scholarly journals Investigations on the Effects of Different Calcium Supply Exceeding the Requirements on Mineral Serum Concentrations and Bone Metabolism in Young Warmblood Stallions

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2439
Author(s):  
Dana Carina Schubert ◽  
Lisa-Theresa Neustädter ◽  
Manfred Coenen ◽  
Christian Visscher ◽  
Josef Kamphues
Keyword(s):  
Trace Elements ◽  
Bone Metabolism ◽  
Bone Markers ◽  
Serum Levels ◽  
Serum Concentrations ◽  
Feeding Trial ◽  
Blood Samples ◽  
Mineral Supplements ◽  
High Calcium ◽  
Calcium Supply

Since mineral supplements for horses commonly contain macro minerals, although the requirement for such is usually covered by roughage-based diets, the aim of this study was to investigate the effects of different dietary calcium levels on mineral serum concentrations and bone metabolism. The trial was conducted in 30 young warmblood stallions (2–3 years) that were divided into two groups for a five-month feeding trial. The groups were fed a hay- and oat-based diet and were either supplied with high (Ca-High) or moderate (Ca-Moderate) calcium excess. While in Ca-High calcium supply was about 2–2.5-fold of the requirement, in Ca-Moderate calcium requirements were slightly surpassed (1.5–1.6-fold). In order to monitor the effects of the different calcium supply, blood samples were taken during the trial and analysed for levels of macro and trace elements as well as concentrations of two bone markers. In Ca-Moderate a trend towards higher phosphorus serum levels compared to Ca-High was observed which was significant at the end of the trial (p = 0.0002). Furthermore, results showed no influence of the diet on bone markers. Results support the idea that forage-based rations for horses do not necessarily have to be supplemented with macro minerals but with trace elements.

scholarly journals Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement

2018 ◽  
Vol 5 (4) ◽  
pp. 103
Author(s):  
Marlene Sickinger ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend
Keyword(s):  
Substance P ◽  
Dairy Cows ◽  
Vasoactive Intestinal Polypeptide ◽  
Serum Levels ◽  
Common Disease ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Breed Difference ◽  
Abomasal Displacement

Abomasal displacement (AD) to the left is a common disease in high-yielding dairy cows after parturition. In view of the previously reported changes in tissue neuropeptide concentrations in cows with AD, the primary aim of this study was to evaluate the effect of AD and breed on serum neuropeptide concentrations. For this purpose, blood samples of 33 German Holstein (GH) cows with AD, 36 healthy controls (GH), and 32 healthy German Fleckvieh (GF) cows were collected, and concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin1β (IL-1β) were measured via commercially available ELISA kits. To examine the effect of AD, we compared GH cows with and without AD and observed no significant effects of AD on SP, VIP, or Il-1 β concentrations. To evaluate the effect of breed, we compared healthy GH with healthy GF cows and detected markedly higher VIP serum levels in the healthy GF cows (p < 0.01). No significant differences in SP or IL-1β were detected. According to our results, there seems to be no effect of AD on the serum concentrations of SP, VIP, or IL-1 β. In contrast, there seems to be a breed difference concerning serum VIP concentrations.

Effect of proteinate or sulphate mineral sources on trace elements in blood and liver of piglets

2000 ◽  
Vol 71 (1) ◽  
pp. 131-139 ◽  
Author(s):  
S. Schiavon ◽  
L. Bailoni ◽  
M. Ramanzin ◽  
R. Vincenzi ◽  
A. Simonetto ◽  
...  
Keyword(s):  
Trace Elements ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Plasma Levels ◽  
Natural Food ◽  
Blood Samples ◽  
Mineral Supplements ◽  
The Common ◽  
Cu And Zn

