scholarly journals Synthesis of New Chitosan from an Endemic Chilean Crayfish Exoskeleton (Parastacus Pugnax): Physicochemical and Biological Properties

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2304
Author(s):  
César Burgos-Díaz ◽  
Mauricio Opazo-Navarrete ◽  
José Luis Palacios ◽  
Tamara Barahona ◽  
Yohanna Mosi-Roa ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biological Properties ◽  
Bacterial Strains ◽  
Degree Of Deacetylation ◽  
Potential Applications ◽  
Chitin And Chitosan ◽  
Antimicrobial And Antioxidant Activity

Chitin is one of the most abundant natural polysaccharides in the world and it is mainly used to produce chitosan by a deacetylation process. In the present study, the extraction of chitin and chitosan from the Parastacus pugnax (P. pugnax) crayfish exoskeleton was studied for the first time. Thus, the P. pugnax crayfish exoskeleton was converted to chitosan following the steps of depigmentation, deproteinization, and deacetylation. The produced chitosan (Chitosan-CGNA) was characterized in terms of the protein content, solubility, degree of deacetylation, viscosity, molecular weight, FTIR, SEM, XRD, antimicrobial, and antioxidant activity. The results showed that the obtained chitosan had a high degree of deacetylation (91.55%) and a medium molecular weight (589.43 kDa). The antibacterial activity of the chitosan was tested against bacterial strains relevant for the food industry and the lowest minimum inhibitory concentration (MIC) values were evidenced with Salmonella tiphymurium (S. typhimurium), Staphylococcus aureus (S. aureus), Enterococcus faecalis (E. faecalis) and Listeria. Monocytogenes (L. monocytogenes). Moreover, the Chitosan-CGNA showed an effect on DPPH radical scavenging activity, and its antioxidant activity was dependent on concentration and deacetylation degree. These results suggest that P. pugnax exoskeleton could be an excellent natural source for the production of chitosan with potential applications in the health system, and to prevent infections associated with pathogens strains.

scholarly journals Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

2014 ◽  
Vol 9 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Mirjana Marčetić ◽  
Silvana Petrović ◽  
Marina Milenković ◽  
Marjan Niketić
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Volatile Fraction ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Antimicrobial And Antioxidant Activity

AbstractThe chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.

Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Anna Sip ◽  
Marta Babicka ◽  
Tomasz Rogoziński ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Content ◽  
Diffusion Method ◽  
Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

scholarly journals Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

2020 ◽  
Vol 8 (1) ◽  
pp. 75-81
Author(s):  
Bui Van Hoai ◽  
Ngo Dai Nghiep ◽  
Dao An Quang ◽  
Nguyen Thi Nam Phuong
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Free Radical ◽  
Trichoderma Viride ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Degree Of Deacetylation ◽  
Radical Scavenging Ability

Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

scholarly journals EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ISOLATED CONSTITUENTS FROM AREAL PART OF CUSCUTA REFLEXA ROXB. PLANT

2018 ◽  
Vol 11 (3) ◽  
pp. 187
Author(s):  
Sandeep Sandeep ◽  
Abhilasha Mittal ◽  
Abhilasha Mittal
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
E Coli ◽  
Cuscuta Reflexa ◽  
Isolated Compound ◽  
Antimicrobial And Antioxidant Activity ◽  
Dpph Radical Scavenging

 Objective: The objective of the study was to investigate the pharmacological evaluation of previously isolated compounds (CR-1 to CR-5) from the areal part of Cuscuta reflexa Roxb. is reported.Methods: The antimicrobial and antioxidant activity of the isolated compounds (CR-1 to CR-5) from C. reflexa was determined by the disc-diffusion method and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) model, respectively. The antimicrobial activity was performed against four strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa.Results: The results revealed that highest zone of inhibition is measured by compound CR-5 against E. coli. The antioxidant activity is evaluated for in vitro antioxidant activity using DPPH radical scavenging activity, inhibitory concentration 50% (IC50) (120.92–76.38 %), respectively. The results indicate that isolated compound CR-1 and CR-2 having IC50 76.38 and 76.94 μg/ml, respectively, showed potent antioxidant activity comparable to standard ascorbic acid (IC50 43.42 μg/ml).Conclusion: This study suggests that areal part of C. reflexa have bioactive compounds for a new antimicrobial and antioxidant drug development.

