Diamine Oxidase-Conjugated Multiwalled Carbon Nanotubes to Facilitate Electrode Surface Homogeneity

Carbon nanomaterials have gained significant interest over recent years in the field of electrochemistry, and they may be limited in their use due to issues with their difficulty in dispersion. Enzymes are prime components for detecting biological molecules and enabling electrochemical interactions, but they may also enhance multiwalled carbon nanotube (MWCNT) dispersion. This study evaluated a MWCNT and diamine oxidase enzyme (DAO)-functionalised screen-printed electrode (SPE) to demonstrate improved methods of MWCNT functionalisation and dispersion. MWCNT morphology and dispersion was determined using UV-Vis spectroscopy (UV-Vis) and scanning electron microscopy (SEM). Carboxyl groups were introduced onto the MWCNT surfaces using acid etching. MWCNT functionalisation was carried out using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS), followed by DAO conjugation and glutaraldehyde (GA) crosslinking. Modified C-MWNCT/EDC-NHS/DAO/GA was drop cast onto SPEs. Modified and unmodified electrodes after MWCNT functionalisation were characterised using optical profilometry (roughness), water contact angle measurements (wettability), Raman spectroscopy and energy dispersive X-ray spectroscopy (EDX) (vibrational modes and elemental composition, respectively). The results demonstrated that the addition of the DAO improved MWCNT homogenous dispersion and the solution demonstrated enhanced stability which remained over two days. Drop casting of C-MWCNT/EDC-NHS/DAO/GA onto carbon screen-printed electrodes increased the surface roughness and wettability. UV-Vis, SEM, Raman and EDX analysis determined the presence of carboxylated MWCNT variants from their non-carboxylated counterparts. Electrochemical analysis demonstrated an efficient electron transfer rate process and a diffusion-controlled redox process. The modification of such electrodes may be utilised for the development of biosensors which could be utilised to support a range of healthcare related fields.

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Electroanalytical and electrocatalytical characteristics of cytochrome P450 3A4 using electrodes modified with nanocomposite carbon nanomaterials

Biomeditsinskaya Khimiya ◽

10.18097/pbmc20206601064 ◽

2020 ◽

Vol 66 (1) ◽

pp. 64-70 ◽

Cited By ~ 1

Author(s):

A.V. Kuzikov ◽

T.V. Bulko ◽

P.I. Koroleva ◽

R.A. Masamrekh ◽

S.S. Babkina ◽

...

Keyword(s):

Carbon Nanotubes ◽

Cytochrome P450 ◽

Carbon Nanomaterials ◽

Electrochemical Analysis ◽

Heme Iron ◽

Cytochrome P450 3A4 ◽

Graphite Electrodes ◽

Protein Film ◽

Protein Film Voltammetry ◽

Screen Printed

The electroanalytical characteristics of recombinant cytochrome P450 3A4 (P450 3A4) immobilized on the surface of screen-printed graphite electrodes modified with multi-walled carbon nanotubes have been studied. The role and the influence of graphite working electrode modification with carbon nanotubes on electroanalytical characteristics of cytochrome P450 3A4 have been demonstrated. The conditions for the immobilization of cytochrome P450 3A4 on the obtained screen-printed graphite electrodes modified with carbon multi-walled nanotubes have been optimized. The electrochemical parameters of the oxidation and reduction of the heme iron of the enzyme have been estimated. The midpoint potential E0′ was -0.35±0.01 V vs Ag/AgCl; the calculated heterogeneous electron transfer rate constant ks, was 0.57±0.04 s-1; the amount of electroactive cytochrome P450 3A4 on the electrode Г0, was determined as (2.6±0.6)⋅10-10 mol/cm2. The functioning mechanism of P450 3A4-based electrochemical sensor followed the “protein film voltammetry”. In order to develop electrochemical analysis of drugs being substrates of that hemoprotein and respective medical biosensors the voltammetric study of catalytic activity of immobilized cytochrome P450 3A4 was carried out. Electrocatalytic properties of cytochrome P450 3A4, immobilized on modified screen-printed graphite electrodes, has been investigated using erythromycin (macrolide antibiotics). It has been shown that the modification of electrodes plays a decisive role for the study of the properties of cytochromes P450 in electrochemical investigations. Smart electrodes can serve as sensors for analytical purposes, as well as electrocatalysts for the study of biotransformation processes and metabolic processes. Electrodes modified with carbon nanomaterials are applicable for analytical purposes in the registration of hemoproteins. Electrodes modified with synthetic membrane-like compounds (e.g. didodecyldimethylammonium bromide) are effective in enzyme-dependent electrocatalysis.