AbstractFour hundred piglets were housed in 20 pens and offered for 42 days a pre-starter and then a starter compound supplemented with trace elements given as sulphates (SULF) or proteinates (PROT) at a common level (100) or at a reduced level (20) of inclusion. The common level supplied 278, 148, 315 and 98 mg/kg and the reduced level supplied 128, 38, 135 and 50 mg/kg of iron (Fe), copper (Cu), zinc (Zn) and manganese (Mn), respectively, taking into account the natural food contents. Proteinates used in the trial were analysed and described in terms of content and quality of different potential ligands. Piglet growth was not affected by any treatment. At the end of the trial blood samples were collected from eight pigs for each treatment. These animals were slaughtered and their livers were removed, weighed and analysed. Compared with SULF, PROT increased significantly plasma levels of Fe (25·1 v. 15·7 μmol/l), haemoglobin (10·9 v. 10·4 g/dl) and the number of red blood cells (6·4 v. 6·1 millions per μl) but the liver recovery of Fe was not affected by any treatment. In piglets receiving PROT the liver content of Cu and Zn increased significantly compared with those receiving SULF. On reducing the dosage, Cu in the liver significantly decreased with SULF but not with PROT and the amount of Zn decreased more with SULF than with PROT. The results may reflect a better availability of Cu and Zn when proteinates rather than sulphates were used as mineral supplements.

Traditional Chinese Whole-Body Acupuncture Mobilizes CD133(+)CD34(−) Cells

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5405-5405
Author(s):  
Sonja Moldenhauer ◽  
Miriam Burgauner ◽  
Rainer Hellweg ◽  
Andreas Lun ◽  
Holger Kiesewetter ◽  
...  
Keyword(s):  
Serum Levels ◽  
Whole Body ◽  
Granulocyte Colony Stimulating Factor ◽  
Serum Concentrations ◽  
Spinal Cord Lesions ◽  
Blood Samples ◽  
Cd34 Cells ◽  
Body Acupuncture ◽  
Study Participants ◽  
Stimulating Factor

Abstract The objective of this study was to analyze the effects of traditional Chinese acupuncture for spinal cord lesions on the mobilization of stem cells. Therefore, 14 healthy study participants were acupunctured and gave blood samples before, immediately after as well as 24 and 48 hours after acupuncture. At these time points, the frequency of CD133, CD34, CD4, CD14, CD19 and CD45 positive cells were determined by flow cytometry. Furthermore, serum concentrations of matrix metalloproteinases (MMP) 8 and 9, brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), stroma-derived factor (SDF) 1 and granulocyte-colony stimulating factor (G-CSF) were measured by enzyme-linked immunosorbent assays. CD133(+)34(−) cells were significantly increased 48 hours after acupuncture (29.1±5.8% versus 11.4±4.8%, p= 0.015), which was paralleled by significant decreases of BDNF (7.5±1 ng/ml versus 10.8±1.6 ng/ml, p= 0.013) and MMP-9 serum levels (46.4±2.6ng/ml versus 42.5±2.4 ng/ml, p= 0.009). Values in non-treated controls were not affected. No changes in SDF-1, NGF, interleukin-6 and G-CSF concentrations occurred. In conclusion, acupuncture does mobilize non-hematopoietic CD133(+) cells.

scholarly journals Experimental caprine coccidiosis: the pattern of changes in antioxidant micronutrients and vitamins

2014 ◽  
Vol 4 (2) ◽  
Author(s):  
Ehsan Rakhshandehroo ◽  
Seyed Mostafa Razavi ◽  
Saeed Nazifi
Keyword(s):  
Trace Elements ◽  
Serum Level ◽  
Vitamin C ◽  
Oxidative Damage ◽  
Post Infection ◽  
Pathogenic Species ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Antioxidant Agents ◽  
The Status

This study aimed at investigating the status of non-enzymatic antioxidant agents during experimental caprine coccidiosis. A total of 20 newborn kids were selected and allocated into 2 (diseased and healthy) groups. Ten of the kids were infected with sporulated oocysts of the most pathogenic species of <em>Eimeria</em> and ten served as controls. Blood samples were taken at 0 (before inoculation), 3, 7, 14, 21, 28 and 35 days post infection (dpi) and the concentrations of antioxidant trace elements, antioxidant vitamins and ceruloplasmin were measured. Our data showed remarkable reductions in serum concentrations of some antioxidant trace elements (zinc, manganese and selenium) and vitamins (vitamin C) in the diseased kids, however, significant increases were observed in the serum level of ceruloplasmin in infected animals. These alterations became more prominent at 14 to 21 dpi. These observations suggest that <em>Eimeria</em> parasites can significantly interfere with the levels of some antioxidant trace elements and vitamins during caprine coccidiosis. These changes indicate the overproduction of oxidative radicals during the pathogenesis of <em>Eimeria</em> species that may account for extensive oxidative damage in infected animals.

