scholarly journals Microhardness and Scanning Electron Microscopic Morphology of Permanent Dentin Following the Application of Iranian and Foreign-Made Desensitizing Toothpastes

2020 ◽  
Vol 12 (3) ◽  
pp. 86-92
Author(s):  
Azam Valian ◽  
Mina Jafari ◽  
Hosna Ebrahimizadeh
Keyword(s):  
Repeated Measures ◽  
Negative Control ◽  
Superior Performance ◽  
Control Group ◽  
Electron Microscopic ◽  
Significant Difference ◽  
The Mean ◽  
Microscopic Morphology ◽  
Scanning Electron

Background: This study aimed to assess the microhardness and morphology of permanent dentin following the application of Iranian and foreign-made desensitizing toothpastes. Methods: This in vitro, experimental study evaluated 48 dentin samples prepared of the extracted sound human permanent molars. Dentin samples were randomly divided into three groups (n=16) and subjected to the application of Pooneh Iranian desensitizing toothpaste, Colgate Sensitive Pro- Relief (Poland), and no intervention (negative control). Each dentin sample was then immersed in 5 mL of a demineralizing solution for 10 hours and underwent a demineralization/remineralization cycle (pH cycling) for 14 days. The mean microhardness of the samples was measured at baseline after demineralization and after 14 days. One sample of each toothpaste group was selected for the scanning electron microscopy (SEM) assessment of dentin morphology. Finally, repeated-measures ANOVA was used to analyze the effect of time and type of toothpaste on microhardness using SPSS, version 21. Results: The comparison of the mean microhardness of the control group with experimental groups revealed no significant difference at baseline or after demineralization (P > 0.05) although this difference was significant after 14 days (P < 0.001). The mean microhardness of the two toothpaste groups was not significantly different at different time points (P > 0.05). Finally, the SEM assessment revealed a greater tubular obstruction in Pooneh group after 14 days. Conclusions: Pooneh Iranian-made desensitizing toothpaste was comparable to Colgate Sensitive Pro-Relief foreign-made desensitizing toothpaste in terms of the microhardness of permanent dentin. It even demonstrated superior performance with regard to the obstruction of dentinal tubules.

scholarly journals In vitro comparison of the cytotoxicity of different endodontic sealers; AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal

2021 ◽  
Author(s):  
Mehdi Dastorani ◽  
Muhammad javad Aliee ◽  
Raheleh Halabian ◽  
Mostafa Solati ◽  
Mohammadsadegh Alemrajabi
Keyword(s):  
Repeated Measures ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Periapical Lesions ◽  
Ah Plus ◽  
Low Cytotoxicity ◽  
Endodontic Sealers

Abstract Background: This study aimed to assess the cytotoxicity of four commonly used endodontic sealers namely AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal against human gingival fibroblasts (HGFs). Methods: After culturing the HGFs, they were exposed to the respective sealers in set form and in five different weights, after sterilization. The cytotoxicity of the sealers was evaluated after 1, 3 and 7 days using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by repeated measures ANOVA. Results: After 24 h, all sealers showed low cytotoxicity. However, all sealers in 250 mg and 500 mg weights showed significantly higher cytotoxicity than the negative control group at 72 h, and 7 days (P<0.05) except for AdSeal in 80 mg weight (P>0.05). AH Plus was significantly more cytotoxic than other sealers at 3 and 7 days (P<0.05) while AdSeal had the closest results to the negative control group, and showed significantly higher biocompatibility than other sealers in 250 mg concentration. Conclusion: AdSeal showed the highest biocompatibility while AH Plus had the highest cytotoxicity among the tested sealers. Thus, its application may delay the healing of periapical lesions.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

scholarly journals THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO

2018 ◽  
Vol 11 (12) ◽  
pp. 411
Author(s):  
Ameta Primasari ◽  
Minasari Nasution ◽  
Nurul Hidayati Arbi ◽  
Dini Permata Sari ◽  
Mohammad Basyuni
Keyword(s):  
Leaf Extract ◽  
Brain Heart Infusion ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Negative Control ◽  
Plate Count ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Mueller Hinton Agar

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.

