scholarly journals Anatomical and Chemical Characterization of Ulmus Species from South Korea

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2617
Author(s):  
Woo-Sung Park ◽  
Hye-Jin Kim ◽  
Atif Ali Khan Khalil ◽  
Dong-Min Kang ◽  
Kazi-Marjahan Akter ◽  
...  
Keyword(s):  
South Korea ◽  
High Performance ◽  
Folk Medicine ◽  
Chemical Characterization ◽  
Gastrointestinal Diseases ◽  
Array Detector ◽  
Cell Diameter ◽  
Root Bark ◽  
Orthogonal Projections ◽  
Latent Structures

Ulmus species (Ulmaceae) are large deciduous trees distributed throughout Korea. Although their root and stem bark have been used to treat gastrointestinal diseases and wounds in folk medicine, commercial products are consumed without any standardization. Therefore, we examined anatomical and chemical differences among five Ulmus species in South Korea. Transverse sections of leaf, stem, and root barks were examined under a microscope to elucidate anatomical differences. Stem and root bark exhibited characteristic medullary ray and secretary canal size. Leaf surface, petiole, and midrib exhibited characteristic inner morphologies including stomatal size, parenchyma, and epidermal cell diameter, as well as ratio of vascular bundle thickness to diameter among the samples. Orthogonal projections to latent structures discriminant analysis of anatomical data efficiently differentiated the five species. To evaluate chemical differences among the five species, we quantified (-)-catechin, (-)-catechin-7-O-β-D-apiofuranoside, (-)-catechin-7-O-α-L-rhamnopyranoside, (-)-catechin-7-O-β-D-xylopyranoside, (-)-catechin-7-O-β-D-glucopyranoside, and (-)-catechin-5-O-β-D-apiofuranoside using high-performance liquid chromatography with a diode-array detector. (-)-Catechin-7-O-β-D-apiofuranoside content was the highest among all compounds in all species, and (-)-catechin-7-O-α-L-rhamnopyranoside content was characteristically the highest in Ulmus parvifolia among the five species. Overall, the Ulmus species tested was able to be clearly distinguished on the basis of anatomy and chemical composition, which may be used as scientific criteria for appropriate identification and standard establishment for commercialization of these species

scholarly journals Comparative Inner Morphological and Chemical Studies on Reynoutria Species in Korea

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 222
Author(s):  
Atif Ali Khan Khalil ◽  
Kazi-Marjahan Akter ◽  
Hye-Jin Kim ◽  
Woo Sung Park ◽  
Dong-Min Kang ◽  
...  
Keyword(s):  
High Performance ◽  
Spectroscopic Studies ◽  
Array Detector ◽  
Vascular Bundles ◽  
Orthogonal Projections ◽  
Flowering Season ◽  
Projections To Latent Structures ◽  
Chemical Profiles ◽  
Chemical Studies ◽  
Latent Structures

Reynoutria species are medicinal plants that belong to the family Polygonaceae and are widely distributed in eastern Asia, North America and Europe. Although the phylogeny and morphological and anatomical studies of some species in Korea have been previously reported, there are no discriminative anatomical and chemical data available. Therefore, anatomical characterization of the leaf, stem and root, and high performance liquid chromatography–diode array detector (HPLC–DAD) analyses were carried out to assess the differences in anatomical and chemical profiles among the Reynoutria plants in Korea, i.e., R. japonica, R. sachalinensis, R. forbesii and R. japonica for. elata. The anatomical evaluation showed discriminative characteristics, such as the shape of the stomata and the stomatal index of the lower leaf surface; the ratio of the adaxial/abaxial height, the size of the vascular bundles and the frequency of druse in the midrib, petiole, and stem; and the pericycle number in the root. For the HPLC analysis, ten compounds corresponding to each major peak were isolated from R. japonica roots and their structures were identified by comprehensive spectroscopic studies. Samples collected before the flowering season showed higher contents of these ten major compounds than those collected after the flowering season. The orthogonal projections to latent structures-discrimination analysis (OPLS-DA) with the inner morphological and HPLC quantification results, clearly discriminated these plants. These results provide anatomical parameters and HPLC profiling that can be used to distinguish the four Reynoutria plants, which supports quality control for their precise identification.

scholarly journals Chemical characterization of an Ipecac active germplasm bank – a Brazilian endangered medicinal species