Biochemical markers of bone turnover during pregnancy in horses: a longitudinal study

2012 ◽  
Vol 15 (4) ◽  
pp. 793-795 ◽  
Author(s):  
C. Greiner ◽  
E. Cavalier ◽  
B. Remy ◽  
A. Gabriel ◽  
F. Farnir ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Biochemical Markers ◽  
Type I Collagen ◽  
Venous Blood ◽  
Cross Linking ◽  
Type I ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Markers Of Bone Turnover ◽  
Carboxy Terminal

Abstract The effect of pregnancy on bone metabolism was investigated in healthy mares. Venous blood samples were collected 7 times from 19 multiparous mares starting at 20-weeks pre-parturition, continuing 6 times in 4-week intervals, including the week of parturition and one week after parturition. Serum concentrations of osteocalcin (OC) and carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I) were determined. Measurement cycles and age had a significant (p < 0.01) influence on OC and CTX-I values. Pregnancy influenced bone metabolism with peak bone formation and resorption values around the time of parturition.

A central action of α-melanocyte-stimulating hormone on serum levels of LH and prolactin in rats

1990 ◽  
Vol 124 (1) ◽  
pp. 127-132 ◽  
Author(s):  
T. Scimonelli ◽  
M. E. Celis
Keyword(s):  
Dopaminergic System ◽  
Single Injection ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Ovariectomized Rats ◽  
Serum Concentrations ◽  
Prolactin Release ◽  
Blood Samples ◽  
Melanocyte Stimulating Hormone ◽  
Tuberoinfundibular Dopaminergic System

ABSTRACT We have investigated the effect of administration of α-MSH into the median eminence (ME) of rats on the release of LH and prolactin. Continuous infusion of α-MSH (0·5 μg/h) into the ME from the afternoon of the second day of dioestrus and over the 24 h of pro-oestrus inhibited the preovulatory LH and prolactin surge and the occurrence of ovulation. This inhibitory effect on LH and prolactin release was also observed in chronically ovariectomized rats given a single injection of α-MSH (1 μg/μl per rat) into the ME (blood samples were collected 0,20,60,90,105 and 120 min after injection). The intraperitoneal injection of the dopamine receptor blocker, haloperidol (2 mg/kg), 30 min before the injection of α-MSH into the ME prevented the inhibitory effect of α-MSH on the release of LH and prolactin. These results suggest that hypothalamic α-MSH might be involved in the regulation of LH and prolactin release via the tuberoinfundibular dopaminergic system and that this system also modifies the serum concentrations of α-MSH. Journal of Endocrinology (1990) 124, 127–132

scholarly journals Kisspeptin serum levels in acute myocardial infarction patients and healthy individuals

2018 ◽  
Vol 5 (3) ◽  
pp. 2111-2118 ◽  
Author(s):  
Mohammad Shojaei ◽  
Saeideh Erfanian ◽  
Abdolreza Sotoodeh Jahromi ◽  
Naeimeh Yusefi ◽  
Abdolhossein Madani ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Serum Levels ◽  
Serum Concentrations ◽  
Healthy Individuals ◽  
Control Groups ◽  
Blood Samples ◽  
St Segment Elevation ◽  
St Segment ◽  
And Control

Introduction: Acute myocardial infarction (AMI) is a leading cause of mortality worldwide for which several risk factors have been identified. Kisspeptin is a multifunctional peptide observed in atherosclerotic plaques. This study aimed to compare kisspeptin serum levels in AMI patients and healthy individuals. Methods: This case-control study was conducted on patients visiting the Cardiology department of Peymanieh Hospital, Jahrom University of Medical Sciences, Iran. Participants were randomly divided into a case and a control groups; the former consisting of 90 AMI patients and the latter of 90 healthy individuals with no cardiac diseases. Blood samples were collected from the participants who also completed the given questionnaire. The enzyme-linked immunosorbent assay (ELISA) test was used to determine kisspeptin levels following serum separation from collected blood samples. Data analysis was performed in SPSS-19 using descriptive (mean and standard deviation) and analytical (The Mann-Whitney U test) statistical tests. Results: The mean age of treatment and control groups was 60.91 and 61.72 years, respectively. The majority (73.3%) of participants in this study were men. Kisspeptin serum concentrations were demonstrated to be significantly lower in the case group than the control group (P<0.001). The results of Mann-Whitney U test revealed that kisspeptin serum concentrations of case and control groups did not have a significant relationship with such variables as gender, smoking, and ST-segment elevation and non-ST-segment elevation (P>0.05). Conclusion: The results revealed that kisspeptin serum concentrations of AMI patients were significantly lower than those of healthy individuals. Accordingly, kisspeptin deficiency appears to be a risk factor for AMI. However, due to the multifunctional nature of this peptide, further studies are necessary to discover its precise mechanism of action.