scholarly journals Antibacterial Activity of Lumbricus Rubellus Earthworm Extract Against Porphyromonas Gingivalis as the Bacterial Cause of Periodontitis

2019 ◽  
Vol 7 (6) ◽  
pp. 1032-1036 ◽  
Author(s):  
I Gusti Agung Ayu Dharmawati ◽  
Tjokorda Gde Bagus Mahadewa ◽  
I Putu Eka Widyadharma
Keyword(s):  
Antibacterial Activity ◽  
Treatment Group ◽  
Lumbricus Rubellus ◽  
Control Group ◽  
Inhibitory Zone ◽  
Control Group Design ◽  
Zone Diameter ◽  
Significant Difference ◽  
The Mean

AIM: The purpose of this study was to determine the antibacterial activity of Lumbricus rubellus earthworms through inhibitory zone diameter to the growth of the bacterium Phorphyromonas gingivalis as the cause of periodontitis. METHODS: This was an experimental study with randomised posttest-only control group design. The study was conducted at the Microbiology Research Center laboratory at the Faculty of Dentistry, Airlangga University, Indonesia. The study was conducted in vitro, the sample size was calculated using the Federer formula as many as four agar plates containing bacteria Phorphyromonas gingivalis, with each plate given five different treatments: control (ethanol), Lumbricus rubellus earthworm extract (ECT) with concentrations of 50%, 25%, 12.5%, and 6.25% respectively. The data in the form of inhibition zone diameter (measured in millimetres) obtained were tested using One-Way ANOVA. RESULTS: The mean diameter of the inhibitory zone extract of Lumbricus rubellus earthworm on the growth of Phorphyromonas gingivalis bacteria in the treatment group had significant differences (p < 0.05). The mean inhibition zones between controls and the ECT treatment group (ECT 50%, ECT 25%, ECT 12.5%) were statistically different (p < 0.05), in contrast with ECT 6.25% (p > 0.05) which did not show significant difference with the control group (p > 0.05). CONCLUSION: Lumbricus rubellus earthworm extract with a concentration of 50% has the largest diameter of the inhibitory zone on the growth of the Phorphyromonas gingivalis bacteria. The 6.25% earthworm extract showed no antibacterial activity against the growth of Phorphyromonas gingivalis bacteria.

scholarly journals In Vitro Evaluation of the Efficacy of Different Over-the-Counter Products on Tooth Whitening

2015 ◽  
Vol 26 (4) ◽  
pp. 373-377 ◽  
Author(s):  
Muhammet Karadas ◽  
Zeynep Yesil Duymus
Keyword(s):  
Repeated Measures ◽  
Positive Control ◽  
Negative Control ◽  
Carbamide Peroxide ◽  
Tooth Whitening ◽  
Over The Counter ◽  
Color Changes ◽  
Significant Difference ◽  
Cielab System

<p>This <italic>in vitro</italic> study evaluated the whitening effect of four different over-the-counter products compared with a 10% carbamide peroxide (CP) bleaching gel. Specimens obtained from bovine incisors were stained in a tea solution and randomly divided into six groups according to the product used (n=10): CT: conventional toothpaste (negative control); CWT: Crest 3D White toothpaste; CWR: Crest 3D White mouth rinse; CWS: Crest 3D White strips; DW: Dazzling White (paint-on gel); and OP: Opalescence PF 10% CP (at-home bleaching gel, positive control). Over-the-counter products and 10% CP were used according to the manufacturer's recommendations. Color measurements were performed according to the CIELab system using spectrophotometer after staining, and after 4 and 8 weeks of the treatment procedure. The ∆E, ∆L, ∆a, and ∆b values were calculated. The data obtained were analyzed by repeated-measures ANOVA. Tukey's test for multiple comparisons was applied (p<0.05). All groups, except CWT, were effective in tooth whitening in comparison with conventional toothpaste. There was no significant difference in tooth whitening between CWT and CT (p=0.93). CWS, DW and OP groups presented significantly higher color changes than the CWR group. DW and CWS showed similar tooth whitening to OP.</p>

