The effect of laser phoresis of biologically active substances on microcirculation in paravertebral muscles in the radicular syndrome of the lumbosacral spine

2021 ◽  
pp. 72-76
Author(s):  
Nataliya Borisovna Korchazhkina ◽  
Ruslan Vladimirovich Kupeev
Keyword(s):  
Therapeutic Effect ◽  
Pathological Process ◽  
Main Group ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Control Group ◽  
Lumbosacral Spine ◽  
Paravertebral Muscles ◽  
Radicular Syndrome ◽  
Active Substances

In the case of radicular syndrome in patients with dorsopathy, degenerative dystrophic changes, which contribute to the creation of additional mechanisms of pain impulse and chronization of the pathological process, are diagnosed in the deep muscles. The study included 40 patients with dorsopathy of the lumbosacral spine and radicular syndrome, with the average age of 45,3±1,5years, who were divided into 2 groups comparable in age and clinical and functional characteristics: the main group of 20 patients who were treated with laser phoresis of biologically active substances and the control group of 20 patients who received standard pharmacotherapy. As a result of the study, a significantly more pronounced improvement in the state of microcirculation in the paravertebral muscles when using phytoextracts laser phoresis was proved, which was confirmed by radiothermometry and transcutaneous oximetry. The analysis of radiothermometry and oximetry parameters obtained 3 months after the end of the course showed the stability of the therapeutic effect in 96% of patients in the main group, while in the control group, 50% of patients showed a regression of the effect obtained as a result of treatment. In longer terms, the therapeutic effect in 90% of patients of the main group was maintained for 9–12 months of follow-up, while in the control group — no more than 3–4 months.

OPTIMIZATION OF THE CONTENT OF A MULTICOMPONENT ADDITIVE ON BASED OIL COMPOSITION OF BIOLOGICALLY ACTIVE SUBSTANCES AND A PROBIOTIC

2020 ◽  
Author(s):  
М.В. ЛУКЬЯНЕНКО ◽  
Р.В. КАЗАРЯН ◽  
Т.В. ВАНИЦКАЯ ◽  
Е.В. КУЗЬМИНОВА ◽  
М.П. СЕМЕНЕНКО ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Feed Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Farm Animals ◽  
Feed Additive ◽  
Control Group ◽  
Oil Composition ◽  
Active Substances

Для интенсификации производства животноводческой продукции и повышения ее качества необходимо обогащать рацион сельскохозяйственных животных и птицы кормовыми добавками, содержащими комплекс биологически активных веществ. Исследованы образцы комплексной кормовой добавки, содержащей масляную композицию БАВ в-каротина, витаминов Е и С, жидкого рапсового лецитина и селена, и пробиотическую добавку (ПД) Целлобактерин в соотношениях соответственно от 1 : 1 до 1 : 5. Установлено, что при соотношении масляная композиция БАВ : ПД Целлобактерин 1 : 3 количество пробиотической культуры Enterococcus faecium 1-35, содержащейся в ПД Целлобактерин, в опытном образце достигает максимального значения 6,4 Ч 105 КОЕ/г. При этом обеспечивается равномерное распределение масляной композиции БАВ на поверхности ПД. В опытах in vivo определена эффективность применения комплексной добавки с установленным соотношением компонентов для нормализации обменных процессов в организме животных. Доказано, что введение в рацион лабораторных крыс опытной группы многокомпонентной кормовой добавки в количестве 3 к основному рациону (ОР), контаминированному микотоксинами, позволяет достичь опережения прироста массы тела на 15-е и 45-е сутки эксперимента на 6,45 и 33,05 соответственно снижения содержания мочевины в сыворотке крови на 58,43 по сравнению с аналогичными показателями у животных контрольной группы, получавших только ОР. Степень снижения аланинаминотрасферазы и аспартатаминотрансферазы по отношению к аналогичным показателям контрольной группы составила 18,53 и 29,89 соответственно. Следовательно, комплексная кормовая добавка при соотношении масляная композиция БАВ : ПД Целлобактерин 1 : 3 обладает ярко выраженными гепатопротекторными и антитоксическими свойствами. Enriching the diet of farm animals and poultry by feed additives containing a complex of biologically active substances is necessary to intensify the production of livestock products and improve their quality. Samples of a complex feed additive containing an oil composition of BAS в-carotene, vitamins E and C, liquid rapeseed lecithin and selenium, and a probiotic additive (PA) Cellobacterin in ratios from 1 : 1 to 1 : 5 respectively were studied. It was found that the ratio of the oil composition of BAS : PA Cellobacterin 1 : 3 respectively the amount of probiotic culture Enterococcus faecium 1-35 contained in PA Cellobacterin in the experimental sample reaches a maximum value 6,4 Ч 105 CFU/g. In this case, the distribution of BAS oil composition on the PA surface is uniform. The effectiveness of using a complex additive with a set ratio of components to normalize metabolic processes in animals was determined in in vivo experiments. It is established that introduction in a diet of laboratory rats of the experimental group multicomponent feed additive in an amount of 3 to the basic ration (BR), contaminated with mycotoxins, can achieve compared to the same indicators in animals of the control group who received only the BR, the timing of weight gain on 15-th and 45-th day of the experiment by 6,45 and 33,05 respectively reduction of urea content in blood serum at 58,43. The degree of reduction of alanine aminotransferase and aspartate aminotransferase in relation to similar indicators of the control group was 18,53 and 29,89 respectively. Therefore, a complex feed additive with the ratio of oil composition of BAS : PA Cellobacterin 1 : 3 has pronounced hepatoprotective and antitoxic properties.

