scholarly journals Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement

2018 ◽  
Vol 5 (4) ◽  
pp. 103
Author(s):  
Marlene Sickinger ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend
Keyword(s):  
Substance P ◽  
Dairy Cows ◽  
Vasoactive Intestinal Polypeptide ◽  
Serum Levels ◽  
Common Disease ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Breed Difference ◽  
Abomasal Displacement

Abomasal displacement (AD) to the left is a common disease in high-yielding dairy cows after parturition. In view of the previously reported changes in tissue neuropeptide concentrations in cows with AD, the primary aim of this study was to evaluate the effect of AD and breed on serum neuropeptide concentrations. For this purpose, blood samples of 33 German Holstein (GH) cows with AD, 36 healthy controls (GH), and 32 healthy German Fleckvieh (GF) cows were collected, and concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin1β (IL-1β) were measured via commercially available ELISA kits. To examine the effect of AD, we compared GH cows with and without AD and observed no significant effects of AD on SP, VIP, or Il-1 β concentrations. To evaluate the effect of breed, we compared healthy GH with healthy GF cows and detected markedly higher VIP serum levels in the healthy GF cows (p < 0.01). No significant differences in SP or IL-1β were detected. According to our results, there seems to be no effect of AD on the serum concentrations of SP, VIP, or IL-1 β. In contrast, there seems to be a breed difference concerning serum VIP concentrations.

scholarly journals Oral Microbiota Composition and Antimicrobial Antibody Response in Patients with Recurrent Aphthous Stomatitis

2019 ◽  
Vol 7 (12) ◽  
pp. 636 ◽  
Author(s):  
Zuzana Stehlikova ◽  
Vojtech Tlaskal ◽  
Natalie Galanova ◽  
Radka Roubalova ◽  
Jakub Kreisinger ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Serum Levels ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Common Disease ◽  
Oral Microbiota ◽  
Healthy Controls ◽  
Alpha And Beta Diversity ◽  
Oral Site ◽  
Bacteria And Fungi

Recurrent aphthous stomatitis (RAS) is the most common disease of the oral mucosa, and it has been recently associated with bacterial and fungal dysbiosis. To study this link further, we investigated microbial shifts during RAS manifestation at an ulcer site, in its surroundings, and at an unaffected site, compared with healed mucosa in RAS patients and healthy controls. We sampled microbes from five distinct sites in the oral cavity. The one site with the most pronounced differences in microbial alpha and beta diversity between RAS patients and healthy controls was the lower labial mucosa. Detailed analysis of this particular oral site revealed strict association of the genus Selenomonas with healed mucosa of RAS patients, whereas the class Clostridia and genera Lachnoanaerobaculum, Cardiobacterium, Leptotrichia, and Fusobacterium were associated with the presence of an active ulcer. Furthermore, active ulcers were dominated by Malassezia, which were negatively correlated with Streptococcus and Haemophilus and positively correlated with Porphyromonas species. In addition, RAS patients showed increased serum levels of IgG against Mogibacterium timidum compared with healthy controls. Our study demonstrates that the composition of bacteria and fungi colonizing healthy oral mucosa is changed in active RAS ulcers, and that this alteration persists to some extent even after the ulcer is healed.

The Monoclonal Antibody-defined CAR-3 Antigen is a Serological Marker Associated with Pancreatic Carcinoma

1988 ◽  
Vol 3 (1) ◽  
pp. 29-35 ◽  
Author(s):  
M. Prat ◽  
E. Medico ◽  
P. Piantino ◽  
S. Bretti ◽  
F.P. Rossini ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chronic Pancreatitis ◽  
Inflammatory Diseases ◽  
High Specificity ◽  
Serum Levels ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Breast Carcinomas ◽  
Chronic Inflammatory Diseases ◽  
Healthy Donors

