scholarly journals The Morphological Features and Biology of a Relict and Endangered Woody Plant Species: Chamaedaphne calyculata (L.) Moench (Ericaceae)

Plants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 129
Author(s):  
Anna Źróbek-Sokolnik ◽  
Piotr Dynowski ◽  
Mieczysława Aldona Fenyk
Keyword(s):  
Leaf Blade ◽  
Storage Time ◽  
Seed Storage ◽  
Germination Percentage ◽  
Ex Situ ◽  
Germination Index ◽  
Western Boundary ◽  
Biometric Analysis ◽  
Chamaedaphne Calyculata ◽  
Germination Process

Chamaedaphne calyculata (L.) Moench is a rare post-glacial relic, which reaches the south-western boundary of its European range in Poland. However, little is known about the morphology and biology of this species. In this study a biometric analysis of assimilating leaves and general morphological appearance was carried out; flowering, fruiting, and seed production in a natural site were described and the ability of seed germination was determined after varied seed storage time at 0–2, 2–4, 6–8, and 21–23 °C. A quite large intrapopulation variability was found as regards the features under analysis. The most varied features included the length of the petiole, followed by the leaf blade area, while the length to the width of the leaf blade was the least varied feature. C. calyculata flowered abundantly and about 50% developed flowers turned into fruits. On average there were 56 seeds per fruit with a predominance of mature seeds. The length of the seed storage time had a significant effect on all parameters of the germination process. The temperature at which the seeds were stored, apart from the time to maximum germination parameter, had a significant effect on other parameters of the germination process. Interactions between the seeds storage time and temperature factors were observed only for germination percentage, germination index, and germination index rate parameters. The ability of seeds to germinate, non-creation of the seed bank and other results of the research provide new information necessary for planning and carrying out conservation procedures (including active conservation in ex situ conditions).

scholarly journals Experimental seed germination for ex situ conservation of Mexican Podostemaceae

2019 ◽  
Vol 97 (3) ◽  
pp. 413 ◽  
Author(s):  
Valeria Flores-Enríquez ◽  
Guillermo Castillo ◽  
Margarita Collazo-Ortega
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Light Quality ◽  
Germplasm Conservation ◽  
Seed Storage ◽  
Germination Percentage ◽  
Seed Banks ◽  
Ex Situ ◽  
Effect Of Temperature ◽  
Collection Site

<p><strong>Background</strong>: Podostemaceae are extremely susceptible to local extinction by habitat loss. Since ~70 % of the river systems in Mexico show some degree of water contamination, it is relevant to generate information about seed storage and germination behavior to design germplasm conservation strategies (<em>e.g</em>., <em>ex situ</em> seed banks) of Mexican podostemads.</p><p><strong>Hypotheses</strong>: Seed germination decreases as seed storage time increases. Further, light quality, temperature and collection site influence similarly the germination response of both species.</p><p><strong>Studied species</strong>: <em>Marathrum foeniculaceum</em> Humb. &amp; Bonpl., <em>Noveloa coulteriana</em> (Tul.)<em> </em>C. Philbrick</p><p><strong>Study site and years of study</strong>: 13 different seeds collections (1996-2013), at four locations in the rivers Horcones and Arroyo del Rincon Jalisco, México. </p><p><strong>Methods</strong>: A germination chamber experiment was performed to evaluate the effect of temperature, light quality and storage time on the germination of both species.</p><p><strong>Results</strong>: Seeds lose viability after nine years of storage. In both species, the time to reach the highest Accumulated Germination Percentage (AGP) was faster in seeds of one-two yr compared to seeds of six-seven yr. <em>N. coulteriana</em> have significant differences in Final Germination Percentage (FGP) between collection rivers. Storage time affects AGP of <em>N. </em>coulteriana more than in <em>M. foeniculaceum.</em></p><p><strong>Conclusions</strong>: Both species can form <em>ex situ</em> seed banks up to 8 yr age in paper bags storage. <em>N. coulteriana</em> is more susceptible to variation in storage conditions compared to <em>M. </em><em>foeniculaceum</em>. Collection site affect seed germination after storing.  Long-term conventional <em>ex situ</em> seed germination storage is not a viable strategy to conserve germplasm of Podostemaceae.</p>

scholarly journals Seed Germination as Assessment for “ex situ” Conservation of Diplotaxis sp

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Simona Laura LAZAR ◽  
Doru PAMFIL ◽  
Mihai Lucian LUNG
Keyword(s):  
Seed Storage ◽  
Germination Percentage ◽  
Interactive Effects ◽  
Ex Situ ◽  
Physiological Status ◽  
Before And After ◽  
The Status ◽  
Storage Behavior ◽  
Storage Methods ◽  
Selection Of

The effective seed storage methods provide a unique opportunity to preserve large amount of germplasm. Evaluation of the physiological status of the seeds before and after the storage is required for better selection of the methods and lots of seeds for conservation. To evaluate the status of the seeds, 26 accessions of different species were evaluated in two consecutive years. Four replicates of 50 seeds of each population were induced to germinate in 2010 and three replicates of 50 sees in 2011. A 2 × 2x2 factorial experiment investigated the interactive effects of accession, culture medium and year at a temperature >20OC. The germination and the Germination Velocity Index were evaluated. The results showed that there was no loss of viability before and after storage but some of the accessions had germination percentage under 50%. This happened mainly to the seeds gathered from the wild and to one commercial variety in 2010. In 2011 all accessions showed good germination with better results on agar than on filter paper.  Seeds of Diplotaxis sp possess orthodox storage behavior and therefore they are suited for ex situ conservation in seed banks.

scholarly journals EFFECT OF STORAGE TEMPERATURE AND CULTIVARS ON SEED GERMINATION OF Lilium×formolongi HORT.

