scholarly journals Soybean Thrips (Thysanoptera: Thripidae) Harbor Highly Diverse Populations of Arthropod, Fungal and Plant Viruses

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1376
Author(s):  
Thanuja Thekke-Veetil ◽  
Doris Lagos-Kutz ◽  
Nancy K. McCoppin ◽  
Glen L. Hartman ◽  
Hye-Kyoung Ju ◽  
...  

Soybean thrips (Neohydatothrips variabilis) are one of the most efficient vectors of soybean vein necrosis virus, which can cause severe necrotic symptoms in sensitive soybean plants. To determine which other viruses are associated with soybean thrips, the metatranscriptome of soybean thrips, collected by the Midwest Suction Trap Network during 2018, was analyzed. Contigs assembled from the data revealed a remarkable diversity of virus-like sequences. Of the 181 virus-like sequences identified, 155 were novel and associated primarily with taxa of arthropod-infecting viruses, but sequences similar to plant and fungus-infecting viruses were also identified. The novel viruses were predicted to have positive-sense RNA, negative-stranded RNA, double-stranded RNA, and single-stranded DNA genomes. The assembled sequences included 100 contigs that represented at least 95% coverage of a virus genome or genome segment. Sequences represented 12 previously described arthropod viruses including eight viruses reported from Hubei Province in China, and 12 plant virus sequences of which six have been previously described. The presence of diverse populations of plant viruses within soybean thrips suggests they feed on and acquire viruses from multiple host plant species that could be transmitted to soybean. Assessment of the virome of soybean thrips provides, for the first time, information on the diversity of viruses present in thrips.

2019 ◽  
Vol 113 (2) ◽  
pp. 949-955 ◽  
Author(s):  
D Lagos-Kutz ◽  
M L Pawlowski ◽  
J Haudenshield ◽  
J Han ◽  
L L Domier ◽  
...  

Abstract Soybean vein necrosis virus (SVNV) was first identified in Arkansas and Tennessee in 2008 and is now known to be widespread in the United States and Canada. Multiple species of thrips transmit this and other tospoviruses with Neohydatothrips variabilis (Beach) (soybean thrips) cited as the most efficient vector for SVNV. In this study, 18 soybean, Glycine max (L.) Merr., genotypes were evaluated in four experiments by infesting plants with noninfected and SVNV-infected thrips using choice and no-choice assays. In both choice experiments with noninfected and SVNV-infected thrips, the lowest number of immature soybean thrips occurred on plant introductions (PIs) 229358 and 604464 while cultivars Williams 82 and Williamsfield Illini 3590N supported higher counts of mature thrips. The counts between the two assays (noninfected and SVNV-infected thrips) were positively correlated. In both no-choice experiments with noninfected and SVNV-infected thrips, counts of thrips did not differ by soybean genotypes. Further studies are needed to characterize the inheritance and mechanisms involved in the resistance found in the choice assay.


2018 ◽  
Vol 8 (3) ◽  
pp. 247-266
Author(s):  
Michelle L. Wilson

Initially, Oliver Twist (1839) might seem representative of the archetypal male social plot, following an orphan and finding him a place by discovering the father and settling the boy within his inheritance. But Agnes Fleming haunts this narrative, undoing its neat, linear transmission. This reconsideration of maternal inheritance and plot in the novel occurs against the backdrop of legal and social change. I extend the critical consideration of the novel's relationship to the New Poor Law by thinking about its reflection on the bastardy clauses. And here, of course, is where the mother enters. Under the bastardy clauses, the responsibility for economic maintenance of bastard children was, for the first time, legally assigned to the mother, relieving the father of any and all obligation. Oliver Twist manages to critique the bastardy clauses for their release of the father, while simultaneously embracing the placement of the mother at the head of the family line. Both Oliver and the novel thus suggest that it is the mother's story that matters, her name through which we find our own. And by containing both plots – that of the father and the mother – Oliver Twist reveals the violence implicit in traditional modes of inheritance in the novel and under the law.