2021 ◽  
Vol 10 (15) ◽  
Author(s):  
Nádia Elígia Nunes Pinto Paracampo ◽  
Luiz Ricardo Santos de Souza ◽  
Jéssica Caporal Almeida ◽  
Osmar Alves Lameira
Keyword(s):  
High Performance ◽  
Genetic Material ◽  
Chemical Characterization ◽  
Array Detector ◽  
Germplasm Bank ◽  
Medicinal Species ◽  
Breeding Programs ◽  
The City ◽  
Selection Of

The active germplasm bank (AGB) of Carapichea ipecacuanha (Brot.) L. Andersson at Embrapa Eastern Amazon, in the city of Belém, PA, was the first of its kind to be opened in Brazil for this endangered medicinal species and holds important accessions for agricultural and reproductive research, including the production of active principles. This study aimed to chemically characterize 42 accessions from that AGB by simultaneously quantifying cephaeline and emetine contents in roots using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Cephaeline concentrations ranged from ‘undetected’ to 1.76%, whereas emetine concentrations were found between 0.64% and 2.49%. The overall emetine/cephaeline ratio varied from 0.43 to 3.52. The differences among mean concentrations of alkaloids observed by comparing the Scott-Knott test at 5% probability suggest the chemical variation among the samples assessed. Therefore, it is concluded such chemical differences may favor the selection of genetic material for commercial purposes based on the production of emetine and/or cephaeline, likewise may contribute to breeding programs.

scholarly journals Simultaneous Quantification of Two Flavonoids in Morus alba by High Performance Liquid Chromatography Coupled with Photodiode Array Detector

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301
Author(s):  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Morus Alba ◽  
Array Detector ◽  
Root Bark ◽  
Relative Standard ◽  
Photodiode Array

The root bark of Morus alba L. (Family: Moraceae) is an important medicinal herb in many countries and has long been used as a traditional medicine for the treatment of cough, fever, blood pressure reduction, and respiratory diseases. In the present study, the simultaneous determination of two flavonoids, kuwanon G and morusin, for quality control of M alba was conducted using high-performance liquid chromatography (HPLC) equipped with photodiode array (PDA) detector. The column used for separation of kuwanon G and morusin was a Gemini C18 analytical column maintained at 45°C. The mobile phase for efficient separation of two analytes was flowed 0.1% (v/v) aqueous formic acid-acetonitrile with gradient elution. The detection wavelength for quantification was set at 266 nm. The optimized method showed good linearity with coefficients of determination of 0.9998 within the tested concentration ranges. The limits of detection for the two flavonoids, kuwanon G and morusin, were 0.69 μg/mL and 0.35 μg/mL and the limits of quantification of kuwanon G and morusin, were 2.10 μ/mL and 1.07 μg/mL. The recoveries were 98.40–111.55% and the relative standard deviation (RSD) value was within 3.50%. The RSD values of intra- a g d interday precisions were 0.08–0.70% and 0.06-0.48%, respectively. The amounts of kuwanon G and morusin were 1.94-2.26 mg/g and 1.05–1.12 mg/g. The established HPLC-PDA method will help to improve the quality control of M. alba and related products.

scholarly journals The Influence of Anthocyanidin Profile on Antileishmanial Activity of Arrabidaea chica Morphotypes

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3547
Author(s):  
Carla Junqueira Moragas-Tellis ◽  
Fernando Almeida-Souza ◽  
Maria do Socorro dos Santos Chagas ◽  
Paulo Victor Ramos de Souza ◽  
João Victor Silva-Silva ◽  
...  
Keyword(s):  
High Performance ◽  
Folk Medicine ◽  
Principal Component ◽  
Statistical Correlation ◽  
Antileishmanial Activity ◽  
Leishmania Infection ◽  
Array Detector ◽  
Group I ◽  
Chemical Variability ◽  
Arrabidaea Chica

Arrabidaea chica Verlot (crajiru) is a plant used in folk medicine as an astringent, anti-inflammatory, wound healing and to treat fungal and viral diseases such as measles chickenpox and herpes. Arrabidaea chica has several morphotypes recognized but little is known about its chemical variability. In the present study the anthocyanidin profile of A. chica morphotypes collected in two seasons (summer and winter) have been examined and their activity against Leishmania infection compared. High-performance liquid chromatography coupled to a diode-array detector (HPLC-DAD-UV) and by tandem mass spectrometry with electrospray ionization (ESI-MS/MS) were used for anthocyanidin separation and identification. Antileishmanial activity was measured against promastigote forms of Leishmania amazonensis. Multivariate analysis, principal component analysis (PCA) and Pearson’s correlation were performed to classify morphotypes accordingly to their anthocyanidin profile. The presence of 6,7,3′,4′-tetrahydroxy-5-methoxyflavylium (3′-hydroxy-carajurone) (1), carajurone (2), 6,7,3′-trihydroxy-5,4′-dimethoxy-flavylium (3′-hydroxy-carajurin) (3) and carajurin (4), and three unidentified anthocyanidins were detected. Two different groups were recognized: group I containing 3′-hydroxy-carajurone; and group II with high content of carajurin. Among anthocyanidins identified in the extracts, only carajurin showed significant statistical correlation (p = 0.030) with activity against L. amazonensis. Carajurin could thus be considered as a pharmacological marker for the antileishmanial potential of the species.

