Biopriming with Seaweed Extract and Microbial-Based Commercial Biostimulants Influences Seed Germination of Five Abelmoschus esculentus Genotypes

Seed germination is a crucial step in plant propagation, as it controls seedling production, stand establishment and ultimately crop yield. Approaches that can promote seed germination of valuable crops remain of great interest globally. The current study evaluated the effect of biostimulant (Kelpak® and plant-growth-promoting rhizobacteria—PGPR) biopriming on the seed germination of five (VI037996, VI046567, VI055421, VI050956, and VI033796) Abelmoschus esculentus genotypes. The germination responses of the bio-primed seeds were measured using six parameters, including final germination percentage (FGP), mean germination time (MGT), germination index (GI), coefficient of velocity of germination (CVG), germination rate index (GRI), and time spread of germination (TSG). Biostimulant application significantly affected MGT (1.1–2.2 days), CVG (1.4–5.9), and TSG (1.2–3.0 days). Genotype also significantly influenced the TSG (1–3 days). Significant interaction effect of biostimulant treatment and genotype was evident on the FGP, GI, and GRI of the germinated seeds. The most noteworthy effect was demonstrated by Kelpak® (1:100) applied to genotype VI037996, with significantly improved FGP (82%), GI (238), and GRI (77%/day) when compared to the control. Overall, the current findings suggest the potential stimulatory effect of biostimulants (especially Kelpak®) on the germination of Abelmoschus esculentus seeds. However, this influence was strongly dependent on the type of genotype.

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Effect of seed treatments and potting medium on seed germination parameters in threatened Stereospermum suaveolens (Roxb.) DC. – A dashmool species

Environment Conservation Journal ◽

10.36953/ecj.2020.211225 ◽

2020 ◽

Vol 21 (1&2) ◽

pp. 187-192

Author(s):

Hariom Saxena ◽

Naseer Mohammad ◽

Samiksha Parihar ◽

Sant Kumar

Keyword(s):

Seed Germination ◽

Seed Treatment ◽

Germination Rate ◽

Germination Percentage ◽

Hot Water ◽

Potting Medium ◽

Potting Media ◽

Mean Germination Time ◽

Coefficient Of Velocity ◽

Experimental Treatments

An experiment was conducted to investigate the effect of different seed treatment and potting media on germination percentage and associate parameters in Stereospermum suaveolens, a threatened dashmool species during May, 2019 at Silviculture Nursery of Tropical Forest Research Institute, Jabalpur (Madhya Pradesh). Experimental treatments comprised of twenty treatment combinations consisting of four different seed treatment and five growth mediums. Data on daily seed germination was recorded and used for estimation of final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG), germination rate index (GRI), time spread of germination (TSG), energy period (EP) and peak value of mean germination (PVMG). Critical perusal of analysis revealed that treatment combination ‘Sand + Hot Water’ was resulted in positive effect on all the characters studied and could be used for mass raising of seedlings of S. suaveolens.

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Morphological and germination response variability in seeds of wild yellow gentian (Gentiana lutea L.) accessions from northwest Spain

Botany ◽

10.1139/b2012-028 ◽

2012 ◽

Vol 90 (8) ◽

pp. 731-742 ◽

Cited By ~ 8

Author(s):

Félix Pérez-García ◽

Federico Varela ◽

M. Elena González-Benito

Keyword(s):