The monoclonal antibody-defined CARS antigen is a new carcinoma associated marker which is expressed on a mucin-like molecule. Serum concentrations of CARS were assayed in 181 patients with carcinomas of different organs, 20 patients with non-carcinomatous malignancies, 123 patients with inflammatory diseases and 150 healthy controls. Serum levels of CARS were significantly increased in 51% of the patients with pancreatic carcinomas, in 60% of patients with biliary tract carcinomas and in about 15% of the patients with carcinomas of the digestive apparatus. Sera from patients with breast carcinomas were negative, as well as sera from patients with melanomas or sarcomas. CAR-3 values in samples from patients with chronic pancreatitis were constantly negative, as were samples from healthy donors. Significant concentrations of CAR-3 were detected in 20% of the sera from patients with acute pancreatitis and in 15% of the sera from patients with cirrhosis. Because of its high specificity for pancreatic carcinomas compared to chronic pancreatitis, CARS seems a promising marker for distinguishing between neoplastic and chronic inflammatory diseases of the pancreas, whose differential diagnosis is difficult.

scholarly journals Association of bovine uterine involution disturbances with serum neuropeptide concentrations

2020 ◽  
Vol 13 (9) ◽  
pp. 1854-1857
Author(s):  
Aida Abultdinova ◽  
Isatay Jakupov ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Levels ◽  
Elisa Test ◽  
Normative Values ◽  
Uterine Disease ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Uterine Involution ◽  
Significant Difference

Background and Aim: Puerperal diseases influence fertility and should be diagnosed as soon as possible. This study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin (IL)1β in the early diagnosis of uterine involution disturbances. Materials and Methods: Blood serum samples of 86 dairy cows from six different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP, and IL-1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease. Results: SP concentrations increased significantly within 20 days after calving (p<0.04) with no significant difference observed between the groups. Moreover, no significant differences were found between VIP and log IL-1β. Conclusion: Results showed that none of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded. Further research is needed as regards the establishment of normative values concerning this parameter.

scholarly journals Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients

2015 ◽  
Vol 84 (1) ◽  
pp. 34-40
Author(s):  
Anna Olewicz-Gawlik ◽  
Izabela Korczowska-Łącka ◽  
Paweł Hrycaj
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Acute Phase Proteins ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Male And Female ◽  
Leukocyte Extravasation

Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation. Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients. Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA). Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p < 0.001) and significantly correlated with serum concentrations of rheumatoid factor (RF) and antibodies against cyclic citrullinated peptides (ACPA) (p = 0.006, p = 0.04, respectively). Moreover, we found significant correlations between the serum levels of soluble (s)P- and sE-selectin and ACT-FR (p = 0.008 and p = 0.03, respectively) only in male RA patients.Conclusions. Fucosylation of ACT differs between male and female RA patients and is related to sP- and sE-selectin levels only in men.

scholarly journals Investigations on the Effects of Different Calcium Supply Exceeding the Requirements on Mineral Serum Concentrations and Bone Metabolism in Young Warmblood Stallions

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2439
Author(s):  
Dana Carina Schubert ◽  
Lisa-Theresa Neustädter ◽  
Manfred Coenen ◽  
Christian Visscher ◽  
Josef Kamphues
Keyword(s):  
Trace Elements ◽  
Bone Metabolism ◽  
Bone Markers ◽  
Serum Levels ◽  
Serum Concentrations ◽  
Feeding Trial ◽  
Blood Samples ◽  
Mineral Supplements ◽  
High Calcium ◽  
Calcium Supply

Since mineral supplements for horses commonly contain macro minerals, although the requirement for such is usually covered by roughage-based diets, the aim of this study was to investigate the effects of different dietary calcium levels on mineral serum concentrations and bone metabolism. The trial was conducted in 30 young warmblood stallions (2–3 years) that were divided into two groups for a five-month feeding trial. The groups were fed a hay- and oat-based diet and were either supplied with high (Ca-High) or moderate (Ca-Moderate) calcium excess. While in Ca-High calcium supply was about 2–2.5-fold of the requirement, in Ca-Moderate calcium requirements were slightly surpassed (1.5–1.6-fold). In order to monitor the effects of the different calcium supply, blood samples were taken during the trial and analysed for levels of macro and trace elements as well as concentrations of two bone markers. In Ca-Moderate a trend towards higher phosphorus serum levels compared to Ca-High was observed which was significant at the end of the trial (p = 0.0002). Furthermore, results showed no influence of the diet on bone markers. Results support the idea that forage-based rations for horses do not necessarily have to be supplemented with macro minerals but with trace elements.

