scholarly journals The Potential Production of the Bioactive Compound Pinene Using Whey Permeate

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 263 ◽  
Author(s):  
Derrick Risner ◽  
Maria L. Marco ◽  
Sara A. Pace ◽  
Edward S. Spang
Keyword(s):  
Scale Up ◽  
Oxygen Demand ◽  
Bioactive Compound ◽  
Waste Streams ◽  
Significant Cost ◽  
Whey Permeate ◽  
Low Concentrations ◽  
Additional Processing ◽  
Plant Metabolite ◽  
Secondary Plant Metabolite

Pinene is a secondary plant metabolite that has functional properties as a flavor additive as well as potential cognitive health benefits. Although pinene is present in low concentrations in several plants, it is possible to engineer microorganisms to produce pinene. However, feedstock cost is currently limiting the industrial scale-up of microbial pinene production. One potential solution is to leverage waste streams such as whey permeate as an alternative to expensive feedstocks. Whey permeate is a sterile-filtered dairy effluent that contains 4.5% weight/weight lactose, and it must be processed or disposed of due its high biochemical oxygen demand, often at significant cost to the producer. Approximately 180 million m3 of whey is produced annually in the U.S., and only half of this quantity receives additional processing for the recovery of lactose. Given that organisms such as recombinant Escherichia coli grow on untreated whey permeate, there is an opportunity for dairy producers to microbially produce pinene and reduce the biological oxygen demand of whey permeate via microbial lactose consumption. The process would convert a waste stream into a valuable coproduct. This review examines the current approaches for microbial pinene production, and the suitability of whey permeate as a medium for microbial pinene production.

Anaerobic/aerobic biodegradation of pentachlorophenol using GAC fluidized bed reactors: optimization of the empty bed contact time

1997 ◽  
Vol 36 (6-7) ◽  
pp. 107-115 ◽  
Author(s):  
Gregory J. Wilson ◽  
Amid P. Khodadoust ◽  
Makram T. Suidan ◽  
Richard C. Brenner
Keyword(s):  
Fluidized Bed ◽  
Oxygen Demand ◽  
Aerobic Biodegradation ◽  
Fluidized Bed Reactors ◽  
Reactor Performance ◽  
Reactor Operation ◽  
Significant Cost ◽  
Second Stage ◽  
Primary Substrate ◽  
Chlorinated Phenolic Compounds

An integrated reactor system has been developed to remediate pentachlorophenol (PCP) containing wastes using sequential anaerobic and aerobic biodegradation. Anaerobically, PCP was degraded to predominately equimolar concentrations (>99%) of monochlorophenol (MCP) in two GAC fluidized bed reactors at Empty Bed Contact Times (EBCTs) ranging from 18.6 to 1.15 hours. However, at lower EBCTs, MCP concentrations decreased to less than 10% of the influent PCP concentration suggesting mineralization. The optimal EBCT was determined to be 2.3 hours based on PCP conversion to MCPs and stable reactor operation. Decreasing the EBCT fourfold did not inhibit degradation of PCP and its intermediates, thus allowing removal of PCP at much lower detention time and resulting in a significant cost advantage. Analytical grade PCP was fed via syringe pumps into two fluidized bed reactors at influent concentrations of 100 mg/l and 200 mg/l, respectively. Acting as the primary substrate, ethanol was also fed into the reactors at concentrations of 697 and 1388 mg/l. Effluent PCP and chlorinated phenolic compounds were analyzed weekly to evaluate reactor performance. Biodegradation pathways were also identified. 3-chlorophenol (CP) was the predominant MCP and varied simultaneously with 3,5-dichlorophenol (DCP) concentrations. Likewise, 4-CP concentrations varied simultaneously with 3,4-DCP concentrations. A second stage aerobic GAC fluidized bed reactor was added after the anaerobic reactor to completely mineralize the remaining MCP and phenols. Data show no presence of phenol and MCP in the effluent or on the GAC. Overall, the chemical oxygen demand (COD) fed to the system was reduced from 75 g/d in the influent to less than 1.5 g/d in the effluent.

