scholarly journals Salivary Cortisol as a Biomarker of Stress in Mothers and their Low Birth Weight Infants and Sample Collecting Challenges

2016 ◽  
Vol 35 (2) ◽  
pp. 118-122 ◽  
Author(s):  
Milica Ranković Janevski ◽  
Ana Đorđević Vujičić ◽  
Svjetlana Maglajić Đukić
Keyword(s):  
Cortisol Level ◽  
Stress Reduction ◽  
Salivary Cortisol ◽  
High Sensitivity ◽  
Sample Volume ◽  
Low Birth Weight Infants ◽  
Skin Contact ◽  
Before And After ◽  
Cortisol Levels ◽  
The Difference

Summary Background: Salivary cortisol measurement is a non-invasive method suitable for use in neonatal research. Mother-infant separation after birth represents stress and skin-to-skin contact (SSC) has numerous benefits. The aim of the study was to measure salivary cortisol in mothers and newborns before and after SSC in order to assess the effect of SSC on mothers’ and infants’ stress and to estimate the efficacy of collecting small saliva samples in newborns. Methods: Salivary cortisol was measured in 35 mother-infant pairs before and after the first and the fifth SSC in small saliva samples (50 μL) using the high sensitivity Quantitative ELISA-Kit (0.0828 nmol/L) for low cortisol levels detection. Samples were collected with eye sponge during 3 to 5 minutes. Results: Cortisol level in mothers decreased after SSC: the highest levels were measured before and the lowest after SSC and the differences in values were significant during both the first (p<0.001) and the fifth SSC (p<0.001). During the first SSC the cortisol level decrease was detected in 14 (40%) and an increase in 21 (60%) newborns, and during the fifth SSC a decrease was detected in 16 (45.7%) and an increase in 19 (54.3%) newborns, without confirmed significance of the difference. Saliva sampling efficacy using eye sponge was 75%. Conclusions: Cortisol level decrease in mothers proves the stress reduction during SSC, while variable cortisol levels in infants do not indicate stress reduction and imply the need for further research. The used sampling method appeared to be one of the most optimal considering the sample volume, sampling time and efficacy.

scholarly journals Assessment and Correction of Stress in Preterm Infants and Their Mothers

2021 ◽  
Author(s):  
Iryna Sarapuk ◽  
Halyna Pavlyshyn
Keyword(s):  
Preterm Infants ◽  
Cortisol Level ◽  
Salivary Cortisol ◽  
Kangaroo Mother Care ◽  
Overall Design ◽  
Skin Contact ◽  
Salivary Cortisol Level ◽  
Baseline Response ◽  
Cortisol Levels ◽  
Skin To Skin

Abstract The objective was to study the peculiarities of hormonal stress in preterm infants in the NICU and their mothers, and to investigate how skin-to-skin contact influences it. The study included 60 preterm infants with GA˂32 weeks who were treated in NICU and their mothers. The overall design was a baseline-response design. Saliva was collected before (baseline) and after skin-to-skin contact to measure cortisol by enzyme immunoassay method. Infant baseline salivary cortisol level was 0.587 [0.239; 1,714] µg/dL. It was significantly higher in neonates who had invasive ventilation (1.00 [0.38; 2.44]) µg/dL compared to non-ventilated infants (0.335 [0.156; 1.236]) µg/dL, p = 0.022; and positively correlated with the Apgar score at 1st and 5th minutes (r=-0.36; p = 0.011 and r=-0.34; p = 0.017) and duration of ventilation (r = 0.70; p = 0.0000). Maternal baseline salivary cortisol level was 0.212 [0.123; 0.378]µg/dL. There was significant decreasing of salivary cortisol after Kangaroo mother care with skin-to-skin contact in infants (0.162 [0.111; 0.231]) µg/dL, p˂0.001 and mothers (0.096 [0.077; 0.156]) µg/dL, p˂0.001. Conclusion: Preterm infants in the NICU and their mothers experience stress confirmed by the increased baseline cortisol levels. Kangaroo mother care with skin-to-skin contact reduces the stress and normalizes salivary cortisol levels in both infant and mother in the NICU.

