scholarly journals Characterization of Pharmaceutical Tablets Using UV Hyperspectral Imaging as a Rapid Line to Line Analysis Tool

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4436
Author(s):  
Mohammad Al Ktash ◽  
Mona Stefanakis ◽  
Barbara Boldrini ◽  
Edwin Ostertag ◽  
Marc Brecht
Keyword(s):  
Hyperspectral Imaging ◽  
Large Scale ◽  
Solid Phase ◽  
Ultra Violet ◽  
Principal Component ◽  
Ccd Camera ◽  
Conveyor Belt ◽  
Hyperspectral Data ◽  
Analysis Tool ◽  
Sample Set

A laboratory prototype for hyperspectral imaging in ultra-violet (UV) region from 225 to 400 nm was developed and used to rapidly characterize active pharmaceutical ingredients (API) in tablets. The APIs are ibuprofen (IBU), acetylsalicylic acid (ASA) and paracetamol (PAR). Two sample sets were used for a comparison purpose. Sample set one comprises tablets of 100% API and sample set two consists of commercially available painkiller tablets. Reference measurements were performed on the pure APIs in liquid solutions (transmission) and in solid phase (reflection) using a commercial UV spectrometer. The spectroscopic part of the prototype is based on a pushbroom imager that contains a spectrograph and charge-coupled device (CCD) camera. The tablets were scanned on a conveyor belt that is positioned inside a tunnel made of polytetrafluoroethylene (PTFE) in order to increase the homogeneity of illumination at the sample position. Principal component analysis (PCA) was used to differentiate the hyperspectral data of the drug samples. The first two PCs are sufficient to completely separate all samples. The rugged design of the prototype opens new possibilities for further development of this technique towards real large-scale application.

scholarly journals Drought Stress Detection in Juvenile Oilseed Rape Using Hyperspectral Imaging with a Focus on Spectra Variability

2020 ◽  
Vol 12 (20) ◽  
pp. 3462
Author(s):  
Wiktor R. Żelazny ◽  
Jan Lukáš
Keyword(s):  
Drought Stress ◽  
Hyperspectral Imaging ◽  
Oilseed Rape ◽  
Treatment Effects ◽  
Vegetation Index ◽  
Principal Component ◽  
Hyperspectral Data ◽  
Stress Detection ◽  
Mean Values ◽  
Standard Deviations

Hyperspectral imaging (HSI) has been gaining recognition as a promising proximal and remote sensing technique for crop drought stress detection. A modelling approach accounting for the treatment effects on the stress indicators’ standard deviations was applied to proximal images of oilseed rape—a crop subjected to various HSI studies, with the exception of drought. The aim of the present study was to determine the spectral responses of two cultivars, ‘Cadeli’ and ‘Viking’, representing distinctive water management strategies, to three types of watering regimes. Hyperspectral data cubes were acquired at the leaf level using a 2D frame camera. The influence of the experimental factors on the extent of leaf discolorations, vegetation index values, and principal component scores was investigated using Bayesian linear models. Clear treatment effects were obtained primarily for the vegetation indexes with respect to the watering regimes. The mean values of RGI, MTCI, RNDVI, and GI responded to the difference between the well-watered and water-deprived plants. The RGI index excelled among them in terms of effect strengths, which amounted to −0.96[−2.21,0.21] and −0.71[−1.97,0.49] units for each cultivar. A consistent increase in the multiple index standard deviations, especially RGI, PSRI, TCARI, and TCARI/OSAVI, was associated with worsening of the hydric regime. These increases were captured not only for the dry treatment but also for the plants subjected to regeneration after a drought episode, particularly by PSRI (a multiplicative effect of 0.33[0.16,0.68] for ‘Cadeli’). This result suggests a higher sensitivity of the vegetation index variability measures relative to the means in the context of the oilseed rape drought stress diagnosis and justifies the application of HSI to capture these effects. RGI is an index deserving additional scrutiny in future studies, as both its mean and standard deviation were affected by the watering regimes.

