scholarly journals Investigation of Post Vaccination Reactions of Two Live Attenuated Vaccines against Lumpy Skin Disease of Cattle

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 621
Author(s):  
Zahra Bamouh ◽  
Jihane Hamdi ◽  
Siham Fellahi ◽  
Slimane Khayi ◽  
Mohammed Jazouli ◽  
...  
Keyword(s):  
Skin Disease ◽  
Vaccine Dose ◽  
Neutralizing Antibody ◽  
Skin Lesions ◽  
Whole Body ◽  
Lumpy Skin Disease ◽  
Post Vaccination ◽  
Live Vaccines ◽  
Skin Nodules ◽  
Successful Control

Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. The only method for successful control is early diagnosis and efficient vaccination. Adverse effects of vaccination such as local inflammation at the injection site and localized or generalized skin lesions in some vaccinated animals have been reported with live vaccines. The aim of this work was to compare the safety of two lumpy skin disease (LSD) vaccine strains, Kenyan (Kn) Sheep and Goat Pox (KSGP O-240) and LSDV Neethling (Nt) strain, and to determine the etiology of the post-vaccination (pv) reactions observed in cattle. Experimental cattle were vaccinated under controlled conditions with Nt- and KSGP O-240-based vaccines, using two different doses, and animals were observed for 3 months for any adverse reactions. Three out of 45 cattle vaccinated with LSDV Nt strain (6.7%) and three out of 24 cattle vaccinated with Kn strain (12.5%) presented LSD-like skin nodules, providing evidence that the post-vaccination lesions may not be strain-dependent. Lesions appeared 1–3 weeks after vaccination and were localized in the neck or covering the whole body. Animals recovered after 3 weeks. There is a positive correlation between the vaccine dose and the appearance of skin lesions in vaccinated animals; at the 105 dose, 12% of the animals reacted versus 3.7% at the 104 dose. Both strains induced solid immunity when protection was measured by neutralizing antibody seroconversion.

scholarly journals Occurrences of Lumpy Skin Disease (LSD) in Cattle in Dinajpur Sadar of Bangladesh

2020 ◽  
Vol 7 (3) ◽  
pp. 445-455
Author(s):  
Sumon Sarkar ◽  
Mirza Mienur Meher ◽  
Mst Misrat Masuma Parvez ◽  
Mahfuza Akther
Keyword(s):  
Skin Disease ◽  
Viral Disease ◽  
Skin Lesions ◽  
Young Female ◽  
Whole Body ◽  
Economic Losses ◽  
Local Breed ◽  
Lumpy Skin Disease ◽  
Herd Level ◽  
Level Information

Lumpy skin disease (LSD) is an acute viral disease infectious of cattle and recently emerged very common in Bangladesh causing economic losses. Hence, this study was design to investigate the prevalence of LSD in considering the herd level and some of management status. Thus, a total of 453 sick animals were subjected to study during the period of April 2020 to July 2020 in Dinajpur. LSD was confirmed according to the clinical inspection and microscopic study of skin scraping. The results indicated that the overall prevalence of LSD was 41.06% in cattle. Moreover, the local breed (75%) and young cattle less than one year (64%) were significantly (p<0.001) higher for LSD with the significant (p<0.001) skin lesions in whole body (44%). In addition, the animal grazed in flock (61%), non-dewormed (58%), non-vaccinated (61%) was significantly (p<0.05) higher for LSD. In the same way, 75% prevalence was in without fly repellent (p<0.001). Afterward, the univariate logistic regression in herd level information had the odd ratio of local breed (95% CI: 0.244-0.553), skin lesions in abdominal regions (95% CI: 1.620-5.923) and pregnant cattle (95% CI: 1.057-3.386) was 0.367, 3.098 and 1.892 respectively indicated the likelihood of no LSD outbreaks. Besides this, the odd ratio of dewormed cattle, vaccinated, individually grazed, regular use of disinfectant and fly repellent farm animal was 1.493 (95% CI:1.024-2.177), 1.491 (95% CI:1.020-2.180), 1.656 (95% CI:1.133-2.421), 1.516 (95% CI:0.952-2.414) and 1.660 (95% CI:1.097-2.513), respectively indicated the likelihood of no LSD. Therefore, LSD infection can be greatly reduced by practicing regular vaccination, deworming, and disinfection, vector controlling and allowing grazing individually, especially with great concern to young female cattle of local breed. Res. Agric., Livest. Fish.7(3): 445-455,  December 2020

scholarly journals Lumpy skin disease in southern Africa : a review of the disease and aspects of control

