scholarly journals Detection of Cortisol Level via Enzyme Immunoassay Influenced by Exercise

2019 ◽  
pp. 23-27
Keyword(s):  
Enzyme Immunoassay ◽  
Cortisol Level ◽  
Primary Function ◽  
Significant Difference ◽  
Before And After ◽  
Cortisol Levels ◽  
Female Subjects

Cortisol is among the human body’s most necessary hormones. On any given day, cortisol’s primary function is to control how our body creates and utilizes energy. This study examined the influence of exercise on the cortisol levels.Twenty female subjects had their cortisol levels determined before their morning exercise (Pre) and after their morning exercise (Post) using microplate enzyme immunoassay method. The result of study showed that there was significant difference (P<0.05) in the cortisol levels before exercise 13.49 ± 0.90µg/dl Compared with after exercise 15.10±1.08µg/dl. It was also observed that there was no significant difference in the cortisol level (P>0.05) obtained from the female subjects before and after exercise between the ages of 21-23, 24- 26 and 27-29 years. Collectively, these results showed that exercise provoked increased in cortisol level. Keyword: Cortisol, Immunoassay, Exercise, Hormone

scholarly journals Stress and Anxiety in Children After the Use of Computerized Dental Anesthesia

2015 ◽  
Vol 26 (3) ◽  
pp. 303-307 ◽  
Author(s):  
Alexandra M. Queiroz ◽  
Ariany B. Carvalho ◽  
Laís L. Censi ◽  
Carmen L. Cardoso ◽  
Christie R. Leite-Panissi ◽  
...  
Keyword(s):  
Pediatric Patients ◽  
Numerical Data ◽  
Anxiety State ◽  
Significance Level ◽  
Dental Anesthesia ◽  
Psychological Analysis ◽  
Significant Difference ◽  
Before And After ◽  
Cortisol Levels ◽  
To Receive

The aim of this study was to evaluate the transitory stress levels and the anxiety state in children submitted to conventional and computerized dental anesthesia. Twenty children (7 to 12 years) were randomly assigned to receive conventional and computerized dental anesthesia. To investigate the hypothesis that transitory stress could be lower after using computerized anesthesia compared to conventional anesthesia, cortisol levels in saliva were measured before and after each technique. Anxiety was also evaluated individually by answering the State-Trait Anxiety Inventory for Children (STAIC). Numerical data were analyzed statistically by the Mann-Whitney non-parametric test (5% significance level). Salivary cortisol levels increased in 8 (40%) patients after conventional anesthesia and in 9 (45%) patients after computerized anesthesia, with no statistically significant difference between the two types (p=0.34). In the same way, no statistically significant difference was found between the techniques (p=0.39) related to the psychological analysis based on the STAIC scores. Local anesthesia using either conventional anesthesia or a computerized delivery system produced similar level of stress/anxiety in pediatric patients, using both quantitative and qualitative analyses.

scholarly journals Salivary Cortisol as a Biomarker of Stress in Mothers and their Low Birth Weight Infants and Sample Collecting Challenges

2016 ◽  
Vol 35 (2) ◽  
pp. 118-122 ◽  
Author(s):  
Milica Ranković Janevski ◽  
Ana Đorđević Vujičić ◽  
Svjetlana Maglajić Đukić
Keyword(s):  
Cortisol Level ◽  
Stress Reduction ◽  
Salivary Cortisol ◽  
High Sensitivity ◽  
Sample Volume ◽  
Low Birth Weight Infants ◽  
Skin Contact ◽  
Before And After ◽  
Cortisol Levels ◽  
The Difference

Summary Background: Salivary cortisol measurement is a non-invasive method suitable for use in neonatal research. Mother-infant separation after birth represents stress and skin-to-skin contact (SSC) has numerous benefits. The aim of the study was to measure salivary cortisol in mothers and newborns before and after SSC in order to assess the effect of SSC on mothers’ and infants’ stress and to estimate the efficacy of collecting small saliva samples in newborns. Methods: Salivary cortisol was measured in 35 mother-infant pairs before and after the first and the fifth SSC in small saliva samples (50 μL) using the high sensitivity Quantitative ELISA-Kit (0.0828 nmol/L) for low cortisol levels detection. Samples were collected with eye sponge during 3 to 5 minutes. Results: Cortisol level in mothers decreased after SSC: the highest levels were measured before and the lowest after SSC and the differences in values were significant during both the first (p<0.001) and the fifth SSC (p<0.001). During the first SSC the cortisol level decrease was detected in 14 (40%) and an increase in 21 (60%) newborns, and during the fifth SSC a decrease was detected in 16 (45.7%) and an increase in 19 (54.3%) newborns, without confirmed significance of the difference. Saliva sampling efficacy using eye sponge was 75%. Conclusions: Cortisol level decrease in mothers proves the stress reduction during SSC, while variable cortisol levels in infants do not indicate stress reduction and imply the need for further research. The used sampling method appeared to be one of the most optimal considering the sample volume, sampling time and efficacy.