In vitro Comparative Evaluation of the Fracture Resistance of Simulated Immature Teeth reinforced with Different Apical Barriers and Obturation Combination

2016 ◽  
Vol 7 (3) ◽  
pp. 113-118
Author(s):  
Neveen A Shaheen ◽  
Nahla G El-Din El-Helbawy
Keyword(s):  
Fracture Resistance ◽  
Comparative Evaluation ◽  
Negative Control ◽  
Control Group ◽  
Root Canals ◽  
Immature Teeth ◽  
Gutta Percha ◽  
Group I ◽  
Significant Difference

ABSTRACT Aim This study aimed to assess and compare the fracture resistance of simulated immature teeth reinforced with Biodentine (BD) and mineral trioxide aggregate (MTA) as apical barriers and two root canal backfilling combination (gutta-percha/AH26, MetaSeal). Materials and methods A total of 70 extracted human maxillary incisors were randomly divided into seven groups (n = 10). The positive control group was not instrumented. For the other groups, coronal access was made and root canals were instrumented using the ProTaper, up to F5 followed by six Peeso reamers which were allowed to pass 1 mm beyond the apex to size 6 (1.7 mm) to simulate immature teeth. The apical 4 mm of their root canals was filled with either MTA or BD apical barrier, then backfilled with gutta-percha/AH26 or MetaSeal obturation combination. The negative control group was left unfilled. Composite resin was used to restore the coronal access cavities. The maximum load for fracture of each tooth was recorded utilizing a universal testing machine. Data were analyzed using two-way analysis of variance. Results The noninstrumented group I had the highest fracture resistance and differed significantly (p < 0.05) from the negative control groups. On the contrary, no significant difference was found between BD and MTA groups, regardless of the backfilling combination (p > 0.05). Conclusion There was no difference between MTA and BD apical barriers and the backfilling combination regarding their resistance to root fracture. How to cite this article Shaheen NA, El-Din El-Helbawy NG. In vitro Comparative Evaluation of the Fracture Resistance of Simulated Immature Teeth reinforced with Different Apical Barriers and Obturation Combination. World J Dent 2016;7(3):113-118.

scholarly journals Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Saeedeh Galledar ◽  
Robab Farhang ◽  
Malek Abazari ◽  
Parisa Negahdar
Keyword(s):  
Root Canal ◽  
Test Group ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: Proper apical seal plays an important role in the success of root canal treatment. The most common cause of failure of root canal therapy is known as the lack of adequate apical seal. The aim of this in vitro study was to compare the apical microleakage of MTA Fillapex, , and Endofill sealers using dye penetration method. Material and Methods: In this in vitro study, 72 single-rooted extracted human teeth were selected. The teeth were randomly divided into three experimental groups of 20 and two positive and negative control groups of 6. The canals were prepared by step-back technique and then filled with gutta-percha and one of the sealers mentioned. In the positive control group, the canals were filled with gutta-percha without sealer, and in the negative control group, the canals were prepared but not filled. The teeth were immersed in 2% methylene blue dye for 72 hours. The teeth were then cut longitudinally and the level of dye penetration was measured under a stereomicroscope. Data were analyzed by SPSS ver. 19 software, ANOVA and Bonferroni post-hoc tests. Results: The mean level of dye penetration in the Endofill test group was significantly higher than that in the  and MTA Fillapex test groups. While, the observed difference between  and MTA Fillapex groups was not statistically significant (p<0.05). Conclusion: The results of this study showed that  and MTA Fillapex sealers did not show any significant difference in apical seal properties. However, their sealing strength was significantly greater than Endofill sealer.KeywordsAH26 sealer; Endofill; MTA Fillapex; Microleakag

scholarly journals Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

2020 ◽  
Author(s):  
Manisha Nepal ◽  
Snigdha Shubham ◽  
Rupam Tripathi ◽  
Jwolan Khadka ◽  
Deepa Kunwar ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Filling Materials ◽  
Diamond Bur ◽  
Retrograde Filling ◽  
Significant Difference ◽  
Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs. Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm. Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively. Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

scholarly journals Influence of Simulated Pulpal Pressure on Efficacy of Bleaching Gels