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Scalable fabrication of carbon materials based silicon rubber for highly stretchable e-textile sensor

Nanotechnology Reviews ◽

10.1515/ntrev-2020-0088 ◽

2020 ◽

Vol 9 (1) ◽

pp. 1183-1191

Author(s):

Xinlin Li ◽

Rixuan Wang ◽

Leilei Wang ◽

Aizhen Li ◽

Xiaowu Tang ◽

...

Keyword(s):

Carbon Nanomaterials ◽

Electrical Performance ◽

Gauge Factor ◽

Multiwalled Carbon ◽

Silicon Rubber ◽

Smart Clothing ◽

Mechanical And Electrical Properties ◽

Highly Stretchable ◽

Textile Sensor ◽

High Level

AbstractDevelopment of stretchable wearable devices requires essential materials with high level of mechanical and electrical properties as well as scalability. Recently, silicone rubber-based elastic polymers with incorporated conductive fillers (metal particles, carbon nanomaterials, etc.) have been shown to the most promising materials for enabling both high electrical performance and stretchability, but the technology to make materials in scalable fabrication is still lacking. Here, we propose a facile method for fabricating a wearable device by directly coating essential electrical material on fabrics. The optimized material is implemented by the noncovalent association of multiwalled carbon nanotube (MWCNT), carbon black (CB), and silicon rubber (SR). The e-textile sensor has the highest gauge factor (GF) up to 34.38 when subjected to 40% strain for 5,000 cycles, without any degradation. In particular, the fabric sensor is fully operational even after being immersed in water for 10 days or stirred at room temperature for 8 hours. Our study provides a general platform for incorporating other stretchable elastic materials, enabling the future development of the smart clothing manufacturing.

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Silver mirror reaction metallized chromatography paper for supercapacitor application

Flexible and Printed Electronics ◽

10.1088/2058-8585/ac3a13 ◽

2021 ◽

Author(s):

I-Hsuan Chen ◽

Jung-Hsien Chang ◽

Ren-Jie Xie ◽

Chia-Hui Tseng ◽

Sheng-Rong Hsieh ◽

...

Keyword(s):

Flexible Electronics ◽

Photoelectron Spectroscopy ◽

Cost Effective ◽

Water Contact ◽

X Ray ◽

Supercapacitor Application ◽

Resource Limited ◽

Vis Spectroscopy ◽

Silver Mirror ◽

Silver Mirror Reaction

Abstract In this study, the easy-to-operate silver mirror reaction (SMR) was used for metallizing chromatography paper. The SMR-metallized paper was characterized by water contact angle measurements, a surface profiler, X-ray photoelectron spectroscopy, UV-vis spectroscopy, X-ray diffraction, and electrical resistance measurement. The characterization results show that Ag was successfully synthesized on cellulose fibers and was electrically conductive after cyclic bending. Moreover, this SMR-metallized paper was used as electrodes for fabricating a supercapacitor. This SMR-metallized paper could be used for realizing cost-effective flexible electronics applied in on-site biochemical sensing in resource-limited settings.

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Electrochemical Analysis of Acrylamide Using Screen-Printed Carboxylated Single-Walled Carbon Nanotube Electrodes

Electroanalysis ◽

10.1002/elan.201300636 ◽

2014 ◽

Vol 26 (5) ◽

pp. 1039-1044 ◽

Cited By ~ 6

Author(s):

Fanny Jaqueline González-Fuentes ◽

J. Manríquez ◽

Luis A. Godínez ◽

Alberto Escarpa ◽

Sandra Mendoza

Keyword(s):

Carbon Nanotube ◽

Electrochemical Analysis ◽

Single Walled Carbon Nanotube ◽

Single Walled Carbon ◽

Screen Printed

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Preparation of TiO2–SiO2 Hybrid Nanosols Coated Flame-Retardant Polyester Fabric Possessing Dual Contradictory Characteristics of Superhydrophobicity and Self Cleaning Ability

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2020.17166 ◽

2020 ◽

Vol 20 (3) ◽

pp. 1780-1789 ◽

Cited By ~ 5

Author(s):

Priyanka Katiyar ◽

Shraddha Mishra ◽

Anurag Srivastava ◽

N. Eswara Prasad

Keyword(s):

