scholarly journals The applicability of 10 ml cryotubes for sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus)

2018 ◽  
pp. 93-97
Author(s):  
Levente Várkonyi ◽  
Zoltán Bokor ◽  
Árpád Ferincz ◽  
Ádám Staszny ◽  
Ferenc Fodor ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Sperm Cryopreservation ◽  
Freezing Method ◽  
Cyprinus Carpio Carpio ◽  
Significant Difference ◽  
Negative Effect

In our study, the comparison of 5 ml straw and 10 ml cryotube during sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus) was carried out. Three different dilution ratios (1:1, 1:4 and 1:9) were also tested using the cryotube. A significantly higher pMOT was recorded using the cryotube in comparison with the straw. VCL and STR were similar in both groups. Cryopreservation had a negative effect on pMOT and VCl using the cryotube and also the straw where, STR was not reduced significantly. An increasing tendency was observed using higher dilution of sperm during cryopreservation; however, significant difference was not recorded between the three groups. VCL and STR were similar in all groups. Cryotube was applicable for the sperm cryopreservation of the Hungarian carp landrace. However, the standardization for the freezing method specified for this volume is recommended. The different dilution ratios needed be tested also during fertilization.

scholarly journals DMSO and sucrose combination as a cryoprotectant of Najawa carp (Cyprinus carpio L.) sperm cryopreservation

2020 ◽  
Vol 147 ◽  
pp. 01014
Author(s):  
Lenggang Mamitu Krisdahasti ◽  
Ignatius Hardaningsih ◽  
Dini Wahyu Kartika Sari
Keyword(s):  
Dimethyl Sulfoxide ◽  
Cyprinus Carpio ◽  
Sperm Cryopreservation ◽  
Progressive Motility ◽  
Randomized Design ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Completely Randomized Design

This study was designed to evaluate a DMSO (Dimethyl Sulfoxide) and sucrose combination as a cryoprotectant for cryopreservation of Najawa carp (Cyprinus carpio L.) sperm. The experiment was conducted according to the Completely Randomized Design (CRD) with four treatments 10% DMSO + 0.2 M sucrose, 10% DMSO + 0.1 M sucrose, 5% DMSO + 0.2 M sucrose, 5% DMSO + 0.1 M sucrose. Each treatment consisted of three replications. The parameter observed were sperm progressive motility of fresh sperm, diluted sperm before freezing, and diluted sperm after freezing 1 day; 7 days; 14 days; 21 days; 28 days. The data were analyzed by ANOVA. The result showed that there was no significant difference between treatments (Sig. > 0.05). The highest relative protection was 89.51% carried out by 5% DMSO + 0.1 M sucrose.

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

Pengaruh Luka Iris Pada Tikus Dengan Paparan Hidrogen Peroxida 3% terhadap Epitelisasi dan Pembentukan Eksudat pada Permukaan Epitel Kulit.

2020 ◽  
Vol 17 (2) ◽  
pp. 172
Author(s):  
HARMAN AGUSAPUTRA ◽  
MARIA SUGENG ◽  
AYLY SOEKAMTO ◽  
ATIK WULANDARI
Keyword(s):  
Hydrogen Peroxide ◽  
Wound Healing ◽  
The Other ◽  
Delayed Wound Healing ◽  
Peroxide Group ◽  
Hemostatic Effect ◽  
Significant Difference ◽  
Negative Effect ◽  
Wound Healing Effect ◽  
Epithelial Growth