scholarly journals Changes in Cortisol Levels before and after Supportive Psychotherapy in Patients with Comorbid Cervical Cancer Distress with Depression Type

2018 ◽  
pp. 179
Author(s):  
Laila Nuranna ◽  
Kartiwa H Nuryanto ◽  
Andriansyah Andriansyah ◽  
Sylvia D Elvira ◽  
Bambang Sutrisna
Keyword(s):  
Cervical Cancer ◽  
Treatment Group ◽  
Cortisol Level ◽  
Distress Thermometer ◽  
Control Group ◽  
P Value ◽  
Supportive Psychotherapy ◽  
Average Value ◽  
Significant Difference ◽  
Before And After

Objective: To prove the success of supportive psychotherapy thatwas provided as a distress therapy on advanced cervical cancer.Knowing the prevalence of distress type of depression in patientwith epithelial cervical cancer, proving the benefits of psychotherapysupportive for distress can decreasing cortisol level in cervicalcancer patient, can assess distress thermometer score, HAM-D17score and scoring incident predictors of distress with depressiontype.Methods: There were 32 subjects from 71 advanced cervical cancerpatients had mild-moderate depression. Then randomizationblocking was performed to determine a subject who entered thetreatment group (n = 16) who got supportive psychotherapy orcontrol group who got common psychotherapy (n = 16). All ofparticipants assessed the distress level with cortisol value, distressthermometer score, and HAM-D17 score before and after they gotsupportive psychotherapy.Results: After the intervention of psychotherapy in the treatmentgroup decreased HAM-D17 score, the average decline 7.53 (SB 3.34).The mean decreasing in the control group was 3.98 (SB 2.85). Thereis a significant difference in mean reduction in HAM-D17 scores ontreatment and control groups with p = 0.003 (p <0.005). There wasdecreasing blood cortisol level in the treatment group amounted to39.43, while the control group there was a drop of 1.59. The reductionof cortisol level in the treatment group and the control has a pvalue0.302. After got supportive psychotherapy, found a decreasingthe average value of the thermometer distress in the treatmentgroup 3.02 and the control group 2.51, with a p value more than0.492.Conclusion: There were 45% of cervical cancer patients in the clinicexperiencing distress disorder with depressive type. The bloodcortisol level could be decreased by giving supportive psychotherapywith a mean decrease of 39.43 nmol/l. There was a significantreduction in the level of depression (HAM-D17 score) of 7.53 pointsand distress thermometer impairment by 3 points after givensupportive psychotherapy. Obtained scoring predictors for theoccurrence of distress type of depression in patients with advancedcervical cancer with a sensitivity of 46.15% and a specificity of89.47%.[Indones J Obstet Gynecol 2018; 6-3: 179-187]Keywords: cervical cancer, cortisol, distress, distress thermometer,HAM-D17 score

scholarly journals Effect of salat (prayer) activity on salivary status and cortisol level

2019 ◽  
Vol 5 (2) ◽  
pp. 51
Author(s):  
Nadia Rully Auliawati ◽  
Layung Sekar Prabarayi ◽  
Rais Aliffandy Damroni ◽  
Juni Handajani
Keyword(s):  
Oral Health ◽  
Cortisol Level ◽  
Flow Rate ◽  
Salivary Flow ◽  
The Body ◽  
Salivary Flow Rate ◽  
Significant Difference ◽  
Salivary Ph ◽  
Cortisol Levels ◽  
Normal Acidity