Improving the fattening and meat traits of broiler chickens

2021 ◽  
pp. 38-51
Author(s):  
G. N. Vyayzenen ◽  
S. V. Razaev ◽  
A. G. Vyayzenen ◽  
N. S. Prodanov ◽  
A. E. Barashkov ◽  
...  
Keyword(s):  
Amino Acids ◽  
Laser Beam ◽  
Broiler Chickens ◽  
The Body ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Poultry Meat ◽  
Control Group ◽  
Active Substances

In order to save the cost of expensive compound feed, a number of biologically active substances (vitamins and amino acids) and improve the digestibility of nutrients for the first time in poultry meat using laser technology there is a real possibility of “transferring” the studied amino acids (methionine, glycine) and all water-soluble vitamins using a laser beam through the skin in the breast area of broiler chickens. The research has been carried out in Belgrankorm-Veliky Novgorod LLC in the Krestetsky district in the Novgorod region with a large-cage housing method of broiler chickens of the cross Hubbard. The duration of broilers rearing was 39 days, 4 recipes of compound feed based on wheat grain, corn, soybeen meal with the inclusion of fish flour, sunflower oil, corn gluten, sunflower meal, meat and bone meal and mineral additives (monocalcium phosphate, lime flour) have been used. For better assimilation of nutrients and biologically active substances by the body amino acids (methionine, glycine) and water-soluble vitamins Undevit, both individually and in combination with them in a ratio of 1:1 (by weight), in the form of powders passed through an infra-red laser beam in a spatial modulator, which were attached to two emitters (simultaneously) of the “Uzor-2K-Super” laser in the chest area have been used. Broilers of the experimental groups ate feed more often and faster than the herdmates of the control group. Air ozonation in the poultry house and broilers has been carried out in a mobile way using the Rios ozonator of the Decont series (mode 3, with a capacity of 20 g/m3 ). Innovative methods of influencing on the body caused the increase in the intensity of metabolic processes, growth and development, fattening and meat traits, and feed conversion in broilers.

scholarly journals THE INFLUENCE OF BIOLOGICALLY ACTIVE SUBSTANCES ON SHEEP RUMEN METABOLISM

2021 ◽  
pp. 35-41
Author(s):  
А.В. МИШУРОВ
Keyword(s):  
Rumen Fluid ◽  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Control Group ◽  
Potential Productivity ◽  
Organic Iodine ◽  
Rumen Metabolism ◽  
Experimental Group ◽  
Active Substances