2018 ◽  
Author(s):  
Satish Kodali ◽  
Liangshan Chen ◽  
Yuting Wei ◽  
Tanya Schaeffer ◽  
Chong Khiam Oh

Abstract Optical beam induced resistance change (OBIRCH) is a very well-adapted technique for static fault isolation in the semiconductor industry. Novel low current OBIRCH amplifier is used to facilitate safe test condition requirements for advanced nodes. This paper shows the differences between the earlier and novel generation OBIRCH amplifiers. Ring oscillator high standby leakage samples are analyzed using the novel generation amplifier. High signal to noise ratio at applied low bias and current levels on device under test are shown on various samples. Further, a metric to demonstrate the SNR to device performance is also discussed. OBIRCH analysis is performed on all the three samples for nanoprobing of, and physical characterization on, the leakage. The resulting spots were calibrated and classified. It is noted that the calibration metric can be successfully used for the first time to estimate the relative threshold voltage of individual transistors in advanced process nodes.


Author(s):  
G. O. Hutchinson

The chapter looks at the division between poetry and prose in ancient and other literatures, and shows the importance of rhythmic patterning in ancient prose. The development of rhythmic prose in Greek and Latin is sketched, the system explained and illustrated (from Latin). It is firmly established, for the first time, which of the main Greek non-Christian authors 31 BC–AD 300 write rhythmically. The method takes a substantial sample of random sentence-endings (usually 400) from each of a large number of Imperial authors; it compares that sample with one sample of the same size (400) drawn randomly from a range of authors earlier than the invention of this rhythmic system. A particular sort of X2-test is applied. Many Imperial authors, it emerges, write rhythmically; many do not. The genres most likely to offer rhythmic writing are, unexpectedly, narrative: historiography and the novel.


Author(s):  
Robert Louis Stevenson ◽  
Ian Duncan

Your bed shall be the moorcock’s, and your life shall be like the hunted deer’s, and ye shall sleep with your hand upon your weapons.’ Tricked out of his inheritance, shanghaied, shipwrecked off the west coast of Scotland, David Balfour finds himself fleeing for his life in the dangerous company of Jacobite outlaw and suspected assassin Alan Breck Stewart. Their unlikely friendship is put to the test as they dodge government troops across the Scottish Highlands. Set in the aftermath of the 1745 rebellion, Kidnapped transforms the Romantic historical novel into the modern thriller. Its heart-stopping scenes of cross-country pursuit, distilled to a pure intensity in Stevenson’s prose, have become a staple of adventure stories from John Buchan to Alfred Hitchcock and Ian Fleming. Kidnapped remains as exhilarating today as when it was first published in 1886. This new edition is based on the 1895 text, incorporating Stevenson’s last thoughts about the novel before his death. It includes Stevenson’s ‘Note to Kidnapped’, reprinted for the first time since 1922.


Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1304
Author(s):  
Nicolás Bejerman ◽  
Ralf G. Dietzgen ◽  
Humberto Debat

Rhabdoviruses infect a large number of plant species and cause significant crop diseases. They have a negative-sense, single-stranded unsegmented or bisegmented RNA genome. The number of plant-associated rhabdovirid sequences has grown in the last few years in concert with the extensive use of high-throughput sequencing platforms. Here, we report the discovery of 27 novel rhabdovirus genomes associated with 25 different host plant species and one insect, which were hidden in public databases. These viral sequences were identified through homology searches in more than 3000 plant and insect transcriptomes from the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) using known plant rhabdovirus sequences as the query. The identification, assembly and curation of raw SRA reads resulted in sixteen viral genome sequences with full-length coding regions and ten partial genomes. Highlights of the obtained sequences include viruses with unique and novel genome organizations among known plant rhabdoviruses. Phylogenetic analysis showed that thirteen of the novel viruses were related to cytorhabdoviruses, one to alphanucleorhabdoviruses, five to betanucleorhabdoviruses, one to dichorhaviruses and seven to varicosaviruses. These findings resulted in the most complete phylogeny of plant rhabdoviruses to date and shed new light on the phylogenetic relationships and evolutionary landscape of this group of plant viruses. Furthermore, this study provided additional evidence for the complexity and diversity of plant rhabdovirus genomes and demonstrated that analyzing SRA public data provides an invaluable tool to accelerate virus discovery, gain evolutionary insights and refine virus taxonomy.


1997 ◽  
Vol 75 (6) ◽  
pp. 773-789 ◽  
Author(s):  
Eric J. Dumdei ◽  
Julia Kubanek ◽  
John E. Coleman ◽  
Jana Pika ◽  
Raymond J. Andersen ◽  
...  

