АССОЦИАТИВНЫЕ СВЯЗИ ГЕНА ТИРЕОГЛОБУЛИНА С ПРОДУКТИВНЫМ ДОЛГОЛЕТИЕМ МОЛОЧНОГО СКОТА

Author(s):  
Yu.R. YULMETEVA ◽  
SH.K. SHAKIROV

Изучен полиморфизм гена тиреоглобулина в ассоциации с продолжительностью хозяйственного использования и продуктивностью коров. Генетический профиль популяции по совокупности частот встречаемости аллелей локуса тиреоглобулина выглядит следующим образом выявлено большинство коров-носителей аллеля С в гомозиготном состоянии до 7 лактации, однако увеличение животных с гетерозиготным генотипом наблюдалось в подвыборках 8 и 9 лактаций. В целом по изученной популяции распределение полиморфных генотипов было: TG5CС 63,5, TG5CT 32,1, TG5ТT 4,4 частота встречаемости по аллелям равна: С 0,80, Т 0,20. С увеличением возраста лактации продуктивность различается у коров с разными полиморфными генотипами. У первотелок наибольшая продуктивность установлена у особей с генотипом TG5ТT, от которых надоено на 77 кг больше, чем от гетерозиготных. Однако в дальнейшем высокая продуктивность была характерна для коров с генотипом TG5СС и TG5СT. Средний возраст в лактациях по всему изученному поголовью составляет 2,7. В динамике возраста удой в изученном стаде животных повышался до 7 лактации, максимальные значения которого по ней составили 72947300 кг. Для повышения рентабельности производства молока необходимо принимать меры по повышению продуктивного долголетия.Thyroglobulin gene polymorphism was studied in association with the duration of economic use and productivity of cows. The genetic profile of the population in terms of the frequency of occurrence of alleles of the thyroglobulin locus is as follows - the majority of cows carrying the C allele in a homozygous state up to 7 lactation were detected, however, an increase in animals with a heterozygous genotype was observed in subsamples of 8 and 9 lactations. In general, for the studied population, the distribution of polymorphic genotypes was: TG5CC - 63.5, TG5CT - 32.1, TG5TT - 4.4 the frequency of occurrence in alleles is: C - 0.80, T - 0.20. With increasing age of lactation, productivity differs in cows with different polymorphic genotypes. In heifers, the highest productivity was found in individuals with the TG5TT genotype, from which 77 kg more were fed than from heterozygous ones. However, in the future, high productivity was characteristic for cows with the genotypes TG5CC and TG5CT. The average age in lactations throughout the studied population is 2.7. In the dynamics of age, milk yield in the studied herd of animals increased to 7 lactation, the maximum values of which amounted to 7294-7300 kg. To increase the profitability of milk production, it is necessary to take measures to increase productive longevity.

Author(s):  
S.V. NIKOLAEV ◽  
N.A. SHEMURANOVA

Представлены данные о продуктивности коров холмогорской породы и их помесей с различной степенью голштинизации. В период с 2000 по 2019 год установлено, что в условиях Республики Коми коровы с долей кровности более 75 по голштинской породе превосходят чистопородный холмогорский скот по показателям молочной продуктивности и жира в молоке за 1-ю лактацию на 40,3 и 0,63 абс., а в 3-ю на 52,8 и 0,64 абс., соответственно. Однако повышение молочности за лактацию не приводит к увеличению пожизненного производства молока, что обусловлено снижением продолжительности хозяйственного использования. У коров с долей кровности по голштинам более 75 средний возраст выбытия составляет 2,400,11 отела, что в 1,5 раза меньше по сравнению с чистопородными животными.Наибольший пожизненный удой имеют животные с кровностью 50, полученные при скрещивании чистопородного голштинского и холмогорского скота (19850618 кг), а наименьший с кровностью 2550 при разведении помесей в себе (123281134 кг). Продукция молочного жира за все лактации у коров с кровностью 50 составляет 799,625,3 кг, что в 1,9 раз больше аналогичного показателя животных с долей кровности до 25. При учете молочной продуктивности на 1 день жизни животного установлено, что наибольший показатель наблюдается у коров с кровностью 50 7,4 кг молока натуральной жирности, а при пересчете на базисную жирность (3,4) у высококровных (более 75) по голштинской породе помесей (9,00,3 кг), где также наблюдается наибольший показатель производства молочного жира на 1 день жизни 307,310,1 г.The article presents data on the productivity of cows of the Kholmogorskaya breed and their crosses with varying degrees of holsteinische. In the course of studies conducted in the period from 2000 to 2019, it was found that in the Komi Republic, cows with a blood content of more than 75 of the Holstein breed outperform pure-bred Kholmogorsky cattle in terms of milk productivity and fat in milk for the first lactation by 40.3 and 0.63, and in the third by 52.8 and 0.64 respectively. However, an increase in milk yield per lactation does not lead to an increase in lifetime milk production, which is due to a decrease in the duration of economic use: in cows with a Holstein blood ratio of more than 75, the average leaving age is 2.400.11 calving, which is 1.5 times less than in purebred animals. The highest lifetime yield is given to animals with a blood content of 50 obtained by crossing Chi-pedigree Holstein and Kholmogorsky cattle (19850618 kg), and the lowest with a blood content of 2550 when breeding crossbreeds in themselves (123281134 kg). The production of milk fat during all lactation in cows with a blood content of 50 is 799.625.3 kg, which is 1.9 times more than in animals with a blood content of up to 25. When accounting for milk production on a day in the life of an animal is established that the highest milk yield observed in cows with percentage of the blood 50 and 7.4 kg of milk, natural fat content, and at recalculation on basic fat content (3.4) from 75 of the Holstein breed hybrids (9.00.3 kg), which also has the highest production of milk fat on day 1 of life 307.310.1 grams