The majority of Indonesian people are Muslim who perform the obligatory prayers five times each day known as salat. The series of activities in salat begins with wudu with a gargle movement that increases salivary secretion through mechanical stimulation. The movement of ruku and sujood when salat causes oxygen-rich blood to flow to the brain is well suited. When a person feels calmer and more psychically relaxed after performing salat and wudu, the parasympathetic nerves increase so normal cortisol levels can secrete more saliva which is aqueous with normal acidity (pH). Oral health will affect the overall health of the body. This study aimed to determine the effect of salat and wudu on the salivary flow rate, pH, and cortisol level as an indicator of oral health. The design of this study was the static group pretest and posttest design. The study was conducted by comparing the value of salivary flow rate, pH, and cortisol levels in 20 subjects consisting of 10 subjects as the treatment group and 10 subjects as the control. Saliva was collected without stimulation before and after salat activity for five minutes, followed by measurement of salivary flow rate and pH level. Salivary cortisol level was measured using Cortisol Assay Elisa kit with λ 540 nm. The results showed an increase in salivary pH, flow rate and cortisol level after the salat activity but there was no significant difference by Kruskal-Wallis test but there was a strong correlation using Kendall’s tau_b test. In conclusion, salat activity may have correlation with an increase in salivary pH, flow rate and cortisol level.

scholarly journals The Comparison of Plasma Cortisol Levels between 0.125% Bupivacaine and 5 mg Continuous Oxycodone in Lower Extremity Orthopedic Surgery

2022 ◽  
Vol 2 (2) ◽  
pp. 222-242
Author(s):  
Zulkifli ◽  
Agustina Br Haloho ◽  
Legiran ◽  
Pirma I.R.M
Keyword(s):  
Cortisol Level ◽  
Orthopedic Surgery ◽  
Lower Limb ◽  
Pain Perception ◽  
System Response ◽  
Double Blind Study ◽  
Double Blind ◽  
Significant Difference ◽  
Duration Of Surgery ◽  
Cortisol Levels

Introduction: Pain is a problem often encountered in postoperative patients. Study has shown after a procedure, 80% patients experience acute pain. This postoperative pain will affect patient’s quality of life therefore necessitating quick and proper treatment. Tissue trauma during surgery will have influence on body system, including endocrine. One of endocrine system response is cortisol secretion. Cortisol levels may be attenuated by bupivacaine and oxycodone. The aim of this study was to determine the efficacy of bupivacaine0,125% and oxycodone 5 mg on pain perception measured by cortisol in patients undergoing orthopedic surgery of the lower limb. Methods: A randomized clinical trial, double-blind study was performed at Mohammad Hoesin General Hospital in Palembang, South Sumatra, from November to May 2021. There were forty samples and divided into two groups (bupivacaine 0,125% and oxycodone 5 mg). Groups were divided by block randomization by computerized random number generator. Blinding were done by making analgesic has the same packages to prevent knowledge of which intervention is being done. Data were analyzed using independent t-test, ANOVA, Mann-Whitney and Chi Square with SPSS version 22.0. Results: There were no statistically significant differences between the two groups on age, gender, body mass index and duration of surgery. In bupivacaine group, cortisol level decreased from 12.94±6.99 µg/dl to 11.32±5.42 µg/dl meanwhile oxycodone group cortisol levels increased from 11.81±8.47 µg/dl to 11.82±7.56 µg/dl. There were no significant difference between two groups relating to cortisol levels. Conclusions: No significant difference was found on administration of bupivacaine 0,125% and oxycodone 5 mg as epidural analgesia on cortisol level in postoperative orthopedic surgery of the lower limb patient.

scholarly journals Association of Psoriasis Severity with Serum Prolactin, Thyroid Hormones, and Cortisol before and after Treatment

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Reza M. Robati ◽  
Parviz Toossi ◽  
Mohammad Rahmati-Roodsari ◽  
Sara Khalilazar ◽  
Ehsan Abolhasani ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Serum Cortisol ◽  
Severity Index ◽  
Thyroid Stimulating Hormone ◽  
Serum Prolactin ◽  
Significant Difference ◽  
Before And After ◽  
Pasi Score ◽  
Cortisol Levels ◽  
Normal Controls

Background. Prolactin (PRL) level is proposed to be associated with the severity of psoriasis although the previous studies reported different results.Objective. To find the association between PRL levels and severity of psoriasis before and after treatment. In addition, we aimed to find a difference in prolactin, thyroid stimulating hormone (TSH), thyroid hormones (T3and T4), and cortisol levels between patients with psoriasis and normal controls.Methods. First, the levels of hormones were measured in 30 patients with psoriasis and 30 matched controls. The severity was assessed by psoriasis area and severity index (PASI). Then, patients were treated, and PASI was assessed every week until achieving PASI-75 response. At this time, the hormones were measured again and compared to the baseline.Results. No statistical significant difference was observed in the mean PRL, T3, T4, TSH, and cortisol levels between cases and controls. Comparing to the baseline, a significant decrease in PRL levels and a significant increase in T3and serum cortisol levels were observed after treatment (P<0.05), while the changes in other hormones were not significant.Conclusion. After treatment, PRL significantly decreased, and T3and cortisol levels significantly increased. No correlation between hormone levels and improvement of PASI score existed.