scholarly journals Comparative Studies of Fraxinus Species from Korea Using Microscopic Characterization, Phytochemical Analysis, and Anti-Lipase Enzyme Activity

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 534
Author(s):  
Kazi-Marjahan Akter ◽  
Woo Sung Park ◽  
Hye-Jin Kim ◽  
Atif Ali Khan Khalil ◽  
Mi-Jeong Ahn
Keyword(s):  
Lipase Activity ◽  
High Performance ◽  
Folk Medicine ◽  
Lower Surface ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Orthogonal Projections ◽  
Cork Cell ◽  
Microscopic Evaluation ◽  
Chemical Profiles

Fraxinus species belongs to the Oleaceae family, commonly known as Ash tree, and has been utilized as a folk medicine with various medicinal properties, including anti-obesity activity. The goal of the present study was to establish quality control parameters using microscopic characterization, phytochemical differentiation, and anti-lipase activity evaluation of five Fraxinus plants in Korea. Microscopic evaluation of the lower surface, petiole, and midrib of leaves, and stem bark showed discriminative anatomical characteristics, such as the stomatal index of the lower leaf surface; the number of sclerenchyma cells, and the diameter of parenchyma cells in the petiole and midrib; and the cork cell size and fiber frequency in the stem bark. Phytochemical analysis using high-performance liquid chromatography revealed the significant variation in the chemical profiles of the 12 major secondary metabolites among the samples. The orthogonal projections to latent structure-discrimination analysis efficiently differentiated each group belonging to each Fraxinus plant with the anatomical and quantification data. F. rhynchophylla and ligstroside showed the most potent anti-lipase activity among the plants and the 12 major metabolites, respectively. These findings could serve as the scientific criteria for the appropriate identification and establishment of standards for the use of Fraxinus species as medicinal plants.

scholarly journals Comparison Study of Different Extracts of Plectranthus madagascariensis, P. neochilus and the Rare P. porcatus (Lamiaceae): Chemical Characterization, Antioxidant, Antimicrobial and Cytotoxic Activities

Biomolecules ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 179 ◽  
Author(s):  
Diogo Matias ◽  
Marisa Nicolai ◽  
Ana Sofia Fernandes ◽  
Nuno Saraiva ◽  
Joana Almeida ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Array Detector ◽  
Klebsiella Pneumonia ◽  
Cytotoxic Activities ◽  
Comparison Study ◽  
Activity Data

Medicinal plants of the Plectranthus genus (Lamiaceae) are known for their ethnopharmacological relevance, mainly against infectious, dermatologic and gastrointestinal pathologies. Three Plectranthus species originated from South Africa, namely P. madagascariensis, P. neochilus and the rare P. porcatus were hereby screened for their antimicrobial and cytotoxic activities related with their known and/or potential ethnomedicinal uses. Twenty-six extracts were prepared by the combination of extraction methods (infusion, decoction, microwave-assisted, ultrasound-assisted, maceration and supercritical fluid extraction) with different polarity solvents (water, methanol, acetone and supercritical CO2). The comparison study of these extracts was elucidated through the corresponding chemical characterization and cytotoxic activity data. Therefore, the acetone extract from P. madagascariensis prepared by ultrasound extraction method revealed potent antibacterial activity against Gram-positive bacteria (1.95 < minimum inhibitory concentration (MIC) < 7.81 μg/mL), including a methicillin-resistant Staphylococcus aureus (MRSA) strain. Additionally, acetone extracts from both P. madagascariensis and P. neochilus exhibited relevant antibacterial activity against Gram-negative Klebsiella pneumonia (0.48 < MIC < 3.91 μg/mL), validating the traditional uses of such plants as anti-infectious agents. All methanolic extracts showed potent antioxidant effects at 100 μg/mL measured as their radical scavenging activity (60.8–89.0%) in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The P. madagascariensis extract obtained by maceration in acetone showed moderate cytotoxic effects in the MDA-MB-231 cell line (triple negative human breast carcinoma). The extract concentration that caused a 50% inhibition in cell viability (IC50) was 64.52 μg/mL. All extracts in this comparative study were profiled by high-performance liquid chromatography-HPLC with a diode-array detector-DAD (HPLC-DAD) and the main known bioactive components were identified in each extract, which included polyphenols (caffeic 1, chlorogenic 2 and rosmarinic 3 acids), abietane diterpenes (7α-acetoxy-6β-hydroxyroyleanone 4 and coleon U 5) and flavone glycosides (rutin 6 and naringin 7).

