scholarly journals Extracts of Poplar Buds (Populus balsamifera L., Populus nigra L.) and Lithuanian Propolis: Comparison of Their Composition and Biological Activities

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 828
Author(s):  
Monika Stanciauskaite ◽  
Mindaugas Marksa ◽  
Mindaugas Liaudanskas ◽  
Liudas Ivanauskas ◽  
Marija Ivaskiene ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Populus Nigra ◽  
Raw Material ◽  
Populus Balsamifera ◽  
Plant Materials ◽  
Antioxidant Power ◽  
Balsam Poplar ◽  
Black Poplar

Balsam poplar and black poplar (Populus balsamifera L. and Populus nigra L.) buds that grow in Lithuania are the primary source of propolis, therefore it is proper to evaluate and compare the composition of these raw plant materials and propolis quantitatively and qualitatively. Propolis and balsamic poplar bud extract are dominated by p-coumaric acid and black poplar-caffeic acid. Antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (ferric-reducing antioxidant power) and CUPRAC (cupric reducing antioxidant capacity) methods and all extracts showed antioxidant activity, and obtained results correlated with the obtained amounts of phenolic compounds and flavonoids in the extracts. Studies of antimicrobial activity have shown that all extracts have a growth inhibitory effect against Staphylococcus aureus and Candida albicans, but the extract of balsam poplar buds showed the most significant effect of such kind. Considering the results of the research, it can be stated that balsam poplar buds cultured in Lithuania are the primary raw material of propolis, which is rich in phenolic compounds with antioxidant properties and is a promising raw material for pharmaceutical purposes.

scholarly journals Comparison of Ethanolic and Aqueous Populus balsamifera L. Bud Extracts by Different Extraction Methods: Chemical Composition, Antioxidant and Antibacterial Activities

2021 ◽  
Vol 14 (10) ◽  
pp. 1018
Author(s):  
Monika Stanciauskaite ◽  
Mindaugas Marksa ◽  
Lina Babickaite ◽  
Daiva Majiene ◽  
Kristina Ramanauskiene
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antibacterial Activities ◽  
Extraction Methods ◽  
Raw Material ◽  
Total Phenolic ◽  
Populus Balsamifera ◽  
Antioxidant Power ◽  
Balsam Poplar ◽  
Activity Effect

The balsam poplar (Populus balsamifera L.) buds that grow in Lithuania are a polyphenol-rich plant material with a chemical composition close to that of propolis. In order to potentially adapt the extracts of this plant’s raw material for therapeutic purposes, it is important to carry out detailed studies on the chemical composition and biological activity of balsam poplar buds. An important step is to evaluate the yield of polyphenols by different extraction methods and using different solvents. According to our research, extracts of balsam poplar buds collected in Lithuania are dominated by p-coumaric (496.9–13,291.2 µg/g), cinnamic acid (32.9–11,788.5 µg/g), pinobanksin (34.9–1775.5 µg/g) and salicin (215.3–1190.7 µg/g). The antioxidant activity of poplar buds was evaluated by the ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric-reducing antioxidant power) methods, all extracts showed antioxidant activity and the obtained results correlated with the obtained amounts of total phenolic compounds in the extracts (ABTS r = 0.974; DPPH r = 0.986; FRAP r = 0.955, p < 0.01). Studies of antimicrobial activity have shown that ethanolic extracts have an antimicrobal activity effect against Staphylococcus aureus, Enterococcus faecalis and Escherichia coli. The extracts showed a better antimicrobal activity against gram-positive bacteria.

scholarly journals Antioxidant and Antibacterial Activity of Roseroot (Rhodiola rosea L.) Dry Extracts

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1767 ◽  
Author(s):  
Olga Kosakowska ◽  
Katarzyna Bączek ◽  
Jarosław L. Przybył ◽  
Ewelina Pióro-Jabrucka ◽  
Weronika Czupa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Rhodiola Rosea ◽  
Array Detector ◽  
Chemical Compositions ◽  
Antioxidant Power ◽  
Scavenging Capacity ◽  
Chemical Profiles

