Microbiota in intra-oral halitosis – characteristics, effects of antibacterial mouth rinse treatment

2020 ◽  
Vol 25 (1) ◽  
pp. 4-9
Author(s):  
D. S. Vikina ◽  
I. N. Antonova ◽  
V. V. Tec ◽  
T. E. Lazareva
Keyword(s):  
Antimicrobial Agents ◽  
Oral Microbiota ◽  
Mouth Rinse ◽  
Oral Malodor ◽  
Microbial Biofilms ◽  
Mouth Rinses ◽  
Oral Malodour ◽  
Granulicatella Adiacens ◽  
Bad Breath ◽  
Spore Forming Bacteria

Relevance. Modern data confirm multimicrobial etiology of oral malodor that can significantly compromise effective therapy. Thus, it is relevent to perform comprehensive study of causes of oral malodour, based on microbiological study of composition of mixed microbial biofilms including culturable and non-culturable microbes and evaluation of efficacy of mouth rinses with broad-spectrum antimicrobial agents.Purpose. To study oral microbiota in oral malodor and its correction with antibacterial mouth rinses.Materials and methods. Subjects, involved in the study, were divided into 3 groups, each having 20 people. Controls didn’t use mouth rinses; experimental group used mouth rinse, containing multicidum; in comparison group chlorhxidine containing mouth rinse was used. Orgnoleptic measurement based on 0-5 Rosenberg scale was applied to score intensity of oral malodor. Coating of the root of the tongue was studied by culture-based methods.Results. The research showed that Multicidum® mouth rinse in comprehensive oral malodor treatment affects aerobic bacteria involved in the pathologic process causing bad breath. Positive effect manifested itself organoleptically according to Rosenberg scale in foul breath reduction and in composition changes of microbiota of the root of the tongue. At thus, amount of microorganisms producing smelly volatile compounds decreased and the number of bacteria inhibiting this process increased amid reduction of spore-forming bacteria that contribute to maintenance of stable microbial communities in oral malodor.Conclusion. Gram-positive cocci and Bacillus-Streptococcus, Enterobacter, Staphylococcus, Granulicatella adiacens, Rothia and aerobic spore-forming bacteria from the Bacillus family that are involved in maintaining the functions of resistant bacterial communities-predominated among the bacteria identified in patients with halitosis. There was a statistically significant decrease in the severity of halitosis under the action of a rinse aid, the main active substance of which is Multicidum , and the absence of a statistically significant effect when using a rinse aid based on chlorhexidine.

scholarly journals Antimicrobial Susceptibilities of Oral Isolates of Abiotrophia and Granulicatella According to the Consensus Guidelines for Fastidious Bacteria

Medicines ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 129 ◽  
Author(s):  
Taisei Kanamoto ◽  
Shigemi Terakubo ◽  
Hideki Nakashima
Keyword(s):  
Dna Hybridization ◽  
Vitamin B6 ◽  
Antimicrobial Agents ◽  
Oral Microbiota ◽  
Phenotypic Characteristics ◽  
Granulicatella Adiacens ◽  
Susceptibility Profiles ◽  
Susceptibility Rate ◽  
Type Strains ◽  
Culture Negative