<p><strong>Abstract</strong></p><p><strong>Background:</strong> Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as antiseptic has been used frequently to clean woundsin in hospitals and clinics. Hydrogen peroxide has the effectof strong oxidative that can kill pathogens. It can clean up debris and necrotic tissuesin wounds. Hydrogen peroxidealso has hemostatic effect that can help to stop bleeding. Besides antiseptic effects, hydrogen peroxide i s suspected of having negative effect in wound healing. Hydrogen peroxide presumably could cause delayed wound healing by exudate formation and delayed epithelial growth.</p><p><strong>Method</strong>: This study was conducted in the laboratory using 48 white mice that were divided into 2 groups. All the mice were purposely wounded. Afterwards in one group the wounds were clean up using hydrogen peroxide, while in the other group without hydrogen peroxide as control. The wounds of both groups were observed on day 1, day 3 and day 7. On day 1 and day 3, both groups did not show significant difference.</p><p><strong>R</strong><strong>esult</strong> : on day 7 showed that the wound healing in hydrogen peroxide group were delayed. Fifty percent of them had the formation of exudate and 62.5% of them showed delayed epithelial growth.</p><p><strong>Conclusion </strong>: This study could show hydrogen peroxide as wound antiseptic has delayed wound healing effect.</p><p><strong>Keyword</strong>: hydrogen peroxide, wound healing</p>

scholarly journals Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage

2020 ◽  
Vol 147 ◽  
pp. 01015
Author(s):  
Dimas Fendy Pradana ◽  
Ignatius Hardaningsih ◽  
Dini Wahyu Kartika Sari
Keyword(s):  
Low Temperature ◽  
Cyprinus Carpio ◽  
Sperm Viability ◽  
Low Temperature Storage ◽  
Progressive Motility ◽  
C Storage ◽  
Randomized Design ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Temperature Storage

The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose.

Baerveldt shunt surgery versus combined Baerveldt shunt and phacoemulsification: a prospective comparative study

2017 ◽  
Vol 102 (9) ◽  
pp. 1248-1253 ◽  
Author(s):  
Mohamad El Wardani ◽  
Ciara Bergin ◽  
Kenza Bradly ◽  
Eamon Sharkawi
Keyword(s):  
Complication Rates ◽  
Failure Rates ◽  
Shunt Surgery ◽  
Patient Demographics ◽  
Aqueous Shunt ◽  
Included Patient ◽  
Significant Difference ◽  
Negative Effect ◽  
Prospective Comparative Study ◽  
Baerveldt Tube

AimTo examine the efficacy and safety of Baerveldt tube (BT) implantation compared with combined phacoemulsification and Baerveldt tube (PBT) implantation in patients with refractory glaucoma.MethodsSeventy-six eyes of 76 patients were enrolled, 38 pseudophakic eyes underwent BT implantation alone and 38 phakic eyes underwent the BT implantation combined with phacoemulsification. Groups were matched for preoperative intraocular pressure (IOP) and number of glaucoma medications. Preoperative and postoperative measures recorded included patient demographics, visual acuity (VA), IOP, number of antiglaucoma medications and all complications. Patients were followed up for a minimum of 36 months. Failure was defined as: inadequate IOP control (IOP≤5 mm Hg/>21 mm Hg/<20% reduction from baseline, reoperation for glaucoma, loss of light perception vision, or removal of the implant).ResultsThere was a significant difference in failure rates between groups at 36 months (PBT 37% vs BT 15%, P=0.02). There was no significant difference for PBT versus BT in preoperative baseline ocular characteristics. At 36 months: median IOP=14 mm Hg vs 12 mm Hg, P=0.04; mean number of antiglaucomatous medications=1.7 vs 1.3, P=0.61; median VA=0.8 vs 0.7, P=0.44. Postoperative complication rates were similar in both groups (n=5 vs 5; 13% vs 13%).ConclusionsFailure rates were significantly greater in the PBT group at 3 years. Median IOP was also significantly higher in the PBT group. These results suggest that combining phacoemulsification with aqueous shunt surgery may have a negative effect on long-term shunt bleb survival.

Impact of the Content Characteristic of Short Message Service Advertising on Consumer Attitudes

2014 ◽  
Vol 42 (9) ◽  
pp. 1409-1419 ◽  
Author(s):  
Hongyan Lin ◽  
Xing Zhou ◽  
Zhankui Chen
Keyword(s):  
Structural Equation ◽  
Short Message Service ◽  
Consumer Attitudes ◽  
Equation Modeling ◽  
Short Message ◽  
Mediating Role ◽  
Significant Difference ◽  
Message Service ◽  
Attitudes Toward Advertising ◽  
Negative Effect

