scholarly journals Impact of Foliar Application of Chitosan Dissolved in Different Organic Acids on Isozymes, Protein Patterns and Physio-Biochemical Characteristics of Tomato Grown under Salinity Stress

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 388 ◽  
Author(s):  
Mohamed S. Attia ◽  
Mahmoud S. Osman ◽  
Amr S. Mohamed ◽  
Hany A. Mahgoub ◽  
Mohamed O. Garada ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Enzymes ◽  
Citric Acid ◽  
Organic Acids ◽  
Salinity Stress ◽  
Photosynthetic Pigments ◽  
Morphological Traits ◽  
Foliar Application ◽  
Protein Patterns ◽  
Tomato Plants

In this study, the anti-stress capabilities of the foliar application of chitosan, dissolved in four different organic acids (acetic acid, ascorbic acid, citric acid and malic acid) have been investigated on tomato (Solanum lycopersicum L.) plants under salinity stress (100 mM NaCl). Morphological traits, photosynthetic pigments, osmolytes, secondary metabolites, oxidative stress, minerals, antioxidant enzymes activity, isozymes and protein patterns were tested for potential tolerance of tomato plants growing under salinity stress. Salinity stress was caused a reduction in growth parameters, photosynthetic pigments, soluble sugars, soluble proteins and potassium (K+) content. However, the contents of proline, ascorbic acid, total phenol, malondialdehyde (MDA), hydrogen peroxide (H2O2), sodium (Na+) and antioxidant enzyme activity were increased in tomato plants grown under saline conditions. Chitosan treatments in any of the non-stressed plants showed improvements in morphological traits, photosynthetic pigments, osmolytes, total phenol and antioxidant enzymes activity. Besides, the harmful impacts of salinity on tomato plants have also been reduced by lowering MDA, H2O2 and Na+ levels. Chitosan treatments in either non-stressed or stressed plants showed different responses in number and density of peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD) isozymes. NaCl stress led to the diminishing of protein bands with different molecular weights, while they were produced again in response to chitosan foliar application. These responses were varied according to the type of solvent acid. It could be suggested that foliar application of chitosan, especially that dissolved in ascorbic or citric acid, could be commercially used for the stimulation of tomato plants grown under salinity stress.

scholarly journals Promotion of Growth and Physiological Characteristics in Water-Stressed Triticum aestivum in Relation to Foliar-Application of Salicylic Acid

Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1316
Author(s):  
Abida Parveen ◽  
Muhammad Arslan Ashraf ◽  
Iqbal Hussain ◽  
Shagufta Perveen ◽  
Rizwan Rasheed ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Salicylic Acid ◽  
Antioxidant Enzymes ◽  
Water Stress ◽  
Photosynthetic Pigments ◽  
Foliar Spray ◽  
Field Capacity ◽  
Available Water ◽  
Drought Tolerant ◽  
Growth Attributes

The present work reports the assessment of the effectiveness of a foliar-spray of salicylic acid (SA) on growth attributes, biochemical characteristics, antioxidant activities and osmolytes accumulation in wheat grown under control (100% field capacity) and water stressed (60% field capacity) conditions. The total available water (TAW), calculated for a rooting depth of 1.65 m was 8.45 inches and readily available water (RAW), considering a depletion factor of 0.55, was 4.65 inches. The water contents corresponding to 100 and 60% field capacity were 5.70 and 1.66 inches, respectively. For this purpose, seeds of two wheat cultivars (Fsd-2008 and S-24) were grown in pots subjected to water stress. Water stress at 60% field capacity markedly reduced the growth attributes, photosynthetic pigments, total soluble proteins (TSP) and total phenolic contents (TPC) compared with control. However, cv. Fsd-2008 was recorded as strongly drought-tolerant and performed better compared to cv. S-24, which was moderately drought tolerant. However, water stress enhanced the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and membrane electrolyte leakage (EL) and modulated the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as accumulation of ascorbic acid (AsA), proline (Pro) and glycine betaine (GB) contents. Foliar-spray with salicylic acid (SA; 0, 3 mM and 6 mM) effectively mitigated the adverse effects of water stress on both cultivars. SA application at 6 mM enhanced the shoot and root length, as well as their fresh and dry weights, and improved photosynthetic pigments. SA foliage application further enhanced the activities of antioxidant enzymes (SOD, POD, and CAT) and nonenzymatic antioxidants such as ascorbic acid and phenolics contents. However, foliar-spray of SA reduced MDA, H2O2 and membrane permeability in both cultivars under stress conditions. The results of the present study suggest that foliar-spray of salicylic acid was effective in increasing the tolerance of wheat plants under drought stress in terms of growth attributes, antioxidant defense mechanisms, accumulation of osmolytes, and by reducing membrane lipid peroxidation.