Background: The genera Abiotrophia and Granulicatella, previously known as nutritionally variant streptococci (NVS), are fastidious bacteria requiring vitamin B6 analogs for growth. They are members of human normal oral microbiota, and are supposed to be one of the important pathogens for so-called “culture-negative” endocarditis. Methods: The type strains and oral isolates identified, by using both phenotypic profiles and the DNA–DNA hybridization method, were examined for susceptibilities to 15 antimicrobial agents including penicillin (benzylpenicillin, ampicillin, amoxicillin, and piperacillin), cephem (cefazolin, ceftazidime, ceftriaxone, and cefaclor), carbapenem (imipenem), aminoglycoside (gentamicin), macrolide (erythromycin), quinolone (ciprofloxacin), tetracycline (minocycline), glycopeptide (vancomycin), and trimethoprim-sulfamethoxazole complex. The minimum inhibitory concentration and susceptibility criterion were determined, according to the consensus guideline from the Clinical and Laboratory Standards Institute. Results: Isolates of Abiotrophia defectiva were susceptible to ampicillin, amoxicillin ceftriaxone, cefaclor, imipenem, ciprofloxacin, and vancomycin. Isolates of Granulicatella adiacens were mostly susceptible to benzylpenicillin, ampicillin, amoxicillin, cefazolin, ceftriaxone, imipenem, minocycline, and vancomycin. The susceptibility profile of Granulicatella elegans was similar to that of G. adiacens, and the susceptibility rate was higher than that of G. adiacens. Conclusions: Although Abiotrophia and Granulicatella strains are hardly distinguishable by their phenotypic characteristics, their susceptibility profiles to the antimicrobial agents were different among the species. Species-related differences in susceptibility of antibiotics should be considered in the clinical treatment for NVS related infections.

scholarly journals Halitosis: a serious social problem

1970 ◽  
Vol 9 (2) ◽  
pp. 106-111 ◽  
Author(s):  
S Sharma ◽  
TP Shankar ◽  
B Koirala
Keyword(s):  
Metabolic Diseases ◽  
Social Stigma ◽  
Systemic Disease ◽  
The United States ◽  
Economic Consequences ◽  
Tongue Coating ◽  
Oral Malodor ◽  
Oral Malodour ◽  
Unpleasant Odor ◽  
Bad Breath

Halitosis means an unpleasant odor of the expired air, regardless of the source of this odor. The foul breath originating from the oral cavity itself is termed as oral malodor and it is usually the main contributor of unpleasant breath in an otherwise healthy individual. The most predominant causes of halitosis are gingivitis, periodontitis, and tongue coating. However, several non-oral pathologic conditions have also been related to halitosis, including infection of the upper and lower respiratory tracts, the gastrointestinal tract, and some metabolic diseases involving the kidneys and the liver, which should never be overlooked. Though this complaint of bad breath has existed from time immemorial, it has only recently come to scientific platforms of analysis and is being increasingly recognized as a social stigma in an ever-growing sensitive society. Breath malodor has important socio-economic consequences. Literature reveals almost $1 billion a year is spent in the United States on deodorant-type mouth rinses and mints which provide only short-term masking effects, instead of spending the money on a proper diagnosis and etiologic care to manage bad breath. Sulfur containing volatiles are the main constituent of oral malodor and that their levels correlate with the intensity as determined organoleptically. The treatment of intraoral halitosis consists of scaling/root planing and daily removal of tongue coating, and for extraoral halitosis, treatment of underlying systemic disease by a specialist. Keywords: halitosis; bad breath; oral malodour DOI: http://dx.doi.org/10.3126/hren.v9i2.4983 Health Renaissance 2011: Vol.9 (No.2): 106-111

scholarly journals Differential Effects of Antiseptic Mouth Rinses on SARS-CoV-2 Infectivity In Vitro

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 272
Author(s):  
Chuan Xu ◽  
Annie Wang ◽  
Eileen R. Hoskin ◽  
Carla Cugini ◽  
Kenneth Markowitz ◽  
...  
Keyword(s):  
Potential Benefit ◽  
Povidone Iodine ◽  
Cellular Damage ◽  
Virus Infectivity ◽  
Viral Infectivity ◽  
Mouth Rinse ◽  
Prolonged Incubation ◽  
Differential Effects ◽  
Mouth Rinses

Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) is detectable in saliva from asymptomatic individuals, suggesting a potential benefit from the use of mouth rinses to suppress viral load and reduce virus spread. Published studies on the reduction of SARS-CoV-2-induced cytotoxic effects by mouth rinses do not exclude antiseptic mouth rinse-associated cytotoxicity. Here, we determined the effect of commercially available mouth rinses and antiseptic povidone-iodine on the infectivity of replication-competent SARS-CoV-2 viruses and of pseudotyped SARS-CoV-2 viruses. We first determined the effect of mouth rinses on cell viability to ensure that antiviral activity was not a consequence of mouth rinse-induced cytotoxicity. Colgate Peroxyl (hydrogen peroxide) exhibited the most cytotoxicity, followed by povidone-iodine, chlorhexidine gluconate (CHG), and Listerine (essential oils and alcohol). The potent antiviral activities of Colgate Peroxyl mouth rinse and povidone-iodine were the consequence of rinse-mediated cellular damage when the products were present during infection. The potency of CHG was greater when the product was not washed off after virus attachment, suggesting that the prolonged effect of mouth rinses on cells impacts the antiviral outcome. To minimalize mouth rinse-associated cytotoxicity, mouth rinse was largely removed from treated viruses by centrifugation prior to infection of cells. A 5% (v/v) dilution of Colgate Peroxyl or povidone-iodine completely blocked viral infectivity. A similar 5% (v/v) dilution of Listerine or CHG had a moderate suppressive effect on the virus, but a 50% (v/v) dilution of Listerine or CHG blocked viral infectivity completely. Mouth rinses inactivated the virus without prolonged incubation. The new infectivity assay, with limited impacts of mouth rinse-associated cytotoxicity, showed the differential effects of mouth rinses on SARS-CoV-2 infection. Our results indicate that mouth rinses can significantly reduce virus infectivity, suggesting a potential benefit for reducing SARS-CoV-2 spread.

Decreasing Cariogenic Bacteria with a Natural, Alternative Prevention Therapy utilizing Phytochemistry (Plant Extracts)

2011 ◽  
Vol 36 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Y Ramakrishna ◽  
H Goda ◽  
MS Baliga ◽  
AK Munshi
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Periodontal Diseases ◽  
Treatment Options ◽  
Biologically Active ◽  
Oral Microbiota ◽  
Toxic Exposure ◽  
Oral Diseases ◽  
Cariogenic Bacteria ◽  
Traditional Medicines

The association between the oral microbiota and oral diseases is well established. Various antimicrobial agents including antibiotics are commercially available against oral pathogenic bacteria. For the reasons of antibiotic resistance, their adverse effects and financial considerations in the developing countries, there is a need for alternate preventive and curative treatment options that are also safe, effective and economical. Traditional medicines have been used since ancient times for the treatment of oral diseases including dental caries, periodontal diseases that affect the majority of the population and can affect a person's overall health. Natural phytochemicals are certain organic components isolated from plants and some of these extracts are considered to be beneficial to health. They serve as antioxidants, enhance immune response,provide protection against oral cancer and other diseases and also repair DNA damage caused by smoking and other toxic exposure, and detoxify carcinogens. The natural products derived from medicinal plants have proven to be an abundant source of biologically active compounds, many of which have been the basis for the development of new lead chemicals for pharmaceuticals.They are considered to be good alternatives to synthetic chemicals. This article presents a review of natural alternatives derived from plants and plant products that can serve as a prevention and treatment option against cariogenic bacteria.

scholarly journals 330 The use of lactoferrin and lactoperoxidase for oral health

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 67-67
Author(s):  
Manabu Nakano ◽  
Miyuki Tanaka ◽  
Fumiaki Abe
Keyword(s):  
Placebo Group ◽  
Oral Health ◽  
Test Group ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Elderly Subjects ◽  
Oral Microbiota ◽  
Oral Malodor ◽  
Related Quality ◽  
Health Related