Effects of choral singing on salivary cortisol levels and self-reported stress in university students

2021 ◽  
Vol 13 (4) ◽  
Author(s):  
Gunnar Glauco De Cunto Carelli Taets ◽  
Ramon Werner Heringer Gutierrez ◽  
Leila Brito Bergold ◽  
Luana Silva Monteiro
Keyword(s):  
University Students ◽  
Salivary Cortisol ◽  
Health Issues ◽  
Group Approach ◽  
Choral Singing ◽  
Before And After ◽  
Quasi Experimental ◽  
Potential Impact ◽  
Cortisol Levels

Although mental disorders are common among university students, the majority of students with mental health issues do not seek out treatment during their academic life. The aim of this study was to evaluate the effects of a novel group approach- choir singing, and its potential impact on stress, via salivary cortisol levels and self-reported stress levels in university students during 4 sessions of choir singing. It was based on a quasi-experimental research conducted with 25 Brazilian university students. Each choir singing session lasted 60 minutes. A question was asked before and after the intervention: what is the level of your stress? Salivary cortisol was collected before and after the intervention. After the choir singing intervention, there was  a statistically significant reduction in self-reported stress (p<0.0001) and salivary cortisol levels (p<0.0001). This research suggests that choir singing can reduce the self-reported stress of university students after 4 weeks of follow-up with ∆= 45.83% and salivary cortisol levels in the first week with ∆= 3.57%.

Regularities of changes in microhardness in the volume of EDT-69N binder cured at different temperatures

2020 ◽  
Vol 4 ◽  
pp. 65-71
Author(s):  
E.A. Veshkin ◽  
◽  
V.I. Postnov ◽  
V.V. Semenychev ◽  
E.V. Krasheninnikova ◽  
...  
Keyword(s):  
Cross Section ◽  
High Sensitivity ◽  
Sample Volume ◽  
Curing Temperature ◽  
Molding Temperature ◽  
Parabolic Law ◽  
Dimensionless Coefficient ◽  
Straight Line ◽  
Different Temperatures ◽  
The Difference

The change in the microhardness over the thickness of samples made of EDT-69N binder cured in vacuum and at atmospheric pressure at temperatures from 130 to 170°C was investigated. It was found that the change in microhardness along the thickness of the samples occurs according to the parabolic law, with the maximum values being achieved in the middle of the sample cross-section along the thickness. With an increase in the molding temperature, the microhardness in the middle section of the sample increases from 222 MPa at a molding temperature of 130°C to 410 MPa during molding at 170°C. At the critical molding temperature (170°C), the microhardness in all zones of the specimen cross section (subsurface, semi-average, and core) levels off, while the parabolic dependence degenerates into a straight line. It is shown that the method of scratching (sclerometry) demonstrated a sufficiently high sensitivity to the state of samples cured at different temperatures. With an increase in the molding temperature, the width of the sclerometric grooves decreases. At a critical molding temperature of 170°C, the groove width is stabilized and becomes constant throughout the sample thickness. To characterize the difference in the values of the microhardness of the cured binder in the sample volume, it is proposed to use a dimensionless “coefficient of volume anisotropy,” which can take a positive, negative or zero value. With an increase in the curing temperature of the binder and, accordingly, with an increase in the microhardness of the sample, the coefficient of volume anisotropy decreases, and when the samples are molded at the critical temperature, it turns to zero, which indicates the absence of anisotropy.

scholarly journals Correlation between salivary cortisol levels and dental anxiety in children of smokers and nonsmokers

2017 ◽  
Vol 11 (02) ◽  
pp. 192-195
Author(s):  
S. Pavani Reddy ◽  
M. Ghanashyam Prasad ◽  
A. Naga RadhaKrishna ◽  
Kaniti Saujanya ◽  
N. V. K. Raviteja ◽  
...  
Keyword(s):  
Salivary Cortisol ◽  
Dental Anxiety ◽  
Smoking Habit ◽  
Negative Influence ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Population ◽  
Cortisol Levels ◽  
The Difference ◽  
Group 2

ABSTRACT Objectives: The present study was undertaken to evaluate salivary cortisol levels in children of smokers and nonsmokers and thereby establishing the relationship between cortisol levels in response to anxiety in children based on their father's habit of smoking. Materials and Methods: The study population aged between 8 and 10 years includes two groups. Group 1 is comprised 20 children of cigarette smokers and Group 2 is comprised 20 children of nonsmokers. The passive drooling technique was used to collect unstimulated saliva from the children using a sterile container. Salivary cortisol levels were evaluated using Enzyme-linked Immunosorbent Assay method. The obtained data were subjected to statistical analysis using SPSS software and paired t-test. Results: Higher mean salivary cortisol levels were found in children of smokers compared to children of nonsmokers and the difference between them was significant statistically (P < 0.05). Higher salivary cortisol levels were found in females compared to males and the result was significant statistically (P < 0.05). Conclusion: This study has proved that the smoking habit of the father has a negative influence on the anxiety levels of their children.