We examined the influence of the overall content characteristic of short message service (SMS) advertising on consumer attitudes toward advertising on mobile devices. Attitudes of 246 Chinese (145 women, 101 men) toward permission-based SMS advertising received on electronic products were measured using structural equation modeling. Results showed that of the 4 dimensions of the overall content characteristic, entertainment, informativeness, and credibility positively and significantly influenced the attitude of consumers, whereas irritation had a negative effect. In addition, the overall content characteristic significantly impacted on consumer attitudes, with advertising value playing a mediating role. Finally, the influencing path coefficients revealed a significant difference according to gender. The credibility dimension had a greater impact on attitude toward the overall content characteristic for women than for men. Theoretical and practical implications are discussed.

scholarly journals Effect of the diet with commercial dry yeast (Saccharomyces cerevisiae) on organoleptic qualities, chemical and biological parameters of common carp (Cyprinus carpio L.)

2019 ◽  
Vol 11 (1) ◽  
pp. 84-89
Author(s):  
N.M. Abdulrahman ◽  
I.H. Al-Refaiee ◽  
H. Ali Mutter
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fish Flesh ◽  
Common Carp Cyprinus Carpio ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Experimental Treatments ◽  
Different Levels

Abstract. The purpose of the present study was to evaluate the replacement of different levels of animal protein concentrate (APC) with a commercial dry yeast Saccharomyces cerevisiae in diets on common carp performance. The experiment was conducted in the fish laboratory of the Department of Animal Production, College of Agricultural Sciences, University of Sulaimani in Kurdistan region of Iraq for the period from 25.07.2015 to 15.10.2015. Starting with a period of acclimatization for 21 days, to test the efficiency of using commercial dry yeast S. cerevisiae as alternative protein source to APC used in the manufacturing of diets for common carp (Cyprinus carpio L.) by using 90 fish at weights ranged 22-42g divided into 15 groups distributed randomly on 15 plastic containers by five treatments with three replicates per each variant. The treatments contain different levels of APC and yeast S. cerevisiae as follows: first treatment (Control T1): 100% APC / 0.00% yeast S. cerevisiae; second treatment (T2): 75% APC / 25% yeast S. cerevisiae; third treatment (T3): 50% APC / 50% yeast S. cerevisiae; fourth treatment (T4): 25% APC / 75% yeast S. cerevisiae and fifth treatment (T5): 0.00% APC / 100% yeast S. cerevisiae. There was no significant difference observed in the value of biological indices for some physiological organs, spleen and Hepatic pancreases and also in the value of the condition factor (CF) between carps from different treatments. The results of the chemical composition of the fish flesh showed significant difference in the moisture of individuals from T4 as compared with these from T2 and T5, T2 was significantly increased in crude protein as compared with other treatments, T5 had significant differences in fat crude as compared with other treatments, T1 and T2 were significantly different in ash as compared with other treatments, T1 was significantly different in carbohydrates as compared with other treatments. The results showed no significant differences observed among experimental treatments in Panel test of tenderness, color, juiciness, flavor and overall acceptance for fish meat.

scholarly journals ANALISIS PERBEDAAN RASIO KEUANGAN BANK UMUM KONVESIONAL DAN BANK UMUM SYARIAH SERTA PENGARUHNYA TERHADAP PENYALURAN KREDIT ATAU PEMBIAYAAN (Studi empiris yang tedrdaftar di BEI periode 2013-2016)

2017 ◽  
Vol 5 (1) ◽  
pp. 9-23
Author(s):  
Dika Ismawati ◽  
Edy Supriyono
Keyword(s):  
Commercial Bank ◽  
Commercial Banks ◽  
Secondary Data ◽  
Capital Adequacy ◽  
Financial Ratios ◽  
Operating Efficiency ◽  
Analysis Technique ◽  
Significant Difference ◽  
Negative Effect ◽  
General Data