Roseroot (Rhodiola rosea L.) belongs to plants revealing adaptogenic properties, which are attributed to the presence of specific phenolic compounds and are reflected mainly as antioxidant activity. The aim of the present study was to determine the antioxidant and antibacterial activity of various products obtained from R. rosea (underground organs as well as their aqueous and ethanolic dry extracts) in relation to the chemical profiles of phenolic and essential oil compounds. The chemical profiles were determined by High-performance Liquid Chromatography with a diode-array detector (HPLC-DAD) and Gas chromatography-mass spectrometry (GC-MS), antioxidant activity by (1,1-Diphenyl-2-picrylhydrazyl) Scavenging Capacity Assay (DPPH), (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) Scavenging Capacity Assay (ABTS) and Ferric Reducing Antioxidant Power Assay (FRAP) and antimicrobial properties were expressed as minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) values following the broth microdilutions method. The results show that the investigated samples differed in terms of their chemical compositions and biological activities. The extracts were more abundant in phenolic compounds (salidroside, tyrosol, and rosavin derivatives) in comparison to dried underground organs. The content of the determined phenolics in the analyzed extracts was affected by the solvent used for extraction. The ethanolic extract was characterized by the highest content of these substances in comparison to the aqueous one and the dried raw material, especially with regard to rosavin (969.71 mg/100 g). In parallel, this extract showed the strongest antioxidant and antibacterial activity. However, dried R. rosea underground organs also revealed strong antibacterial effects against, for example, Staphylococcus strains.

scholarly journals The study of the composition of phenolic compounds of the populus nigra leaves

2020 ◽  
Vol 19 (5-6) ◽  
pp. 128-134
Author(s):  
Elena A. Kupriyanova ◽  
Vladimir A. Kurkin
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Compounds ◽  
Nmr Spectroscopy ◽  
13C Nmr ◽  
13C Nmr Spectroscopy ◽  
Populus Nigra ◽  
Raw Material ◽  
Black Poplar ◽  
Phenolic Components ◽  
First Time

This paper presents the findings of the study of the composition of phenolic compounds in black poplar leaves (Populus nigra L.). For the first time flavonoid compounds such as сalendoflavobioside (quercetin-3-O-neohesperidoside), rutin (quercetin-3-O-rutinoside), and ferulic acid (phenylpropanoid) have been extracted from the leaves of the black poplar by the methods of extraction and the following adsorption column chromatography on silica gel and on polyamide sorbent. The obtained substances were characterized with the use of UV-, 1H-NMR-, 13C-NMR-spectroscopy and mass spectrometry and also by means of the results of acid hydrolysis. The flavonoids are the dominant phenolic components of the raw material of this plant.

scholarly journals Content of Phenolic Compounds in Various Sea Buckthorn Parts

2013 ◽  
Vol 67 (4-5) ◽  
pp. 411-415 ◽  
Author(s):  
Elga Šne ◽  
Dalija Segliņa ◽  
Ruta Galoburda ◽  
Inta Krasnova
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sea Buckthorn ◽  
Raw Material ◽  
Antioxidant Power ◽  
Spectrophotometric Methods ◽  
Ferric Reducing Antioxidant Power ◽  
Medicinal Properties

Abstract All parts of the sea buckthorn (Hippophae rhamnoides L.) plant are considered to contain large amounts of compounds that are believed to have beneficial health effects. Till now, different parts of sea buckthorn plant have been used for the treatment of diseases in traditional medicine in various countries. Nevertheless, sea buckthorn parts would be a good raw material not only for medicinal properties but also for food products with functional properties; therefore, the aim of the research was to determine the concentration of different phenolic compounds and antioxidant activity in various sea buckthorn parts. The study was conducted on parts of female and male sea buckthorn bushes. Phenolic compounds (total phenols, total flavonoids and condensed tannins) and antioxidative activity (ferric reducing antioxidant power (FRAP) free radical scavenging activity (using 2.2-diphenyl-1-picrylhydrazyl, DPPH)) in ethanolic extracts of leaves, shoots, flowers, and berries were determined using various spectrophotometric methods. The study showed that concentration of phenolic compounds differed among parts of sea buckthorn plant and among gender. Leaves of female plants proved to be the most valuable, as they contained the highest total phenol concentration (165.76 mg/g) and antioxidant activity (220.97 mg/g for FRAP and 43.76 mg/g for DPPH), while lowest values were found in young shoots of male plants (84.94 mg/g, 94.24 mg/g and 24.63 mg/g, respectively). The significant differences in chemical composition and biological activity of sea buckthorn leaves, shoots, berries, and buds indicate a need for detailed studies of their extracts, specific fractions and compounds during a whole vegetative season.