Seed Germination ◽

Water Content ◽

Germination Rate ◽

Seed Mass ◽

Germination Percentage ◽

Gentiana Lutea ◽

Moist Chilling ◽

Mean Germination Time ◽

Wild Accessions ◽

Seed Water Content

Gentiana lutea L. (yellow gentian, Gentianaceae) is an important medicinal plant under protection as endangered species in most European countries. The aim of this work was to evaluate variation in seed mass, seed water content, and seed germination among 56 wild accessions of G. lutea. The effect of gibberellic acid (GA3), putrescine, moist chilling, and level of ripeness of seeds on subsequent germination was also investigated. Seeds of G. lutea showed physiological dormancy (final germination percentages ranged from 0% to 11%, depending on the accession) and GA3 enhanced seed germination drastically in all the accessions. The highest germination (99%) of GA3-treated seeds was reached at 15 °C. Final germination percentage and germination rate (as expressed by mean germination time), as well as seed mass and seed water content, varied significantly among accessions. In general, 1 year moist chilling did not significantly enhance G. lutea seed germination. For most accessions, no significant differences were found between fully ripe seeds and less ripe seeds for seed water content, seed mass, and seed germination. Applications of GA3 were always most effective than those of putrescine for increasing seed germination.

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The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity Stress

Vegetable Crops Research Bulletin ◽

10.2478/v10032-012-0008-z ◽

2012 ◽

Vol 76 (1) ◽

pp. 119-126 ◽

Cited By ~ 8

Author(s):

Maryam Haghighi ◽

Zahra Afifipour ◽

Maryam Mozafarian

Keyword(s):

Seed Germination ◽

Salinity Stress ◽

Seedling Growth ◽

Germination Rate ◽

Germination Percentage ◽

Percentage Germination ◽

Tomato Seeds ◽

Germination Characteristics ◽

Salinity Levels ◽

Mean Germination Time

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.

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Germination Response of MR 219 Rice Variety to Different Exposure Times and Periods of 2450 MHz Microwave Frequency

The Scientific World JOURNAL ◽

10.1155/2013/408026 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

Daryush Talei ◽

Alireza Valdiani ◽

Mahmood Maziah ◽

Mohammad Mohsenkhah

Keyword(s):

Seed Germination ◽

Root Length ◽

Germination Rate ◽

Rice Variety ◽

Microwave Frequency ◽

Germination Percentage ◽

Rice Seed ◽

Germination Time ◽

Primary Shoot ◽

Mean Germination Time

Germination is a key process in plants' phenological cycles. Accelerating this process could lead to improvment of the seedling growth as well as the cultivation efficiency. To achieve this, the effect of microwave frequency on the germination of rice seeds was examined. The physiological feedbacks of the MR 219 rice variety in terms of seed germination rate (GR), germination percentage (GP), and mean germination time (MGT) were analyzed by exposing its seeds to 2450 MHz of microwave frequency for one, four, seven, and ten hours. It was revealed that exposing the seeds to the microwave frequency for 10 hours resulted in the highest GP. This treatment led to 100% of germination after three days with a mean germination time of 2.1 days. Although the other exposure times of microwave frequency caused the moderate effects on germination with aGPa3ranged from 93% to 98%, they failed to reduce theMGTa3. The results showed that ten-hour exposure times of microwave frequency for six days significantly facilitated and improved the germination indices (primary shoot and root length). Therefore, the technique is expected to benefit the improvement of rice seed germination considering its simplicity and efficacy in increasing the germination percentage and rate as well as the primary shoot and root length without causing any environmental toxicity.

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Germination of Primed Seed under NaCl Stress in Wheat

ISRN Botany ◽

10.5402/2012/167804 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Michael P. Fuller ◽

Jalal H. Hamza ◽

Hail Z. Rihan ◽

Mohammad Al-Issawi

Keyword(s):

Salt Stress ◽

Germination Rate ◽

Arable Land ◽

Nacl Stress ◽

Germination Percentage ◽

Randomized Design ◽

Nacl Concentration ◽

Mean Germination Time ◽

Coefficient Of Velocity ◽

Germination Indices

Soil salinity affects a large and increasing amount of arable land worldwide, and genetic and agronomic solutions to increasing salt tolerance are urgently needed. Experiments were conducted to improve wheat seed performance under salinity stress conditions after priming. An experiment was conducted using a completely randomized design of four replications for germination indices in wheat (Triticum aestivum L. cv. Caxton). Normal and primed seed with PEG6000 at MPa and five concentrations of NaCl (0, 50, 100, 150, and 200 mM) were tested. Results indicate that priming seed significantly () increased germination percentage at first count and final count, coefficient of velocity of germination, germination rate index, and mean germination time, while increasing of NaCl concentration significantly reduced it. Priming seed improved germination attributes at all NaCl concentration levels. The priming appeared to be able to overcome the effect of salt stress at 50 to 100 mM and reduce the effect of NaCl at higher concentrations up to 200 mM. The primed seed gave both faster germination and led to higher germination when under salt stress. We conclude that using priming techniques can effectively enhance the germination seed under saline condition.