scholarly journals Chemical Composition, Antibacterial and Antioxidant Activity of the Essential Oil of Bupleurum longiradiatum

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Baojun Shi ◽  
Wei Liu ◽  
Shao-peng Wei ◽  
Wen-jun Wu
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Bupleurum Longiradiatum

The essential oil from the roots of Bupleurum longiradiatum, obtained by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS) and evaluated for antimicrobial activity and antioxidant activity. Fifty-one compounds were identified, representing 99.3% of the total oil. The major constituents were thymol (7.0%), butylidene phthalide (6.8%), 5-indolol (5.6%), heptanal (5.3%), 4-hydroxy-2-methylacetophenone (5.3%), 4,5-diethyl-octane (5.3%), bormeol (5.1%) and hexanoic acid (5.1%). The oil was tested against 4 bacteria at different concentrations using disc diffusion and 96-well dilution methods. The inhibition zones and minimum inhibitory concentration values for bacterial strains were in the range of 7.0–18.0 mm and 250 −500 μg/mL, respectively. The in vitro antioxidant activity was assessed by DPPH radical scavenging and inhibition of lipid peroxidation methods. The oil showed a potent free radical scavenging activity, as evidenced by the low IC50 value for DPPH radical (566.2μg/mL) and inhibition of lipid peroxidation (induced by FeSO4, H2O2 and CCl4) with IC50 values of 275.2 μg/mL, 296.9 μg/mL and 118.7 μg/mL, respectively.

scholarly journals Antioxidant Potential of Physicochemically Characterized Gracilaria blodgettii Sulfated Polysaccharides

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 442
Author(s):  
Bilal Muhammad Khan ◽  
Li-Xin Zheng ◽  
Wajid Khan ◽  
Aftab Ali Shah ◽  
Yang Liu ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Biological Properties ◽  
Antioxidant Potential ◽  
Random Coil ◽  
Sulfated Polysaccharides ◽  
Wide Range ◽  
Significant Difference ◽  
Potential Applications

Marine rhodophyte polysaccharides have a wide range of described biological properties with nontoxic characteristics, and show great potential in prebiotics and the functional foods industries. However, there is a virtual lack of Gracilaria blodgettii polysaccharides (GBP) profiling and their bioactivities. This study was designed while keeping in view the lack of physical and chemical characterization of GBP. This polysaccharide was also not previously tested for any bioactivities. A linear random coil conformation was observed for GBP, which was found to be a polysaccharide. A significant sulfate (w/w, 9.16%) and 3,6-anhydrogalactose (AHG, w/w, 17.97%) content was found in GBP. The significant difference in its setting (27.33 °C) and melting (64.33 °C) points makes it resistant to increasing heat. This, in turn, points to its utility in industrial scale processing and in enhancing the shelf-life of products under high temperatures. A radical scavenging activity of 19.80%, 25.42% and 8.80% was noted for GBP (3 mg/mL) in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (ABTS) and hydroxyl radical (HO) scavenging assays, respectively. Therefore, the findings suggest that Gracilaria blodgettii polysaccharides display a good antioxidant potential and may have potential applications in the functional food industry.

scholarly journals Improved Growth ofLactobacillus bulgaricusandStreptococcus thermophilusas well as Increased Antioxidant Activity by Biotransforming Litchi Pericarp Polysaccharide withAspergillus awamori

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Sen Lin ◽  
Lingrong Wen ◽  
Bao Yang ◽  
Guoxiang Jiang ◽  
John Shi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Bulgaricus ◽  
Lower Molecular Weight ◽  
Aspergillus Awamori ◽  
Protection Activity ◽  
Litchi Pericarp