scholarly journals Optimization of Ultrasonic Extraction of Phenolic Compounds fromEpimedium brevicornumMaxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Yan Zhao ◽  
Yingying Hou ◽  
Guosheng Tang ◽  
Enbo Cai ◽  
Shuangli Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Extraction Yield ◽  
Raw Material ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power

The ultrasound-assisted extraction of phenolic compounds fromEpimedium brevicornuMaxim was modeled using response surface methodology. A Central Composite Design (CCD) was employed to optimize three extraction variables, including ethanol concentration (X1), extraction time (X2), and ratio of aqueous ethanol to raw material (X3), for the achievement of high extraction yield of the phenolic compounds. The optimized conditions areX1of 50% (v/v),X2of 27.5 min, andX3of 250 mL/g. Under these conditions, the experimental yield is 4.29 ± 0.033%(n=3). The antioxidant activity was evaluated using the DPPH assay and ferric-reducing antioxidant power (FRAP). And it indicates that the phenolic compounds fromEpimedium brevicornuMaxim possess significant antioxidant activity. HPLC analysis reveals that the main phenolic compound in the extract product was identified as gallic acid, catechin (Cianidanol), p-hydroxybenzoic acid, vanillic acid, caffeic acid, ferulaic acid, rutin, benzoic acid, and quercetin.

scholarly journals Phenolic Compounds and Antioxidant Potential of Wild-Growing Plant Materials of the North Caucasus Region

2019 ◽  
Vol 9 (2) ◽  
pp. 5062-5071 ◽  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Raw Materials ◽  
Functional Materials ◽  
Total Content ◽  
Raw Material ◽  
North Caucasus ◽  
Plant Materials ◽  
The North ◽  
Quantitative Content

The article presents the results of studying the qualitative composition and quantitative content of some groups of phenolic compounds in 11 types of medicinal plants growing in the foothills of the North Caucasus, and provides the estimates of the antioxidant activity of extracts from these plants. The qualitative and quantitative content of phenolic compounds was determined using a Kapel-105M capillary electrophoresis system, and the total antioxidant activity of the extracts was measured on a Tsvet Yauza-01-AA device with an amperometric detector. In the studied plant samples, the total content of tannins was determined, eight phenolcarbonic acids were identified and quantified, as well as quercetin and rutin — two of the most important flavonols. The highest total content of phenolcarbonic acids (11,776.2 mg/kg), as well as the highest antioxidant activity were noted in the aqueous extract obtained from Echinacea purpurea (lat. Echinacea angustifolia). The direct relationship between the antioxidant activity of the studied medicinal raw material and the content of phenolic compounds has been experimentally established as follows: the higher is the concentration of phenolic substances, the higher is the antioxidant activity. The results of this study provide new information on the composition and content of phenolic compounds in some types of wild-growing plant raw materials of the North Caucasus and the antioxidant activity of extracts based thereon that will facilitate the use of the studied plants as a potential source of natural antioxidants in the production of functional materials.

scholarly journals Antioxidant properties, quantification and stability of betalains from pitaya (Hylocereus undatus) peel

2014 ◽  
Vol 45 (2) ◽  
pp. 323-328 ◽  
Author(s):  
Fernanda Robert de Mello ◽  
Claudia Bernardo ◽  
Caroline Odebrecht Dias ◽  
Luciano Gonzaga ◽  
Edna Regina Amante ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Raw Material ◽  
High Concentration ◽  
Natural Colorant ◽  
Wide Ph Range ◽  
Hylocereus Undatus ◽  
Ph Range ◽  
Promising Source

Pitaya peel can be used as a raw material for betalains extraction. The aim of this research was to quantify phenolic compounds, antioxidant activity and betalains on pitaya peel. Furthermore, evaluate the betalains stability against various pH conditions and exposure time of heating. The results showed that pitaya peel contains phenolic compounds and presented antioxidant activity. Moreover it showed high concentration of betalains (101.04mg equivalent to betanin. 100g-1) which were stable over a wide pH range (3.2 - 7.0) and were resistant to heating (100oC) up to 10 minutes at pH range from 3.7 to 5.5. Therefore, pitaya peel is a promising source of betalains which can be applied as a natural colorant for food.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

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