scholarly journals Comparison of Single and Combined Use of Ergothioneine, Ferulic Acid, and Glutathione as Antioxidants for the Prevention of Ultraviolet B Radiation-Induced Photoaging Damage in Human Skin Fibroblasts

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1204
Author(s):  
Gregory J. Tsay ◽  
Shin-Yi Lin ◽  
Chien-Yu Li ◽  
Jeng-Leun Mau ◽  
Shu-Yao Tsai
Keyword(s):  
Cell Viability ◽  
Ferulic Acid ◽  
Human Skin ◽  
Photochemical Reactions ◽  
Ultraviolet B ◽  
Human Skin Fibroblast ◽  
Skin Damage ◽  
Uvb Irradiation ◽  
Unirradiated Control ◽  
Combined Use

Ultraviolet B (UVB) irradiation can cause human skin damage or skin aging and wrinkle formation through photochemical reactions. Antioxidative substances may ameliorate UV damage. In this study, the anti-photoaging activity of three antioxidants—ergothioneine, ferulic acid, and glutathione—was investigated after UVB irradiation of Hs68 human skin fibroblast cells. The cells treated with these three antioxidants appeared similar to unirradiated control cells. UVB irradiation decreased cell viability by 26% compared to that of unirradiated control cells. However, the addition of either single or combined antioxidants enhanced cell viability after UVB irradiation. These three antioxidants can inhibit the production of reactive oxygen species (ROS) induced by the UVB irradiation of the Hs68 cells. Ergothioneine showed a greater inhibitory effect on matrix metalloproteinase-1 (MMP-1) performance than the other two antioxidants. IL-1 alpha was not detected in the Hs68 cells after exposure to a radiation dose of 150 mJ/cm2. Ergothioneine showed better restoration of type 1 procollagen than either ferulic acid or glutathione. Based on these results, the addition of two antioxidants was expected to restore type Ι procollagen production. In summary, these results demonstrate that the three tested antioxidants protect the skin against UVB-induced damage. The single and combined use of ergothioneine, ferulic acid, and glutathione has the potential for development as anti-photoaging materials in cosmetic applications.

scholarly journals MicroRNA-145 attenuates IL-6-induced enhancements of sensitivity to UVB irradiation by suppressing MyD88 in HaCaT cells

2018 ◽  
Vol 32 ◽  
pp. 205873841879594 ◽  
Author(s):  
Hui Dong ◽  
Wei Jiang ◽  
Hongquan Chen ◽  
Shui Jiang ◽  
Yunshu Zang ◽  
...  
Keyword(s):  
Western Blot ◽  
Cell Viability ◽  
Lupus Erythematosus ◽  
Blot Analysis ◽  
Cell Apoptosis ◽  
Western Blot Analysis ◽  
Ultraviolet B ◽  
Hacat Cells ◽  
Related Factors ◽  
Uvb Irradiation

MicroRNAs (miRNAs/miRs) play vital roles in various immune diseases including systemic lupus erythematosus (SLE). The current study aimed to assess the role of miR-145 in interleukin-6 (IL-6)-treated HaCaT cells under ultraviolet B (UVB) irradiation and further explore the potential regulatory mechanism. HaCaT cells were pretreated with IL-6 and then exposed to UVB to assess the effect of IL-6 on sensitivity of HaCaT cells to UVB irradiation. The levels of miR-145 and MyD88 were altered by transfection and the transfected efficiency was verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR)/western blot analysis. Cell viability, percentage of apoptotic cells and expression levels of apoptosis-related factors were measured by trypan blue assay, flow cytometry assay, and western blot analysis, respectively. In addition, the levels of c-Jun N-terminal kinases (JNK) and nuclear factor-κB (NF-κB) signaling pathway-related factors were assessed by western blot analysis. IL-6 treatments significantly aggravated the reduction of cell viability and promotion of cell apoptosis caused by UVB irradiation in HaCaT cells. Interestingly, miR-145 level was augmented by UVB exposure and miR-145 mimic alleviated IL-6-induced increase of sensitivity to UVB irradiation in HaCaT cells, as dramatically increased cell viability and reduced cell apoptosis. Opposite effects were observed in miR-145 inhibitor-transfected cells. Meanwhile, MyD88 was negatively regulated by miR-145 and MyD88 mediated the regulatory effect of miR-145 on IL-6- and UVB-treated cells. In addition, miR-145 mimic inhibited the JNK and NF-κB pathways by down-regulating MyD88. In conclusion, the present study demonstrated that miR-145 alleviated IL-6-induced increase of sensitivity to UVB irradiation by down-regulating MyD88 in HaCaT cells.