This study was conducted to increase the bioactivity of litchi pericarp polysaccharides (LPPs) biotransformed byAspergillus awamori. Compared to the non-A. awamori-fermented LPP, the growth effects ofA. awamori-fermented LPP onLactobacillus bulgaricusandStreptococcus thermophiluswere four and two times higher after 3 days of fermentation, respectively. Increased 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and DNA protection activity of litchi pericarp polysaccharides were also achieved afterA. awamorifermentation. Moreover, the relative content of glucose and arabinose in LPP after fermentation decreased from 58.82% to 22.60% and from 18.82% to 10.09%, respectively, with a concomitant increase in the relative contents of galactose, rhamnose, xylose, and mannose. Furthermore, lower molecular weight polysaccharides were obtained afterA. awamorifermentation. It can be concluded thatA. awamoriwas effective in biotransforming LPP into a bioactive mixture with lower molecular weight polysaccharides and higher antioxidant activity and relative galactose content.

scholarly journals Physicochemical and microbiological characterization, and evaluation of the antimicrobial and antioxidant activity of propolis produced in two seasons and two areas of the eastern edge of the Sonoran Desert

Biotecnia ◽  
2020 ◽  
Vol 22 (3) ◽  
pp. 46-52
Author(s):  
Armida Sánchez-Escalante ◽  
Gastón Ramón Torrescano-Urrutia ◽  
Rey David Vargas-Sánchez
Keyword(s):  
Antioxidant Activity ◽  
Sonoran Desert ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Instrumental Color ◽  
Antimicrobial And Antioxidant Activity ◽  
Eastern Edge ◽  
Microbiological Properties

This research determined the effects of season and collection location on the physicochemical quality (instrumental color and proximal chemical analysis) and microbiological properties (mesophilic, psychrotrophic, and Staphylococcus aureus counts) of raw propolis, and the biological activity of propolis extracts. The total phenolic compounds (TPC) and total flavonoid content (TFC) were measured, and antioxidant activity was evaluated by the free-radical scavenging activity (FRSA) and reducing power ability (RPA). The antibacterial activity was determined against Gram-positive (S. aureus and Listeria monocytogenes) and Gram-negative bacteria (Escherichia coli and Salmonella typhimurium). The results showed that the physicochemical and microbiological properties of raw propolis complied with international quality standards. Propolis extracts collected during summer had the greatest effect against S. aureus, and the highest FRSA and RPA, likely because of also possessing the highest TPC and TFC among the extracts. In conclusion, the season and collection location affect the physicochemical properties of propolis, as well as the bioactivity of its extracts.

scholarly journals Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

2020 ◽  
Vol 15 (3) ◽  
pp. 119
Author(s):  
Ir. Yusro Nuri Fawzya, MSi ◽  
Nugrah Analiadi Putra ◽  
Arif Budi Witarto ◽  
Gintung Patantis
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Sea Cucumber ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Degree Of Hydrolysis ◽  
Dpph Method ◽  
Collagen Hydrolysates ◽  
Stichopus Horrens

Golden sea cucumber or locally known as “teripang emas” is one of Indonesia’s most popular sea cucumber and widely processed as functional food or supplement due to its bioactivities. The sea cucumber is often misidentified due to its morphological similarities with other Stichopus spp. This study aimed to identify the golden sea cucumber obtained from West Nusa Tenggara, Indonesia, by a molecular method and study the antioxidant activities of its collagen hydrolysates. The hydrolysates were produced by hydrolyzing acid collagen extract using neutrase for 30, 60, 120, 180, and 240 mins. The products were then analyzed for their degree of hydrolysis, peptide content, molecular weight distribution and radical scavenging activity by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. Results showed that hydrolysis for 180 mins was optimal in producing the highest peptide content, 12.79 ± 0.44 mg/mL, with a degree of hydrolysis (DH) of 55.2 ± 1.50%. However, the highest antioxidant activity (IC50 of 5.25 ± 0.15 mg/mL) was demonstrated after 60 mins hydrolysis with molecular weight (MW) ranged from less than 14.4 kDa to approximately 25 kDa. The hydrolysate might be categorized as a weak to moderate antioxidant. Based on the molecular identification, the golden sea cucumber had 99% similarities with Stichopus horrens and S. monotuberculatus. 

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