scholarly journals Copper Toxicity and Prediction Models of Copper Content in Leafy Vegetables

2019 ◽  
Vol 11 (22) ◽  
pp. 6215 ◽  
Author(s):  
Chiou ◽  
Hsu
Keyword(s):  
Copper Concentration ◽  
Prediction Models ◽  
Ethylenediaminetetraacetic Acid ◽  
Copper Toxicity ◽  
Toxic Metal ◽  
Leafy Vegetables ◽  
Copper Accumulation ◽  
Copper Level ◽  
Yield Reduction ◽  
Water Spinach

Copper (Cu), a toxic metal pollution found in the soil and water of industrialized areas, causes continuous issues for agriculture product contamination and human health hazards. However, information on copper phytotoxicity and its accumulation in vegetables is largely unknown. To evaluate the related agricultural loss and health risks, it is necessary to assess copper phytotoxicity and develop prediction models for copper concentration in vegetables. Here, we assess the growth performance and copper concentration of four leafy vegetables: Water spinach, amaranth, pakchoi, and garland chrysanthemum in copper-contaminated soil. The plant’s height and fresh weight is dramatically reduced when the soil copper concentration is over ~250 mg·kg−1. This yield reduction and copper accumulation are associated with an increase of soil copper concentration, suggesting high copper phytotoxicity levels in plants and soil. The prediction models of plant copper concentration were developed using multiple regressions based on one-step extractions of the soil copper as independent variables. One prediction model derived for amaranth copper using hydrochloric acid (HCl)-extractable and ethylenediaminetetraacetic acid (EDTA)-extractable copper from soil is able to describe 78.89% of the variance in the measured copper. As a result, the phytotoxic copper level for four leafy vegetables is revealed. Although the prediction models may not be universal, the predicted and phytotoxic copper levels are useful tools for evaluating vegetable yield and daily copper intake.

scholarly journals Oxidative stress and neurobehavioural changes in rats following copper exposure and their response to MiADMSA and d-penicillamine

2019 ◽  
Vol 3 ◽  
pp. 239784731984478 ◽  
Author(s):  
Shaheen Quamar ◽  
Jayant Kumar ◽  
Awanish Mishra ◽  
SJS Flora
Keyword(s):  
Oxidative Stress ◽  
Copper Concentration ◽  
Copper Toxicity ◽  
Clinical Feature ◽  
Copper Level ◽  
Copper Exposure ◽  
Muscle Coordination ◽  
Contextual Fear ◽  
Liver Copper ◽  
Ameliorative Effect

An increase in copper concentration in body may lead to hepatolenticular degeneration which is considered as one clinical feature of Wilson’s disease. Chelation therapy using d-penicillamine is the preferred medical treatment for reducing the toxic effects of copper. However, a few shortcomings associated with d-penicillamine led us to search of an alternative antidote for copper toxicity. Monoisoamyl-2, 3-dimercaptosuccinic acid (MiADMSA), a potent arsenic chelator under clinical trial, has been reported to reduce system copper level. Thus, the present study was envisaged to explore the ameliorative effect of MiADMSA against copper toxicity. Copper pre-exposed animals (CuSO4.5H2O; 100 mg/kg; p.o., for 6 weeks) were segregated in different groups and were administered equimolar dose (0.3 mEq/kg/day; p.o.) of d-penicillamine and MiADMSA for 5 days. The effect of different treatments on spontaneous locomotor activity, muscle coordination, depression like behaviour and contextual fear memory was analysed using neurobehavioural battery test. Biochemical variables related to oxidative stress, zinc and copper concentration were determined in liver, kidney and brain. The results suggested that copper exposure led to oxidative stress in liver, kidney and blood, along with moderate effects in brain. Treatment with d-penicillamine and MiADMSA reduced liver copper load. MiADMSA produced more pronounced beneficial effect compared to d-penicillamine by increasing brain GPx activity. Our study suggests that MiADMSA might be equally effective as d-penicillamine in depleting body copper load. More detailed studies using different doses are required to suggest whether MiADMSA could be an alternative for d-penicillamine in reducing oxidative injury, neurobehavioural changes and depleting body copper burden.

Does CSF copper level in Wilson disease reflect copper accumulation in the brain?