Проблема и цель. Для повышения эффективности использования питательных веществ кормов целесообразно применение биологически активных веществ (БАВ), способствующих улучшению физиологических процессов в организме животных для реализации их генетического потенциала – продуктивности. Целью исследований являлось изучить влияние БАВ (дигидроквертицина и органического йода) на рубцовый метаболизм у овец. Методология. Опыт проведен методом групп периодов в условиях физиологического двора ФНЦ ВИЖ имени Л.К. Эрнста, на овцах аналогах (n=6), имеющих хронические фистулы рубца по Басову. Животные 1-й опытной группы в дополнение к основному рациону получали 100 мл ДКВ, в рацион 2-й опытной группы добавляли 100 мг ДКВ и 1,05 мг органического йода. Результаты. Использование добавок в рационах животных опытных групп повлияло на общее содержание микроорганизмов в рубцовой жидкости, составившее во второй опытной группе 1,021 г/100 мл, что выше чем в первой опытной группе на 12,7 %, и выше, чем в контрольной, на 15,4 %. При этом образование бактерий в рубцовой жидкости в первой опытной группе было выше на 22,5 %, а во второй – на 8,7 % относительно контроля. Выявлен более низкий уровень концентрации аммиака в содержимом рубца животных первой опытной группы на 4,2 мг/% относительно контрольной и на 7,1 мг/% – второй опытной группы, что взаимосвязано с более эффективным его использованием рубцовой микрофлорой. Заключение. Полученные данные свидетельствуют о том, что использование в рационе изучаемых биологически активных веществ способствовало наибольшему развитию и жизнедеятельности микроорганизмов рубца и в целом способствовало регуляции положительной динамики рубцового метаболизма, оказало положительное влияние на образование и усвояемость азота. Problem and purpose. To increase the efficiency of the use of feed nutrients, it is advisable to use biologically active substances that contribute to the improvement of physiological processes in the body of animals for the realization of their genetic potential - productivity. The aim of the research was to study the effect of biologically active substances (dihydroquerticin and organic iodine) on rumen metabolism in sheep. Methodology. The experiment was carried out by the method of groups of periods in the conditions of the physiological yard of L.K. Ernst Federal Research Center for Animal Husbandry), on sheep analogs (n=6) with chronic rumen fistulas according to Basov. Animals of the 1st experimental group received 100 ml of DHQ in addition to the main diet. The 2nd experimental group got 100 mg of DHQ and 1.05 mg of organic iodine. Results. The use of additives in the diets of the animals of the experimental groups influenced the total content of microorganisms in the rumen fluid, which was 1.021 g / 100 ml in the second experimental group, that was higher than in the first experimental group by 12.7 %, and in the control group by 15.4 %. At the same time, the formation of bacteria in the rumen fluid in the 1st experimental group was 22.5 % higher and it was 8.7 % higher in the 2nd group relative to the control. A lower level of ammonia concentration in the rumen of animals of the 1st experimental group was 4.2 mg/% lower relative to the control and by 7.1 mg/% in the 2nd experimental group, that was interrelated with its more effective use of rumen microflora. Conclusion. The data obtained indicated that the use of the studied biologically active substances in the diet promoted the greatest development and vital activity of rumen microorganisms and, in general, contributed to the regulation of the positive dynamics of rumen metabolism, had a positive effect on the formation and assimilation of nitrogen.

scholarly journals INFLUENCE OF BIOLOGICALLY ACTIVE SUBSTANCES OF PROPOLIS ON BIOCHEMICAL INDICATORS OF BLOOD AND MORPHOMETRIC INDICATORS OF INTESTINE OF YOUNG PIGS

2021 ◽  
Vol 2021 (1) ◽  
pp. 55-64
Author(s):  
Nikolay Gaponov
Keyword(s):  
Experimental Studies ◽  
Biological Properties ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Intestinal Cell ◽  
Control Group ◽  
Medicine Research ◽  
Young Pigs ◽  
Experimental Group ◽  
Active Substances