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Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

Agronomía Colombiana ◽

10.15446/agron.colomb.v33n3.53074 ◽

2016 ◽

Vol 34 (1) ◽

pp. 17-24 ◽

Cited By ~ 2

Author(s):

Fabio Ernesto Martínez M. ◽

Diego Miranda L. ◽

Stanislav Magnitskiy

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Positive Response ◽

Germination Rate ◽

Germination Percentage ◽

Annona Squamosa ◽

Germination Time ◽

Mean Germination Time ◽

Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

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Effect of salinity on seed germination of some tomato (Lycopersicon esculentum Mill.) varieties

Journal of Aridland Agriculture ◽

10.25081/jaa.2021.v7.6588 ◽

2021 ◽

pp. 76-82

Author(s):

Getachew Shumye Adilu ◽

Yohannes Gedamu Gebre

Keyword(s):

Seed Germination ◽

Abiotic Factors ◽

Germination Rate ◽

Petri Dish ◽

Germination Percentage ◽

Control Treatment ◽

Randomized Design ◽

Salinity Levels ◽

Mean Germination Time ◽

And Control

Salinity adversely affects 20-30% of the irrigated area in the world. Tomato is sensitive to salinity. It is one of the most severe abiotic factors of many agricultural crops and it becoming the main problem in Ethiopia. This study was conducted to evaluate the effects of different salinity levels on the seed germination parameters of tomato varieties. It was laid out in a completely randomized design with three replicates. The treatment included four tomato varieties (Sirinka, Weyno, ARP D2, and Roma VF) and five salinity levels (1 dS m-1, 2 dS m-1, 3 dS m-1, 4 dS m-1, and control). Fifty seeds were placed in a Petri dish over a moistened germination paper for germination and seedlings and allowed to grow for 14 days. The germination rate, speed and energy of tomato seeds were significantly (p < 0.001) affected by the combined effect of variety and salinity. The shortest mean germination time, the highest mean germination rate, and the highest speed of germination were recorded in the ARP D2 variety in the control treatment. The lowest first and last days of germination, and the uncertainty of germination were recorded from ARP D2. However, an increase in the days of germination and in the uncertainty of germination, and a decrease in the germination index and total germination percentage trends were observed with increasing salinity levels. The highest level of salinity (4 dS m-1) affected the germination of tomato varieties. Among the four tested tomato varieties, ARP D2 and Roma VF were tolerant to salinity.

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Problems with using mean germination time to calculate rate of seed germination

Australian Journal of Botany ◽

10.1071/bt15133 ◽

2015 ◽

Vol 63 (8) ◽

pp. 631 ◽

Cited By ~ 39

Author(s):

Elias Soltani ◽

Farshid Ghaderi-Far ◽

Carol C. Baskin ◽

Jerry M. Baskin

Keyword(s):

Germination Rate ◽

Germination Percentage ◽

The Other ◽

Seed Vigour ◽

Or Groups ◽

Germination Speed ◽

Mean Germination Time ◽

Treatment Comparisons ◽

Time Spread ◽

Mean Time

Seed scientists and other plant biologists are interested in the measurement of germination because seeds from different individuals, populations, seed lots and treatments can differ in germination percentages, rate (speed) and uniformity. Mean time to germination (MGT) is a measure of the rate and time-spread of germination; however, there is a problem with using this method to calculate germination rate. MGT does not show the time from the start of imbibition to a specific germination percentage. MGT has been used to compare specific pairs or groups of means and to evaluate seed vigour. However, it is not the real time to mean germination but just an index of germination speed. Using MGT is not correct for ANOVA, post-ANOVA or the other comparison tests, because it does not show time to a specific germination percentage. Thus, we recommend that t50 be used instead of MGT. The t50 has all benefits of MGT, but it does not have the problems of MGT in treatment comparisons.