1988 ◽  
Vol 4 (1) ◽  
pp. 35-37 ◽  
Author(s):  
Hiroko Kodama ◽  
Ichiro Okabe ◽  
Masayoshi Yanagisawa ◽  
Hiroko Nomiyama ◽  
Kazuo Nomiyama ◽  
...  
Keyword(s):  
Wilson Disease ◽  
Copper Accumulation ◽  
Copper Level ◽  
The Brain

scholarly journals Potential Risk of Consuming Vegetables Planted in Soil with Lead and Cadmium and the Influence on Vegetable Antioxidant Activity

2021 ◽  
Vol 11 (9) ◽  
pp. 3761
Author(s):  
Wen-Lii Huang ◽  
Wei-Hsiang Chang ◽  
Shu-Fen Cheng ◽  
Huai-Yuan Li ◽  
Hsiu-Ling Chen
Keyword(s):  
Sweet Potato ◽  
Contaminated Soils ◽  
Vegetable Consumption ◽  
Accumulation Rate ◽  
Leafy Vegetables ◽  
Water Spinach ◽  
Potato Leaf ◽  
Cd Accumulation ◽  
Lead And Cadmium ◽  
Cd Exposure

Once in soil and water, metals can enter the food chain, and the consumption of contaminated crops can pose a serious risk to human health. This study used pot experiments to evaluate the accumulation of metal elements and their influence on levels of antioxidants in vegetables. The current study clearly demonstrates that metals accumulated in the five vegetables that were planted in the contaminated soils, especially so for water spinach. Cd accumulation of all of the vegetables planted in the contaminated soils was greater Cu. The low accumulation rate that was seen in sweet potato leaf, potato, and tomato indicated their suitability for planting in suspected contaminated soil, such as at farms nearby metal industries, in replacement of high accumulators, such as leafy vegetables. The non-carcinogenic HI of Cd exposure from water spinach and sweet potato were >1, whereas those for Cu were <1. This study suggests that residents may experience health risks due to vegetable consumption, and that children are vulnerable to the adverse effects of heavy metal ingestion.

scholarly journals Nutrient Solution Deprivation as a Tool to Improve Hydroponics Sustainability: Yield, Physiological, and Qualitative Response of Lettuce

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1469
Author(s):  
Michele Ciriello ◽  
Luigi Formisano ◽  
Antonio Pannico ◽  
Christophe El-Nakhel ◽  
Giancarlo Fascella ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Potential Effect ◽  
Quality Parameters ◽  
Leafy Vegetables ◽  
Nitrate Content ◽  
Yield Reduction ◽  
Quality Performance ◽  
Floating Raft ◽  
Amino Acids Profile ◽  
Excessive Nutrients

Hydroponics growing systems often contain excessive nutrients (especially nitrates), which could lead to a quality loss in ready-to-eat leafy vegetables and posing a health risk to consumers, if managed inadequately. A floating raft system was adopted to assay the production and quality performance of lettuce (Lactuca sativa L. cv ‘Maravilla De Verano Canasta’) deprived of the nutrient solution by replacement with only water, three and six days before harvest. Yield and quality parameters, mineral composition, pigments, organic acids, amino acids profile, soluble proteins, and carbohydrate content were determined. Nutrient solution deprivation six days before harvest resulted in a significant reduction in leaf nitrate (−53.3%) concomitant with 13.8% of yield loss, while plants deprived of nutrient solution three days before harvest increased total phenols content (32.5%) and total ascorbic acid (102.1%), antioxidant activity (82.7%), anthocyanins (7.9%), sucrose (38.9%), starch (19.5%), and γ-aminobutyric acid (GABA; 28.2%), with a yield reduction of 4.7%, compared to the control. Our results suggest that nutrient solution deprivation three days before harvest is a successful strategy to reduce nitrate content and increase the nutritional quality of lettuce grown in floating raft systems with negligible impact on yield. These promising results warrant further investigation of the potential effect of nutrient solution deprivation on the quality attributes of other leafy vegetables cultivated in floating raft systems and in a “cascade” growing system.

scholarly journals High-throughput method for detection and quantification of lesions on leaf scale based on trypan blue staining and digital image analysis

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Emina Mulaosmanovic ◽  
Tobias U. T. Lindblom ◽  
Marie Bengtsson ◽  
Sofia T. Windstam ◽  
Lars Mogren ◽  
...  
Keyword(s):  
Image Analysis ◽  
Trypan Blue ◽  
Prediction Models ◽  
Abiotic Factors ◽  
Leafy Vegetables ◽  
Leaf Damage ◽  
Blue Staining ◽  
Economic Losses ◽  
Wounded Area ◽  
Detection And Quantification