A Hyperspectral Imaging System for Identification of Faecal and Ingesta Contamination on Poultry Carcasses

2003 ◽  
Vol 11 (4) ◽  
pp. 269-281 ◽  
Author(s):  
Kurt C. Lawrence ◽  
William R. Windham ◽  
Bosoon Park ◽  
R. Jeff Buhr
Keyword(s):  
Real Time ◽  
Hyperspectral Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Principal Component ◽  
Ccd Camera ◽  
Sample Population ◽  
Multivariate System ◽  
Ratio Image ◽  
On Line

A method and system for detecting faecal and ingesta contaminants on poultry carcasses were demonstrated. A visible/near infrared monochromator, which measured reflectance and principal component analysis were first used to identify key wavelengths from faecal and uncontaminated skin samples. Measurements at 434, 517, 565 and 628 nm were identified and used for evaluation with a hyperspectral imaging system. The hyperspectral imaging system, which was a line-scan (pushbroom) imaging system, consisted of a hyperspectral camera, fibre-optic line lights, a computer and frame grabber. The hyperspectral imaging camera consisted of a high-resolution charge coupled device (CCD) camera, a prism-grating-prism spectrograph, focusing lens, associated optical hardware and a motorised controller. The imaging system operated from about 400 to 900 nm. The hyperspectral imaging system was calibrated for wavelength, distance and percent reflectance and analysis of calibrated images at the key wavelengths indicated that single-wavelength images were inadequate for detecting contaminants. However, a ratio of images at two of the key wavelengths was able to identify faecal and ingesta contaminants. Specifically, the ratio of the 565-nm image divided by the 517-nm image produced good results. The ratio image was then further processed by masking the background and either enhancing the image contrast with a non-linear histogram stretch, or applying a faecal threshold. The results indicated that, for the limited sample population, more than 96% of the contaminants were detected. Thus, the hyperspectral imaging system was able to detect contaminants and showed feasibility, but was too slow for real-time on-line processing. Therefore, a multivariate system operating at 565 and 517 nm, which should be capable of operating at real-time on-line processing speed, should be used. Further research with such a system needs to be conducted.

scholarly journals Ultraviolet-visible/near infrared spectroscopy and hyperspectral imaging to study the different types of raw cotton

2020 ◽  
Author(s):  
Mohammad Al Ktash ◽  
Otto Hauler ◽  
Edwin Ostertag ◽  
Marc Brecht
Keyword(s):  
Hyperspectral Imaging ◽  
Large Scale ◽  
Near Infrared ◽  
Dominant Role ◽  
Principal Component ◽  
Industrial Applications ◽  
Acquisition Time ◽  
Hydroxyl Groups ◽  
Short Acquisition Time ◽  
Different Types

Different types of raw cotton were investigated by a commercial ultraviolet-visible/near infrared (UV-Vis/NIR) spectrometer (210–2200 nm) as well as on a home-built setup for NIR hyperspectral imaging (NIR-HSI) in the range 1100–2200 nm. UV-Vis/NIR reflection spectroscopy reveals the dominant role proteins, hydrocarbons and hydroxyl groups play in the structure of cotton. NIR-HSI shows a similar result. Experimentally obtained data in combination with principal component analysis (PCA) provides a general differentiation of different cotton types. For UV-Vis/NIR spectroscopy, the first two principal components (PC) represent 82 % and 78 % of the total data variance for the UV-Vis and NIR regions, respectively. Whereas, for NIR-HSI, due to the large amount of data acquired, two methodologies for data processing were applied in low and high lateral resolution. In the first method, the average of the spectra from one sample was calculated and in the second method the spectra of each pixel were used. Both methods are able to explain ≥90 % of total variance by the first two PCs. The results show that it is possible to distinguish between different cotton types based on a few selected wavelength ranges. The combination of HSI and multivariate data analysis has a strong potential in industrial applications due to its short acquisition time and low-cost development. This study opens a novel possibility for a further development of this technique towards real large-scale processes.

Detection of Nitrogen Content Changes of Rape Leaf Using Hyperspectral Imaging

2011 ◽  
Vol 204-210 ◽  
pp. 131-134 ◽  
Author(s):  
Wei Zou ◽  
Hui Fang ◽  
Yi Dan Bao ◽  
Yong He
Keyword(s):  
Neural Network ◽  
Nitrogen Content ◽  
Hyperspectral Imaging ◽  
Imaging System ◽  
Principal Component ◽  
Hyperspectral Data ◽  
Spad Value ◽  
Good Classification ◽  
Optimal Wavelength ◽  
Artificial Neural Network Ann

Hyperspectral imaging (400-1000nm) and artificial neural network (ANN) techniques were investigated for the detection of nitrogen content changes of rape leaf. Measuring SPAD value of rape leaf by using SPAD (Soil and Plant Analyzer Development).A hyperspectral imaging system was established to acquire hyperspectral data. Principal component analysis(PCA) was used to obtain principal component images, as well as to select the optimal wavelength(s). ANN was applied to establish the model between the spectral reflection values and SPAD values. The prediction results were obtained for the nitrogen content of rape leaf with the correlation of prediction of R=0.9237. The results show that the hyperspectral imaging has good classification on different nitrogen content of rape leaf.