Post-Treatment of Effluents from Anaerobic Reactor Treating Domestic Sewage by Dissolved-Air Flotation

1999 ◽  
Vol 40 (8) ◽  
pp. 137-143 ◽  
Author(s):  
R. G. Penetra ◽  
M. A. P. Reali ◽  
E. Foresti ◽  
J. R. Campos
Keyword(s):  
Scale Up ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Domestic Sewage ◽  
Uasb Reactor ◽  
Dissolved Air Flotation ◽  
Operational Conditions ◽  
Apparent Color ◽  
Air Flotation ◽  
Dissolved Air

This paper presents the results of a study performed with a lab-scale dissolved-air flotation (LSDAF) unit fed with previously coagulated effluent from a pilot scale up-flow anerobic sludge blanket (UASB) reactor treating domestic sewage. Physical operational conditions for coagulation (rapid mix) and flocculation/flotation were maintained constant. Chemical (FeCl3) dosages ranged from 30 to 110 mg.l−1. The effect of pH was also verified in the range of 5.1 to 7.6 for each dosage. Best results were achieved for 65 mg.l−1 of FeCl3 and pH values between 5.3 and 6.1. For these conditions, the removal efficiencies obtained in the LSDAF unit were: between 87% and 91% for chemical oxygen demand (COD), between 95% and 96% for total phosphate (TP), 94% for total suspended solids (TSS), between 96% and 97% for turbidity (TU), between 90% and 93% for apparent color (AC) and more than 96% for sulfide (S). For the UASB-DAF system, global efficiencies would be around 98% for COD, 98% for TP, 98.4% for TSS, 99.3% for TU and 98% for AC. The stripped gases treatment is desirable.

scholarly journals Denitrifying Woodchip Bioreactor Leachate Tannic Acid and True Color: Lab and Field Studies

2020 ◽  
Vol 63 (6) ◽  
pp. 1747-1757
Author(s):  
Niranga M. Wickramarathne ◽  
Richard A. Cooke ◽  
Ruth Book ◽  
Laura E. Christianson
Keyword(s):  
Chemical Oxygen Demand ◽  
Tannic Acid ◽  
Acid Leaching ◽  
Oxygen Demand ◽  
The Other ◽  
List Type ◽  
N Removal ◽  
Low Concentrations ◽  
True Color ◽  
Over Time

HighlightsOak leached more tannic acid, true color, and chemical oxygen demand (COD) than ash and mixed hardwood chips.The factors became similar (tannic acid, COD) or below stream levels (true color) after flushing.Eleven site-years of field bioreactor data showed decreasing tannic acid and true color over time.Post-startup tannic acid was lower in bioreactor outflow than in area streams.True color did not appear to be a reliable indicator of leachate tannic acid at low concentrations.Abstract. Woodchips have been a preferred denitrifying bioreactor medium to date, but concerns about potential harmful effects of tannins in the leachate have precluded the use of oak chips in many installations. A study was conducted to compare the suitability of oak (genus Quercus) woodchips as a denitrifying bioreactor medium relative to other types of woodchips, both in lab leachate tests and in the context of observed bioreactor leaching in the field. Assessment measures included the content of tannic acid and other compounds in the leachate, as well as leachate color, which can often be high during startup. An 84-day leaching test using rectangular bioreactor cells filled with either oak (Quercus rubra), ash (Fraxinus spp. L.), or a generic hardwood blend showed that oak initially leached higher concentrations of tannic acid, true color, and chemical oxygen demand (COD) than the other two media. The significant differences in leached concentrations among the three wood types were eliminated after a finite leaching period. Tannic acid and true color in 11 site-years of field bioreactor outflow data generally decreased over time, except following a dry period when one of the bioreactors received no drainage inflow for more than two months. The lab and field results indicated the capability of woodchip bioreactors to flush at least these two analytes to ambient stream levels. True color did not appear to be the best parameter for estimating the tannin content of woodchip leachate due to discrepancies at low concentrations. Mass normalized tannic acid leaching ranged from 0.03 to approximately 40 mg tannic acid g-1 woodchip across the lab and field assessments. Oak initially leached more tannic acid, color, and COD than the other wood types, but the eventual similarity among the wood types after flushing with a sufficient number of pore volumes meant that any potentially negative environmental impacts would likely be limited to the startup period or possibly after dry periods. Oak initially eluted higher mean total nitrogen (TN) concentrations than the other wood types, but the treatments were not significantly different by day 3, indicating that biological N removal was not significantly inhibited, even with high concentrations of tannic acid. Keywords: Chemical oxygen demand, Oak, Tannin, Water quality, Wood leachate.