scholarly journals Study of Seed Soaking and Foliar Application of Ascorbic Acid, Citric Acid and Humic Acid on Growth, Yield and Active Components In Maize

2021 ◽  
Vol 910 (1) ◽  
pp. 012076
Author(s):  
J. J. Kadhim ◽  
J. H. Hamza
Keyword(s):  
Ascorbic Acid ◽  
Citric Acid ◽  
Humic Acid ◽  
Foliar Application ◽  
Block Design ◽  
Growth Yield ◽  
Active Components ◽  
Seed Soaking ◽  
Maize Seeds ◽  
Biological Yield

Abstract Foliar application and seed soaking has been used as a means of supplying supplemental doses of nutrients, plant hormones, stimulants, and organic components. the effects of these applications have included yield increases, and improved drought tolerance, and enhanced crop quality, so A field experiment was carried out during spring seasons in 2019 and 2020 for styding Seed soaking and Foliar Application of Ascorbic acid, Citric acid and Humic acid on Growth, Yield and Active Components IN Maize. Randomized complete block design in split plots arrangement was used with three replicates. Main-plots were for seeds soaking with ascorbic, citric (100 mg l-1) frequently and humic at (1 ml l-1). Sub-plots were for vegetative parts nutrition with same acids above. Results showed a significant superiority of seeds soaking in humic acid for traits of ears number per plant (1.3 and 1.6), rows number per ear (16.6 and 17.5), grains number per row (39.3 and 45.3), grains number per ear (644.3 and 793.5), weight of 300 grains (75.3 and 100.6 g), total grain yield ( 6.0 and 8.3 ton ha-1), shelling ratio (79.3 and 85.1%), biological yield (20.7 and 26.8 ton ha-1), harvesting index (30.4 and 31.2%), in both seasons respectively. Effect of vegetative parts nutrition or interaction between studied factors was non-significant on most traits studied. It can be concluded that soaking maize seeds in humic acid improves yield and yield components.

scholarly journals INHIBITION OF BACTERIA ISOLATED FROM NINH THUAN GRAPES BY ORGANIC ACIDS

2018 ◽  
Vol 54 (4A) ◽  
pp. 290
Author(s):  
Nguyen Thi Hanh
Keyword(s):  
Ascorbic Acid ◽  
Lactic Acid ◽  
Citric Acid ◽  
Bacillus Thuringiensis ◽  
Organic Acids ◽  
Bacillus Cereus ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Dilution Method ◽  
Minimal Bactericidal Concentration