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SEED GERMINATION AND SEEDLING ESTABLISHMENT OF CHERIMOYA (ANNONA CHERIMOLA MILL.) AT DIFFERENT TEMPERATURES

Cercetari Agronomice in Moldova ◽

10.46909/cerce-2020-016 ◽

2020 ◽

Vol 53 (2) ◽

pp. 185-194

Author(s):

A. KHELOUFI ◽

L. M. MANSOURI ◽

H. KHETTACHE

Keyword(s):

Seed Germination ◽

Room Temperature ◽

Germination Percentage ◽

Annona Cherimola ◽

Seedling Length ◽

Different Temperatures ◽

External Conditions ◽

Mean Germination Time ◽

Coefficient Of Velocity ◽

Kinetics Of

Cherimoya (Annona cherimola Mill.) has an exceptional flavor and aroma, which makes it a fruit with great potential. However, little is known about its propagation by seeds. According to the scientific literature, the germination of cherimoya seeds is affected much more by external conditions than by internal conditions. Germination of cherimoya variety ‘Concha Lisa’ were tested for germination at constant temperatures of 25, 30, 40°C, and at room temperature, varying from 20-25°C, coupled with total darkness. Seeds were sown in Petri dishes (0.8% agar water), for 25 days of incubation. The kinetics of germination was determined according to five closely related parameters, viz. final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG), time to 50% germination (T50) and seedling length (SL). The temperature of 30°C was found optimally suitable with 70.8% FGP, 17.5 days MGT and 3.91 cm SL, while the room temperature of 20-25°C slightly improved germination with only 25% FGP. Furthermore, significant decrease in FGP and SL was observed at 25°C and 40°C of temperature in comparison to 30°C. The analysis also revealed that cherimoya seed germination, day 10-15 after seed sowing is suitable for final counts. An overview on the emergence of cherimoya seedlings, during a 12-week period in pots is presented.

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The effect of plant growth promoting rhizobacteria on Datura stramonium L., Abutilon theophrasti Med., Onopordon acanthium L. and Verbascum thapsus L. seed germination

Pesticidi i fitomedicina ◽

10.2298/pif1403205b ◽

2014 ◽

Vol 29 (3) ◽

pp. 205-212 ◽

Cited By ~ 1

Author(s):

Dragana Bozic ◽

Ljubinko Jovanovic ◽

Vera Raicevic ◽

Danijela Pavlovic ◽

Marija Saric-Krsmanovic ◽

...

Keyword(s):

Seed Germination ◽

Plant Growth Promoting Rhizobacteria ◽

Datura Stramonium ◽

Germination Percentage ◽

Abutilon Theophrasti ◽

Weed Species ◽

Verbascum Thapsus ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Petri Dishes

The effects of several bacterial media [Bacillus licheniformis population 1 (MO1); B. licheniformis population 2 (MO2); B. subtilis (MO3); B. megatherium (MO4); humates (MO5)] on seed germination of Datura stramonium L., Abutilon theophrasti Med., Onopordon acanthium L. and Verbascum thapsus L. were tested. Seeds were germinated in Petri dishes containing solutions with different bacterial media. The highest germination percentage in all treatments was recorded for V. thapsus seeds (100.0%). Different treatments had diverse effects (stimulative or inhibitory) on seed germination of D. stramonium [from 5% (MO1) to 13.3% (MO3), with 10.0 % in H20], A. theophrasti [from 28.3% (MO3) to 65.0% (MO5), with 43.3 % in H20] and O. acanthium [from 10.0% (MO2) to 13.3% (MO1 and MO3), with 6.7% in H20], depending on the type of media and weed species.

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