scholarly journals Rapid Classification of Wheat Grain Varieties Using Hyperspectral Imaging and Chemometrics

2019 ◽  
Vol 9 (19) ◽  
pp. 4119 ◽  
Author(s):  
Yidan Bao ◽  
Chunxiao Mi ◽  
Na Wu ◽  
Fei Liu ◽  
Yong He
Keyword(s):  
Hyperspectral Imaging ◽  
Large Scale ◽  
Scatter Correction ◽  
Principal Component ◽  
Support Vector ◽  
Wheat Grain ◽  
Successive Projections Algorithm ◽  
Accurate Identification ◽  
Wheat Varieties

The classification of wheat grain varieties is of great value because its high purity is the yield and quality guarantee. In this study, hyperspectral imaging combined with the chemometric methods was applied to explore and implement the varieties classification of wheat seeds. The hyperspectral images of all the samples covering 874–1734 nm bands were collected. Exploratory analysis was first carried out while using principal component analysis (PCA) and linear discrimination analysis (LDA). Spectral preprocessing methods including standard normal variate (SNV), multiplicative scatter correction (MSC), and wavelet transform (WT) were introduced, and their effects on discriminant models were studied to eliminate the interference of instrumental and environmental factors. PCA loading, successive projections algorithm (SPA), and random frog (RF) were applied to extract feature wavelengths for redundancy elimination owing to the possibility of existing redundant spectral information. Classification models were developed based on full wavelengths and feature wavelengths using LDA, support vector machine (SVM), and extreme learning machine (ELM). This optimal model was finally utilized to generate visualization map to observe the classification performance intuitively. When comparing with other models, ELM based on full wavelengths achieved the best accuracy up to 91.3%. The overall results suggested that hyperspectral imaging was a potential tool for the rapid and accurate identification of wheat varieties, which could be conducted in large-scale seeds classification and quality detection in modern seed industry.

scholarly journals Segment-Based Clustering of Hyperspectral Images Using Tree-Based Data Partitioning Structures

Algorithms ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 330
Author(s):  
Mohamed Ismail ◽  
Milica Orlandić
Keyword(s):  
Large Scale ◽  
Clustering Algorithm ◽  
Hyperspectral Image ◽  
Principal Component ◽  
Data Partitioning ◽  
Hyperspectral Data ◽  
Compact Representation ◽  
Parametric Modelling ◽  
Ground Truth Data ◽  
Binary Partition

Hyperspectral image classification has been increasingly used in the field of remote sensing. In this study, a new clustering framework for large-scale hyperspectral image (HSI) classification is proposed. The proposed four-step classification scheme explores how to effectively use the global spectral information and local spatial structure of hyperspectral data for HSI classification. Initially, multidimensional Watershed is used for pre-segmentation. Region-based hierarchical hyperspectral image segmentation is based on the construction of Binary partition trees (BPT). Each segmented region is modeled while using first-order parametric modelling, which is then followed by a region merging stage using HSI regional spectral properties in order to obtain a BPT representation. The tree is then pruned to obtain a more compact representation. In addition, principal component analysis (PCA) is utilized for HSI feature extraction, so that the extracted features are further incorporated into the BPT. Finally, an efficient variant of k-means clustering algorithm, called filtering algorithm, is deployed on the created BPT structure, producing the final cluster map. The proposed method is tested over eight publicly available hyperspectral scenes with ground truth data and it is further compared with other clustering frameworks. The extensive experimental analysis demonstrates the efficacy of the proposed method.

scholarly journals Identification of Cold Spots Using Non-Destructive Hyperspectral Imaging Technology in Model Food Processed by Coaxially Induced Microwave Pasteurization and Sterilization

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 837 ◽  
Author(s):  
Aswathi Soni ◽  
Mahmoud Al-Sarayreh ◽  
Marlon M. Reis ◽  
Jeremy Smith ◽  
Kris Tong ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Control Sample ◽  
Principal Component ◽  
Hyperspectral Data ◽  
Cold Spot ◽  
Maillard Reaction Products ◽  
Reaction Products ◽  
Analysis Model ◽  
Model Food ◽  
Microwave Pasteurization