2020 ◽  
Vol 8 (5) ◽  
pp. 621-627
Author(s):  
Rameshwar Rai ◽  
◽  
Jong Hwa Kim ◽  
◽  
◽  
...  
Keyword(s):  
Storage Temperature ◽  
Great Influence ◽  
Seed Storage ◽  
Germination Percentage ◽  
Temperature Treatment ◽  
Germination Index ◽  
Germination Time ◽  
Long Term Storage ◽  
Randomized Design ◽  
Mean Germination Time

An experiment was carried out to evaluate the influences of seed storage temperature and variation due to cultivars on the germination of the Lilium×formolongi hort. seeds. The seeds of four cultivars of Lilium×formolongi Hort. viz. Augusta, Raizan Herald (RH), Eorayeon 1ho, and Eorayeon 2ho was treated with four temperature ranges 5°C, -2°C, -20°C and 20°C (Room temperature, Control) up to 6 months to execute the experiment. In total 500 seeds of each cultivar including four temperature regimes and four replications for each temperature treatment and each replication represented by 30 seeds have been provided 10 days chilling treatment before to test the germination to get the uniformity in germination. The laboratory experiment was carried out in a completely randomized design (CRD) to assess the different parameters of germination viz. germination percentage(GP), mean germination time (MGT), germination index (GI), time taken to 50% germination (T50), and coefficient of the velocity of germination (CVG) at four temperature regime of four cultivars. The ANOVA analysis revealed significant variability among the four cultivars for all germination parameters in all temperature treatment levels. The results of the experiment demonstrated that the storage temperature has a great influence on germination percentage (GP), mean germination time (MGT), germination index (GI), time taken to 50% germination (T50), and coefficient of the velocity of germination (CVG) with comparisons to the normal temperature stored seeds (20°C). The significant variation among these parameters for each cultivar demonstrated the significance of varied requirements like stored temperature for the long term storage of that particular cultivar.

scholarly journals The Changes of Germination Characteristics and Enzyme Activity of Barley Seeds under Accelerated Aging

2015 ◽  
Vol 48 (2) ◽  
pp. 61-67 ◽  
Author(s):  
S A. Tabatabaei
Keyword(s):  
Enzyme Activity ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Germination Percentage ◽  
Germination Index ◽  
Accelerated Ageing ◽  
Percentage Germination ◽  
Seed Aging ◽  
Germination Characteristics ◽  
Normal Seedling

Abstract Seed aging is the main problem of seed storage. Changes of enzyme activity and reduction of seedling growth are consequence of seed deterioration. An experiment was conducted to evaluate the effects of accelerated aging on germination indexes and enzyme activity of barley seeds. Seeds were incubated in closed plastic boxes for the accelerated aging treatments at 41°C. Three accelerate aging regimes were performed by placing seeds at 41°C and relative humidity (RH) of 90-100 % for 0, 4 and 8 day periods. Our results showed that increasing aging duration resulted higher reduction in germination percentage, germination index, mean time to germination, normal seedling percentage, catalase and ascorbate peroxidase. The highest germination percentage, germination index, normal seedling percentage and enzyme activity were achieved in control conditions (0 day of aging). Under aging conditions, germination percentage, means time to germination, germination index, normal seedling percentage and enzyme activity decrease significantly. Also, our results indicated that seed aging is related to decrease of enzymes and may contribute to low germination efficiency. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed, also decrease in antioxidant enzymes is linked to an increased accelerated ageing and decreased germination characteristics. Subsequently, proposed a positive relationship between antioxidant enzyme capacity and the vigour of the seed

scholarly journals The National Tree Seed Centre Celebrates 40 Years

2007 ◽  
Vol 83 (5) ◽  
pp. 719-722
Author(s):  
J D Simpson ◽  
B S.P. Wang
Keyword(s):  
Seed Storage ◽  
Ex Situ ◽  
Future Research ◽  
Gene Conservation ◽  
Forest Land ◽  
Seed Physiology ◽  
Technology Research Group ◽  
Insect Attack ◽  
Tree Seed

