scholarly journals New Insights into the Toxicokinetics of 3,4-Dichloroaniline in Early Life Stages of Zebrafish (Danio rerio)

Toxics ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 16 ◽  
Author(s):  
Sabrina Schiwy ◽  
Ann-Kathrin Herber ◽  
Henner Hollert ◽  
Markus Brinkmann
Keyword(s):  
Danio Rerio ◽  
High Performance ◽  
Positive Control ◽  
Fish Embryo ◽  
Embryo Toxicity ◽  
Array Detection ◽  
Flow Through ◽  
Multiwell Plates ◽  
Danio Rerio Embryos ◽  
Exposure Conditions

In the fish embryo toxicity (FET) test with zebrafish (Danio rerio) embryos, 3,4-dichloroaniline (3,4-DCA) is often employed as a positive control substance. Previous studies have characterized bioconcentration and transformation of 3,4-DCA in this test under flow-through conditions. However, the dynamic changes of chemical concentrations in exposure media and embryos were not studied systematically under the commonly used semi-static exposure conditions in multiwell plates. To overcome these limitations, we conducted semi-static exposures experiments where embryolarval zebrafish were exposed to 0.5, 2.0, and 4.0 mg L−1 of 3,4-DCA for up to 120 hpf, with 24-h renewal intervals. During each renewal interval, concentrations of 3,4-DCA were quantified in water samples at 0, 6, 18, and 24 h using high-performance liquid chromatography with diode array detection. Levels of 3,4-DCA in larvae were measured after 120 h exposure. Concentrations of 3,4-DCA in the test vessels decreased rapidly during exposure. Taking these dynamics into account, bioconcentration factors in the present study ranged from 12.9 to 29.8 L kg−1, depending on exposure concentration. In summary, this study contributed to our knowledge of chemical dynamics in the FET test with embryolarval zebrafish, which will aid in defining suitable exposure conditions for future studies.

Development of a flow-through system for the fish embryo toxicity test (FET) with the zebrafish (Danio rerio)

2009 ◽  
Vol 23 (7) ◽  
pp. 1436-1442 ◽  
Author(s):  
E. Lammer ◽  
H.G. Kamp ◽  
V. Hisgen ◽  
M. Koch ◽  
D. Reinhard ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Toxicity Test ◽  
Fish Embryo ◽  
Embryo Toxicity Test ◽  
Embryo Toxicity ◽  
Flow Through

scholarly journals Lethal and Sub-Lethal Effects and Modulation of Gene Expression Induced by T Kinase Inhibitors in Zebrafish (Danio Rerio) Embryos

Toxics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 4
Author(s):  
Tina Elersek ◽  
Matjaž Novak ◽  
Mateja Mlinar ◽  
Igor Virant ◽  
Nika Bahor ◽  
...  
Keyword(s):  
Imatinib Mesylate ◽  
Danio Rerio ◽  
Kinase Inhibitors ◽  
Lethal Effects ◽  
Expression Of Genes ◽  
Embryo Toxicity Test ◽  
Damage Response ◽  
Embryo Toxicity ◽  
Modulation Of Gene Expression ◽  
Danio Rerio Embryos

Tyrosine kinase inhibitors (TKIs) are designed for targeted cancer therapy. The consumption of these drugs during the last 20 years has been constantly rising. In the zebrafish (Danio rerio) embryo toxicity test, we assessed the toxicity of six TKIs: imatinib mesylate, erlotinib, nilotinib, dasatinib, sorafenib and regorafenib. Imatinib mesylate and dasatinib induced lethal effects, while regorafenib, sorfenib and dasatinib caused a significant increase of sub-lethal effects, predominantly oedema, no blood circulation and formation of blood aggregates. The analyses of the changes in the expression of selected genes associated with the hormone system after the exposure to imatinib mesylate, dasatinib and regorafenib demonstrated that all three tested TKIs deregulated the expression of oestrogen receptor esr1, cytochrome P450 aromatase (cypa19b) and hydroxysteroid-dehydrogenase (hsd3b), regorafenib, and also thyroglobulin (tg). The expression of genes involved in the DNA damage response (gadd45 and mcm6) and apoptosis (bcl2) was deregulated only by exposure to regorafenib. The data indicate that common mechanisms, namely antiangiogenic activity and interference with steroidogenesis are involved in the TKI induced sub-lethal effects and potential hormone disrupting activity, respectively. The residues of TKIs may represent an environmental hazard; therefore, further ecotoxicological studies focusing also on the effects of their mixtures are warranted.

