scholarly journals Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of Polygonum hydropiper L.

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 852
Author(s):  
Abdul Nasir ◽  
Mushtaq Khan ◽  
Zainab Rehman ◽  
Atif Ali Khan Khalil ◽  
Saira Farman ◽  
...  
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Polygonum Hydropiper ◽  
Isolation And Characterization ◽  
Ic50 Values

Polygonum hydropiper L. is a traditionally used medicinal plant. The present study was designed to explore the α-amylase inhibitory, antioxidant, and antimicrobial activities of Polygonum hydropiper L. Polarity-based solvent extracts (n-hexane, acetone, chloroform, methanol, ethanol, and water) of Polygonum hydropiper leaves and stem were used. Antioxidant activity was assessed by free radical scavenging assay (FRAP) and 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity methods. Quantitative phytochemical analyses suggested that the stem of Polygonum hydropiper L. contains higher levels of bioactive compounds than its leaves (p < 0.05). The results suggested that stem-derived extracts of Polygonum hydropiper L. are more active against bacterial species, including two Gram-positive and three Gram-negative strains. Moreover, our results showed that the bioactive compounds of Polygonum hydropiper L. significantly inhibit α-amylase activity. Finally, we reported the polarity-based solvent extracts of Polygonum hydropiper L. and revealed that the stem, rather than leaves, has a high antioxidant potential as measured by FRAP and DPPH assay with IC50 values of 1.38 and 1.59 mg/mL, respectively. It may also be deducted from the data that the Polygonum hydropiper L. could be a significant candidate, which should be subjected to further isolation and characterization, to be used as an antidiabetic, antimicrobial and antioxidant resource in many industries, like food, pharmaceuticals and cosmetics.

scholarly journals Composition and biological assays of the leaf essential oil of Asparagus flagellaris (Kunth) Bak

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Oluwakayode O. Odeja ◽  
Michael Gabriel Ibok ◽  
Ejike O. Okpala
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Leaf Essential Oil

Abstract Background Asparagus flagellaris leaves are ethnomedicinally used to treat syphilis, gonorrhea and other sexually transmitted diseases (STDs), with no reports on the volatile constituents. This study was aimed to quantitatively and qualitatively characterise the composition of essential oil, evaluates the free radical scavenging and antimicrobial capacity of the essential oil. Methods The essential oil was isolated by hydrodistillation method using all-glass Clevenger-type apparatus, while the identification and quantification of constituents were performed by gas chromatography-mass spectrometry (GC-MS) technique. The antioxidant activity on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) was evaluated and the oil was also tested against 10 strains of microorganisms consisting of 6 bacteria: Escherichia coli, Salmonella typhi, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis and 4 fungi: Candida albicans, Penicillium notatum, Aspergillus niger and Rhizopus spp. using broth dilution and surface plate methods, respectively. Results A pale yellow essential oil with a characteristic scent was obtained, with a yield of 0.80% (w/w). A total of 28 compounds accounting for 97.41% of the total oil contents were identified. The oil was predominated by Thymol and its derivatives, accounting for 57.48%. The most abundance (% area) constituents of the essential oil were 5-Thymyl tiglate (18.49%), Thymyl-2-methyl butyrate (17.34%), Thymol hydroquinone dimethyl ether (10.52%), Thymol methyl ether (9.42%) and 5-Propyl-1, 3-benzodioxole (4.59%). The essential oil showed a significant free radical scavenging activity compared to the standard antioxidant drugs used in this study, with % inhibition varying from 88.06 ± 0.0001 to 93.05 ± 0.0006. The leaf essential oil exhibited antimicrobial activity on all the tested organisms at 500–125 μg/mL, with an 18–10 mm inhibitory zone. Conclusion The leaf essential oil of A. flagellaris contains notable chemical compounds responsible for its antioxidant and antimicrobial activities.

scholarly journals Optimization of a Process for a Microgreen and Fruit Based Ready to Serve Beverage

2021 ◽  
pp. 41-56
Author(s):  
Anjali Sharma ◽  
Prasad Rasane ◽  
Anirban Dey ◽  
Jyoti Singh ◽  
Sawinder Kaur ◽  
...  
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Spinacia Oleracea ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Metal Chelation