This paper presents the results of experimental studies the influence of biologically active substances of propolis on micromorphometric parameters of the ileum in young pigs. The results of the experiment make it possible to ascertain a change in the functional activity of the studied organ after the use of the drug from propolis. Since the most active process of absorption of monomeric hydrolysis products, especially amino acids and carboxylic acids, occurs in the ileum. Hydrolysis of intermediate products to monomers and preparation of the transition to absorption is carried out by enzymes fixed on the intestinal cell membranes. And the level of bioconversion of feed and retention of nutrients depends on their shape and structure, which ultimately affects the payment for feed with products. Therefore, the study of the histology of the small intestine and especially the ileum is of great importance. So, as a result of the study, it was found that the most characteristic histological changes in the structural components of the intestinal wall are observed in young animals of the experimental groups. The thickness of the main lamina of the mucous membrane of the ileum in piglets in the control group is 308.28 ± 2.81 µm, in piglets in the experimental group – 255.49 ± 0.75 µm. The height of the villi of the ileum, in the animals of the control group, is significantly lower and amounts to 193.89 ± 2.66 µm versus 280.97 ± 3.37 µm in the experimental group. Similar changes are observed in young animals of the older age group. The height of intestinal villi in pigs of the experimental group significantly exceeds the indicator of the control group and is 256.89 ± 1.62 µm and 163.96 ± 0.61 µm, respectively. The muscle plate of the intestinal mucosa of the pigs in the experimental group is significantly thicker than the control and is 17.8 ± 0.2 μm and 8.89 ± 0.08 μm, respectively. The obtained experimental material substantially supplements the information on the biological properties of propolis preparations. In practical veterinary medicine, research results can be taken into account when prescribing propolis for medicinal and prophylactic purposes. Since histological analysis of intestinal sections did not reveal destructive or pathological changes in any of the compared groups. In feeding, using the obtained practical material, we can specify the stimulating effect of the propolis preparation to intensify the growth of animals depending on the age of the pigs.

scholarly journals PHARMACOLOGICAL STUDY OF OINTMENT BASED ON ZINGIBER OFFICINALE EXTRACT

2021 ◽  
Author(s):  
Lidiia Eberle ◽  
◽  
Alona Kobernik ◽  
Keyword(s):  
Medicinal Plants ◽  
Diclofenac Sodium ◽  
Zingiber Officinale ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Control Group ◽  
Ginger Extract ◽  
Inflammatory Reactions ◽  
Anti Inflammatory ◽  
Active Substances

Nonsteroidal anti-inflammatory drugs such as ibuprofen and diclofenac sodium are widely known drugs for the treatment of inflammatory reactions. Prolonged use of drugs inevitably leads to the development of adverse reactions and reduced immunity of patients. Since the reduction of the "drug load" is an urgent task in this situation, an alternative direction to solve it is the use of natural therapeutic factors, the rehabilitation capabilities of which in these conditions are very appropriate and effective. There is a whole arsenal of medicinal plants in the world, they contain a complex of biologically active substances and are not inferior in therapeutic properties to synthetic agents. Among such medicinal plants is Zingiber officinale, which for the past 10 years is actively grown in Ukraine in the city of Kherson. The study shows the relevance of making a mild drug based on a thick extract of the rhizome of Zingiber officinale. It is proved that the extract belongs to low-toxic compounds, which allows its use in the composition of drugs for transdermal administration. An ointment was made on the basis of a thick ginger extract with a content of biologically active substances of 0.025%. The content was standardized according to the content of the sum of polyphenolic compounds. Inflammation was induced by subplantar injection to the plantar fasciitis (aponeurosis) of the hind limb of rats using 30 µL AITC solution (100 µg/limb) in 1,2-propyleneglycol. The dynamics of changes of inflammatory process was evaluated before addition of the inflammation inducer and after 1, 2, 3, 4, 6 and 24 hours of its injection for measuring the volume and the thickness of affected limb. Ointment based on ginger extract has a high level of anti-inflammatory properties, which is shown on the basis of the positive dynamics of changes in the morphological parameters of the affected limbs of animals. Prophylactic application of the phytopreparation two days before the start of the experiment helped to block the development of inflammation in comparison with the control group and the reference drug – ibuprofen ointment. The soft dosage form created and studied by us based on the thick extract of Zingiber officinale is an effective anti-inflammatory agent that can not only treat inflammation, but also block its development under previous prophylactic applications.

scholarly journals Neutrophils stimulation index in people under consumption of broiler chickens meat at pre-slaughter stress correction