Sediment-contact fish embryo toxicity assay with Danio rerio to assess particle-bound pollutants in the Tietê River Basin (São Paulo, Brazil)

2011 ◽  
Vol 74 (7) ◽  
pp. 1951-1959 ◽  
Author(s):  
Paula Suares Rocha ◽  
Conny Bernecker ◽  
Ruben Strecker ◽  
Carolina Fiorillo Mariani ◽  
Marcelo Luiz Martins Pompêo ◽  
...  
Keyword(s):  
River Basin ◽  
Danio Rerio ◽  
Sao Paulo ◽  
São Paulo ◽  
Toxicity Assay ◽  
Fish Embryo ◽  
Tietê River ◽  
Embryo Toxicity

scholarly journals Acute toxicity of jalawure (Tacca leontopetaloides (L.) Kuntze) and gadung tikus (Tacca palmata Blume) using zebrafish (Danio rerio) embryos as a model

2021 ◽  
Vol 12 (2) ◽  
pp. 1247-1253
Author(s):  
Agus W ◽  
Suganda A G ◽  
Fidrianny I
Keyword(s):  
Acute Toxicity ◽  
Danio Rerio ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Test Protocol ◽  
Fish Embryo ◽  
Stem Extract ◽  
Danio Rerio Embryos

Tacca is a genus of several species reported to have potential as a new therapeutic agent with toxic properties for several cells. Jalawure and gadung tikus are two species from the Tacca genus that can be found in Indonesia. The aim of this study was to determine the level of acute toxicity of jalawure and gadung tikus by using zebrafish (Danio rerio) embryos as a model. The sample was extracted by maceration using ethanol 96%. The acute toxicity test was carried out by using fish embryo acute toxicity (FET) test protocol No. 236, Organisation for Economic Co-Operation and Development (OECD), 96 hr of static exposure using the negative control group (medium embryo), the test solution group (extract) and the positive control group (3,4-dichloroaniline 4 µg/ml). The result of acute toxicity of extract against zebrafish embryos demonstrated that LC50 of jalawure leaf extract 26.06 ± 0.31 µg/ml, jalawure stem extract 251.52 ± 6.15 µg/ml,  jalawure corm extract 463.24 ± 9.68 µg/ml, gadung tikus leaf extract 15.04 ± 0.15 µg/ml, gadung tikus stem extract 263.73 ± 6.58 µg/ml, and gadung tikus corm extract 17.71 ± 0.12 µg/ml. Embryo coagulation was the endpoint that was most commonly found in almost all extracts. Gadung tikus leaves extract, gadung tikus corm extract and jalawure leaves extract expressed moderate toxicity level.

scholarly journals Tail Coiling Assay in Zebrafish (Danio rerio) Embryos: Stage of Development, Promising Positive Control Candidates, and Selection of an Appropriate Organic Solvent for Screening of Developmental Neurotoxicity (DNT)

Water ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 119
Author(s):  
Andréia A. S. de Oliveira ◽  
Tamires A. V. Brigante ◽  
Danielle P. Oliveira
Keyword(s):  
Organic Solvent ◽  
Dimethyl Sulfoxide ◽  
Danio Rerio ◽  
Positive Control ◽  
Developmental Neurotoxicity ◽  
Stage Of Development ◽  
Activity Measurements ◽  
Experimental Parameters ◽  
Danio Rerio Embryos ◽  
Selection Of

It is relatively recent that tail coiling assay in zebrafish (Danio rerio) embryos has been proposed as an alternative method to screen for developmental neurotoxicity (DNT) induced by chemicals. Despite the considerable use of the method, there is no consensus related to the most suitable age of embryos and other experimental parameters. Non-exposed embryos were videotaped for tail-coiling activity from 18 to 54 h post-fertilization (hpf) and after exposure to positive control candidates (caffeine, fluoxetine, and tricaine (MS-222)) and organic solvents (acetone, dimethyl-sulfoxide (DMSO), and ethanol) from 26.0 to 28.5 hpf. Results demonstrated that embryos from 22 to 29 hpf presented a constant coiling activity, with no significant differences between the activity measurements. We also found that stimulant properties of caffeine and the anesthetic effects of MS-222 induced hyperactivity and hypoactivity, respectively. Finally, even using DMSO at 1%, it seems to be safer as a solvent for neurotoxicity evaluation by tail coiling assay. The period from 26.0 to 28.5 hpf was appropriate for a fast protocol of tail coiling assay. Caffeine and MS-222 were demonstrated to be promising positive control candidates, whereas DMSO was considered the most appropriate solvent choice for tail coiling assay.

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