Ninh Thuan grapes are famous specialty of Vietnam. After harvesting, they are very susceptible to damage if not preserved in time. This study aimed to find the minimal inhibitory concentration and minimal bactericidal concentrations of organic acids, such as lactic acid, citric acid, and ascorbic acid towards seven bacteria previously isolated from Ninh thuan grapes (Empedobacter  brevis, Citrobacter sp. Enterobacterium ludwigii, Bacillus cereus, Flavobacterium sp., Pseudomonas oryzihabitans and Bacillus thuringiensis) in order to apply in pretreatment of grapes prior to storage. The Minimal Inhibitory Concentration (MIC) was determined by the binary dilution method. 0.1 ml of each dilution of test acids with initial concentration of 3 % was mixed with 0.1 ml of bacteria (c.a. 5.105 CFU/ml), in 96-culturing-well plate. Innoculated plate was incubated for 24 hours at temperature of 37 °C. Optical density was measured at 620 nm wavelength by a Microplate reader device. For Minimal Bactericidal Concentration (MBC) determination, 0.1 ml of bacteria (c.a.5.105 CFU/ml) was mixed with 0.4 ml liquid medium of Tryptone Glucose Agar (TGA) and of 0.5 ml acid at above concentrations, culturing within 24 hours at temperature of 37 °C. After 24 hours, they were dropped onto TGA agar plates, and cultured for 24 hours at 37 °C. The results showed that, the MIC and MBC of citric acid toward Empedobacter brevis were 0.12 mg/ml and 0.12 mg/ml; Citrobacter sp. were 0.9 mg/ml and 3.8 mg/ml; Enterobacterium ludwigii were 0.45 mg/ml and 1.9 mg/ml; Bacillus cereus were  0.9 mg/ml and 0.9 mg/ml; Flavobacterium sp. were 0.12 mg/ml  and 0.45 mg/ml; Pseudomonas oryzihabitans were 0.12 mg/ml and 0.45 mg/ml and Bacillus thuringiensis were 0.12 mg/ml  and 0.9 mg/ml, respectively. Similarly, MIC and MBC of  lactic acid toward: Empedobacter brevis were 0.12 mg/ml and 0.23 mg/ml;Citrobacter sp. were 0.9 mg/ml and 1.9 mg/ml; Enterobacterium ludwigii were 0.45 mg/ml and 0.9 mg/ml; Bacillus cereus were 0.23 mg/ml and 0.45 mg/ml; Flavobacterium sp. were 0.12 mg/ml and 1.9 mg/ml;  Pseudomonas oryzihabitans  were 0.23 mg/ml and 1.9 mg/ml and Bacillus thuringiensis were 0.12 mg/ml and 1.9 mg/ml, respectively. Ascorbic acid was found to be inefficient for use as antimicrobial agent against isolated bacteria. The above results suggested that citric and lactic acids could be used at maximal concentrations of 3.8 mg/ml and 1.9 mg/ml, respectively to suppress bacteria from grapes. This finding would contribute to develop method for pretreatment of grapes in fresh grape preservation techniques. 

scholarly journals Some Pomological Properties of Promising Seed Propagated Walnut Genotypes from Inner Turkey

2019 ◽  
Vol 47 (4) ◽  
pp. 1094-1099
Author(s):  
Aysen KOC ◽  
Hakan KELES ◽  
Sezai ERCISLI
Keyword(s):  
Fatty Acids ◽  
Ascorbic Acid ◽  
Linoleic Acid ◽  
Citric Acid ◽  
Organic Acids ◽  
Fumaric Acid ◽  
Juglans Regia ◽  
Kernel Weight ◽  
Digital Object Identifier ◽  
Digital Object

In this study, fruits from seed propagated walnut (Juglans regia L.) trees were collected two consecutive years in harvest seasons in Yozgat province in Turkey.  Considering two years results, promising five genotypes were determined as cultivar candidate. In the promising genotypes, nut weight ranged from 12.55 (Y11) to 15.08 g (Y15), kernel weight ranged from 5.23 (Y11) to 7.34 g (Y15) and kernel ratio varied between 41.67 (Y11) to 50.84% (Y1), respectively. Linoleic acid was the only polyunsaturated fatty acids and oleic, palmitoleic and gondoic acids determined as major monounsaturated acids ranged from 30.36 to 48.43%, 0.05 to 0.14% and 0.22 to 0.29%, respectively. Propylparaben was the major phenolic acid among the determined phenolic acids in fruits of all five promising genotypes and Y16 had the highest amount of propylparaben (128.08 mg per kg) in its kernel. Malic and tartaric acid were the major organic acids in walnut kernels ranged from 47.88 to 78.51 mg per 100 g and 30.27 to 49.60 mg per 100 g, respectively. L-ascorbic acid was the another organic acids in walnut kernels ranged from 10.71 to 19.71 mg per 100 g. Citric acid was non-determined in kernels of Y1, Y14 and Y15 but determined at kernels of Y11 and Y16 as 4.51 and 7.55 mg per 100 g, respectively. It was determined that the oxalic, malonic, succinic, maleic and fumaric acid contents varied between 8.39-12.08 mg per 100 g, 6.02-9.19 mg per 100 g, 2.86-5.32 mg per 100 g, 0.26-3.00 mg per 100 g and 0.26-0.58 mg per 100 g, respectively.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

scholarly journals Effect of Bagging on the Content of Sugar and Acid in Postharvest "Jinhuang" Mango Fruit