A process for preparation of a microgreen and fruit based beverage was optimized using spinach (Spinacia oleracea) microgreen, pomegranate (Punica granatum), pineapple (Ananascomosus) and sugar. The blended juice in different ratios was analysed for total soluble solids, viscosity, sedimentation, acidity, metal chelation activity, free radical scavenging activity and reducing power. The optimized beverage had, 17.26 mL 100 mL-1 spinach microgreen juice, 57.07 mL 100 mL-1 pomegranate juice, 1.01 g 100 g-1 sugar and 24.66 mL 100 mL-1 pineapple juice. The product was high in nutrients, particularly protein, minerals (sodium, potassium and iron), vitamins (vitamin C), bioactive compounds (total phenols and total carotenoids) and had high antioxidant activity (metal chelation activity, free radical scavenging activity and reducing power). The antioxidants and bioactive compounds present in juice were designed to help reduce oxidative stress during inflammatory cases such as arthritis.

scholarly journals Evaluation of Free Radical Scavenging Activity in Ethanolic Extract from Promising Accessions of Curcuma aeruginosa RoxB.

Molekul ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 133 ◽  
Author(s):  
Waras Nurcholis ◽  
Nurul Khumaida ◽  
Muhamad Syukur ◽  
Maria Bintang
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Pharmaceutical Products ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Ic50 Values

This study evaluated the free radical scavenging activity in ethanolic extracts from 20 accessions of Curcuma aeruginosa. The radical scavenging activity of the extract accessions was investigated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. IC50 values for DPPH radical scavenging activity ranged from 89.81 to 505.65 µg mL-1. Based on IC50 values, twenty accessions of C. aeruginosa can be divided into three groups: strong (two accessions); moderate (seventeen accessions); and low (one accession) of DPPH scavenger. Sukoharjo (SH) and Muara Bungo (MB) showed promising accessions for antioxidant potential, thus these accessions important to selection for future breeding program in pharmaceutical products.

scholarly journals Phytochemical Screening and Free Radical Scavenging Activity of Hydroethanolic Leaf Extract of Senna sieberiana DC (Caesalpiniaceae) and its Fractions

2019 ◽  
pp. 1-6
Author(s):  
Alioune Dior Fall ◽  
Awa Ndiaye Sy ◽  
Serigne Ibra Mbacké Dieng ◽  
Abdou Sarr ◽  
Mbaye Dieng
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Ic50 Values

Objectives: The aim of this study was to identify the phytochemical groups of hydroethanolic leaf extract of Senna sieberiana DC and its fractions and to investigate their free radical scavenging activity. Methods: S. sieberiana leaves were extracted with hydroethanolic solvent. From the hydroethanolic extract 3 fractions were obtained after a liquid/liquid fractionation (dichloromethane, ethyl acetate and water). Phytochemical screening of the leaf extract and its fractions was done using standard reactions. Free radical scavenging activity was assessed using DPPH and ABTS assays.  Results: Tannins, flavonoids, anthracenic derivatives, sterols and triterpenoids were the main phytochemical constituents of the leaf extract and fractions. The hydro-ethanolic leaf extract of Senna sieberiana, its dichloromethane, ethyl acetate and aqueous fractions and ascorbic acid had respective IC50 values of 191.6±3.82 - 495.73±8.96 - 165.8±4.85 - 50.40±2.65 - 19.53±0.13 µg/ml in ABTS assay.  In DPPH assay,  the IC50 values were  44.8 ± 1.22 - 218.93±9.01 - 32.13 ± 1.8 - 26.4±0.11- 4.66±0.07 µg/ml respectively for the leaf extract, dichloromethane, ethyl acetate and aqueous fractions and ascorbic acid. Conclusion: The hydroethanolic leaf extract of S. sieberiana had shown free radical scavenging activity. The aqueous fraction was more active among plant tested samples. 

scholarly journals Free-Radical Scavenging Activity (FRSA) of Secondary Metabolite Extracted from Indonesian Eucheuma spinosum