scholarly journals The effect of surgery on the levels of matrix metalloproteinases in patients with inguinal hernia

2021 ◽  
Vol 70 (4) ◽  
pp. 627-634
Author(s):  
Sabina Strohalmová ◽  
Kateřina Levová ◽  
Aleš Antonín Kuběna ◽  
Zdeněk Krška ◽  
David Hoskovec ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Inguinal Hernia ◽  
Surgical Treatment ◽  
Matrix Metalloproteinases ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Metalloproteinase Inhibitors ◽  
Before And After ◽  
Circulating Levels

Matrix metalloproteinases (MMPs) are associated with the alteration of extracellular matrix. The purpose of this study was to investigate how the levels of matrix metalloproteinases and their inhibitors – TIMPs are influenced by the presence of inguinal hernia as well as by its surgical treatment. The studied group consisted of 25 patients with inguinal hernia and 21 healthy controls for comparison. Two blood samples - before and after the treatment were collected from patients. Serum concentrations of MMPs and TIMPs were analysed by multiplex immunoassays. There was a difference in circulating levels of MMPs in patients before the surgery compared to healthy controls – the concentrations of MMP-2 and MMP-9 were significantly lower (p=0.026, p=0.018, respectively). After the surgery, the levels of MMPs, especially MMP-2 (p<0.0001), were significantly decreased in patients compared to the preoperative values, apart from MMP 9. On the contrary, MMP-9 showed significant increase after the surgery (p<0.0001). Circulation levels of TIMP-2 in patients were significantly decreased in comparison with controls (p=0.004), whereas levels of TIMP-1 were similar to controls. Both tested metalloproteinase inhibitors showed a significant decrease in detected levels (TIMP-1 p=0.0004; TIMP-2 p<0.0001) after the procedure compared to the preoperative values. The levels of MMPs, especially MMP-2 and MMP-9, and their inhibitors TIMP-1 and TIMP-2 are involved by the presence of inguinal hernia as well as are influenced by the surgery.

Traditional Chinese Whole-Body Acupuncture Mobilizes CD133(+)CD34(−) Cells

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5405-5405
Author(s):  
Sonja Moldenhauer ◽  
Miriam Burgauner ◽  
Rainer Hellweg ◽  
Andreas Lun ◽  
Holger Kiesewetter ◽  
...  
Keyword(s):  
Serum Levels ◽  
Whole Body ◽  
Granulocyte Colony Stimulating Factor ◽  
Serum Concentrations ◽  
Spinal Cord Lesions ◽  
Blood Samples ◽  
Cd34 Cells ◽  
Body Acupuncture ◽  
Study Participants ◽  
Stimulating Factor

Abstract The objective of this study was to analyze the effects of traditional Chinese acupuncture for spinal cord lesions on the mobilization of stem cells. Therefore, 14 healthy study participants were acupunctured and gave blood samples before, immediately after as well as 24 and 48 hours after acupuncture. At these time points, the frequency of CD133, CD34, CD4, CD14, CD19 and CD45 positive cells were determined by flow cytometry. Furthermore, serum concentrations of matrix metalloproteinases (MMP) 8 and 9, brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), stroma-derived factor (SDF) 1 and granulocyte-colony stimulating factor (G-CSF) were measured by enzyme-linked immunosorbent assays. CD133(+)34(−) cells were significantly increased 48 hours after acupuncture (29.1±5.8% versus 11.4±4.8%, p= 0.015), which was paralleled by significant decreases of BDNF (7.5±1 ng/ml versus 10.8±1.6 ng/ml, p= 0.013) and MMP-9 serum levels (46.4±2.6ng/ml versus 42.5±2.4 ng/ml, p= 0.009). Values in non-treated controls were not affected. No changes in SDF-1, NGF, interleukin-6 and G-CSF concentrations occurred. In conclusion, acupuncture does mobilize non-hematopoietic CD133(+) cells.

scholarly journals Metabolic Profile and Hormonal Status Comparison Between Primiparous and Multiparous Non-Cyclic Cows