2001 ◽  
Vol 72 (2) ◽  
Author(s):  
P. Hunter ◽  
D. Wallace
Keyword(s):  
South Africa ◽  
Southern Africa ◽  
Skin Disease ◽  
Control Measures ◽  
Lumpy Skin Disease ◽  
Strain Vaccine ◽  
Successful Control

This article reviews some of the important aspects of lumpy skin disease (LSD) that may impact on its successful control. A resurgence of the disease in the last decade has highlighted some constraints of the Neethling strain vaccine, but there is no evidence of vaccine breakdowns owing to the presence of heterologous field strains. More research is needed on epidemiology and transmission of LSD in South Africa to formulate control measures.

scholarly journals The Effect of Vaccination with Live Attenuated Neethling Lumpy Skin Disease Vaccine on Milk Production and Mortality—An Analysis of 77 Dairy Farms in Israel

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 324 ◽  
Author(s):  
Michal Morgenstern ◽  
Eyal Klement
Keyword(s):  
Adverse Effects ◽  
Milk Production ◽  
Skin Disease ◽  
Linear Models ◽  
Viral Disease ◽  
Lumpy Skin Disease ◽  
Post Vaccination ◽  
Efficient Measure ◽  
Daily Milk Production ◽  
Cattle Farms

Lumpy skin disease (LSD) is an economically important, arthropod borne viral disease of cattle. Vaccination by the live attenuated homologous Neethling vaccine was shown as the most efficient measure for controlling LSD. However, adverse effects due to vaccination were never quantified in a controlled field study. The aim of this study was to quantify the milk production loss and mortality due to vaccination against LSD. Daily milk production, as well as culling and mortality, were retrieved for 21,844 cows accommodated in 77 dairy cattle farms in Israel. Adjusted milk production was calculated for each day during the 30 days post vaccination. This was compared to the preceding month by fitting mixed effects linear models. Culling and mortality rates were compared between the 60 days periods prior and post vaccination, by survival analysis. The results of the models indicate no significant change in milk production during the 30 days post vaccination period. No difference was observed between the pre- and post-vaccination periods in routine culling, as well as in immediate culling and in-farm mortality. We conclude that adverse effects due to Neethling vaccination are negligible.

scholarly journals BNT162b2 SARS-CoV-2 Vaccination Elicits High Titers of Neutralizing Antibodies to Both B.1 and P.1 Variants in Previously Infected and Uninfected Subjects

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 896
Author(s):  
Ilaria Vicenti ◽  
Francesca Gatti ◽  
Renzo Scaggiante ◽  
Adele Boccuto ◽  
Daniela Zago ◽  
...  
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Neutralizing Antibodies ◽  
Vaccine Dose ◽  
Neutralizing Antibody ◽  
Post Vaccination ◽  
Care Workers ◽  
Antibody Titers ◽  
Cross Neutralization ◽  
Highly Correlated

We aimed to investigate neutralizing antibody titers (NtAbT) to the P.1 and B.1 SARS-CoV-2 variants in a cohort of healthy health care workers (HCW), including 20 previously infected individuals tested at baseline (BLinf, after a median of 298 days from diagnosis) and 21 days after receiving one vaccine dose (D1inf) and 15 uninfected subjects tested 21 days after the second-dose vaccination (D2uninf). All the subjects received BNT162b2 vaccination. D1inf NtAbT increased significantly with respect to BLinf against both B.1 and P.1 variants, with a fold-change significantly higher for P.1. D1inf NtAbT were significantly higher than D2uninf NtAbT, against B.1 and P.1. NtAbT against the two strains were highly correlated. P.1 NtAbT were significantly higher than B.1 NtAbT. This difference was significant for post-vaccination sera in infected and uninfected subjects. A single-dose BNT162b2 vaccination substantially boosted the NtAb response to both variants in the previously infected subjects. NtAb titers to B.1 and P.1 lineages were highly correlated, suggesting substantial cross-neutralization. Higher titers to the P.1 than to the B.1 strain were driven by the post-vaccination titers, highlighting that cross-neutralization can be enhanced by vaccination.

scholarly journals Complete Coding Sequences of Lumpy Skin Disease Virus Strains Isolated from Cutaneous Lesions in Namibian Cattle during 2016 Outbreaks