Examination Stress, Salivary Cortisol, and Academic Performance

2003 ◽  
Vol 93 (3_suppl) ◽  
pp. 1133-1134 ◽  
Author(s):  
Vivian Ng ◽  
David Koh ◽  
Sin-Eng Chia
Keyword(s):  
Academic Performance ◽  
Graduate Students ◽  
Perceived Stress ◽  
Salivary Cortisol ◽  
Final Examination ◽  
Before And After ◽  
Examination Stress ◽  
Cortisol Levels

Immediately before and after participation in a written final examination, 11 graduate students rated their self-perceived stress and provided saliva samples for cortisol assay. Students rated stress higher before the examination, and these ratings were associated with increased salivary cortisol. Students who reported higher stress and had higher cortisol levels before the examination tended to have significantly lower examination scores.

Comparison of Behavior and Dental Anxiety During Intranasal and Sublingual Midazolam Sedation – A Randomized Controlled Trial

2016 ◽  
Vol 40 (1) ◽  
pp. 81-87 ◽  
Author(s):  
AK Shanmugaavel ◽  
Sharath Asokan ◽  
John J Baby ◽  
Geetha Priya ◽  
J Gnana Devi
Keyword(s):  
Drug Administration ◽  
Salivary Cortisol ◽  
Anxiety Level ◽  
Anxiety Scale ◽  
Sublingual Administration ◽  
Before And After ◽  
Group A ◽  
Anxiety Levels ◽  
Cortisol Levels ◽  
Group B

Objectives: The objective of the study was to assess the behavioral effects and the changes in the anxiety level of children after intranasal and sublingual midazolam sedation using Venham's clinical anxiety scale and salivary cortisol level. Study Design: Twenty children aged 3 to 7 years were randomly assigned to Group A (n=10) intranasal or Group B (n=10) sublingual midazolam (0.2mg/kg) sedation. The anxiety levels at various time periods were assessed using Venham clinical anxiety scale and corresponding changes in salivary cortisol levels were assessed before and after the drug administration. The anxiety levels were assessed independently by two pediatric dentists from recorded videos. Wilcoxon signed rank test and Mann Whitney U test were used for statistical analysis using SPSS version 19.0.Results: There was a significant decrease in anxiety level from baseline to 20 minutes after drug administration in group A (p=0.004) and group B (p=0.003). There was no significant change in salivary cortisol levels before and after the drug administration in group A (p=0.07) and group B (p=0.38). Conclusions: Both intranasal and sublingual administration of midazolam was equally effective in reducing the child's anxiety. However, there was no significant difference in the salivary cortisol levels in both groups.

scholarly journals Detection of Cortisol Level via Enzyme Immunoassay Influenced by Exercise

2019 ◽  
pp. 23-27
Keyword(s):  
Enzyme Immunoassay ◽  
Cortisol Level ◽  
Primary Function ◽  
Significant Difference ◽  
Before And After ◽  
Cortisol Levels ◽  
Female Subjects

Cortisol is among the human body’s most necessary hormones. On any given day, cortisol’s primary function is to control how our body creates and utilizes energy. This study examined the influence of exercise on the cortisol levels.Twenty female subjects had their cortisol levels determined before their morning exercise (Pre) and after their morning exercise (Post) using microplate enzyme immunoassay method. The result of study showed that there was significant difference (P<0.05) in the cortisol levels before exercise 13.49 ± 0.90µg/dl Compared with after exercise 15.10±1.08µg/dl. It was also observed that there was no significant difference in the cortisol level (P>0.05) obtained from the female subjects before and after exercise between the ages of 21-23, 24- 26 and 27-29 years. Collectively, these results showed that exercise provoked increased in cortisol level. Keyword: Cortisol, Immunoassay, Exercise, Hormone

scholarly journals Effect of Aquarobic and Weight Training on Cortisol Levels in Obese Women

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Siti Baitul Mukarromah ◽  
Hardhono Susanto ◽  
Tandiyo Rahayu
Keyword(s):  
Weight Training ◽  
Physical Stress ◽  
Control Group ◽  
Obese Women ◽  
Control Group Design ◽  
Handling Stress ◽  
Before And After ◽  
Cortisol Levels ◽  
The Difference ◽  
And Control