scholarly journals Potential Photoprotective Effect of Dietary Corn Silk Extract on Ultraviolet B-Induced Skin Damage

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2587 ◽  
Author(s):  
Kim ◽  
Cho ◽  
Kwon ◽  
Kim ◽  
Song ◽  
...  
Keyword(s):  
Target Genes ◽  
Water Extract ◽  
Ultraviolet B ◽  
Positive Staining ◽  
Mrna Levels ◽  
Skin Damage ◽  
Skin Lipid ◽  
Uvb Irradiation ◽  
Corn Silk ◽  
Anti Inflammatory

Ultraviolet B (UVB) irradiation causes adverse effects on the skin. Corn silk contains flavonoids and other bioactive compounds and antioxidants, which may prevent skin photoaging through antioxidant and anti-inflammatory effects. We aimed to investigate the potential photoprotective effects of dietary corn silk on UVB-induced skin damage in mice and the mechanisms behind these effects on human skin cells. Oral administration of corn silk water extract (CS) (2 or 4 g/kg/day) for 19 weeks decreased epidermal thickness, wrinkle formation, and positive staining for PCNA, Ki67, and 8-OHdG, and increased collagen staining in UVB-irradiated SKH-1 hairless mice compared with controls. The pro-inflammatory NF-κB target genes (IL-1β, iNOS, and COX-2) and MMP-9 expressions were lower in the CS groups, and TGF-β/Smad signaling increased. Low skin lipid peroxidation and blood DNA oxidation levels and high blood glutathione were detected. Antioxidant transcription factor Nrf2-related catalase and SOD1 proteins and glutaredoxin mRNA levels increased. The results of CS extract treatment and UVB irradiation in HaCaT cells showed the same results in Nrf2 and NF-κB target genes. An LC-MS/MS analysis showed that the CS extract contained potential antioxidants, which might have contributed to its anti-photoaging effects in tissues and cells. CS extract may reduce UVB-induced skin damage through antioxidant and anti-inflammatory mechanisms.

scholarly journals Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Di Zhang ◽  
Chengtao Lu ◽  
Zhe Yu ◽  
Xiayin Wang ◽  
Li Yan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Histopathological Examination ◽  
Ultraviolet B ◽  
Ros Generation ◽  
Antioxidant Enzyme Activities ◽  
Skin Damage ◽  
Uvb Irradiation ◽  
Uvb Exposure

Ultraviolet B (UVB) irradiation has been known to cause skin damage, which is associated with oxidative stress, DNA damage, and apoptosis. Echinacoside is a phenylethanoid glycoside isolated from Herba Cistanches, which exhibits strong antioxidant activity. In this study, we evaluate the photoprotective effect of echinacoside on UVB-induced skin damage and explore the potential molecular mechanism. BALB/c mice and HaCaT cells were treated with echinacoside before UVB exposure. Histopathological examination was used to evaluate the skin damage. Cell viability, lactate dehydrogenase (LDH) levels, antioxidant enzyme activities, reactive oxygen species (ROS) generation, DNA damage, and apoptosis were measured as well. Western blot was used to measure the expression of related proteins. The results revealed that pretreatment of echinacoside ameliorated the skin injury; attenuated oxidative stress, DNA damage, and apoptosis caused by UVB exposure; and normalized the protein levels of ATR, p53, PIAS3, hnRNP K, PARP, and XPA. To summarize, echinacoside is beneficial in the prevention of UVB-induced DNA damage and apoptosis of the skin in vivo and in vitro.