2018 ◽  
Vol 41 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Besir Jasari ◽  
Branko Atanasov ◽  
Irena Celeska ◽  
Miroslav Radeski ◽  
Martin Nikolovski ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Total Protein ◽  
Metabolic Profile ◽  
Transrectal Ultrasonography ◽  
Hormonal Status ◽  
Metabolic Profiles ◽  
Serum Concentrations ◽  
Physiological Concentration ◽  
Blood Samples ◽  
Luteal Tissue

Abstract Several reports indicated that a large proportion of dairy cows have not resumed cyclicity until day 60 after calving. These cows are traditionally classified as non-cycling (anoestrous or anovular cows). Static ovaries (SO, lack of luteal tissue and follicles >8 mm, and progesterone < 0.5 ng/mL) could be a possible underlying reason that contributes to a non-cycling status. Although SO affects both primiparous (PP) and multiparous (MP) cows, PP cows are more prone to be non-cycling than MP. Therefore, this study aims to compare the metabolic profiles and hormonal status between non-cycling PP and MP cows diagnosed with SO. One hundred and twenty one animals that did not express signs of oestrus until day 60 postpartum were grouped by parity (PP, n=58 and MP, n=63), then blood sampled and examined using transrectal ultrasonography. Blood samples were collected before the ultrasonographic examination. Out of those, 42 PP (72.4%) and 28 MP (44.4%) were diagnosed as non-cycling (bearing SO). Serum concentrations of triglycerides, cholesterol, total protein and albumin did not differ between parity groups. The glucose concentrations in PP cows (1.43 ± 0.59 mmol/L) and MP cows (1.69 ± 0.71 mmol/L) did not differ, however, they were less than the normal physiological concentration. In addition, no differences were detected between parity groups for concentrations of NEFA, β-HBA, progesterone and estradiol. In summary, we concluded that non-cycling PP and MP cows bearing SO have similar hormonal status and metabolic profiles.

scholarly journals Association of bovine uterine involution disturbances with serum neuropeptide concentrations

2019 ◽  
Author(s):  
Aida Abultdinova ◽  
Issatay Jakupov ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Levels ◽  
Elisa Test ◽  
Normative Values ◽  
Uterine Disease ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Uterine Involution ◽  
Significant Difference

Abstract Background: Puerperal diseases influence fertility and should be diagnosed as soon as possible. The present study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP) and IL‑1β in the early diagnosis of uterine involution disturbances. Blood serum samples of 86 dairy cows from 6 different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP and IL‑1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease. Results: SP-concentrations increased significantly within 20 days after calving (P < 0.04) with no significant difference between the groups. No significant differences could be shown for VIP and logIL-1β. Conclusion: None of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded though. Further research is needed with regard to the establishment of normative values concerning this parameter.

A central action of α-melanocyte-stimulating hormone on serum levels of LH and prolactin in rats

1990 ◽  
Vol 124 (1) ◽  
pp. 127-132 ◽  
Author(s):  
T. Scimonelli ◽  
M. E. Celis
Keyword(s):  
Dopaminergic System ◽  
Single Injection ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Ovariectomized Rats ◽  
Serum Concentrations ◽  
Prolactin Release ◽  
Blood Samples ◽  
Melanocyte Stimulating Hormone ◽  
Tuberoinfundibular Dopaminergic System

ABSTRACT We have investigated the effect of administration of α-MSH into the median eminence (ME) of rats on the release of LH and prolactin. Continuous infusion of α-MSH (0·5 μg/h) into the ME from the afternoon of the second day of dioestrus and over the 24 h of pro-oestrus inhibited the preovulatory LH and prolactin surge and the occurrence of ovulation. This inhibitory effect on LH and prolactin release was also observed in chronically ovariectomized rats given a single injection of α-MSH (1 μg/μl per rat) into the ME (blood samples were collected 0,20,60,90,105 and 120 min after injection). The intraperitoneal injection of the dopamine receptor blocker, haloperidol (2 mg/kg), 30 min before the injection of α-MSH into the ME prevented the inhibitory effect of α-MSH on the release of LH and prolactin. These results suggest that hypothalamic α-MSH might be involved in the regulation of LH and prolactin release via the tuberoinfundibular dopaminergic system and that this system also modifies the serum concentrations of α-MSH. Journal of Endocrinology (1990) 124, 127–132

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