2014 ◽  
Vol 15 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Carlos Rocha Gomes Torres ◽  
Graziela Ribeiro Batista ◽  
Alessandra Bühler Borges ◽  
Paula Tamião Arantes ◽  
Annette Wiegand ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Artificial Saliva ◽  
Negative Control ◽  
Color Variation ◽  
Color Measurement ◽  
Control Group ◽  
Significant Difference ◽  
Bleaching Gels ◽  
Bleaching Treatment

ABSTRACT Aim The aim of this study was to investigate the influence of simulated pulpal pressure on efficacy of bleaching gels. Materials and methods Cylindrical enamel-dentin specimens from bovine teeth (3 mm diameter, enamel and dentin layer each 1 mm thick) were divided into 4 groups, according to the bleaching treatment: negative control (non-bleached), bleached with 10% carbamide peroxide (CP), bleached with 7.5% hydrogen peroxide (HP) and bleached with 35% hydrogen peroxide. Ten percent CP gel was applied for 8 h/day and 7.5% HP for 1 h/day, during 14 days. For 35% HP treatment, two sessions of 45 minutes each were employed. In intermediate periods specimens were stored in artificial saliva. Experimental groups (n = 19) were subdivided according to the simulation of pulpal pressure (25 mm Hg) during bleaching treatment. Initial color measurement and after bleaching treatment were assessed by spectrophotometry, using CIE L*a*b* system. The data were statistically analyzed by ANOVA and Dunnett's posthoc tests (p < 0.05). Results There was significant difference of ∆E for all experimental groups compared to negative control group, according to Dunnett's test (p < 0.0001). There were no significant difference for total color variation (∆E) among experimental groups (p > 0.05). Conclusion It was concluded that all bleaching gels showed bleaching efficacy compared to non-bleached group and that the simulated pulpal pressure did not influence the bleaching outcomes of the tested gels. Clinical significance Although numerous in vitro studies investigating the efficacy of bleaching agents have been performed, they do not properly simulate the pulpal pressure. In order to make these studies closer to clinical conditions, it is important to reproduce these conditions in laboratory, so the results can be more reliable. This in vitro study was performed under simulated pulpal pressure, aiming to investigate its influence on dental bleaching outcomes. How to cite this article Borges AB, Batista GR, Arantes PT, Wiegand A, Attin T, Torres CRG. Influence of Simulated Pulpal Pressure on Efficacy of Bleaching Gels. J Contemp Dent Pract 2014;15(4):407-412.

scholarly journals Comparison of MTA, CEM Cement, and Biodentine as Coronal Plug during Internal Bleaching: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Paridokht Zarean ◽  
Parichehr Zarean ◽  
Arash Ravaghi ◽  
Maryam Zare Jahromi ◽  
Mitra Sadrameli
Keyword(s):  
Root Resorption ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Cem Cement ◽  
Significant Difference

Background. Internal bleaching is a choice of treatment in discolored endodontically treated teeth. Cervical root resorption is one of the important complications of this treatment. A suggested procedure to prevent this type of resorption is using a coronal barrier under the bleaching materials. The aim of the study was to compare the microleakage of mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) cement, and Biodentine. Materials and Methods. In this in vitro study, a total of 60 single canal incisors were included. They were randomly divided into three experimental groups (n = 16), one positive control group (n = 6), and one negative control group (n = 6). Coronal portion of the canals in the experimental groups was sealed with 3 mm of MTA, CEM cement, or Biodentine as a coronal barrier. After 3 days, specimens were bleached. A fresh Enterococcus faecalis suspension was added to the samples. The culture tubes were observed for 45 days, and the daily turbidity was recorded. Statistical analysis was accomplished by the Kaplan–Meier test and SPSS 22. Results. All positive samples showed turbidity, whereas none of the negative samples allowed bacterial leakage. Results showed no significant difference between MTA, CEM cement, and Biodentine groups. ( P value = 0.304, 0.695, and 0.217). The bacterial microleakage for the two groups also did not show significant differences. Conclusions. CEM cement and Biodentine showed promising results as coronal plug, and clinical studies are needed to test these materials with MTA for avoiding microleakage in internal bleaching treatment.

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