2018 ◽  
Vol 51 ◽  
pp. 283-289 ◽  
Author(s):  
N. Mokhnachova ◽  
T. Suprovich ◽  
M. Dobrynska ◽  
N. Fursa

Today conservation of biodiversity is one of the most important priorities in the world. Polymorphism of farm animals is a key to successful breeding; it provides animal adaptation to environmental changes. Impoverishment of genetic resources of farm animals can lead to various negative consequences, such as significant decrease of the effectiveness of selection, existing breeds will not be able to successfully resist to infectious agents evolving constantly; valuable material for the study and analysis of breed origin will be lost. Local breeds created by national selection are valuable genetic resources. Lacking high performance in most cases, they are usually characterized by high resistance to various diseases. Ukrainian Grey cattle, as a representative of the local native breeds, are an interesting object of population research in respect not only of adaptive characteristics, but also genetic mechanisms providing phenotypic expression of certain features of productivity. Recent advances in molecular genetics have made it possible to identify genes associated with qualitative and quantitative characteristics of cattle. The most informative one in this regard is the DNA marker systems based on structural analysis of polymorphisms of genes involved in formation and operation of economically useful traits. The most common potential DNA markers of characteristics of cattle productivity include genes of growth hormone (bGH), beta-lactoglobulin (βLG), thyroglobulin (TG5), calpain (CAPN). BGH gene is an important regulator of somatic growth of animals and has lactating and fat-mobilizing effects. CSN3 gene is linked to milk protein ability and technological properties of milk. Different allelic variants of βLG gene are associated with high content casein and whey proteins in milk, fat percentage and positive impact on milk production. Milk products and falsification detection of milk are controlled by this gene. Its role has been proved in antimicrobial activity to pathogens of mastitis. TG5 gene is a precursor of tyreoid hormones such as triiodothyronine and tetraiodothyronine participating in formation of fat cells and the formation of meat marbling. CAPN gene is involved in proteolysis during ripening of meat and leads to higher tenderness of meat. The aim was to investigate the allelic polymorphism of genes of growth hormone, beta-lactoglobulin, thyroglobulin and calpain in Ukrainian Grey cattle. Blood samples (n = 136) from Ukrainian Grey cattle at "Markeyevo" breeding farm (Kherson region) were studied. Molecular genetic studies were conducted at Laboratory of Genetics of Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of NAAS. DNA isolation from whole blood was performed using standard commercial kit "DNA-Sorb-B" (produced by AmpliSens, Central Research Institute of Epidemiology, Russia). Polymorphisms of genes of GH, βLG5, TG and CAPN1530 was investigated by PCR-RFLP. Electrophoretic separation of restriction fragments of DNA was performed in 1.5% and 2% agarose gels in tris-borate electrophoresis buffer. The results of DNA testing of beta-lactoglobulin locus for A- and B-allele variants in animals of Ukrainian Grey breed found that most of the cows were carriers of homozygous BB genotype of βLG gene. It was determined at every second investigated animals. Homozygous AA genotype was found only at two cows or it is 4%. AB genotype was represented at 45% of animals. Thus, the study found a significant prevalence of frequencies B allele over A allele (0.736 and 0.264, respectively), which leads to overwhelming homozygotisation of one of these alleles. B allele in beta-lactoglobulin gene is associated with high content of casein protein in milk and a high percentage of fat. The values of expected and observed heterozygosity at this gene were not significantly different. The study of gene polymorphism of thyroglobulin found that at the population of the represented breed, the most often seen heterozygous genotype was CT, carriers of which were 57% of the animals. In homozygous state C allele was in 31% of the animals, and T allele – only in 11%. Regarding the level of heterozygosity, we received that actual heterozygosity exceeded theoretically expected one. However, difference is statistically insignificant. Consequently, for thyroglobulin gene Ukrainian Grey cattle belong to breeds that carry the highest frequency of desired C allele for marbling of meat. The analysis of gene polymorphism of bGH indicates the absence of the animals with genotype VV in the investigated sampling, low percentage of heterozygotes (3%) and a significant portion of homozygotes for L allele (98%). Significant differences on levels of actual and expected heterozygosity for somatotropin gene we haven’t found. One of the markers of quality characteristics of meat productivity of cattle is CAPN gene. In general, the animals of Ukrainian Grey breed are characterized by the absence of polymorphism for calpain gene. All the studied animals were carriers of homozygous genotype for preferred G allele (1,0). This feature of the genetic structure of the studied population by calpain gene, animals of which are reproduced in a small array, shows breed-specific high genetic potential by quality characteristic of meat productivity, namely, the tenderness of meat. It was revealed that the population of Ukrainian Grey breed was characterized by low level of polymorphism for genes which cause milk production and meat quality indicators. Thus, homozygous genotype BB dominated for beta-lactoglobulin gene; homozygous LL genotype (98%) was also significantly prevalent and VV genotype was not detected for growth hormone gene; only genotype GG was generally found for calpain gene. And heterozygous CT genotype (57%) dominated only for TG5 locus of thyroglobulin gene.