2020 ◽  
Vol 9 (28) ◽  
Author(s):  
Elisabetta Di Felice ◽  
Chiara Pinoni ◽  
Siegfried Khaiseb ◽  
Cesare Camma ◽  
Andrea Capobianco Dondona ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Cutaneous Lesions ◽  
Lumpy Skin Disease ◽  
Coding Sequences ◽  
Lumpy Skin Disease Virus ◽  
Skin Nodules ◽  
Virus Isolates ◽  
Virus Strains

ABSTRACT Between September and October 2016, an outbreak of lumpy skin disease (LSD) was monitored in the Okakarara veterinary district of Namibia. The complete coding sequences were obtained for LSD virus isolates from skin nodules from two symptomatic animals.

scholarly journals Dataset of the microbiome composition in skin lesions caused by lumpy skin disease virus via 16s rRNA massive parallel sequencing

Data in Brief ◽  
2019 ◽  
Vol 27 ◽  
pp. 104764
Author(s):  
Sören Hansen ◽  
Rodrigo Pessôa ◽  
Andrezza Nascimento ◽  
Mohamed El-Tholoth ◽  
Ahmed Abd El Wahed ◽  
...  
Keyword(s):  
16S Rrna ◽  
Disease Virus ◽  
Skin Disease ◽  
Skin Lesions ◽  
Massive Parallel Sequencing ◽  
Lumpy Skin Disease ◽  
Parallel Sequencing ◽  
Lumpy Skin Disease Virus ◽  
Microbiome Composition

scholarly journals Comparative study between lumpy skin disease virus and sheep pox virus vaccines against recent field isolate of lumpy skin disease virus

Bionatura ◽  
2021 ◽  
Vol 3 (3) ◽  
pp. 1955-1959
Author(s):  
Nermeen G Shafik ◽  
Heba A Khafagy ◽  
Amal AM ◽  
Ayatollah I Bassiuony ◽  
Farid Fouad Zaki ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Field Isolate ◽  
Challenge Test ◽  
Neutralizing Antibody ◽  
Antigenic Relationship ◽  
Serum Samples ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Vaccine Type

Lumpy Skin Disease (LSD) is a vector born disease of cattle, caused by Lumpy Skin Disease Virus (LSDV), there is antigenic relationship between LSDV, Sheeppox virus (SPPV) and Goat pox virus GTPV within a genus Capripoxvirus, accordingly it can be used homologous or heterologous Capripoxvirus strains for vaccination of cattle against LSD. This study compare the efficacy of live attenuated Neethling LSDV vaccine and live attenuated Romanian SSPV Vaccine against recent circulating LSDV field isolate. The evaluation was done in calves as the main host of LSD, through using three different batches for each vaccine type. Experimental calf groups were vaccinated with vaccines batches, and after 21 days serum samples were collected for evaluation of humoral immune response by using SNT and commercial ELISA technique, then the vaccinated calves were challenged by virulent LSDV field isolate. The results of SNT for vaccinated calves by LSDV vaccines indicated mean neutralizing antibody titer 1.2, 1.6 and1.5 log10 for the batches 1, 2 and 3 respectively, while vaccinated calves by SPPV vaccines indicated 1.05, 1.05 and 1.5 log10 for the batches 1,2 and 3 respectively; the ELISA mean sample to positive (S/P) percentage for the vaccine batches 1, 2 and 3 of LSDV were 40, 45 and 42% respectively and for SPPV vaccine batches 1,2 and 3 were 35, 37 and 40 % respectively, the challenge test indicated mean difference titer for the groups of calves vaccinated with LSDV vaccine were 4.2, 4.5 and 3.8 log10 and for groups vaccinated with SPPV vaccine were 2.6, 2 and 2.65 log10 respectively, it was concluded that potential using of Neethling LSDV vaccine against LSD is superior for combating and prevention of the lumpy skin disease.

scholarly journals Systemic Dermatitis Model Mice Exhibit Atrophy of Visceral Adipose Tissue and Increase Stromal Cells via Skin-Derived Inflammatory Cytokines

2020 ◽  
Vol 21 (9) ◽  
pp. 3367
Author(s):  
Kento Mizutani ◽  
Eri Shirakami ◽  
Masako Ichishi ◽  
Yoshiaki Matsushima ◽  
Ai Umaoka ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Inflammatory Cytokines ◽  
Stromal Cells ◽  
Skin Disease ◽  
Skin Lesions ◽  
The Body ◽  
Whole Body ◽  
Inflammatory Skin Disease ◽  
Tnf Α ◽  
Inflammatory Skin