Energy-efficient wastewater treatment via the air-based, hybrid membrane biofilm reactor (hybrid MfBR)

2014 ◽  
Vol 69 (8) ◽  
pp. 1735-1741 ◽  
Author(s):  
M. Aybar ◽  
G. Pizarro ◽  
J. P. Boltz ◽  
L. Downing ◽  
R. Nerenberg
Keyword(s):  
Water Reuse ◽  
Hollow Fiber Membrane ◽  
Electrical Power ◽  
Scale Up ◽  
Oxygen Demand ◽  
Cost Savings ◽  
Hybrid Membrane ◽  
Biofilm Reactor ◽  
Membrane Biofilm Reactor ◽  
Membrane Modules

We used modeling to predict the energy and cost savings associated with the air-based, hybrid membrane-biofilm reactor (hybrid MfBR). This process is obtained by replacing fine-bubble diffusers in conventional activated sludge with air-supplying, hollow-fiber membrane modules. Evaluated processes included removal of chemical oxygen demand (COD), combined COD and total nitrogen (TN) removal, and hybrid growth (biofilm and suspended). Target concentrations of COD and TN were based on high-stringency water reuse scenarios. Results showed reductions in power requirements as high as 86%. The decrease mainly resulted from the dramatically lower air flows for the MBfR, resulting from its higher oxygen-transfer efficiencies. When the MBfR was used for COD and TN removal, savings up to US$200/1,000 m3 of treated water were predicted. Cost savings were highly sensitive to the costs of the membrane modules and electrical power. The costs were also very sensitive to membrane oxidation flux for ammonia, and the membrane life. These results suggest the hybrid MBfR may provide significant savings in energy and costs. Further research on the identified key parameters can help confirm these modeling predictions and facilitate scale-up.

scholarly journals In VitroModels for Studying Secondary Plant Metabolite Digestion and Bioaccessibility

2014 ◽  
Vol 13 (4) ◽  
pp. 413-436 ◽  
Author(s):  
M. Alminger ◽  
A.-M. Aura ◽  
T. Bohn ◽  
C. Dufour ◽  
S.N. El ◽  
...  
Keyword(s):  
Plant Metabolite ◽  
Secondary Plant Metabolite ◽  
Secondary Plant

scholarly journals Natural Treatment of Woolen Processing Industry Wastewater

2014 ◽  
Vol 10 ◽  
pp. 35-44
Author(s):  
Sajid Qurashi ◽  
O.P. Sahu
Keyword(s):  
Chemical Oxygen Demand ◽  
Industrial Waste ◽  
Oxygen Demand ◽  
Pistia Stratiotes ◽  
Wastewater Management ◽  
Waste Streams ◽  
Industrial Waste Water ◽  
Wastewater Disposal ◽  
Natural Treatment ◽  
Industry Wastewater

Wastewater treatment is becoming ever more critical due to diminishing water resources, increasing wastewater disposal costs, and stricter discharge regulations that have lowered permissible contaminant levels in waste streams. The ultimate goal of wastewater management is the protection of the environment in a manner commensurate with public health and socio-economic concerns. The aim of our study is to use natural occurring plant (Pistia stratiotes) to reduce the chemical oxygen demand and color from the industrial waste water. It was found that 120 mg/l of Chemical oxygen demand and 85 mg/l of color reduction was observed with Pistia stratiotes.