This study aims to obtain evidence of differences in financial ratios of conventional commercial banks and sharia commercial banks, as well as the influence of financial ratios, liquidity, capital adequacy, problem loans, profitability, operating efficiency of lending. This study uses secondary data. The sample in this research is conventional commercial bank and sharia public bank listed on BEI with 4 year observation period (2013-2016). This sample was chosen by purposive sampling method. Independent sample t-test is used to test whether there is difference of average of two interconnected samples, multiple linear regression is used as data analysis technique as many as 148 general data of banking company, conventional bank and syariah commercial bank as many as 44 company data period 2013-2016 . The results of this study indicate that there are differences in average liquidity, problem loans, profitability, operating efficiency, While the average capital adequacy there is no significant difference between conventional commercial banks and sharia banks. While liquidity and profitability have a positive effect on lending, non performing loans and operating efficiency have a negative effect on lending, and capital adequacy does not affect credit disbursement.  

scholarly journals The Effect of Abiotic Stresses on the Protein Composition of Four Hungarian Wheat Varieties

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
Dalma Nagy-Réder ◽  
Zsófia Birinyi ◽  
Marianna Rakszegi ◽  
Ferenc Békés ◽  
Gyöngyvér Gell
Keyword(s):  
Abiotic Stress ◽  
Protein Content ◽  
Functional Properties ◽  
Crop Production ◽  
Global Climate ◽  
Protein Composition ◽  
Grain Protein Content ◽  
Wheat Varieties ◽  
Significant Difference ◽  
Negative Effect

Global climate change in recent years has resulted in extreme heat and drought events that significantly influence crop production and endanger food security. Such abiotic stress during the growing season has a negative effect on yield as well as on the functional properties of wheat grain protein content and composition. This reduces the value of grain, as these factors significantly reduce end-use quality. In this study, four Hungarian bread wheat cultivars (Triticum aestivum ssp. aestivum) with different drought and heat tolerance were examined. Changes in the size- and hydrophobicity-based distribution of the total proteins of the samples have been monitored by SE- and RP-HPLC, respectively, together with parallel investigations of changes in the amounts of the R5 and G12 antibodies related to celiac disease immunoreactive peptides. Significant difference in yield, protein content and composition have been observed in each cultivar, altering the amounts of CD-related gliadin, as well as the protein parameters directly related to techno-functional properties (Glu/Gli ratio, UPP%). The extent of changes largely depended on the timing of the abiotic stress. The severity of the negative effect depended on the growth stage in which abiotic stress occurred.

scholarly journals Aberrant Lighting Causes Anxiety-like Behavior in Mice but Curcumin Ameliorates the Symptoms

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2590
Author(s):  
Dhondup Namgyal ◽  
Kumari Chandan ◽  
Sher Ali ◽  
Ajaz Ahmad ◽  
Maha J. Hashim ◽  
...  
Keyword(s):  
Body Weight ◽  
Research Field ◽  
The Body ◽  
Laboratory Animals ◽  
Artificial Lighting ◽  
Lighting Conditions ◽  
Daily Food ◽  
Significant Difference ◽  
Negative Effect ◽  
Lighting Systems

In the modern research field, laboratory animals are constantly kept under artificial lighting conditions. However, recent studies have shown the effect of artificial light on animal behavior and metabolism. In the present study on mice, following three weeks of housing in dim light at night (dLAN; 5lux) and complete darkness (DD; 0lux), we monitored the effect on body weight, daily food intake, anxiety-like behavior by employing the open field test, and expression of the period (PER1) gene. We also studied the effect of oral administration of different concentrations of curcumin (50, 100, and 150 mg/kg) for three weeks in the same mice and monitored these parameters. The exposure to dLAN had significantly increased the anxiety-like behavior and body weight possibly through the altered metabolism in mice, whereas exposure to DD caused increased anxiety but no significant difference in weight gain. Moreover, the expression of the PER1 gene involved in sleep was also found to be decreased in the aberrant light conditions (dLAN and DD). Although the treatment of curcumin had no effect on body weight, it ameliorated the anxiety-like behavior possibly by modulating the expression of the PER1 gene. Thus, alteration in the light/dark cycle had a negative effect on laboratory animals on the body weight and emotions of animals. The present study identifies the risk factors associated with artificial lighting systems on the behavior of laboratory animals and the ameliorative effects of curcumin, with a focus on anxiety-like behavior.

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