scholarly journals Diversity Assessment of the Montenegrin Maize Landrace Gene Pool Maintained in Two Gene Banks

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1503
Author(s):  
Vojka Babić ◽  
Violeta Andjelkovic ◽  
Zoran Jovovic ◽  
Milosav Babic ◽  
Vladimir Vasic ◽  
...  
Keyword(s):  
Gene Pool ◽  
Sustainable Use ◽  
Separate Analysis ◽  
Maize Breeding ◽  
Gene Banks ◽  
Diversity Assessment ◽  
Yellow Orange ◽  
Breeding Programmes ◽  
Maize Landraces ◽  
Joint Monitoring

Due to the loss of agro-biodiversity, there is a strong effort to find apparent and efficient mechanisms for the conservation and sustainable use of genetic diversity. A joint monitoring of the diversity and collections structure of the Montenegrin maize landraces conserved in the Serbian (MRIZPGB) and Montenegrin (MGB) gene banks has been conducted in order to improve the composition of the collections and to identify and eliminate possible redundancy. Based on a separate analysis of white- and yellow-orange maize landraces, it can be concluded that the diversity and evolution of distinct maize landraces grown and collected in Montenegro have been simultaneously shaped by both environmental (i.e., natural selection) and socially driven factors (farmers’ selection, migration and colonization processes of the human population). Although it has been determined that the authenticity and variability of the Montenegrin maize landraces gene pool have largely been preserved in the MRIZPGB collection, a significant amount of redundancy was observed. The obtained results will contribute to the cost-efficient conservation of the maize gene pool in the Montenegrin and Serbian gene banks. The recognized and well-preserved original variability of the MRIZPGB and MGB Montenegrin gene pool represents a valuable source for pre-breeding activities on broadening the white and flint maize breeding programmes under temperate conditions.

Hybrid maize breeding with doubled haploids: Comparison between selection criteria

2006 ◽  
Vol 54 (3) ◽  
pp. 343-350 ◽  
Author(s):  
C. F. H. Longin ◽  
H. F. Utz ◽  
A. E. Melchinger ◽  
J.C. Reif
Keyword(s):  
Selection Criteria ◽  
Doubled Haploids ◽  
Optimum Allocation ◽  
Finite Sample ◽  
Sample Sizes ◽  
Maize Breeding ◽  
Selection Gain ◽  
Breeding Programmes ◽  
Hybrid Maize ◽  
Breeding Resources

The optimum allocation of breeding resources is crucial for the efficiency of breeding programmes. The objectives were to (i) compare selection gain ΔGk for finite and infinite sample sizes, (ii) compare ΔGk and the probability of identifying superior hybrids (Pk), and (iii) determine the optimum allocation of the number of hybrids and test locations in hybrid maize breeding using doubled haploids. Infinite compared to finite sample sizes led to almost identical optimum allocation of test resources, but to an inflation of ΔGk. This inflation decreased as the budget and the number of finally selected hybrids increased. A reasonable Pk was reached for hybrids belonging to the q = 1% best of the population. The optimum allocations for Pk(q) and ΔGkwere similar, indicating that Pk(q) is promising for optimizing breeding programmes.

Improvement of effectiveness in maize breeding

2006 ◽  
Vol 54 (3) ◽  
pp. 351-358 ◽  
Author(s):  
P. Pepó
Keyword(s):  
Recurrent Selection ◽  
Genetic Manipulation ◽  
Field Testing ◽  
Tissue Cultures ◽  
Maize Breeding ◽  
Breeding Programmes ◽  
Speed Up ◽  
Selection For

Plant regeneration via tissue culture is becoming increasingly more common in monocots such as maize (Zea mays L.). Pollen (gametophytic) selection for resistance to aflatoxin in maize can greatly facilitate recurrent selection and the screening of germplasm for resistance at much less cost and in a shorter time than field testing. In vivo and in vitro techniques have been integrated in maize breeding programmes to obtain desirable agronomic attributes, enhance the genes responsible for them and speed up the breeding process. The efficiency of anther and tissue cultures in maize and wheat has reached the stage where they can be used in breeding programmes to some extent and many new cultivars produced by genetic manipulation have now reached the market.

Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
R. K Khulbe ◽  
A. Pattanayak ◽  
Lakshmi Kant ◽  
G. S. Bisht ◽  
M. C. Pant ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Sweet Corn ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Line Production ◽  
Haploid Inducer ◽  
Breeding Programmes ◽  
Source Populations ◽  
Developmental Phases

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

scholarly journals Molecular characterization and genetic diversity studies of Indian soybean (Glycine max (L.) Merr.) cultivars using SSR markers

2021 ◽  
Author(s):  
S. P. Jeevan Kumar ◽  
C. Susmita ◽  
K. V. Sripathy ◽  
Dinesh K. Agarwal ◽  
Govind Pal ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Ssr Markers ◽  
Crop Yield ◽  
Gene Pool ◽  
Stress Factors ◽  
Soybean Cultivars ◽  
Genetic Base ◽  
Purity Analysis ◽  
Breeding Programmes

Abstract Background The genetic base of soybean cultivars in India has been reported to be extremely narrow, due to repeated use of few selected and elite genotypes as parents in the breeding programmes. This ultimately led to the reduction of genetic variability among existing soybean cultivars and stagnation in crop yield. Thus in order to enhance production and productivity of soybean, broadening of genetic base and exploring untapped valuable genetic diversity has become quite indispensable. This could be successfully accomplished through molecular characterization of soybean genotypes using various DNA based markers. Hence, an attempt was made to study the molecular divergence and relatedness among 29 genotypes of soybean using SSR markers. Methods and results A total of 35 SSR primers were deployed to study the genetic divergence among 29 genotypes of soybean. Among them, 14 primer pairs were found to be polymorphic producing a total of 34 polymorphic alleles; and the allele number for each locus ranged from two to four with an average of 2.43 alleles per primer pair. Polymorphic information content (PIC) values of SSRs ranged from 0.064 to 0.689 with an average of 0.331. The dendrogram constructed based on dissimilarity indices clustered the 29 genotypes into two major groups and four sub-groups. Similarly, principal coordinate analysis grouped the genotypes into four major groups that exactly corresponded to the clustering of genotypes among four sub-groups of dendrogram. Besides, the study has reported eight unique and two rare alleles that could be potentially utilized for genetic purity analysis and cultivar identification in soybean. Conclusion In the present investigation, two major clusters were reported and grouping of large number of genotypes in each cluster indicated high degree of genetic resemblance and narrow genetic base among the genotypes used in the study. With respect to the primers used in the study, the values of PIC and other related parameters revealed that the selected SSR markers are moderately informative and could be potentially utilized for diversity analysis of soybean. The clustering pattern of dendrogram constructed based on SSR loci profile displayed good agreement with the cultivar’s pedigree information. High level of genetic similarity observed among the genotypes from the present study necessitates the inclusion of wild relatives, land races and traditional cultivars in future soybean breeding programmes to widen the crop gene pool. Thus, hybridization among diverse gene pool could result in more heterotic combinations ultimately enhancing genetic gain, crop yield and resistance to various stress factors.

scholarly journals Gene banks for wild and cultivated sunflower genetic resources

OCL ◽  
2020 ◽  
Vol 27 ◽  
pp. 9 ◽  
Author(s):  
Sreten Terzić ◽  
Marie-Claude Boniface ◽  
Laura Marek ◽  
Daniel Alvarez ◽  
Karin Baumann ◽  
...  
Keyword(s):  
North America ◽  
Genetic Resources ◽  
Helianthus Annuus ◽  
Inbred Lines ◽  
Wild Sunflower ◽  
Gene Banks ◽  
Breeding Programmes ◽  
Wild Accessions ◽  
Modern Breeding ◽  
Local Research

Modern breeding of sunflower (Helianthus annuus L.), which started 100 years ago, increased the number and the diversity of cultivated forms. In addition, for more than 50 years, wild sunflower and other Helianthus species have been collected in North America where they all originated. Collections of both cultivated and wild forms are maintained in gene banks in many countries where sunflower is an important crop, with some specificity according to the availability of germplasm and to local research and breeding programmes. Cultivated material includes land races, open pollinated varieties, synthetics and inbred lines. The majority of wild accessions are ecotypes of wild Helianthus annuus, but also 52 other species of Helianthus and a few related genera. The activities of three gene banks, in USA, France and Serbia, are described in detail, supplemented by data from seven other countries. Past and future uses of the genetic resources for environmental adaptation and breeding are discussed in relation to genomic and improved phenotypic knowledge of the cultivated and wild accessions available in the gene banks.

scholarly journals Evidence of apple blotch resistance in wild apple germplasm (Malus spp.) accessions

2020 ◽  
Author(s):  
Thomas Wöhner ◽  
Ofere Francis Emeriewen ◽  
Monika Höfer
Keyword(s):  
Gene Pool ◽  
Field Survey ◽  
Recent Literature ◽  
Germplasm Collection ◽  
Economic Losses ◽  
Apple Breeding ◽  
High Interest ◽  
Breeding Programmes ◽  
Apple Cultivars ◽  
Inoculation Assay