Adipose tissue (AT) is the largest endocrine organ, producing bioactive products called adipocytokines, which regulate several metabolic pathways, especially in inflammatory conditions. On the other hand, there is evidence that chronic inflammatory skin disease is closely associated with vascular sclerotic changes, cardiomegaly, and severe systemic amyloidosis in multiple organs. In psoriasis, a common chronic intractable inflammatory skin disease, several studies have shown that adipokine levels are associated with disease severity. Chronic skin disease is also associated with metabolic syndrome, including abnormal tissue remodeling; however, the mechanism is still unclear. We addressed this problem using keratin 14-specific caspase-1 overexpressing transgenic (KCASP1Tg) mice with severe erosive dermatitis from 8 weeks of age, followed by re-epithelization. The whole body and gonadal white AT (GWAT) weights were decreased. Each adipocyte was large in number, small in size and irregularly shaped; abundant inflammatory cells, including activated CD4+ or CD8+ T cells and toll-like receptor 4/CD11b-positive activated monocytes, infiltrated into the GWAT. We assumed that inflammatory cytokine production in skin lesions was the key factor for this lymphocyte/monocyte activation and AT dysregulation. We tested our hypothesis that the AT in a mouse dermatitis model shows an impaired thermogenesis ability due to systemic inflammation. After exposure to 4 °C, the mRNA expression of the thermogenic gene uncoupling protein 1 in adipocytes was elevated; however, the body temperature of the KCASP1Tg mice decreased rapidly, revealing an impaired thermogenesis ability of the AT due to atrophy. Tumor necrosis factor (TNF)-α, IL-1β and interferon (INF)-γ levels were significantly increased in KCASP1Tg mouse ear skin lesions. To investigate the direct effects of these cytokines, BL/6 wild mice were administered intraperitoneal TNF-α, IL-1β and INF-γ injections, which resulted in small adipocytes with abundant stromal cell infiltration, suggesting those cytokines have a synergistic effect on adipocytes. The systemic dermatitis model mice showed atrophy of AT and increased stromal cells. These findings were reproducible by the intraperitoneal administration of inflammatory cytokines whose production was increased in inflamed skin lesions.

scholarly journals Lumpy Skin Disease Is Characterized by Severe Multifocal Dermatitis With Necrotizing Fibrinoid Vasculitis Following Experimental Infection

2020 ◽  
Vol 57 (3) ◽  
pp. 388-396
Author(s):  
Beatriz Sanz-Bernardo ◽  
Ismar R. Haga ◽  
Najith Wijesiriwardana ◽  
Philippa C. Hawes ◽  
Jennifer Simpson ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Skin Disease ◽  
Inclusion Bodies ◽  
Ultrastructural Changes ◽  
Full Thickness ◽  
Lumpy Skin Disease ◽  
Intracytoplasmic Inclusion ◽  
Skin Nodules ◽  
Intracytoplasmic Inclusion Bodies ◽  
Over Time

Lumpy skin disease is a high-consequence disease in cattle caused by infection with the poxvirus lumpy skin disease virus (LSDV). The virus is endemic in most countries in Africa and an emerging threat to cattle populations in Europe and Asia. As LSDV spreads into new regions, it is important that signs of disease are recognized promptly by animal caregivers. This study describes the gross, microscopic, and ultrastructural changes that occur over time in cattle experimentally challenged with LSDV. Four calves were inoculated with wildtype LSDV and monitored for 19 to 21 days. At 7 days after inoculation, 2 of the 4 cattle developed multifocal cutaneous nodules characteristic of LSD. Some lesions displayed a targetoid appearance. Histologically, intercellular and intracellular edema was present in the epidermis of some nodules. Occasional intracytoplasmic inclusion bodies were identified in keratinocytes. More severe and consistent changes were present in the dermis, with marked histiocytic inflammation and necrotizing fibrinoid vasculitis of dermal vessels, particularly the deep dermal plexus. Chronic lesions consisted of full-thickness necrosis of the dermis and epidermis. Lesions in other body organs were not a major feature of LSD in this study, highlighting the strong cutaneous tropism of this virus. Immunohistochemistry and electron microscopy identified LSDV-infected histiocytes and fibroblasts in the skin nodules of affected cattle. This study highlights the noteworthy lesions of LSDV and how they develop over time.

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