ALCHEMY ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Nurul Inayah ◽  
Masruri Masruri
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Red Seaweed ◽  
Ic50 Values

IIndonesia is the biggest country in the production of red seaweed of Eucheuma spinosum. The red seaweed has bioactive compounds that have a potential activity such as phenolic compounds as well as carrageenan and pigments. This paper reported phytochemical analysis of E. spinosum harvested by a local farmer in Sumenep Island, East Java and free-radical scavenging activity (FRSA) derived from 2,2’-diphenyl-1-picrylhdrazyl (DPPH) for several organic solvents. For extraction, dried powder is added with 5.0 mL of various solvents following by ultra-sonication assisted extraction for 30 minutes. The extract was separated by centrifugation for phytochemical analysis and radical scavenging evaluation. The prospecting of dichloromethane, ethyl acetate, and n-hexane extracts indicated the potency for radical scavengers. Alkaloids, terpenoids, and saponins were secondary metabolites that indicated the presence in the extracts. The best IC50 value was presented by ethyl acetate extracts (384,86 ppm) with 38.78% for 50 ppm, while IC50 values of n-hexane, methanolic, dicloromethane extracts were 410.12, 677.76 and 685.08 ppm, respectively. Keywords: Eucheuma spinosum, phytochemical analysis, radical scavenging activity

Effect of heat treatment on the content of some bioactive compounds and free radical-scavenging activity in pungent and non-pungent peppers

2013 ◽  
Vol 50 (2) ◽  
pp. 519-525 ◽  
Author(s):  
José de Jesús Ornelas-Paz ◽  
Luis A. Cira-Chávez ◽  
Alfonso A. Gardea-Béjar ◽  
Juan C. Guevara-Arauza ◽  
David R. Sepúlveda ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Free Radical ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Antioxidant and Antiproliferative Activity of Selected Medicinal Plants of Lower Assam, India: An In Vitro and In Silico Study

2020 ◽  
Vol 21 (2) ◽  
pp. 267-277 ◽  
Author(s):  
Ananta Swargiary ◽  
Akalesh K. Verma ◽  
Sweta Singh ◽  
Mritunjoy K. Roy ◽  
Manita Daimari
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Tribal Communities ◽  
Isolation And Characterization

Background: The use of medicinal plants for general wellbeing and disease treatment is a common practice among tribal communities of Kokrajhar districts of Assam. However, little works have been done to study the pharmacological aspect of the plants. Objectives: The present study intends to study the antioxidant and antiproliferative properties of selected medicinal plants used by the tribal communities of Kokrajhar district of Assam since ancient times. Methods: Five traditionally important medicinal plants namely, Cassia fistula, Citrus grandis, Lindernia crustacea, Sacciolepis myosuroides, and Zingiber zerumbet were investigated for antioxidant, antiproliferative (cytotoxic) and apoptosis-inducing potential in the malignant cancer cell line. Phytochemical content such as phenolic and flavonoid content were estimated following standard protocol. The methanolic extract of plants were investigated following phosphomolybdate method (TAC), FRAP, DPPH, ABTS, and TBARS assays. Antiproliferative activities of the plants were carried out by MTT assay in DL and PBMC cells. The apoptotic study was carried out following the acridine orange and ethidium bromide staining method and fluorescent microscopic imaging. Based on the significant (P≤0.05) high apoptotic inducing potential of the plant and to further dissect the molecular mode of action including downstream biological action, major phytochemicals derived from L. crustacea were investigated for its prospective binding affinity with antiapoptotic cancer target proteins. Results: Antioxidant studies by FRAP, DPPH, ABTS, and TBARS assay revealed that all the five plants contain considerable free radical scavenging activity. C. fistula showed the strongest free radical scavenging activity while the fruit peel extract of C. grandis showed poor activity. The overall antioxidant activities of plants such as TAC, FRAP, DPPH, ABTS, and TBARS may be arranged in decreasing activity as C. fistula > Z. zerumbet > L. crustacea > S. myosuroides > C. grandis. MTT based cell proliferation study showed that all the plants extract significantly (P≤0.05) inhibited cell viability with negligible cytotoxicity (~5-12%) in normal cells. Moreover, L. crustacea showed promising antiproliferative and apoptosis-inducing ability against Dalton’s lymphoma. It is worth mentioning that the major bioactive compounds of the most potent plant extract, L. crustacea interacted with anti-apoptotic proteins (cancer target) with higher affinity and the results are compared with reference inhibitors. Conclusion: It is worth noting that these plants have the potential to consider for further scientific studies in different cell lines and animal models. Furthermore, isolation and characterization of bioactive compound(s), may promise the discovery of new and valuable drugs candidate to tackle various human diseases.

