g., mannose, scavenger, integrins, cluster of differentiation 14 (CD14) and cluster of differentiation 36 (CD36). The ligands used against these receptors are peptides, lectins, antibodies, glycolipids, and glycoproteins. This review encloses extensive introduction of monocytes as a suitable carrier system for drug delivery, the design of lipid-based carrier system, possible ways for delivery of therapeutics to monocytes, and the role of monocytes in the treatment of life compromising diseases such as cancer, inflammation, stroke, etc.

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Recent Advances in the Development of Polymeric Nanocarrier Formulations for the Treatment of Colon Cancer

Drug Delivery Letters ◽

10.2174/2210303108666181109120710 ◽

2019 ◽

Vol 9 (1) ◽

pp. 2-14

Author(s):

Sahil Kumar ◽

Bandna Sharma ◽

Kiran Thakur ◽

Tilak R. Bhardwaj ◽

Deo N. Prasad ◽

...

Keyword(s):

Drug Delivery ◽

Colon Cancer ◽

Hyaluronic Acid ◽

Targeted Drug Delivery ◽

Drug Delivery Systems ◽

Delivery Systems ◽

Near Ir ◽

Targeted Drug ◽

Poly Ethylene ◽

Novel Drug

Background: Many efforts have been explored in the last decade to treat colon cancer but nanoparticulate drug delivery systems are making a vital contribution in the improvement of drug delivery to colon cancer cells. Objective: In this review, we attempt to highlight recent advancements in the development of novel drug delivery systems of nanoparticles for the targeted drug delivery to colon. Polymers like Epithelial Cell Adhesion Molecule (EpCAM) aptamer chitosan, Hyaluronic Acid (HA), Chitosan (CS)– Carboxymethyl Starch (CMS), silsesquioxane capped mesoporous silica, Near IR (NIR) fluorescent Human Serum Albumin (HAS), poly(ethylene glycol)-conjugated hyaluronic acid etc. have been discussed by employing various anticancer drugs like doxorubicin, oxaliplatin, paclitaxel, 5-fluorouracil etc. Conclusion: These novel drug delivery systems have been determined to be more efficacious in terms of stability, sustained and targeted drug delivery, therapeutic efficacy, improved bioavailability and enhanced anticancer activity.

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Design and Optimization of Chronotherapeutic Dosage Form for the Treatment of Angina Pectoris

Drug Delivery Letters ◽

10.2174/2210303110999200910103909 ◽

2020 ◽

Vol 10 ◽

Author(s):

Rupali Singh ◽

Rishabha Malviya

Keyword(s):

Drug Delivery ◽

Angina Pectoris ◽

Ethyl Cellulose ◽

Dosage Form ◽

Cellulose Solution ◽

Specific Drug ◽

Pharmaceutical Dosage Form ◽

Weight Variation ◽

Short Period ◽

Time Specific

Background: The chronotherapy concept attains considerable focus towards itself due to its pulsatile fashion rather than continuous delivery. The delivery of the right amount of drug to the target organ at the most appropriate time is fulfilled by using the chronotherapeutic dosage form. Aim: The present study aims to develop and evaluate a chronotherapeutic drug delivery system by using natural polymer for time specific drug delivery at the target site. Material and Method: Tamarind seed polysaccharide was extracted and used in the preparation of core tablets. Nine formulations of core tablets were prepared with nifedipine at 5 tonnes of pressure on 6 mm punch. The core tablets were prepared by using the compression coating method. The three batches F1, F2 and F3 were prepared by using tamarind gum in different concentration i.e. 45%, 22.5% and 67.5% respectively and compressed at 8 tonnes of pressure on 12 mm of punch. The finally compressed tablet was coated with different concentrations of ethyl cellulose in which isopropyl alcohol used as a solvent. In a controlled medium, a stability study was performed to evaluate the physical appearance, drug content and release of the prepared core tablet. Result: All the nine formulations of tablets were prepared successfully and the evaluation studies (thickness, weight variation, hardness, friability etc.) revealed that all the formulations were within the official range. The release study of the drug revealed that the formulation F7 containing 67.5% of tamarind polymer, coated with 2%, 4% and 5% of ethyl cellulose solution released 59.68±1.03% (Q50%) drug within 5 h whereas, 87.09±2.08% (Q80%) within 6 h and within 12 h 97.74±2.19% of the drug was released. The formulation F7 was found to be more effective as it released the maximum amount of drug in a short period as compared with other formulations. Conclusion: The coating of core tablets allowed to prepare pharmaceutical dosage form for time specific drug delivery. These chronotherapeutic core tablets can be used for the treatment of angina pectoris and hypertension etc.

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