scholarly journals Physical study of Nickel Serum Level in blood samples from Iraqi populations

2020 ◽  
Vol 12 (01) ◽  
pp. 9-14
Author(s):  
Fatimaa Abbas Shaker ◽  
◽  
Shaymaa Awad Kadhim Alshebly ◽  
Keyword(s):  
Lung Cancer ◽  
Trace Elements ◽  
Serum Level ◽  
Statistical Significance ◽  
Absorption Spectrometry ◽  
Serum Levels ◽  
Blood Samples ◽  
Physical Study ◽  
The Mean ◽  
Serum Components

Trace elements examining of basic biological components in the human and animal body, is essential due to the increase and the decrease in them may lead to toxicity. In this study, 32 patients who were diagnosed with lung cancer in 32 healthy volunteers were compared. Serum level concentrations of Fe trace elements were measured using Atomic Absorption Spectrometry. There was statistical significance difference in nickel serum levels when comparing the mean of healthy with patient concentration, where the value of P (0.05 > p) was found. The level of serum components in the patient may be important in causing lung cancer.

Changes in growth rate and thyroid- and sex-hormones blood levels in rats under sub-chronic lithium treatment

2006 ◽  
Vol 25 (5) ◽  
pp. 243-250 ◽  
Author(s):  
M S Allagui ◽  
N Hfaiedh ◽  
C Vincent ◽  
F Guermazi ◽  
J-C Murat ◽  
...  
Keyword(s):  
Growth Rate ◽  
Lithium Treatment ◽  
Lithium Carbonate ◽  
Serum Levels ◽  
Antagonistic Effect ◽  
Blood Levels ◽  
Vaginal Mucosa ◽  
Dependent Manner ◽  
Serum Concentrations ◽  
Dose Dependent

Lithium therapy, mainly used in curing some psychiatric diseases, is responsible for numerous undesirable side effects. The present study is a contribution to the understanding of the pathophysiological mechanisms underlying lithium toxicity. Male and female mature rats were divided into three batches and fed commercial pellets: one batch was the control and the second and third batches were given 2 g (Li1) and 4 g (Li2) of lithium carbonate/kg of food/day, respectively. After 7, 14, 21 and 28 days, serum levels of free tri-iodothyronine (FT3), thyroxine (FT4), testosterone and estradiol were measured. Attention was also paid to growth rate and a histological examination of testes or vaginal mucosa was carried out. In treated rats, a dose-dependent loss of appetite and a decrease in growth rate were observed, together with symptoms of polydypsia, polyuria and diarrhea. Lithium serum concentrations increased from 0.44 mM (day 7) to 1.34 mM (day 28) in Li1 rats and from 0.66 to 1.45 mM (day 14) in Li2 rats. Li2 treatment induced a high mortality after 14 days, reaching 50-60% in female and male animals. From these data, the LD50 (14 days Li2 chronic treatment) was calculated to be about 0.3 g/day per kilogram of animal, leading to Li serum concentrations of about 1.4 mM. A significant decrease of FT3 and FT4 was observed in treated rats. This effect appeared immediately for the highest dose and was more pronounced for FT3, resulting in an increase of the FT4/FT3 ratio. In males, testosterone decreased and spermatogenesis was stopped. Conversely, in females, estradiol increased in a dose-dependent manner as the animals were blocked in the diestrus phase at day 28. This finding supports a possible antagonistic effect of lithium on the estradiol receptors.

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