scholarly journals Carotenoids profile and total polyphenols in fruits of Pereskia aculeata Miller

2012 ◽  
Vol 34 (1) ◽  
pp. 234-238 ◽  
Author(s):  
Tânia da Silveira Agostini-Costa ◽  
Danielle Cristina Wondraceck ◽  
Wesley da Silveira Rocha ◽  
Dijalma Barbosa da Silva
Keyword(s):  
High Performance ◽  
Chemical Characterization ◽  
Array Detector ◽  
Total Polyphenols ◽  
Bioactive Substances ◽  
Photodiode Array Detector ◽  
Tropical America ◽  
Photodiode Array ◽  
First Time

Pereskia aculeata Mill. (Ora-pro-nóbis) is a native cactaceae from tropical America, whose leaves have high protein content. In Brazil it is found in all territorial extension between the states of Bahia and Rio Grande do Sul. Most studies have focused on chemical characterization of the leaves of this specie. The objective was to assess the carotenoids profile and the total polyphenols present in the fruits of P. aculeate. Carotenoids were determined by HPLC-PAD (high performance liquid chromatography - photodiode array detector), total polyphenols were determined by Folin-Ciocalteu and vanillin methods. Trans-β-carotene was the main carotenoid, followed by α-carotene, lutein and other minor carotenoids. It was found 64.9 ± 1.1 mg.100g-1 of gallic acid equivalent, 14.8 ± 0.2 mg.100g-1 of catechin equivalent. Carotenoid identification of P. aculeate fruits are presented here by the first time and indicate that these fruits can be researched as source of bioactive substances, especially antioxidant and provitamin A carotenoids.

scholarly journals Protective Effect of Chemically Characterized Polyphenol-Rich Fraction from Apteranthes europaea (Guss.) Murb. subsp. maroccana (Hook.f.) Plowes on Carbon Tetrachloride-Induced Liver Injury in Mice

2021 ◽  
Vol 11 (2) ◽  
pp. 554
Author(s):  
Fatima Ez-Zahra Amrati ◽  
Mohammed Bourhia ◽  
Meryem Slighoua ◽  
Smahane Boukhira ◽  
Riaz Ullah ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
High Performance ◽  
Chemical Characterization ◽  
Array Detector ◽  
Biochemical Alterations ◽  
Traditional Medicines ◽  
Toxic Liver Injury ◽  
Promising Source

Apteranthes europaea (Guss.) Murb. subsp. maroccana (Hook.f.) Plowes (A. europaea) is a medicinal plant widely used in traditional medicines to treat various diseases including hepatic pathogenesis. This study was conducted to evaluate the protective effect of chemically characterized polyphenol-rich fraction from A. europaea on carbon tetrachloride-induced liver injury in mice. The chemical characterization of A. europaea polyphenol-rich fraction was carried out using HPLC-DAD (high-performance liquid chromatography (HPLC) with a diode-array detector (DAD)). Carbon tetrachloride (CCl4) was used to induce liver injuries in mice as described in previous works. A polyphenol-rich fraction from A. europaea was used at a dose of 50 mg/Kg to study its hepatoprotective effect. Next, histopathological and biochemical alterations were investigated. The HPLC analysis revealed the presence of several phenolic compounds: gallic acid, methyl gallate, rutin, ferulic acid, and resorcinol. Regarding the mice treated with a polyphenol-rich fraction from A. europaea up to 50 mg/Kg and carbon tetrachloride, no significant biochemical nor histological alterations occurred in their liver; meanwhile, serious biochemical and histopathological changes were noted for liver recovered from the mice treated with carbon tetrachloride only. In conclusion, A. europaea extract is a promising source of hepatoprotective agents against toxic liver injury.

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