2021 ◽  
Vol 935 (1) ◽  
pp. 012010
Author(s):  
E D Chirgin ◽  
V G Semenov ◽  
A S Mokretsova ◽  
D S Balitskaya ◽  
E I Ustinova ◽  
...  

Abstract The article presents the results of studies to establish the influence of the record productivity of mothers’ mares on milk yield and the duration of productive life of mares-daughters of the Lithuanian heavy draft breed. It was found that the high level of milk productivity of mothers during the period of highest lactation (up to 4000 kg of milk) enabled their daughters to become leaders already during the first lactation. With an increase in the record milk yields per lactation in mothers, the heritability coefficient in mother-daughter pairs decreased and turned into negative values. The daughters of mares with record milk yields of up to 8000 are the most dairy among their contemporaries. The research results indicate that intensive milk production of mares during the first lactation can cause a reduction in the terms of their economic use due to the large loads on the developing fragile animal organism. The highest rates of productive life, number of lactations and life-long milk yield were obtained from daughters whose mothers had record milk yields per lactation from 7000 kg to 8000 kg of milk.


2017 ◽  
Vol 54 ◽  
pp. 106-112
Author(s):  
Y. I. Sklyarenko ◽  
T. O. Chernyavska ◽  
L. V. Bondarchuk ◽  
I. P. Ivankova

The aim of the research was to study milk production of many factory families of Ukrainian Brown dairy breed and study the influence of origin on the indices of milk yield, duration and effectiveness of lifetime use of cows. Method. For research allocated 9 factory families of Ukrainian Brown dairy breed of State Enterprise «Experimental Farm of Institute of Agriculture of Northern East of NAAS» (Valuty 413, Voli 8369, Laymy 8221, Miry 7209, Ryabuhy 9728, Sujety 6091, Tochky 8169, Chubarochky 7743, Shchepky 4344). Among the indicators of the duration and effectiveness of lifetime use of cows was determined the duration (days) of life, economic use of and of lactation, number of lactations for life, lifetime milk yield and yield of milk fat (kg) average lifetime content (%) fat in milk, milk yield (kg) per day of life, economic use and lactation. The coefficient of economic use was calculated (expression in percent) by the proposed method by Pelehaty M. S. et al. In addition were determined (also in percentage) coefficient of lactation and the coefficient of productive use offered by Polupan Y. P. Milk productivity of the animals was evaluated according to the materials of the primary zoo-technical account. Statistical processing of results of studies was conducted by methods of mathematical statistics using the PC. The results of the research. By analyzing the productivity of cows managed to identify a significant level of specificity and differentiation of families according to the indications. Of the 90 cows accounted for the highest productivity of lactation, 28, or 31%, had hopes of 5.0 thousand kg and above. Most of these cows had family, Miry 7209 (67%), Ryabuhy 9728 (57%), Valuty 413 (55%), Shchepky 4344 (50%), less-Sujety 6091 (36%), Chubarochky 7743 (33%), Tochky 8169 (25%), Laymy 8221 (16%), Voli 8369 (10%). Over a long period of economic use and lactation of animals of Valuty 413 family resulted higher in comparison with other families a lifelong yield. The influence of the origin of cows on the indices of milk productivity and effectiveness for lifelong use was discovered. The origin of the family significantly influenced on milk yield for better lactation and milk yield per 1 day of life. Other indicators were not significant according to the origin, although they had high values (from 5.4 to 26.7%). So we can consider it expedient to work with families to improve the duration and effectiveness of lifetime use of cows. Conclusions. The highest average yield for the first lactation animals is characterized by animals of Ryabuhy 9728 family, the content of fat in milk – by animals of Voli 8369 family. Long average duration and a better efficiency is in the life of the cows of Valuty 413 family. The highest average milk yield in one day life had animals of Valuty 413 family. Animals of Valuty 413 family are allocated at the average value of the coefficient of economic use. Optimal average lactation animals belong to the Voli 8369 and Shchepky 4344 families. In some families identified animals that have a lifetime yield of more than 50.0 thousand kg of milk. The influence of the origin of cows on the indices of milk productivity and effectiveness of lifetime use was installed. Between separate parameters of milk production and indicators of the duration and effectiveness of lifetime use of a set of reliable correlation, the magnitude and direction of which are different in animals of different families.