scholarly journals Preliminary investigation of Nauclea latifolia ripe fruits for antioxidant and antidiabetic activities

2019 ◽  
Vol 11 (3) ◽  
pp. 718-723
Author(s):  
Olubunmi Adenike Akinwunmi ◽  
Olalekan Christian Olatunde ◽  
Samuel Adefemi
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Preliminary Investigation ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Ic50 Values ◽  
Nauclea Latifolia

Fruits of Nauclea latifolia  (Family Rubiaceae) have been used as food and medicinal plants. The ethnomedicinal reports indicated that it can be traditionally used for the treatment of dysentery, diarrhea, diabetes, malaria etc. The aim of this work was to evaluate the antioxidant, ?–amylase and ?–glucosidase activities of methanol extract of N. latifolia fruits at varying concentrations (20-100 µg/ml) using standard methods. The results of the DPPH and nitric oxide free radical scavenging capacity showed IC50 values 92.0µg/ml and 30.0µg/ml respectively indicating a good inhibitory capacity but lesser when compared to the standard, ascorbic acid which are < 10.0µg/ml and < 20.0µg/ml respectively. The analysis shows total phenolic contents of 147.9±3.35mg/g and ferric reducing antioxidant potential of 1604.1±9.20mg/g supporting the fact that phenolics are highly potent antioxidants. Based on the results obtained from ?–amylase and ?–glucosidase inhibition, the IC50 > 100µg/ml showing a lesser inhibitory activity when compared to the standard Acarbose with IC50 values of 50.1µg/ml and 44.0µg/ml respectively. The results clearly indicate that the methanol extract of N. latifolia has a moderate free radical scavenging activity resulting from various interaction between different components of the plant. It can be concluded that the fruits may provide natural source of bioactive compounds which is beneficial to human health and can be used as basis of folkloric remedies for diabetes.

scholarly journals Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio

2020 ◽  
Vol 17 (16) ◽  
pp. 5900
Author(s):  
Joana M.C. Fernandes ◽  
Irene Fraga ◽  
Rose M.O.F. Sousa ◽  
Miguel A.M. Rodrigues ◽  
Ana Sampaio ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Free Radical ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Grape Stalks ◽  
Scanning Electron

Grape stalks, an inedible lignocellulosic residue from winemaking and agro-industrial grape juice production, can be valorized as a source of bioactive compounds and as feedstock for the saccharification and bioconversion of soluble sugars. Solid-state fermentation (SSF) by six white-rot fungi was applied as pretreatment. Fiber composition, free radical scavenging activity, four ligninolytic, and three hydrolytic enzyme activities were determined. Saccharification kinetics, yield, and productivity were evaluated and complemented with scanning electron microscopy (SEM), high performance liquid chromatography (HPLC) quantification of monosaccharides, and principal component analysis (PCA). After SSF, the biomass exhibited a drastic free radical scavenging activity decrease and the main enzymes produced were manganese-dependent peroxidase and xylanase. Scanning electron microscopy revealed the erosion of cell walls, and PCA exhibited a negative correlation between saccharification, and neutral detergent fiber and acid detergent lignin. Phlebia rufa pretreated biomass gave the highest sugars yield and productivity, representing a nearly three-fold increase compared to untreated samples. Also, monosaccharides quantification revealed that the 1:1 ratio of glucose to the sum of xylose plus galactose changes to the value of 2:1 after pretreatment. In this work, and for the first time, P. rufa proved to be an effective pretreatment of grape stalks for the saccharification and further bioconversion into value-added chemicals. In addition, lignocellulolytic enzymes were also produced through SSF.

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