Exercise is physical stress which potentially causes disruption of homeostasis, especially in sports that is excessively done. Weight Training (LB) and Aquarobic Exercise (LA) can be modulators of handling stress. This research aims at investigating the effect of the difference between LB and LA to physical stress in obese women. The study was conducted in 2014. The method used in this study was randomized experimental pretest-posttest control group design in 36 obese women, aged 45-50 years who were divided into 3 groups, group LB 50% RM, 3 sets, 12 repetition, treatment two times a day for 8 weeks (n = 12), LA 75% HRmax, treatment 2 days for 8 weeks (n = 12) and control group (n = 12). Body Mass Index (BMI) and cortisol levels were measured before and after the treatment. Hypothesis testing was conducted using test (One-Way ANOVA and Kruskal-Wallis) and the mean difference test (Tukey HSD and Mann Whitneys). The results of BMI is increased in the WT group and is decreased in LA group as compared to control group (p <0.05). The decrease of cortisol level is higher than in LA and LB group and controls (p <0.05). LB and LA affect the physical stress that is characterized by the increase in cortisol levels in obese women. Conclusion: LB is more dominant than LA in increasing physical stress.

scholarly journals Cortisol, Temperament and Serotonin in Karate Combats: An Evolutionary Psychobiological Perspective

2021 ◽  
Author(s):  
Davide Ponzi ◽  
Harold Dadomo ◽  
Laura Filonzi ◽  
Paola Palanza ◽  
Annalisa Pelosi ◽  
...  
Keyword(s):  
Salivary Cortisol ◽  
Physiological Stress ◽  
Conclusive Evidence ◽  
Harm Avoidance ◽  
Phenotypic Traits ◽  
Sport Competition ◽  
Physiological Stress Response ◽  
Before And After ◽  
Cortisol Levels ◽  
S Allele

Abstract Objectives There is evidence suggesting that in martial arts competitions athletes characterized by higher anxiety and harm avoidance may be more likely to lose a fight. This psychological profile has been hypothesized to explain in part the observation that cortisol is higher in losers before and in response to a competition. An important research target that needs further exploration is the identification of phenotypic traits that can be helpful in predicting athletes’ performance. Here we present a brief description of the theoretical bases that drives our research in the evolutionary psychobiology of sports and illustrate preliminary data on the relationship between the 5HTTLPR genotype, salivary cortisol, temperament and competition. Methods Sixty-five healthy male non-professional athletes provided saliva samples 10 min before and after a kumite session and filled out the Tridimensional Personality Questionnaire. Results Salivary cortisol levels 10 min before the competition were higher in losers and in athletes with the S allele. Temperament was associated with competition outcome and cortisol: losers were characterized by higher scores of harm avoidance and harm avoidance was positively correlated with cortisol levels. Conclusions The results confirm previous findings linking temperamental traits, pre-and post- competition physiological stress response with competition outcome in kumite fight. Moreover, they indicate an association between the 5HTTLPR polymorphism and pre-competition salivary cortisol, thus providing a preliminary but non-conclusive evidence on the role played by the 5HTTLPR genotype as a vulnerability factor in sport competition.

scholarly journals Effects of Litsea cubeba (Lour.) Persoon Essential Oil Aromatherapy on Mood States and Salivary Cortisol Levels in Healthy Volunteers

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Chaiyavat Chaiyasut ◽  
Bhagavathi Sundaram Sivamaruthi ◽  
Jakkrit Wongwan ◽  
Kanyarat Thiwan ◽  
Wandee Rungseevijitprapa ◽  
...  
Keyword(s):  
Essential Oil ◽  
Healthy Volunteers ◽  
Cortisol Level ◽  
Salivary Cortisol ◽  
Mood States ◽  
Healthy Human ◽  
Litsea Cubeba ◽  
Salivary Cortisol Level ◽  
Cortisol Levels ◽  
The Impact

Aromatherapy is one of the complementary therapies to improve health. The aromatic essential oils have been used in the treatment procedure through inhalation of essential oil vapor, massage, and herbal bathing. Litsea species are generally used in traditional medicine, and Litsea cubeba (Lour.) Persoon is a potent fumigant plant, used in cosmetics and foods as essence. The chemical composition of the essential oil of different parts of L. cubeba has been found to be varied. L. cubeba essential oil (LEO) is known for the treatment of cognition-associated discomforts. The current study assessed the impact of inhalation of LEO on mood states and salivary cortisol levels of healthy people. Fifteen healthy volunteers were involved in the study. The Profile of Mood States (POMS) Questionnaire and ELISA methods were employed to determine the mood states and salivary cortisol level, respectively. (-)-β-pinene, β-citral, cis- and trans-citral, citronellal, limonene, linalool, and 6-methyl-5-hepten-2-one were detected in LEO by GC-MS analysis. The heart rate and blood pressure were not affected significantly during LEO exposure. The inhalation of LEO significantly improved the total mood disturbance and reduced the confusion among the healthy human subjects. LEO inhalation reduced the salivary cortisol level at a notable level. The results of the current study warrant further studies on the beneficial effect of LEO aromatherapy in healthy and diseased subjects to uncover the therapeutic nature of the L. cubeba plant.

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