Abstract Lactoferrin (LF) and lactoperoxidase (LPO) are known as host defense factors in milk and saliva. LF has antibacterial and biofilm-inhibitory effects against periodontopathic bacteria. LPO catalyzes the formation of hypothiocyanite and exhibits bactericidal activity within a short time. Our research group developed LF- and LPO-containing powders, and examined their applications for oral hygiene. In this presentation, we review the clinical efficacies of LF- and LPO-containing tablets on oral malodor and oral microbiota in humans. We performed a randomized clinical trial to assess the effects of the single ingestion of LF- and LPO-containing tablets on malodor. The main components causing oral malodor are volatile sulfur compounds (VSCs). Subjects with oral malodor ingested a test or placebo tablet in two crossover phases. The concentrations of total VSCs at 10 min after ingestion were significantly lower in the test group than in the placebo group. The effects of LF- and LPO-containing tablets on oral microbiota were also investigated. Elderly subjects ingested test or placebo tablets for 8 weeks. The test group exhibited a significant reduction in Porphyromonas gingivalis compared with the placebo group. Microbiota analysis using next-generation sequencing of the bacterial 16S rRNA genes revealed that the LF- and LPO-containing tablets suppressed the relative abundance of periodontal bacteria, but not that of indigenous bacteria such as Streptococcus. Another trial suggested that the regular intake of LF- and LPO-containing tablets can improve gingival inflammation and oral health-related quality of life in healthy adults. Our findings suggest the potential of LF- and LPO-containing tablets as a daily treatment to maintain good oral health and prevent progression to periodontitis. We consider these effects of LF and LPO to also be applicable to pet food for oral health.

scholarly journals Efficacy of Experimental Mouth Rinses on Caries-Related Biofilms in vitro

2021 ◽  
Vol 2 ◽  
Author(s):  
Josiana Steiger ◽  
Olivier Braissant ◽  
Tuomas Waltimo ◽  
Monika Astasov-Frauenhoffer
Keyword(s):  
Growth Rate ◽  
Antimicrobial Agents ◽  
Growth Parameters ◽  
Screening Method ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Screening Process ◽  
Mouth Rinses ◽  
Amine Fluoride

This study assessed the efficacy of tin and Polyethylenglycol (PEG-3) tallow aminopropylamine in different concentrations on Streptococcus mutans (S. mutans) biofilms to establish a new screening process for different antimicrobial agents and to gain more information on the antibacterial effects of these agents on cariogenic biofilms. Isothermal microcalorimetry (IMC) was used to determine differences in two growth parameters: lag time and growth rate; additionally, reduction in active biofilms was calculated. Experimental mouth rinses with 400 and 800 ppm tin derived from stannous fluoride (SnF2) revealed results (43.4 and 49.9% active biofilm reduction, respectively) similar to meridol mouth rinse (400 ppm tin combined with 1,567 ppm PEG-3 tallow aminopropylamine; 55.3% active biofilm reduction) (p > 0.05), while no growth of S. mutans biofilms was detected during 72 h for samples treated with an experimental rinse containing 1,600 ppm tin (100% active biofilm reduction). Only the highest concentration (12,536 ppm) of rinses containing PEG-3 tallow aminopropylamine derived from amine fluoride (AmF) revealed comparable results to meridol (57.5% reduction in active biofilm). Lower concentrations of PEG-3 tallow aminopropylamine showed reductions of 16.9% for 3,134 ppm and 33.5% for 6,268 ppm. Maximum growth rate was significantly lower for all the samples containing SnF2 than for the samples containing control biofilms (p < 0.05); no differences were found between the control and all the PEG-3 tallow aminopropylamine (p > 0.05). The growth parameters showed high reproducibility rates within the treated groups of biofilms and for the controls; thus, the screening method provided reliable results.

scholarly journals Carbohydrate Mouth Rinse Mitigates Mental Fatigue Effects on Maximal Incremental Test Performance, but Not in Cortical Alterations

2020 ◽  
Vol 10 (8) ◽  
pp. 493 ◽  
Author(s):  
Cayque Brietzke ◽  
Paulo Estevão Franco-Alvarenga ◽  
Raul Canestri ◽  
Márcio Fagundes Goethel ◽  
Ítalo Vínicius ◽  
...  
Keyword(s):  
Motor Cortex ◽  
Exercise Performance ◽  
Test Performance ◽  
Time Trial ◽  
Mental Fatigue ◽  
Mouth Rinse ◽  
Incremental Test ◽  
Mouth Rinses ◽  
Spontaneous Behavior