2021 ◽  
Vol 935 (1) ◽  
pp. 012032
Author(s):  
E D Chirgin ◽  
V G Semenov ◽  
E N Ivanova

Abstract The presented work is the result of studies of the influence of the type of higher nervous activity on the dairy productivity of mares of the Russian heavy draft horse breed on stationary koumiss farms. To determine the types of higher nervous activity, a motor-food technique developed by the All-Russian Research Institute of Horse Breeding was used. The milk yield was counted at animals for the first lactation, for the highest lactation, on average for one lactation and a lifelong milk yield of mares. The milk yield on average for lactation is almost the same in mares with a strong balanced mobile type and with a strong unbalanced type of higher nervous activity. Animals of a weak type and a strong balanced inert type of higher nervous activity lag behind them in this indicator by 11-13%. Horses with a strong balanced mobile type, a strong unbalanced type and a weak type of higher nervous activity are most suitable for the duration of economic use and lifelong milk production. The mares with a strong balanced inert type of higher nervous activity are the least productive in terms of duration of economic use and lifelong milk yield on koumiss farms.


Author(s):  
Рудишина ◽  
Natal'ya Rudishina ◽  
Штырева ◽  
Irina Shtyreva

The productive longevity of dairy cows are affected by many genetic and paratypic factors. An impact of the heifers age at first insemination and the level of milk production in the first lactation on the duration of economic use. The research carried out on the herd of Ob type cows of black-motley breed of Experimental Farm “Prigorodnoe” of Altai Kray. The indicators of 864 cows, which dropped out of the herd from 2010 to 2015, were analyzed. Maximum duration of economic use in lactation was installed in cows, inseminated at the age till 18 months.(6.1 lactation) and from 18.1 to 20 months (5.8 lactations). Cows, inseminated at the age of 26.1 months and more, were used in the herd least - 3.3 lactation. The highest lifetime of milk yield (32 745 kg) was obtained from cows with the age of the first insemination from 18.1 up to 20 months. A correlation analysis revealed a significant effect on the duration of the economic use of the age of the first fruitful insemination of cows, the month and the level of milking cows in the first lactation. The greater the age of the first ful insemination of cows and milk production level of cows during the first lactation, the less the duration of their economic use (respectively r = - 0.28 in the way of p <0.05 and r = - 0,45, p <0.001). The magnitude of life milking yield of cows significantly affects the duration of the economic uses of in lactation (r = 0,89, p <0.001) and less significantly, but significantly, the highest yield of milk per lactation (r = 0,30, p <0.01). It was found that the lower the milk yield of cows during the first lactation, the greater the duration of their economic use in lactation, lifetime milk yield, milk yield per day of life, the yield of milk fat, less than the length of service- and calving periods and the higher the ratio of reproductive ability.