2020 ◽  
Vol 145 ◽  
pp. 01032
Author(s):  
Changbin Wei ◽  
Jian Qiao ◽  
Xinming Tang ◽  
Qingze Yan ◽  
Lizhu Tang ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Citric Acid ◽  
Organic Acids ◽  
Fruit Quality ◽  
Hplc Method ◽  
Postharvest Storage ◽  
Mango Fruit ◽  
Acid Ratio

The glucose, fructose, sucrose, and organic acids in the pulp of “Jinhuang” mango were analyzed using the HPLC method and the effect of bagging on fruit quality was researched during the postharvest storage. The results showed that there was a certain difference in the content of sugar and acid among the three treatments. The effect of bagging treatments on fructose, glucose, and sucrose in the fruit of “Jinhuang” mango was mainly reflected in sucrose. The treatments of white bagging (WB) and black bagging (BB) delayed the production of sucrose and the decomposition of citric acid, and increased the content of ascorbic acid and quinine. Based on sugar-acid ratio, the flavor of the three treatments was evaluated as: CK> WB> BB. The fruit quality of “Jinhuang” mango was affected by bagging treatments to a certain extent.

scholarly journals Analysis of the influence and optimization of concentration of organic acids on chemical and physical properties of wheat dough using a response surface methodology and desirability function

2013 ◽  
Vol 67 (1) ◽  
pp. 103-113 ◽  
Author(s):  
Olivera Simurina ◽  
Bojana Filipcev ◽  
Pavle Jovanov ◽  
Bojana Ikonic ◽  
Dragana Simovic-Soronja
Keyword(s):  
Experimental Data ◽  
Ascorbic Acid ◽  
Response Surface Methodology ◽  
Citric Acid ◽  
Organic Acids ◽  
Physical Properties ◽  
Regression Coefficient ◽  
Desirability Function ◽  
Dough Extensibility ◽  
Main Effects

In order to improve physical and chemical properties of dough produced from wheat flour of suboptimal quality (protein content 10.7% dry basis, dough energy 4.0 cm2), optimal doses of ascorbic and citric acid were evaluated using a response surface methodology and desirability function. The paper brings the analysis of the main effects as well as their interactions. The effect of organic acids was evident in relation to pH lowering and decrease in free thiol groups, which consequently changed the physical properties of dough (increased dough energy, extensibility and resistance). The well known oxidative effect of ascorbic acid which is manifested as increase in dough energy and resistance, was enhanced by the addition of citric acid i.e. their synergistic action. Contribution of citric acid was the donation of hydrogen ions which changed the pH, lowered the content of free -SH groups and increased protein aggregation. Ascorbic acid individually significantly increased energy (linear regression coefficient b1 = 4.010-4) but higher effect was exerted by the addition of ascorbic and citric acid mixture as seen through higher interaction regression coefficient (b12 = 0.076). Dough resistance was significantly affected only by ascorbic acid due to its oxidizing action whereas dough extensibility was affected by both acids (main effects) at all applied doses and their mixtures. The effect on dough extensibility depends on the dose of acids but resistance decreased with quadratic increase of acid doses. Second-order polynomials were used in modeling of responses (dough energy, resistance and extensibility) which showed a good fit with experimental data as shown by high values of the coefficients of determination R2 for energy, resistance and extensibility (0.953, 0.976 and 0.996, respectively). Based on F value, it could be concluded that the model gave good prediction of experimental data while p-values for all responses showed that the models were significant at significance of 90%. The obtained models were used to optimize the doses of ascorbic and citric acid in order to maximize dough energy and extensibility. Significant increase of dough energy by 4.7 times and extensibility by 1.5 times was achieved by the set of optimum conditions of 97 mg/kg citric and 100 mg/kg ascorbic acid. The obtained results are applicable in semi-industrial and industrial facilities for flour processing.

scholarly journals 2-Keto-L-Gulonic Acid Improved the Salt Stress Resistance of Non-heading Chinese Cabbage by Increasing L-Ascorbic Acid Accumulation