AbstractDiplocarpon coronariae causes apple blotch, which results in significant economic losses. Resistance to apple blotch in commercial apple cultivars has not been reported in recent literature. The Malus germplasm collection from the Fruit Genebank of the Julius Kühn-Institut that is maintained in Dresden, Germany consists of 516 accessions of about 49 Malus species. This apple gene pool was evaluated for resistance to Diplocarpon coronariae. A four-year field survey was subsequently followed by artificial inoculation of field-selected accessions. Fifteen accessions that represent nine different species and hybrids were confirmed to be highly resistant following a repeated inoculation assay in the laboratory. The results from this investigation is of high interest to breeders for future apple breeding programmes and investigations of resistance to Diplocarpon coronariae.

scholarly journals Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 216-225 ◽  
Author(s):  
L. Havlíčková ◽  
E. Jozová ◽  
A. Rychlá ◽  
M. Klíma ◽  
V. Kučera ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Issr Markers ◽  
Appropriate Technology ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Genetic Diversity ◽  
Diversity Assessment ◽  
Breeding Programmes

The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100%, 53.9% and 90.6%, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3%) whereas significantly lower distances of 49.4% in SSRs and 35.5% in AFLPs were observed. The genetic similarity matrix clearly distinguished all accessions. A set of the most distinct varieties was established. The analysis of the genetic pattern of the accessions indicated two groups comprising most of the modern Czech breeding materials, revealing a distinct shift in breeding. Surprisingly, molecular analyses did not support  breeders’ views about a narrow genetic base of the Czech breeding materials. The choice of appropriate technology for different aspects of germplasm evaluation is also discussed.

scholarly journals Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
M. Govindaraj ◽  
M. Vetriventhan ◽  
M. Srinivasan
Keyword(s):  
Genetic Diversity ◽  
Food Production ◽  
Genetic Manipulation ◽  
Plant Genetic Resources ◽  
Crop Improvement ◽  
Genetic Material ◽  
Easy Access ◽  
Production Network ◽  
Gene Banks ◽  
Diversity Assessment

The importance of plant genetic diversity (PGD) is now being recognized as a specific area since exploding population with urbanization and decreasing cultivable lands are the critical factors contributing to food insecurity in developing world. Agricultural scientists realized that PGD can be captured and stored in the form of plant genetic resources (PGR) such as gene bank, DNA library, and so forth, in the biorepository which preserve genetic material for long period. However, conserved PGR must be utilized for crop improvement in order to meet future global challenges in relation to food and nutritional security. This paper comprehensively reviews four important areas; (i) the significance of plant genetic diversity (PGD) and PGR especially on agriculturally important crops (mostly field crops); (ii) risk associated with narrowing the genetic base of current commercial cultivars and climate change; (iii) analysis of existing PGD analytical methods in pregenomic and genomic era; and (iv) modern tools available for PGD analysis in postgenomic era. This discussion benefits the plant scientist community in order to use the new methods and technology for better and rapid assessment, for utilization of germplasm from gene banks to their applied breeding programs. With the advent of new biotechnological techniques, this process of genetic manipulation is now being accelerated and carried out with more precision (neglecting environmental effects) and fast-track manner than the classical breeding techniques. It is also to note that gene banks look into several issues in order to improve levels of germplasm distribution and its utilization, duplication of plant identity, and access to database, for prebreeding activities. Since plant breeding research and cultivar development are integral components of improving food production, therefore, availability of and access to diverse genetic sources will ensure that the global food production network becomes more sustainable. The pros and cons of the basic and advanced statistical tools available for measuring genetic diversity are briefly discussed and their source links (mostly) were provided to get easy access; thus, it improves the understanding of tools and its practical applicability to the researchers.

scholarly journals Genetic Variation for Biofortifying The Maize Grain

2016 ◽  
Vol 4 (8) ◽  
pp. 684 ◽  
Author(s):  
Gönül Cömertpay ◽  
Faheem Shehzad Baloch ◽  
Halil Erdem
Keyword(s):  
Starch Content ◽  
Principal Component ◽  
Mineral Elements ◽  
Quality Traits ◽  
Maize Breeding ◽  
Mineral Contents ◽  
Maize Germplasm ◽  
Geographical Regions ◽  
Maize Landraces ◽  
Maize Grain

The maize germplasm variation is valuable for breeders to develop elite hybrids with increased mineral contents in the maize grain to eliminate mineral malnutrition, which is referred as HIDEN HUNGER. Therefore, we aimed to determine mineral element diversity of maize landraces collected from different geographical regions of Turkey. There was huge diversity for all mineral traits and other quality traits. Turkish maize landraces showed high variation for Zn (17-41.34 mg kg-1), Fe (13.52-29.63 mg kg-1), Cu (0.77-3.34 mg kg-1), Mn (5.68-14.78 mg kg-1), Protein (6.6-11.6%), starch content (73.3-80.0%), oil content (3.15-4.7%) and thousand grain weight (177.0-374.9g). There were significant positive and negative associations among mineral elements and quality traits. The principal component analysis differentiated some maize landraces from the rest, and these diverse landraces could be used in the maize breeding program with biofortification purpose.

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