2015 ◽  
Vol 6 (2) ◽  
pp. 165-168
Author(s):  
S. Grabovskyi ◽  
O. Grabovska
Keyword(s):  
Broiler Chickens ◽  
Broiler Chicken ◽  
Specific Resistance ◽  
Stimulation Index ◽  
Chicken Meat ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Control Group ◽  
Active Substances ◽  
Chicken Blood

The data about changes in neutrophils stimulation index in men blood after consumption of broiler chicken meat with the natural origin immunomodulators, introduced in feed before slaughter, is presented in this paper. Spleen extract biologically active substances were used as immunomodulators and anti-stressors during pre-slaughter period. Biologically active substances influence on putrescin, spermine and spermidine content in broiler chicken blood before slaughter and on some non-specific resistance indices in people was determined after consumption of broiler chicken meat. Two groups of broiler chickens at one month age were formed for the study. The spleen extract obtained with ultrasound application (I research group) served as biologically active substances was added to the feed of broiler chickens in pre-slaughter period (five days before slaughter). Blood polyamines such as putrescin, spermine and spermidine were determined by the method of High-performance liquid chromatography (HPLC) on the liquid chromatograph Agilent 1200 (USA). The second experiment was conducted on 10 people. We recruited 10 healthy male medical students (20 years old, on average) after the National Medical license examination. Spleen extract polyamines as immunomodulators and anti-stressors have the most effective influence on total quantity of polyamines in broiler chicken blood. As a result of research, it is found that aerosol introduction of spleen extract into broiler chicken feed reliably increases total quantity of polyamines by 39% and, in particular, spermidine concentration by 34%, and spermine by 40% compared with broiler chickens of the control group. Some non-specific body resistance indices in men blood upon consumption of broiler chicken meat varied within the physiological norm. The neutrophils stimulation index increased in men blood (+0,82) after consumption of meat of broiler chickens to which spleen extract as immunomodulator and anti-stressor was introduced before slaughter (experimental group). The neutrophils stimulation index decreased in men blood (–2,21) after consumption of broiler chickens meat at pre-slaughter stress (control group). The neutrophils stimulation index in men blood of experimental and control groups differed by 34,8%, but did not go beyond the physiological norm. At the final stage of poultry feeding it is necessary to consider pre-slaughter stress and to apply biologically active substances of natural origin, such as spleen extract. The results obtained in the experiment on broiler chickens can be used in studies of non-specific resistance indices of the farm animals for increasing the organism resistance, correction and avoiding of pre-slaughter stress and improvement of product quality

scholarly journals ГОРМОНАЛЬНИЙ ПРОФІЛЬ ТА НЕСПЕЦИФІЧНА РЕЗИСТЕНТНІСТЬ КНУРІВ ЗА УМОВ ПЕРЕДЗАБІЙНОГО СТРЕСУ

2016 ◽  
Vol 6 (01) ◽  
pp. 80-93
Author(s):  
S.S. Grabovskyi ◽  
O.S. Grabovska
Keyword(s):  
Blood Plasma ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Animal Origin ◽  
Control Group ◽  
Control Groups ◽  
Natural Origin ◽  
And Control ◽  
Active Substances