Anaerobic Treatment of Cheese Dairy Wastewater Using the SNC Bioreactor

1993 ◽  
Vol 28 (3) ◽  
pp. 597-620 ◽  
Author(s):  
Catherine N. Mulligan ◽  
Bechara F. Safi ◽  
Jacques Meunier ◽  
Jean Chebib
Keyword(s):  
Retention Time ◽  
Removal Rate ◽  
Oxygen Demand ◽  
Anaerobic Treatment ◽  
Gas Production ◽  
Cod Removal ◽  
Dairy Wastewater ◽  
Organic Load ◽  
Whey Permeate ◽  
Cod Removal Rate

Abstract The SNC multiplate reactor (1,200 L) has been developed and tested to determine chemical oxygen demand (COD) removal, nutrient requirement, and gas production from the anaerobic treatment of effluents generated at the Agropur (Notre Dame-du-Bon-Conseil, Quebec) and Nutrinor cheese dairies (Chambord, Quebec). At the Agropur plant, wastewater (3,000 mg/L COD) was treated the best at a retention time of 12 h. Using this retention time, effluents containing whey with organic loads of 10.2 to 41.6 kg COD/m3/day could be treated at a 84% COD removal rate. When the reactor was subjected to shock by increasing the organic load suddenly from 8.9 to 31 kg COD/m3/day, the total COD removal decreased to 72% and then returned to 86% after 7 days. Hydrology tests indicated that the reactor functions as a series of completely mixed stirred tanks. At Nutrinor, using a 12-h retention time and diluted whey permeate (20,000 mg/L COD), total COD removal was 86% and gas production was 12.0 m3/m3/day for a loading of 36.5 kg COD/m3/day. Nutrient supplementation was not required. For experiments performed with different proportions of wastewater (2,000 mg/L COD) to whey permeate (70,000 mg/L COD) results of 89% total and 93% soluble COD removal with a gas production of 11 m3/m3/day for a loading of 25 kg COD/m3/day were obtained. Retention times were varied from 18 to 60 h to correspond to initial CODs of 20,000 to 70,000 mg/L. In conclusion, this reactor functions in a superior manner to other published anaerobic treatment systems.

Hairy Root Culture an Alternative for Bioactive Compound Production from Medicinal Plants

2020 ◽  
Vol 22 (1) ◽  
pp. 136-149
Author(s):  
Arpita Roy
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Hairy Root ◽  
Scale Up ◽  
Root Culture ◽  
Bioactive Compound ◽  
Hairy Root Culture ◽  
Alternative Methods ◽  
Wide Range

: Medicinal plants produce a diverse group of phytocompounds like anthraquinones, alkaloids, anthocyanins, flavonoids, saponins, and terpenes which are used in pharmaceutical, perfume, cosmetics, dye and flavor industries. Commercial source of these metabolites is field-grown plants, which are generally influenced by seasonal changes. Biotechnology possesses a significant role in production of high-value secondary metabolites. By incorporating biotechnological methods, it is feasible to manage biosynthetic pathways of the plant to enhance phytocompound production that is of pharmaceutical interest. Plant cell suspension, shoot, adventitious root and hairy root culture are considered as alternative methods for important bioactive compound production. These methods are controllable, sustainable and overcome several inconveniences for large scale secondary metabolites production. At present research on hairy root culture for valuable bioactive compound production has gained a lot of attention. Agrobacterium rhizogenes is an agent which causes hairy root disease in a plant and this leads to the neoplastic growth of root which is characterized by higher growth rate and genetic stability. Various studies explore the hairy root culture for production of a wide range of bioactive compounds. Scale-up of hairy root culture using bioreactors has provided an opportunity to enhance bioactive compound production at the commercial level. The present review discusses the role of hairy root culture in the production of valuable bioactive compounds, the effect of culture parameters on bioactive compound production and bioreactor applications.

Naringenin: a potential bioactive compound and pathways of biosynthesis – a review

2021 ◽  
pp. 13-20
Keyword(s):  
Secondary Metabolites ◽  
Natural Compound ◽  
Higher Plants ◽  
Bioactive Compound ◽  
Effective Drug ◽  
Pharmacological Effects ◽  
Human Diet ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Plant Metabolite

Naringenin is a member of the flavonoid family. This natural compound represents a large proportion of secondary metabolites produced by higher plants and is a rich part of the human diet. Naringenin also has been used in the pharmaceutical and medical fields as an effective drug for anti-oxidative, anti-cancer, anti-obesity, and anti-inflammatory activities. Naringenin is also a typical plant metabolite, that has never been reported to be produced in prokaryotes. Recently, many papers reported that various members of the Streptomyces family, a genus of actinobacteria, had a novel pathway to produce naringenin. As a result, this review focuses on some clinical pharmacological effects and promising applications in the medical of naringenin, also its pathways of biosynthesis.

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