Detrimental mental fatigue effects on exercise performance have been documented in constant workload and time trial exercises, but effects on a maximal incremental test (MIT) remain poorly investigated. Mental fatigue-reduced exercise performance is related to an increased effort sensation, likely due to a reduced prefrontal cortex (PFC) activation and inhibited spontaneous behavior. Interestingly, only a few studies verified if centrally active compounds may mitigate such effects. For example, carbohydrate (CHO) mouth rinse potentiates exercise performance and reduces effort sensation, likely through its effects on PFC activation. However, it is unknown if this centrally mediated effect of CHO mouth rinse may mitigate mental fatigue-reduced exercise performance. After a proof-of-principle study, showing a mental fatigue-reduced MIT performance, we observed that CHO mouth rinse mitigated MIT performance reductions in mentally fatigued cyclists, regardless of PFC alterations. When compared to placebo, mentally fatigued cyclists improved MIT performance by 2.24–2.33% when rinsing their mouth with CHO during MIT. However, PFC and motor cortex activation during MIT in both CHO and placebo mouth rinses were greater than in mental fatigue. Results showed that CHO mouth rinse mitigated the mental fatigue-reduced MIT performance, but challenged the role of CHO mouth rinse on PFC and motor cortex activation.

scholarly journals A Systematic Review of the Effect of Oral Rinsing with H2O2 on Clinical and Microbiological Parameters Related to Plaque, Gingivitis, and Microbes

2020 ◽  
Vol 2020 ◽  
pp. 1-18
Author(s):  
Francisco Wilker Mustafa Gomes Muniz ◽  
Juliano Cavagni ◽  
Gerson Pedro José Langa ◽  
Bernal Stewart ◽  
Zilson Malheiros ◽  
...  
Keyword(s):  
Systematic Review ◽  
Hydrogen Peroxide ◽  
Dental Plaque ◽  
Oral Bacteria ◽  
Cochrane Library ◽  
Oral Microbiota ◽  
Supporting Evidence ◽  
Gingival Inflammation ◽  
Mouth Rinses ◽  
Microbiological Parameters

Background. Hydrogen peroxide (H2O2) has been used for more than a century clinically to control plaque and gingival inflammation, with unclear supporting evidence. Aim. The aim of the present systematic review of the literature is to assess the effect of mouth rinses with H2O2 on dental plaque, gingival inflammation, and oral microorganisms. Methods. Five databases (PubMed, Scopus, Embase, Cochrane Library, and Web of Science) were searched with the following focused question: what is the effect of hydrogen peroxide, in comparison to chlorhexidine or to a placebo solution, in oral microbiota control, dental plaque, and gingival inflammatory outcomes? Two independent examiners retrieved the articles and evaluated the evidence. Results. The majority of included studies were performed with 1.5% H2O2. Results related to plaque accumulation generally demonstrate a slightly better effect of H2O2 as compared to placebo mouth rinses, however with a lower performance as compared to chlorhexidine. In terms of gingival inflammation, H2O2 performs better than placebo and more clearly demonstrates an anti-inflammation effect. No studies evaluated the effect of H2O2 against viruses or fungi. In terms of bacteria, H2O2 demonstrates an antibacterial effect. Conclusion. Rinsing with H2O2 has the potential to affect plaque, gingivitis, and oral bacteria, as compared to placebo. However, the antibacterial results are not comparable to the performance of chlorhexidine.

scholarly journals Comparative Evaluation of Efficacy of Chlorhexidine and Herbal Mouthwash as a Preprocedural Rinse in Reducing Dental Aerosols: A Microbiological Study

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Sangeeta Umesh Nayak ◽  
Anushka Kumari ◽  
Valliammai Rajendran ◽  
Vijendra Pal Singh ◽  
Ashwini Hegde ◽  
...  
Keyword(s):  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Study Patient ◽  
Microbial Culture ◽  
Mouth Rinse ◽  
Mouth Rinses ◽  
Intergroup Comparison ◽  
Viable Bacteria ◽  
Post Hoc