<p>The article is devoted to determination of hormonal profile and nonspecific resistance in boars blood before slaughter after using of biologically active substances — animal origin antistressors andimmunostimulators.</p><p><strong>The purpose</strong><strong> </strong><strong>of research</strong> — determination of changes of insulin, adrenocorticotropic hormone (ACTH), cortisol content in boars blood before slaughter and their correction of natural origin biologically active substances of spleen extract.</p><p><strong>Object and research methods</strong>. The spleen extract has been additionally entered to the boars feed at 5 days before slaughter as an antistressors and immunostimulators in pre-slaughter period. The experiment was conducted on 15 boars with standard diet. Three groups of boars six months of age (5 boars each) were formed for research. The pig’s spleen extract was obtained with ultrasound application (Iresearch group) were using as a biologically active substances to the feed boars in pre-slaughter period. The extracts were applied to dry feed by aerosol method (70 % alcohol solution of spleen extract volume of 1.4 ml per kg body weight). The boars of II research group in the same way received to the feed of 70 % ethanol solution in the same volume. The boars of control group received only dry feed economy. Theboars slaughter was held on day 13 hours a.m. Mathematical treatment of the research results worked statistically using the software package Statistica 6.0.</p><p><strong>Results and discussion</strong>. The ACTH and cortisol level in the boars’ blood plasma of experimental and control groups significantly increased after transportation (before the slaughter) compared with the indexes before transportation to meat plant. The ACTH concentration in the boars’ blood plasma of І experimental group, which was added to the basic diet spleen extract, was 10 % lower than in the control group boars compared with indicators before and after transportation. The insulin concentration of І experimental and control groups boars was higher after transportation with comparing beforetransportation to meat plant.</p><p>All nonspecific body resistance parameters significantly increased in boars blood, which in addition to food received spleen extract, compared with the control group animals after transportation before slaughter: phagocytic activity increased at 12 %, phagocytic index — 13 % and phagocytic number — 14 %.</p><p>On the boars’ feeding final stage is necessary to consider a pre-slaughter stress and to apply of natural origin biologically active substances. The results can be used on other farm animals for resistance increasing, correction and avoid their pre-slaughter stress and improve the product quality.</p>

Microscopic fungi of lithobiont communities of Argentine Islands Region: Data from the 22nd Ukrainian Antarctic Expedition

2020 ◽  
Vol 62 (1-2) ◽  
pp. 49-68
Author(s):  
T. O. Kondratiuk ◽  
T. V. Beregova ◽  
I. Yu. Parnikoza ◽  
S. Y. Kondratyuk ◽  
A. Thell
Keyword(s):  
Filamentous Fungi ◽  
Mucor Circinelloides ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Microscopic Fungi ◽  
Rhodotorula Rubra ◽  
Yeast Fungi ◽  
Antarctic Expedition ◽  
Geomyces Pannorum ◽  
Active Substances

The identification of the diversity of microscopic fungi of lithobiont communities of the Argentine Islands in specimens collected during the 22nd Ukrainian Antarctic Expedition was the purpose of this work. Samples of rock, soil, mosses and lichens of rock micro-habitats of “Crustose lichen sub-formation and fruticose lichen and moss cushion sub-formation” were used in the work. These samples were used for extracting and cultivation of filamentous fungi on dense nutrient media. Determination of physiological and biochemical characteristics and identification of yeast-like fungi were performed using a microbiological analyser ‘Vitek-2’ (‘Bio Merieux’, France). Cultivation of microorganisms was carried out at temperatures from +2 to +37 °C. In results cultures of microscopic fungi of Zygomycota (Mucor circinelloides), Ascomycota (species of the genera cf. Tlielebolus, Talaromyces), representatives of the Anamorphic fungi group (Geomyces pannorum, species of the genera Alternaria, Acremonium, Aspergillus, Penicillium, and Cladosporium) were isolated from Antarctic samples. Microscopic fungi Penicillium spp. were dominated after the frequency in the studied samples (54.5%). Rhodotorula rubra and Candida sp. among isolated yeast fungi, and dark pigmented fungi represented by Aureobasidium pulhdans and Exophiala spp. were identified. The biological properties of a number of isolated fungi (the potential ability to synthesise important biologically active substances: melanins, carotenoids, lipids) are characterised. Mycobiota of rock communities of Argentine Islands is rich on filamentous and yeast fungi similarly to other regions of Antarctica. A number of fungi investigated are potentially able to synthesise biologically active substances. The dark pigmented species of the genera Cladosporium, Exophiala, Aureobasidium pulhdans, capable of melanin synthesis; ‘red’ yeast Rhodotorula rubra (carotenoid producers and resistant to toxic metals); Mucor circinelloides and Geomyces pannorum, lipid producers, are among these fungi. Yeast-like fungi assimilated a wide range of carbohydrates, which will allow them to be further used for cultivation in laboratory and process conditions. The collection of technologically promising strains of microorganisms, part of the Culture Collection of Fungi at Taras Shevchenko National University of Kyiv (Ukraine), is updated with isolated species (strains) of filamentous fungi and yeast – potential producers of biologically active substances, obtained within this study.

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