Electron Microscopy ◽

Contact Angle ◽

Flame Retardant ◽

Protective Clothing ◽

Sol Gel ◽

Contact Angle Measurement ◽

Angle Measurement ◽

Water Contact ◽

Vis Spectroscopy ◽

Self Cleaning

TiO2, SiO2 and their hybrid nanocoatings are prepared on inherent flame retardant textile substrates from titanium(IV)iso-proproxide (TTIP) and tetraethoxysilane (TEOS) precursors using a sol–gel process followed by hydrothermal treatment. The coated samples are further functionalized by hexadecyltrimethoxysilane (HDTMS) to impart superhydrophobicity. Sample characterization of the nanosols, nanoparticles and coated samples are investigated using, X-ray diffractometer, transmission electron microscopy, scanning electron microscopy, UV-Vis spectroscopy, contact angle measurement. Stain degradation test under mild UV irradiation shows almost 54% degradation of coffee stain within 4 hours measured by Spectrophotometer. UV-Vis Absorption Spectroscopy demonstrates complete degradation of methyl orange colorant within 3 hours. Hybrid nanosol coated and HDTMS modified inherent flame retardant polyester surfaces show apparent water contact angle as ~145°, which is much closer to proximity of superhydrophobic surfaces. Thus, the novelty of present work is, by using sol–gel technique, a bi-functional textile surface has been developed which qualifies the very specific requirements of protective clothing like self-cleaning property (imparted by TiO2 nanoparticles) and superhydrophobicity (imparted by SiO2 nanoparticles and further surface modification by HDTMS), which are entirely contradictory in nature, in a single fabric itself. Thus developed textile surfaces also possess the other attributes of protective clothing like flame retardancy and air permeability.

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Effect of Modified Silica Materials on Polyvinyl Chloride (PVC) Substrates to Obtain Transparent and Hydrophobic Hybrid Coatings

Applied Sciences ◽

10.3390/app112211044 ◽

2021 ◽

Vol 11 (22) ◽

pp. 11044

Author(s):

Violeta Purcar ◽

Valentin Rădițoiu ◽

Alina Rădițoiu ◽

Florentina Monica Raduly ◽

Georgiana Cornelia Ispas ◽

...

Keyword(s):

Polyvinyl Chloride ◽

Sol Gel ◽

Contact Angles ◽

Hybrid Coatings ◽

Water Contact ◽

Silica Coatings ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Silica Materials ◽

Window Blinds

In this research, we report a simple and inexpensive way to prepare transparent and hydrophobic hybrid coatings through deposition of different silica materials on polyvinyl chloride (PVC) substrates. The silica materials were prepared using an acid-catalyzed sol–gel method at room temperature (25 ± 2 °C), using alkoxysilanes: tetraethoxysilane (TEOS), as the silica source, and ethoxydimethylvinylsilane (DMVES), triethoxyoctylsilane (OTES), and trimethoxyhexadecylsilane (HDTMES), as modifier agents. The obtained materials were characterized (either as powders or as thin films) by Fourier-transform infrared spectroscopy (FTIR), UV/Vis spectroscopy, transmission electron microscopy (TEM), thermogravimetric analysis (TGA), atomic force microscopy (AFM), spectroscopic ellipsometry (SE), and water contact-angle measurements. UV/Vis spectra showed that the PVC substrate coated with the silica material containing TEOS/DMVES/OTES had a transmittance of about 90% in the wavelength range of 650–780 nm. The water contact angles increased from 83° for uncoated PVC substrate to ~94° for PVC substrates coated with the sol–gel silica materials. These PVC films with hybrid silica coatings can be used as the materials for outdoor applications, such as energy-generating solar panel window blinds or PVC clear Windmaster outdoor blinds.

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Lyophilised legume sprouts as a functional ingredient for diamine oxidase enzyme supplementation in histamine intolerance

LWT ◽

10.1016/j.lwt.2020.109201 ◽

2020 ◽

Vol 125 ◽

pp. 109201 ◽

Cited By ~ 1

Author(s):

Oriol Comas-Basté ◽

M. Luz Latorre-Moratalla ◽

Judit Rabell-González ◽

M. Teresa Veciana-Nogués ◽

M. Carmen Vidal-Carou

Keyword(s):

Diamine Oxidase ◽

Functional Ingredient ◽

Histamine Intolerance ◽

Oxidase Enzyme ◽

Enzyme Supplementation

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Biotransformation of Pristine and Oxidized Carbon Nanotubes by the White Rot Fungus Phanerochaete chrysosporium

Nanomaterials ◽

10.3390/nano9091340 ◽

2019 ◽

Vol 9 (9) ◽

pp. 1340 ◽

Cited By ~ 3

Author(s):

Qiang Ma ◽

Ailimire Yilihamu ◽

Zhu Ming ◽

Shengnan Yang ◽

Mengyao Shi ◽

...