scholarly journals Impact of Fluconazole Prophylaxis on Cortisol Levels in Critically Ill Surgical Patients

2004 ◽  
Vol 48 (7) ◽  
pp. 2471-2476 ◽  
Author(s):  
Shelley S. Magill ◽  
Thanyawee Puthanakit ◽  
Sandra M. Swoboda ◽  
Kathryn A. Carson ◽  
Roberto Salvatori ◽  
...  
Keyword(s):  
Critically Ill ◽  
Cortisol Level ◽  
Fungal Infections ◽  
Case Reports ◽  
Surgical Patients ◽  
Significant Difference ◽  
Cortisol Levels ◽  
Fluconazole Prophylaxis ◽  
Critically Ill Surgical Patients ◽  
Over Time

ABSTRACT Fluconazole is widely used in the intensive care unit for prevention and treatment of fungal infections. Case reports have described an association between fluconazole and adrenal dysfunction, an important cause of morbidity and mortality in critically ill patients. We sought to determine whether 400 mg of fluconazole per day administered to critically ill surgical patients was associated with a reduction in cortisol levels. Cortisol levels were measured in stored plasma specimens drawn from 154 critically ill surgical patients randomized in 1998-1999 to receive fluconazole or placebo for the prevention of candidiasis. The primary outcome measure was the median plasma cortisol level ≥1 day after study drug initiation (MPCL). Secondary outcomes were adrenal dysfunction, defined as an MPCL of <15 μg/dl, changes in cortisol levels over time, and mortality. The median MPCL was 15.75 μg/dl (interquartile range [IQR], 11.65 to 21.33 μg/dl) in 79 patients randomized to fluconazole and 16.71 μg/dl (IQR, 11.67 to 23.00 μg/dl) in 75 patients randomized to placebo (P = 0.52). Patients randomized to fluconazole did not have significantly increased odds of adrenal dysfunction compared to patients randomized to placebo (odds ratio, 0.98; 95% confidence interval, 0.48 to 2.01). Randomization to fluconazole was not associated with a significant difference in cortisol level changes over time. Mortality was not different between patients with and without adrenal dysfunction, nor was it different between patients with adrenal dysfunction who were randomized to fluconazole and those randomized to placebo. Fluconazole prophylaxis in this population of critically ill surgical patients did not result in significant adrenal dysfunction.

scholarly journals The effect of post-abortion care (PAC) on anxiety in women with spontaneous abortion based on MicroRNA-21 expression, cortisol level, and Fordyce happiness pattern

2022 ◽  
Vol 67 (4) ◽  
pp. 181-188
Author(s):  
Leilei Gong ◽  
Jun Han ◽  
Wenwen Yan ◽  
Yichen Qin
Keyword(s):  
Spontaneous Abortion ◽  
Cortisol Level ◽  
Intervention Group ◽  
Biological Factors ◽  
Abortion Care ◽  
Post Abortion Care ◽  
Significant Difference ◽  
The Mean ◽  
Cortisol Levels ◽  
Post Abortion

Abortion is one of the most common complications in pregnancy, and the cause of its occurrence in many cases remains unknown. The high prevalence and consequences of anxiety in women with spontaneous abortion could highlight the importance and role of post-abortion care (PAC). Detection and identification of biomarkers related to abortion and anxiety can effectively diagnose and prevent complications. Among the known biomarkers, microRNAs and the cortisol level have high potential. Therefore, the present study evaluated the effect of post-abortion care (PAC) on anxiety in women with spontaneous abortion based on MicroRNA-21 expression, cortisol level, and Fordyce happiness pattern. In this randomized clinical trial, 72 women with spontaneous abortion were studied and randomly divided into two groups of intervention (n = 36) and control (n = 36). Data were collected through a demographic questionnaire and HADS. To assess PAC, the intervention group was consulted in 8 sessions of 60 minutes in the first 72 hours after abortion. Meetings were held twice a week for four weeks. Both groups were followed up immediately after and one month after the intervention. To evaluate biological factors, 4ml of blood sample was obtained from the subjects. Blood cortisol levels were measured by the Cortisol Competitive Human ELISA Kit (Thermo-Fisher, USA), and microRNA-21 evaluation was performed by Real-time PCR technique. Data were analyzed using SPSS16 software. Results showed that before the intervention, there was no significant difference in the mean score of anxiety between the control and intervention groups (P> 0.05); But at the time immediately and one month after the intervention, there was a significant difference in the mean score of anxiety (p <0.001). The results of biological factors evaluation showed that in the intervention group, serum cortisol levels and microRNA-21 expression decreased significantly (p <0.05). In general, PAC based on the happiness pattern can control the anxiety of women with spontaneous abortion. Therefore, it is recommended as an effective and non-invasive intervention in preventing women's psychological problems after spontaneous abortion.