The National Tree Seed Centre has been providing seed of known origin and quality for research for 40 years. Seed is also stored for long-term gene conservation purposes to provide a source of germplasm for future research and restoration. This is particularly important for species facing such threats as insect attack, disease, climate change, or conversion of forest land to non-forest uses. The Centre's inventory focuses on native tree and shrub species, striving to store samples from throughout their ranges. Over 26 000 seed samples have been sent to researchers in 65 countries, 70% of these samples being distributed within Canada. Seed research has always been a component of the Seed Centre's program. One notable accomplishment is the development of the Petawawa Germination Box. The Seed Centre participates in and contributes to activities of the Association of Official Seed Analysts, the International Seed Testing Association, and the IUFRO Seed Physiology and Technology Research Group. Key words: collection, dormancy, ex situ gene conservation, germination, research, seed, storage

scholarly journals Mid storage seed hardening: a mechanical method to maintain seed viability during long term jute seed preservation

2021 ◽  
Vol 8 (1) ◽  
pp. 79-88
Author(s):  
Md. Nasir Uddin ◽  
S. M. Mahbub Ali ◽  
Md. Abu Sadat ◽  
Md Amazed Hossain Chowdhury ◽  
Israt Jahan Mumu ◽  
...  
Keyword(s):  
Crop Production ◽  
Agricultural Sector ◽  
Seed Viability ◽  
Early Period ◽  
Chemical Properties ◽  
Seed Storage ◽  
Germination Percentage ◽  
Oil Degradation ◽  
Hardening Treatment

Seed plays an important role in agricultural sector for both production and consumption purpose. Availability of vigour seed is one of the major constraints for maximizing crop production. However, healthy seed can also lose its viability during seed storage by changing different physio-chemical properties. Influence of environmental factors and seed containers during storage leading to seed deterioration. In this research, mid storage seed hardening treatment was applied in different aged seeds of jute species (C. Capsularis & C. olitorius) with two types of storage bags. Seed hardening treatment showed the less moisture content with better germination percentage compared to the untreated species of jute seeds. Seed packing in polythene bags during both short and long term seed storages had higher viable seeds compared to the cloth packing seeds. The effect of seed hardening treatment on seed oil content and pattern of oil degradation is distinct in early period of storage. The faster rate of oil degradation, soluble protein and free amino acids was found in seeds of un-treated stored seeds in cloth bag. Contrary, very slow rate of oil degradation was observed in harden seed and stored in polythene bag which indicated better storability of harden seeds.

scholarly journals Enhancement of rice and wheat seed germination and seedling vigor by biocontrol agent, Cladosporium cladosporioides

2020 ◽  
Vol 7 (1) ◽  
pp. 9-15
Author(s):  
Md Touhidul Islam
Keyword(s):  
Seed Germination ◽  
Biocontrol Agent ◽  
Germination Percentage ◽  
Cladosporium Cladosporioides ◽  
Germination Index ◽  
Rice Seed ◽  
Seedling Vigor ◽  
Wheat Seed ◽  
Mean Values ◽  
Vigor Index

The biocontrol agent, Cladosporium cladosporioides, strain BOU1 was used to evaluate the rice and wheat plant growth by germination percentage, germination index, vigor index- I and vigor index-II in rice and wheat seed. All the variables of germination and seedling vigor of rice and wheat were increased by the conidial suspension of C. cladosporioides. The rice seed treated with C. cladosporioides had the highest germination index and vigor index-I with average mean values of 8.2 and 4.5, respectively; while the treated wheat seed showed the highest germination percentage and vigor index- II with average mean values of 80.3 and 0.05, respectively. The research is concluded that the biocontrol agent C. cladosporioides improves seed germination and seedling vigor of wheat and rice, which can finally be converted into superior yield even in adverse conditions. Res. Agric., Livest. Fish.7(1): 09-15, April 2020

scholarly journals Physiological quality of pink pepper seeds (Schinus terebinthifolia Raddi.) as a function of storage

2021 ◽  
Vol 38 (1) ◽  
pp. 144-157
Author(s):  
Thaísa De Oliveira Silveira ◽  
Madelon Rodrigues Braz ◽  
Gilmara Pires de Moura Palermo ◽  
Tiago Böer Breier
Keyword(s):  
Moisture Content ◽  
Storage Time ◽  
Storage Period ◽  
Seed Storage ◽  
Dry Weight ◽  
Native Forest ◽  
Alternative Source ◽  
Physiological Quality ◽  
And Storage

Seeds of native forest species for food and seedling production have a growing demand that impacts the need for studies involving seed storage in order to maintain good levels of germination quality. Thus, scientific analyzes that explore the physiological potential of the seeds of these species are necessary, especially for the pink pepper (Schinus terebinthifolia Raddi.), a species used as an alternative source of income for traditional communities and which has been arousing interest due to the consumption of its fruits as a condiment in the national and international market. This work aimed to assess the physiological quality of pink pepper seeds according to the storage period and seed size. The seeds came from a rural settlement, a pioneer in the extraction of pink pepper, in the state of Rio de Janeiro, Brazil. The seeds were evaluated for moisture content, germination, first count, length and dry weight of the germinated seedlings, during the period of time that they remained stored and the size of the seeds. The results allowed to conclude that: i) the germinative percentage of the stored pink pepper seeds decreased over the months; ii) the moisture content of the seeds decreased over the months evaluated iii) the different sizes of seeds did not show differences in germination and storage time and iv) values of length and dry weight of the seedlings did not differ according to the period of storage.

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