Chemical investigations of Cadlinaluteomarginata skin extracts, egg masses, and dietary sponges have led to the identification of the novel terpenoids cadlinaldehyde (30), spongian 32, seco-spongian 35, 20-acetoxy-12-marginatone (38), and lutenolide (39) from the nudibranch skin extracts, the new drimane sesquiterpenoid 1α,2α-diacetoxyalbicanyl acetate (40) from the nudibranch's egg mass, and the new sesquiterpenoids O-methyl-9-oxofurodysininlactone (47), 2-oxomicro-cionin-2-lactone (48), and O-methyl-2-oxomicrocionin-2-lactone (49), from the dietary sponge Pleraplysilla sp. The known terpenoids furodysinin (1), furodysin (16), marginatafuran (21), and 9,11-dihydrogracillin A (37), which have been frequently isolated from C. luteomarginata skin extracts, were found for the first time in extracts of the dietary sponges Pleraplysilla sp. and Aplysilla sp. One of the new terpenoids, cadlinaldehyde (30), has an unprecedented degraded sesterterpenoid skeleton. Keywords: nudibranch, sponge, terpenoids, structure elucidation.


2004 ◽  
Vol 48 (4) ◽  
pp. 1374-1378 ◽  
Author(s):  
Alejandro Beceiro ◽  
Lourdes Dominguez ◽  
Anna Ribera ◽  
Jordi Vila ◽  
Francisca Molina ◽  
...  

ABSTRACT A presumptive chromosomal cephalosporinase (pI, 9.0) from a clinical strain of Acinetobacter genomic species 3 (AG3) is reported. The nucleotide sequence of this β-lactamase shows for the first time the gene encoding an AmpC enzyme in AG3. In addition, the biochemical properties of the novel AG3 AmpC β-lactamase are reported


2002 ◽  
Vol 57 (1) ◽  
pp. 25-42 ◽  
Author(s):  
Peter Rutsch ◽  
Gottfried Huttner

The disodium salt Na2[{(CO)5Cr}2Pb(NO3)2], Na2·1, which contains a lead center in a (4+2) coordination mode, reacts with tetraphenylphosphonium halides [Ph4P]X to give the tetrahedral compounds [Ph4P]2[{(CO)5Cr}2PbX2] (X = Cl: 2a; X = Br: 2b; X = I: 2c). Substitution of the nitrate groups of Na2·1 by alcoxides leads to binuclear compounds of the type [{(CO)5Crg2Pb(μ2-OR)2Pb{Cr(CO)5}2]2- (R = Et: 3a; R = n -Pr: 3b; R = i-Pr: 3c; R = Allyl: 3d). NMR experiments show that these dimeric compounds are in equilibrium with the monomeric species [{(CO)5Cr}2PbR]- .Trialkylphosphanes react with Na2·1 to give the neutral phosphane complexes [{(CO)5Cr}2Pb(PR3)2] (R=Me: 4a; R=Et: 4b; R = n-Bu: 4c), which show dynamic behaviour in solution. All of the novel compounds have been characterized by X-ray analysis, as well as by the usual analytic and spectroscopic techniques. 207Pb-NMR data of Cr(CO)5- bound lead species are reported for the first time.


2020 ◽  
Vol 6 (1) ◽  
pp. 001-012
Author(s):  
Hendro Kuncoro

Bartimaeus:The Amulet of Samarkand is a fantasy fiction novel written by Jonathan Stroud, a British author. In this novel Stroud portrayed the Djinn as an enslaved demon from the perspective of a Djinn character named Bartimaeus. This study was conduct to compare the concept of Djinn found in the novel with the concept of Djinn in Indonesian culture because while reading it for the very first time, the writer felt similarity between those conceptions. Meanwhile, the concept of Djinn in Indonesian culture was formed one of them by the arrival of Islam which had an influence on the development of literature in Indonesia. Among them is the Hikayat/Tale of Tamim Ad-Dari. The conception of the Djinn that can be revealed from the comparison of these two literatures are in the form of aspects of the life of the Djinn, its physical description, its abilities, its diversity, its interaction with humans, and creatures related to the Djinn. A comparative literature is what this study concerns. By comparing two things we will comprehend the contents of these two things. This research is qualitative and descriptive. Literature is not only covers the story, but also express the culture and thought of the author that conveyed through the work he has made. Meanwhile culture is a beliefs, system of language, communication, and practices that share in a certain group of peoples which they use to define their identity. Therefore, it can be said that comparative literature facilitate the cross-cultural and interdisciplinary study of literature. And so, this study emphasis on the analysis of social and cultural production within the cultural differences. Hopefully, this study will have pedagogical benefits from the point of view of two cultures presented, which underlines social and cultural aspect of two cultures for student of literature and also for the further research in the field


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