Cell Viability of Normal Human Skin Fibroblast and Fibroblasts Derived from Granulation Tissue: Effects of Nutraceuticals

2009 ◽  
Vol 12 (2) ◽  
pp. 429-434 ◽  
Author(s):  
M.H. Borawska ◽  
S.K. Czechowska ◽  
R. Markiewicz ◽  
A. Hayirli ◽  
E. Olszewska ◽  
...  
Keyword(s):  
Cell Viability ◽  
Human Skin ◽  
Granulation Tissue ◽  
Skin Fibroblast ◽  
Human Skin Fibroblast ◽  
Normal Human Skin ◽  
Tissue Effects ◽  
Normal Human ◽  
Normal Human Skin Fibroblast

scholarly journals Protective effect of total flavonoids from boxthorn leaf against UVB irradiation-induced skin injury

2021 ◽  
Vol 18 (9) ◽  
pp. 1943-1947
Author(s):  
Qing Yu ◽  
Ying Shen ◽  
Yadan Gan ◽  
Liang Zheng
Keyword(s):  
Protective Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Ultraviolet B ◽  
Control Group ◽  
Total Flavonoids ◽  
Skin Damage ◽  
Skin Injury ◽  
Uvb Irradiation ◽  
Base Group

Purpose: To investigate the protective effect of total flavonoids from boxthorn leaf against skin injury induced by UVB irradiation, and to elucidate the underlying mechanism of action. Method: Healthy female mice (n = 100) were randomly divided into four groups: normal control group, UV negative control group, cream base group, and boxthorn leaf total flavonoid (BLTF) group, with 25 mice in each group. The mice in each group were irradiated with ultraviolet B (UVB) irradiation instrument for 1.5 h daily for 3 weeks. Mice in the cream base group were smeared with cream base on their backs, while mice in BLTF group were smeared with 15 mg/g boxthorn BLTF cream. The control and negative control group mice were not treated. Changes in superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels were determined using standard methods. Results: Compared to the negative control group, the levels of SOD and GSH-Px in the control group and BLTF were significantly elevated, while MDA levels declined significantly (p < 0.05). Although higher GSH-Px and SOD levels, and lower MDA were seen in the cream base group than in negative control group, these indices were comparable for the two groups (p > 0.05). Conclusion: The total flavonoids of boxthorn leaves improve resistance to UVB-induced skin damage by regulating SOD, MDA and GSH-Px levels in the skin of mice. Thus, they exert protective effects on the skin.

Activation of HIV in Human Skin by Ultraviolet B Radiation and its Inhibition by NFκB Blocking Agents¶

2001 ◽  
Vol 74 (6) ◽  
pp. 805 ◽  
Author(s):  
Joan Breuer-McHam ◽  
Eric Simpson ◽  
Irene Dougherty ◽  
Makoto Bonkobara ◽  
Kiyoshi Ariizumi ◽  
...  
Keyword(s):  
Human Skin ◽  
Ultraviolet B ◽  
Ultraviolet B Radiation ◽  
Blocking Agents

scholarly journals UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eui Jeong Han ◽  
Seo-Young Kim ◽  
Hee-Jin Han ◽  
Hyun-Soo Kim ◽  
Kil-Nam Kim ◽  
...  
Keyword(s):  
Skin Barrier ◽  
Human Keratinocytes ◽  
Underlying Mechanism ◽  
Ultraviolet B ◽  
Sargassum Horneri ◽  
Cellular Damage ◽  
Protective Effects ◽  
Skin Damage ◽  
Antioxidant Mechanism ◽  
Induced Apoptosis

AbstractThe present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.

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