Abstract Background Field-grown leafy vegetables can be damaged by biotic and abiotic factors, or mechanically damaged by farming practices. Available methods to evaluate leaf tissue damage mainly rely on colour differentiation between healthy and damaged tissues. Alternatively, sophisticated equipment such as microscopy and hyperspectral cameras can be employed. Depending on the causal factor, colour change in the wounded area is not always induced and, by the time symptoms become visible, a plant can already be severely affected. To accurately detect and quantify damage on leaf scale, including microlesions, reliable differentiation between healthy and damaged tissue is essential. We stained whole leaves with trypan blue dye, which traverses compromised cell membranes but is not absorbed in viable cells, followed by automated quantification of damage on leaf scale. Results We present a robust, fast and sensitive method for leaf-scale visualisation, accurate automated extraction and measurement of damaged area on leaves of leafy vegetables. The image analysis pipeline we developed automatically identifies leaf area and individual stained (lesion) areas down to cell level. As proof of principle, we tested the methodology for damage detection and quantification on two field-grown leafy vegetable species, spinach and Swiss chard. Conclusions Our novel lesion quantification method can be used for detection of large (macro) or single-cell (micro) lesions on leaf scale, enabling quantification of lesions at any stage and without requiring symptoms to be in the visible spectrum. Quantifying the wounded area on leaf scale is necessary for generating prediction models for economic losses and produce shelf-life. In addition, risk assessments are based on accurate prediction of the relationship between leaf damage and infection rates by opportunistic pathogens and our method helps determine the severity of leaf damage at fine resolution.

scholarly journals Application of Waste Lemon Extract to Toxic Metal Removal through Gravitational Soil Flushing and Composting Stabilization

2020 ◽  
Vol 12 (14) ◽  
pp. 5751
Author(s):  
Pei-Wen Zhang ◽  
Ya-Zhen Huang ◽  
Chihhao Fan ◽  
Tsun-Kuo Chang
Keyword(s):  
Citric Acid ◽  
Soil Ph ◽  
Toxic Metals ◽  
Copper Concentration ◽  
Metal Removal ◽  
Toxic Metal ◽  
Extraction Process ◽  
Exchange Reactions ◽  
Citric Acid Concentration ◽  
Soil Flushing

The present study aims to investigate the treatment efficiency of soil flushing using waste lemon extract for samples collected from contaminated farmland, in which the copper concentration was measured as 2487 ± 139 mg/kg. The flushing solution, containing 9.9 g/L citric acid, was prepared from the waste lemon extraction process. The soil-flushing treatment using a solution containing commercial citric acids of 10 g/L was also conducted for comparison. Additionally, the collected soil was mixed with crushed waste lemons and the mixture was subjected to a composting process for subsequent stabilization study. After 120-min batch experiments, the desorbed copper concentration for waste lemon-extract experiment was 36.9 mg/L, which was higher than that (28.6 mg/L) for commercial citric solution experiment. The reduction in soil copper concentration (1504 mg/kg) treated by waste lemon-extract flushing was more than that treated by commercial citric solution (1256 mg/kg) at the comparable citric acid concentration. More metals were removed by waste lemon-extract flushing. This is because the waste lemon-extract solution contains additional co-dissolved organic substances with a longer flushing time, which allows more exchange reactions between adsorbed metals and flushing solution. For the treatment with waste lemon extract, the soil pH values were 4.56, 5.70 and 6.29 before, after flushing and after compost treatment, respectively. The observed variation in soil pH also showed that waste lemon extract might be a better flushing agent, while flushing with commercial citric solution decreased the pH in the soil environment. The plant copper availability dropped from 677 mg/kg to 156 mg/kg after waste lemon-extract flushing and stabilization with composted waste lemon. Therefore, the use of waste lemon extract for soil flushing not only removed toxic metals from the soil but also prevented the occurrence of soil acidification, an often-observed phenomenon using an acidic solution in conventional soil flushing. After soil flushing, the application of composted waste lemon could stabilize the toxic metals and increase the pH to a range suitable for plant growth.