2021 ◽  
Vol 12 ◽  
Author(s):  
Mingfu Gao ◽  
Hao Sun ◽  
Meijun Shi ◽  
Qiqi Wu ◽  
Dongxu Ji ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Photosynthetic Pigments ◽  
Chinese Cabbage ◽  
Stress Resistance ◽  
Effective Means ◽  
Membrane Damage ◽  
Future Application ◽  
Heading Chinese Cabbage

Salt stress has long been a prominent obstacle that restricts crop growth, and increasing the L-ascorbic acid (ASA) content of crops is an effective means of alleviating this stress. 2-Keto-L-gulonic acid (2KGA) is a precursor used in industrial ASA production as well as an ASA degradation product in plants. However, to date, no study has investigated the effects of 2KGA on ASA metabolism and salt stress. Here, we evaluated the potential of using 2KGA to improve crop resistance to salt stress (100mM NaCl) through a cultivation experiment of non-heading Chinese cabbage (Brassica campestris ssp. chinensis). The results showed that the leaf and root biomass were significantly improved by 2KGA application. The levels of metabolites and enzymes related to stress resistance were increased, whereas the hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents were decreased. Lipid peroxidation and cell membrane damage were alleviated following 2KGA treatment. Positive correlations were found between photosynthetic pigments and organic solutes, ASA and photosynthetic pigments, and ASA and antioxidant enzymes. In contrast, negative correlations were observed between antioxidant enzymes and H2O2/MDA. Moreover, the expression levels of L-gulono-1,4-lactone oxidase, GDP-mannose pyrophosphorylase, dehydroascorbate reductase-3, and ascorbate peroxidase were increased by 2KGA treatment. These results suggested that exogenous 2KGA application can relieve the inhibitory effect of salt stress on plant growth, and the promotion of ASA synthesis may represent a critical underlying mechanism. Our findings have significant implications for the future application of 2KGA or its fermentation residue in agriculture.

scholarly journals Efecto d Baño Químico Con Ácido Cítrico y Ascórbico, Temperatura y Tiempo de Almacenamiento Sobre el Pardeamiento Enzimático en Persea Americana Mínimamente Procesada

2021 ◽  
Vol 17 (37) ◽  
pp. 247
Author(s):  
Lurys Ivette Martínez Marín ◽  
Angie Macías
Keyword(s):  
Ascorbic Acid ◽  
Food Safety ◽  
Citric Acid ◽  
Organic Acids ◽  
Significant Loss ◽  
Persea Americana ◽  
Enzymatic Browning ◽  
Minimally Processed ◽  
Different Temperatures ◽  
Loss Of Mass

Se evaluó el efecto de los ácidos orgánicos sobre el pardeamiento enzimático de la Persea americana variedad Hass, mínimamente procesada, empacada al vacío y almacenada a distintas temperaturas. Las muestras de aguacate se trataron con un baño químico de ácidos orgánicos (ácido cítrico y ácido ascórbico) con tres concentraciones diferentes, luego se empacaron al vacío y fueron almacenadas a 4° y 8° C durante 12 días. Muestras sin tratamiento se emplearon como control y todas las muestras se evaluaron cada 0, 4, 8 y 12 días. Se concluye que a 8°C el color y el sabor del aguacate mejora. Durante cada observación no hubo pérdida significativa de masa. Estos resultados, aunque incipientes, son útiles porque pueden contribuir con la seguridad alimentaria, además de determinar nuevas formas de preservar Persea americana y poder comercializarla como un producto de cuarta gama. The effect of organic acids on the enzymatic browning of Persea americana variety Hass, minimally processed, vacuum packed and stored at different temperatures, was evaluated. The avocado samples were treated with a chemical bath of organic acids (citric acid and ascorbic acid) with three different concentrations, then they were vacuum packed and stored at 4 ° and 8 ° C for 12 days. Untreated samples were used as control and all samples were evaluated at 0, 4, 8 and 12 days. It is concluded that at 8 ° C the color and flavor of the avocado improves; the chemical bath that obtained the best results was chemical bath 3 with respect to color and the samples are best preserved on day 8. During each observation there was no significant loss of mass. These results, although incipient, are useful because they can contribute to food safety, in addition to determining new ways of preserving Persea americana and being able to market it as a fourth-range product.

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