scholarly journals The Acute Effect of Aerobic Exercise on Serum Cortisol Levels of Athletes and Sedentary Individuals

2018 ◽  
Vol 6 (12a) ◽  
pp. 29
Author(s):  
Selahattin Koc
Keyword(s):  
Aerobic Exercise ◽  
Anaerobic Respiration ◽  
Serum Cortisol ◽  
Acute Effect ◽  
Blood Parameters ◽  
Post Exercise ◽  
Blood Samples ◽  
Significant Difference ◽  
Before And After ◽  
Cortisol Levels

The purpose of this study was to investigate the effect of post-challenge acute values of serum cortisol levels in elite and sedentary athletes before aerobic exercise. This study included 22 elite middle-distance runners from various clubs around Turkey and 16 voluntary non-athlete men. The Cooper test was applied to study subjects. Heart rate values and blood samples of the subjects were taken before and after the Cooper test. The blood samples were analyzed at Central Biochemistry Laboratory of Şahinbey Research and Application Hospital of Gaziantep University. The results showed statistically significant difference between sedentary and athlete groups in comparison of BMI (P<0.05). A statistically significant difference was also found in pre-exercise cortisol and potassium levels and post-exercise lactate and potassium levels between both groups (P<0.05). No statistically significant difference was detected between groups in comparison of other blood parameters (P>0.05). Again, no statistically significant difference was found between pre-test and post-test values of pre and post-exercise Cortisol and Potassium levels of both groups (P>0.05).Consequently, cortisol levels were same in both groups and were not affected by exercise. According to these results, it can be said that athletes’ volume of air entering the lungs is higher than those who do not do sports, and the level of their anaerobic respiration is lower.

scholarly journals Study of serum cortisol levels in complicated and uncomplicated Plasmodium vivax malaria patients

2020 ◽  
Vol 9 (1) ◽  
pp. 254
Author(s):  
Sandeep B. R. ◽  
M. G. Bhutto ◽  
Suresh Babu K. P.
Keyword(s):  
Plasmodium Vivax ◽  
Cortisol Level ◽  
Vivax Malaria ◽  
Serum Cortisol ◽  
Treatment Modalities ◽  
Serum Cortisol Level ◽  
Control Group ◽  
Significant Difference ◽  
Plasmodium Vivax Malaria ◽  
Cortisol Levels

Background: Malaria results in pathological changes in various body organs, as the parasite invade and multiply in circulating red blood cells. Despite of advances in diagnostic and treatment modalities, worldwide incidences of malaria are significant. Current study was conducted to investigate serum cortisol level changes as a promising biomarker for risk prediction in malaria and to study adrenal insufficiency in malaria patients.Methods: Current investigation was a prospective observational study, conducted on complicated and uncomplicated Plasmodium vivax malaria patients. Serum cortisol levels in patients were investigated through immunoassay using direct chemiluminescent technology and were statistically correlated with Plasmodium vivax malaria infection.Results: Results of present investigation revealed that on day 1 there was significant difference in mean serum cortisol levels between the Plasmodium vivax malaria patients and control group and cortisol levels were significantly higher in complicated Plasmodium vivax malaria patients compared to uncomplicated cases on day 1 and 7. Cortisol levels were observed to be normal on day 1 and 7 in uncomplicated malaria cases and in patients with bleeding manifestations, renal failure and jaundice. In 10 out of 15 cases of cerebral malaria, significant increase in serum cortisol levels were observed on day 1, while on day 7 levels were normal in all 15 cases.Conclusions: Rise in serum cortisol level had a positive correlation with temperature and thus can be useful to predict the severity of disease in Plasmodium vivax malaria patients. No cortisol insufficiency was observed in during active and convalescent stages of illness.

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