The model food in this study known as mashed potato consisted of ribose (1.0%) and lysine (0.5%) to induce browning via Maillard reaction products. Mashed potato was processed by Coaxially Induced Microwave Pasteurization and Sterilization (CiMPAS) regime to generate an F0 of 6–8 min and analysis of the post-processed food was done in two ways, which included by measuring the color changes and using hyperspectral data acquisition. For visualizing the spectra of each tray in comparison with the control sample (raw mashed-potato), the mean spectrum (i.e., mean of region of interest) of each tray, as well as the control sample, was extracted and then fed to the fitted principal component analysis model and the results coincided with those post hoc analysis of the average reflectance values. Despite the presence of a visual difference in browning, the Lightness (L) values were not significantly (p < 0.05) different to detect a cold spot among a range of 12 processed samples. At the same time, hyperspectral imaging could identify the colder trays among the 12 samples from one batch of microwave sterilization.

scholarly journals Spatial Distortion Assessments of a Low-Cost Laboratory and Field Hyperspectral Imaging System

Sensors ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 4267
Author(s):  
Andrija Krtalić ◽  
Vanja Miljković ◽  
Dubravko Gajski ◽  
Ivan Racetin
Keyword(s):  
Hyperspectral Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Low Cost ◽  
Ccd Camera ◽  
Hyperspectral Data ◽  
Modulation Transfer ◽  
Spatial Distortion ◽  
Visible Part ◽  
Spatial Calibration

This article describes the adaptation of an existing aerial hyperspectral imaging system in a low-cost setup for collecting hyperspectral data in laboratory and field environment and spatial distortion assessments. The imaging spectrometer system consists of an ImSpector V9 hyperspectral pushbroom scanner, PixelFly high performance digital CCD camera, and a subsystem for navigation, position determination and orientation of the system in space, a sensor bracket and control system. The main objective of the paper is to present the system, with all its limitations, and a spatial calibration method. The results of spatial calibration and calculation of modulation transfer function (MTF) are reported along with examples of images collected and potential uses in agronomy. The distortion value rises drastically at the edges of the image in the near-infrared segment, while the results of MTF calculation showed that the image sharpness was equal for the bands from the visible part of the spectrum, and approached Nyquist’s theory of digitalization. In the near-infrared part of the spectrum, the MTF values showed a less sharp decrease in comparison with the visible part. Preliminary image acquisition indicates that this hyperspectral system has potential in agronomic applications.

scholarly journals Functional Hyperspectral Imaging by High-Related Vegetation Indices to Track the Wide-Spectrum Trichoderma Biocontrol Activity Against Soil-Borne Diseases of Baby-Leaf Vegetables

2021 ◽  
Vol 12 ◽  
Author(s):  
Gelsomina Manganiello ◽  
Nicola Nicastro ◽  
Michele Caputo ◽  
Massimo Zaccardelli ◽  
Teodoro Cardi ◽  
...  
Keyword(s):  
Biological Control ◽  
Hyperspectral Imaging ◽  
Large Scale ◽  
Plant Pathogens ◽  
Disease Incidence ◽  
Vegetation Indices ◽  
Screening Method ◽  
Hyperspectral Data ◽  
Screening Methods

Research has been increasingly focusing on the selection of novel and effective biological control agents (BCAs) against soil-borne plant pathogens. The large-scale application of BCAs requires fast and robust screening methods for the evaluation of the efficacy of high numbers of candidates. In this context, the digital technologies can be applied not only for early disease detection but also for rapid performance analyses of BCAs. The present study investigates the ability of different Trichoderma spp. to contain the development of main baby-leaf vegetable pathogens and applies functional plant imaging to select the best performing antagonists against multiple pathosystems. Specifically, sixteen different Trichoderma spp. strains were characterized both in vivo and in vitro for their ability to contain R. solani, S. sclerotiorum and S. rolfsii development. All Trichoderma spp. showed, in vitro significant radial growth inhibition of the target phytopathogens. Furthermore, biocontrol trials were performed on wild rocket, green and red baby lettuces infected, respectively, with R. solani, S. sclerotiorum and S. rolfsii. The plant status was monitored by using hyperspectral imaging. Two strains, Tl35 and Ta56, belonging to T. longibrachiatum and T. atroviride species, significantly reduced disease incidence and severity (DI and DSI) in the three pathosystems. Vegetation indices, calculated on the hyperspectral data extracted from the images of plant-Trichoderma-pathogen interaction, proved to be suitable to refer about the plant health status. Four of them (OSAVI, SAVI, TSAVI and TVI) were found informative for all the pathosystems analyzed, resulting closely correlated to DSI according to significant changes in the spectral signatures among health, infected and bio-protected plants. Findings clearly indicate the possibility to promote sustainable disease management of crops by applying digital plant imaging as large-scale screening method of BCAs' effectiveness and precision biological control support.

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