Effect of Copper Toxicity on Root Morphology, Ultrastructure, and Copper Accumulation in Moso Bamboo (Phyllostachys pubescens)

2014 ◽  
Vol 69 (9-10) ◽  
pp. 399-406 ◽  
Author(s):  
Junren Chen ◽  
Danli Peng ◽  
Mohammad Shafi ◽  
Song Li ◽  
Jiasen Wu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Root Morphology ◽  
Copper Toxicity ◽  
Dry Weight ◽  
Moso Bamboo ◽  
Copper Accumulation ◽  
Root Surface Area ◽  
Phyllostachys Pubescens ◽  
Root Tips ◽  
Effect Of Copper

Abstract A hydroponic culture experiment was conducted to study the effect of copper toxicity on root morphology, ultrastructure, and copper accumulation in Moso bamboo (Phyllostachys pubescens). Root ultrastructure of Moso bamboo was studied by transmission electron microscopy and scanning electron microscopy. Application of 200 μM Cu resulted in an accumulation of 810 mg kg-1 dry weight and 91 mg kg-1 dry weight Cu in roots and shoots, respectively. The majority of the plants did not survive the application of 400 μM Cu. Biomass production declined consistently with application of each additional increment of Cu. Root growth was more severely inhibited than shoot growth. Cu adversely affected the root morphology of the plants, however, root surface area and number of root tips increased slightly at low levels of Cu. Root cell ultrastructure and organelles changed significantly under Cu stress, in particular, cell walls, mitochondria, and xylem parenchyma were affected.

scholarly journals Assessment of Lead (Pb) Remediation Potential of Senna obtusifolia in Dareta Village, Zamfara, Nigeria

2020 ◽  
Vol 10 (25) ◽  
pp. 200301
Author(s):  
Udiba Ugumanim Udiba ◽  
Ekpo Eyo Antai ◽  
Ekom Robert Akpan
Keyword(s):  
Environmental Protection Agency ◽  
Translocation Factor ◽  
Toxic Metal ◽  
The United States ◽  
Leafy Vegetables ◽  
Health Concern ◽  
The European Union ◽  
Senna Obtusifolia ◽  
Financial Interests ◽  
The Mean

Background. Environmental contamination by lead (Pb) and other toxic metals is of significant environmental and human health concern. Heavy metals are not readily eliminated by degradation, and thus remediation of contaminated media (soil, sediment and water/sludge) requires the outright removal or cleanup of these metals. Evaluation of the performance and cost efficiency of various remediation methods has led to the development of bioremediation as an inexpensive, innovative and environmentally friendly cleanup strategy. Objectives. The present study was designed to assess the Pb remediation potential of wild Senna obtusifolia (Sicklepod), in Dareta Village, Zamfara, Nigeria. Methods. Soil and Senna obtusifolia samples were collected from established plots and Pb content was determined using a Shimadzu atomic absorption spectrophotometer (model AA-6800, Japan) after wet digestion. Results. The mean concentrations of Pb (mg/kg) in soil, roots, stems and leaves, respectively, were 130.68±5.2, 61.33±17.86, 66.64±18.10 and 173.39±13.73 for plot 1, 287.84±6.5, 69.42±11.62, 123.4±3.67 and 294.28±4.38 for plot 2, 315.73±4.13, 68.42±10.22, 86.89±6.08 and 290.61±7.47 for plot 3, 396.86±5.48, 91.64±2.87, 150.58±2.21 and 282.53±5.69 for plot 4 and 264.23±8.02, 72.71±2.18, 124.60±2.27 and 282.40±3.79 for plot 5. Average values for the translocation factor, bioaccumulation factor and bioconcentration factor were 3.65±0.66, 1.01±0.23 and 0.29±0.10, respectively. Discussion. Soil Pb levels in the present study were found to be within the United States Environmental Protection Agency (USEPA) standards and the Dutch Intervention Values for Pb in soil. Lead content of Senna obtusifolia leaves was found to be higher than the Pb content of the stem and root, indicating relatively low restriction and the efficiency of internal transport of the toxic metal from the roots towards the aerial parts. High translocation and bioaccumulation factors indicate that the plant has vital characteristics for phytoextraction of Pb. The mean Pb concentration of Senna obtusifolia leaves was found to be far above Codex general standards and the European Union (EU) maximum levels for Pb in leafy vegetables. Conclusions. The study concludes that wild Senna obtusifolia has significant characteristics for phytoextraction of Pb and that consumption of Senna obtusifolia leaves from the study area would pose a serious risk of Pb intoxication. Competing Interests. The authors declare no competing financial interests.

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