Keyword(s):

Carbon Nanotubes ◽

Multiwalled Carbon Nanotubes ◽

Phanerochaete Chrysosporium ◽

Environmental Remediation ◽

Carbon Nanomaterials ◽

Carbon Skeleton ◽

White Rot ◽

White Rot Fungus ◽

Multiwalled Carbon ◽

Complete Degradation

Carbon nanomaterials are widely studied and applied nowadays, with annual production increasing. After entering the environment, the complete degradation of these carbon nanomaterials by microorganisms is proposed as an effective approach for detoxification and remediation. In this study, we evaluated the degradation of pristine multiwalled carbon nanotubes (p-MWCNTs) and oxidized multiwalled carbon nanotubes (o-MWCNTs) by the white rot fungus Phanerochaete chrysosporium, which is a powerful decomposer in the carbon cycle and environmental remediation. Both p-MWCNTs and o-MWCNTs were partially oxidized by P. chrysosporium as indicated by the addition of oxygen atoms to the carbon skeleton in the forms of C=O and O–H bonds. The fungal oxidation led to the shortening of MWCNTs, where precipitated o-MWCNTs showed more short tubes. During the transformation, the defects on the tubes became detached from the carbon skeleton, resulting in decreases of the ID/IG (intensity of D-band/ intensity of G-band) values in Raman spectra. The transformation mechanism was attributed to the enzymatic degradation by laccase and manganese peroxidase excreted by P. chrysosporium. The results collectively indicated that MWCNTs could be transformed by P. chrysosporium, but complete degradation could not be achieved in a short time period. The implications on the environmental risks of carbon nanomaterials are discussed.

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DIFFERENCES IN CYTOCOMPATIBILITY BETWEEN MWCNTs AND CARBOXYLIC FUNCTIONALIZED MWCNTs

Functional Materials Letters ◽

10.1142/s1793604712500531 ◽

2013 ◽

Vol 06 (01) ◽

pp. 1250053 ◽

Cited By ~ 4

Author(s):

YITENG ZHANG ◽

DEJUN LI ◽

MENGLI ZHAO ◽

MEIXIAN GUO ◽

XIANGYUN DENG ◽

...

Keyword(s):

Contact Angle ◽

Umbilical Vein ◽

Mouse Fibroblast ◽

Carboxyl Groups ◽

Fibroblast Cells ◽

Functional Surface ◽

Multiwalled Carbon ◽

Water Contact ◽

Umbilical Vein Endothelial Cells ◽

Functionalized Mwcnts

Influence of carboxylic functionalization on the cytocompatibility of multiwalled carbon nanotubes (MWCNTs) was investigated in this work. Water contact angle assay showed that the surface of MWCNTs-containing carboxyl (MWCNTs-COOH) became much more hydrophilic compared with pure MWCNTs. In cell-adhesion assays, two cell lines, mouse fibroblast cells (L929) and human umbilical vein endothelial cells (EAHY926) were used to assess the cytocompatibility of materials. The MWCNTs-COOH displayed the improved cell proliferation, viability and adhesion due to the enhanced wettability, indicating their superior cytocompatibility over MWCNTs. The existence of carboxyl groups should be benefit to the adhesion and growth of both cells, which implied that MWCNTs-COOH were helpful for seeding both cells and could be used as the functional surface for the adhesion and growth of cells.

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First Electrochemical Sensor (Screen-Printed Carbon Electrode Modified with Carboxyl Functionalized Multiwalled Carbon Nanotubes) for Ultratrace Determination of Diclofenac

Materials ◽

10.3390/ma13030781 ◽

2020 ◽

Vol 13 (3) ◽

pp. 781 ◽

Cited By ~ 4

Author(s):

Agnieszka Sasal ◽

Katarzyna Tyszczuk-Rotko ◽

Magdalena Wójciak ◽

Ireneusz Sowa

Keyword(s):

Carbon Nanotubes ◽

Electrochemical Sensor ◽

Multiwalled Carbon Nanotubes ◽

Active Surface Area ◽

Carbon Electrode ◽

Multiwalled Carbon ◽

Screen Printed Carbon Electrode ◽

Functionalized Multiwalled Carbon Nanotubes ◽

Screen Printed

A simple, sensitive and time-saving differential-pulse adsorptive stripping voltammetric (DPAdSV) procedure using a screen-printed carbon electrode modified with carboxyl functionalized multiwalled carbon nanotubes (SPCE/MWCNTs-COOH) for the determination of diclofenac (DF) is presented. The sensor was characterized using optical profilometry, SEM, and cyclic voltammetry (CV). The use of carboxyl functionalized MWCNTs as a SPCE modifier improved the electron transfer process and the active surface area of sensor. Under optimum conditions, very sensitive results were obtained with a linear range of 0.1–10.0 nmol L−1 and a limit of detection value of 0.028 nmol L−1. The SPCE/MWCNTs-COOH also exhibited satisfactory repeatability, reproducibility, and selectivity towards potential interferences. Moreover, for the first time, the electrochemical sensor allows determining the real concentrations of DF in environmental water samples without sample pretreatment steps.

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