Objective. The risk to dentists, assistants, and patients of infectious diseases through aerosols has long been recognized. The aim of this study was to evaluate and compare the efficacy of commercially available preprocedural mouth rinses containing 0.2% chlorhexidine (CHX) gluconate, Befresh™ herbal mouthwash, and water in reducing the levels of viable bacteria in aerosols. Materials & Methods. This was a single-center, double-masked, placebo-controlled, randomized, three-group parallel design study. 30 patients (10 patients in each group) were recruited in the study. Patient rinsed mouth with 10 ml of CHX, 10 ml Befresh™, or 10 ml water. All the patients underwent supragingival ultrasonic scaling for a minimum of 30 min. The aerosol collection was done using a blood agar plate. The blood agar plates were kept approximately 12 inches from the patient’s mouth. The microbial culture was analyzed. The colony-forming unit (CFU) counting in all three groups was assessed using one-way ANOVA test to compare among the groups (p value <0.001). The intergroup comparison was done using the post hoc Tukey test. Result. There was a marked reduction in the CFU in the CHX group in all three areas. This was followed by Befresh™ (Sagar Pharmaceuticals) mouthwash. There was no reduction in the CFU of the water group. Conclusion. This study proves that a regular preprocedural mouth rinse could significantly reduce the majority bacteria present in aerosols generated by the use of an ultrasonic unit, and Befresh™ mouth rinse was found to be equally effective in reducing the aerosol contamination to 0.2% CHX gluconate.

scholarly journals Does a pretreatment with a dentine hypersensitivity mouth-rinse compensate the pain caused by professional mechanical plaque removal? A single-blind randomized controlled clinical trial

2020 ◽  
Author(s):  
Sonja H. M. Derman ◽  
Eva-Maria Lantwin ◽  
Anna Greta Barbe ◽  
Michael J. Noack
Keyword(s):  
Rating Scale ◽  
Periodontal Therapy ◽  
Point Of View ◽  
Controlled Clinical Trial ◽  
Mouth Rinse ◽  
Potassium Oxalate ◽  
Plaque Removal ◽  
Mouth Rinses ◽  
Supportive Periodontal Therapy ◽  
Dentinal Hypersensitivity

Abstract Objectives Pain is affecting acceptance of supportive periodontal therapy and primary periodontitis prevention. Our objective was to evaluate the efficacy of a 1-week pre-treatment use of dentinal-hypersensitivity-reducing mouth-rinses (DHM) in periodontal maintenance (SPT) or dental prophylaxis patients. Material and methods One hundred fifty-five participants attending for professional mechanical plaque removal (PMPR) were randomly assigned to use a mouth-rinse twice daily for 1 week prior to their next PMPR. Rinses were containing either potassium oxalate (n = 52), arginine (n = 52), or herbal extracts (n = 51). At baseline and reassessment, procedural pain was assessed by visual analogue scale (VAS) and verbal rating scale (VRS). Self-reported efficacy was documented. Results No inter-group differences were estimated between both test groups and the control for baseline and reassessment means (VAS, VRS). In the SPT group, VAS reduction and self-reported efficacy were found (p < 0.05). Conclusion The 1-week use of DHM failed to show a predictable effect on discomfort during PMPR overall. Around 20% of the patients showed a quantifiable benefit from both test mouth-rinses, whereas more than 50% reported a subjective pain reduction. Focusing patients undergoing supportive periodontal therapy, quantifiable effects were found for both test groups. From a patient’s point of view, DHM might be a suitable adjunct to enhance procedural comfort, especially in patients with a history of periodontitis. Clinical relevance The 1-week use of the dentinal-hypersensitivity-reducing mouth-rinses prior to professional-mechanical-plaque-removal showed to be a suitable adjunct to enhance procedural comfort during instrumentation, especially in patients undergoing supportive periodontal therapy. Registration number: DRKS00010811

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