Author(s):  
I. Pishchan

The article set out the materials of researches about the formation of the level of milk production of Austrian cows of Schwyz breed of different generations, depending on the genotype and paratypic factors at their area of ecological origin, considering of which will promote high selection. It was established that in the operation of animals in the area of environmental origin the level of lactic productivity of daughters varies between 5000 – 12000 kg per lactation, whereas their mothers do not exceed maximum index 12,800 kg, at the same time in the mother of father it exceeds 20000 kg. Thus, the qualitative composition of milk from different generations corresponds to the average consolidated of Schwyz breed. The mass part of the fat and protein in the milk of daughters is at the level 4,13 and 3.57%. The highest content of milk fat in mother of father, the mass of fat in the milk is an average of 4.37%. The dynamics of the implementation of milk production of daughters and their mothers on the continuation of the economic use is similar. In the first lactation the total milk yield of cows amounts respectively 6686.9 and 6261.8 kg of milk, in the second lactation, the level rises to 7532.2, and 7748.7 kg. In the future, there is a slight increase in the productivity and after the fourth lactation daughters and their mother’s milk yield is almost identical and is respectively 8138.3 and 81356 kg of milk. The implementation of the genetic potential of the father's mother passes on a higher level, that meet the requirements for this category of parent. In the first lactation from this cows was obtained 9276.3 kg of milk, and the second – 11599.6 kg. The highest level of productivity was observed in the third lactation where milk yield was an average of 11667.5 kg. After the fourth lactation milk yield decreased slightly, but did not fall below the figure 11139.3 kg. It is proved that the realization of the productive potential of the daughters of parents of highly close to that of a mother. If the productivity of the daughters of the average for the entire period of exploitation below the milk yield of their mothers only 87 kg, 4% milk, the index mother of father – to 3762 kg, or 48.5% (P < 0.001).


Author(s):  
С. Л. Войтенко

У статті висвітлені результати досліджень щодо впливу низки чинників на молочну продуктивність корів білоголової української породи, яка відноситься до локальної породи молочного напряму продуктивності й утримується лише в одному племінному господарстві. Формування молочної продуктивності корів даної породи узгоджується з походженням тварин та порядковим номером лактації. Найвищим надоєм за 305 днів першої лактації характеризувалися корови ліній Марта 171 і Озона 417, відповідно 4483,1 та 4254,9 кг молока за варіації ознаки в розрізі ліній у межах 3976,4–4483,1 кг. Встановлено, що надій корів заводського стада підвищується до 5–6 лактацій, даючи змогу збільшити тривалість господарського використання більш високопродуктивних корів за збереження генетичної мінливості ознаки. На можливість підвищення надоїв корів методами селекції вказує коефіцієнт мінливості надоїв корів усіх досліджуваних ліній, окрім Жаргуна 157. Отже, підвищення молочної продуктивності корів української білоголової породи можна здійснювати методами внутрішньопородної селекції, зберігаючи генофонд цієї локальної вітчизняної популяції великої рогатої худоби. The article highlights the results of studies on the impact of factors on milk production of cows of Ukrainian whitehead breed, which belongs to a local dairy breeds productivity and kept only one breeding sector. Formation of milk production of cows of the breed consistent with animal origin and serial number of lactation. Lines Marta 171 and 417 Ozona respectively 4483,1 and 4254,9 kg of milk for signs of variation in terms of lines within 3976,4–4483,1 kg are characterized the highest yields for the first 305 days of lactation cows. It was found that the yield of the plant herd of cows is increased to 5–6 lactation, allowing to increase the duration of the economic use of more productive cows while maintaining the genetic trait variability. On the possibility of increasing the milk yield of cows breeding methods indicates the coefficient of variation of milk yield of cows lines studied, except Zharguna 157. Consequently, the increase of milk productivity of cows of the Ukrainian whitehead breed, you can implement the methods of inbreeding selection, keeping the gene pool of the local national population of cattle.


2021 ◽  
Vol 51 (4) ◽  
pp. 61-66
Author(s):  
S. L. Belozertseva ◽  
L. L. Petrukhina

The results of studies on the effect of the birth season factor on the lifetime productivity and growth rate of cows are presented. The material for the research was the information database of the "Selex" breeding record program for the highly productive herd of black-motley cattle of the "Pre-Baikal type". The research was carried out under the conditions of the Irkutsk region in 20152019. Replacement heifers, which were evaluated for growth and development, and subsequently for milk production during the 1st and 3rd lactations and lifetime production, were chosen as an object of research. Heifers born in the summer-autumn period, according to the 1st lactation, gave the highest productivity, their milk yield was 6151-6179 kg of milk with a fat content of 3.85%. By the 3rd lactation, cows born in the summer months retained high productivity (7001 kg with a milk fat content of 3.83%). From the cows born in winter, a maximum lifetime production of 30,519 kg was obtained. The cows born in spring have 856 kg less lifetime productivity. With the maximum lifelong productivity, the age in lactations was 4.5, with the minimum - 4.0. This indicates that the shorter the period of productive use, the lower the lifetime milk yield of cows. The cows that gave the highest milk production in the first lactations dropped out of the herd sooner for various reasons, thereby not having reached their full genetic potential and not having shown their possible maximum milk production.


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