scholarly journals Bioactivities, physicochemical parameters and GC/MS profiling of the fixed oil of Cucumis melo L seeds: A focus on anti-inflammatory, immunomodulatory, and antimicrobial activities

2021 ◽  
Vol 10 (4) ◽  
pp. 476-485
Author(s):  
Amira A. El-anssary ◽  
Gehan Fawzy Abdel Raoof ◽  
Dalia Osama Saleh ◽  
Hossam Mohammed El-Masry
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Cucumis Melo ◽  
Physicochemical Parameters ◽  
Antimicrobial Activities ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Waste Products ◽  
Anti Inflammatory ◽  
Cucumis Melo L

Introduction: Recently, the recovery of waste products from plants as a source of biologically active compounds has increased interest. Therefore, the current research aims to evaluate the anti-inflammatory, immunomodulatory and antimicrobial activities of the fixed oil of Cucumis melo L seeds, as well as to investigate its physicochemical parameters and chemical composition. Methods: Anti-inflammatory activity was examined using carrageenan-induced rat paw edema assay. The antimicrobial activity was assayed against Staphylococcus aureus, Micrococcus luteus, Enterococcus faecalis, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans by well diffusion method. The chemical composition of the oil was determined by gas chromatography/mass spectrometry (GC/MS), α-tocopherol was estimated by high-performance liquid chromatography (HPLC). Results: Cucumis melo oil had no toxicity and possessed a promising anti-inflammatory activity. Moreover, the oil exhibited a reasonable decrease in the pro-inflammatory cytokines, including interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), and a significant increase in the anti-inflammatory cytokine (IL-10). The oil exhibited a reasonable antimicrobial activity against all tested organisms. The major identified compound in the unsaponifiable matter was (1-methyldodecyl) benzene (8.76%), while the major fatty acid was methyl linoleate (14.10%). The results of physicochemical characterization revealed the better quality of Cucumis melo oil. The amount of α-tocopherol in the oil was 23.5 μg/mL, which is considered a reasonable amount. Conclusion: These findings indicate that the fixed oil of Cucumis melo L seeds might be used as a safe natural anti-inflammatory, immunomodulatory and antimicrobial agent.

scholarly journals EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS

2017 ◽  
Vol 9 (5) ◽  
pp. 29
Author(s):  
Iswariya S. ◽  
Uma T. S.
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Aqueous Extract ◽  
Seed Extract ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Anti Inflammatory ◽  
Seed Extracts

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

Study of Anti-oxidant, Anti-inflammatory, Genotoxicity, and Antimicrobial Activities and Analysis of Different Constituents found in Rhizome Essential Oil of Curcuma caesia Roxb., Collected from North East India

2020 ◽  
Vol 21 (5) ◽  
pp. 403-413 ◽  
Author(s):  
Manabi Paw ◽  
Roktim Gogoi ◽  
Neelav Sarma ◽  
Sudin K. Pandey ◽  
Angana Borah ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Allium Cepa ◽  
Antimicrobial Activities ◽  
Commercial Application ◽  
Diffusion Method ◽  
Ms Analysis ◽  
Allium Cepa Assay ◽  
Anti Inflammatory ◽  
Mic Value

Background: This investigation was designed to evaluate the chemical composition, antioxidant, anti-inflammatory, genotoxicity, and antimicrobial activities of Curcuma caesia Roxb rhizome essential oil. Methods: Gas Chromatography/Mass Spectroscopy (GC/MS) analysis was performed to determine the chemical composition, standard antioxidative test DPPH assay, reducing power assay, in vitro antiinflammatory activity (egg albumin denaturation, protease inhibitory assay) by using standard methods. Similarly, antimicrobial activity was tested using the disc diffusion method, minimum inhibitory concentration ability (MIC); while to test genotoxicity, Allium cepa assay was used. Results: GC/MS analysis revealed eucalyptol (28.55%), epicurzerenone (19.62%), and camphor (21.73%) as the major components of C. caesia rhizome essential oil. Potent antioxidant (IC50= 48.08±0.003 μg/mL), anti-inflammatory (IC50= 121.7±0.0013 μg/mL), and antimicrobial activities of the essential oil were recorded better than the standard drugs Fluconazole for fungus and Ciprofloxacin for bacteria. The essential oil also possessed a strong antibacterial effect against two tested bacterial strains B. subtilis and B. cereus with 7.5 μg/mL MIC value, while for fungal strains the essential oil was most effective against S. cereviaceae with an MIC value of 2.5 μg/mL. All the data were recorded in triplicates. Allium cepa assay revealed minor genotoxicity with mitotic index, MI= 27.70%; chromosome aberration, A= 1.1% of C. caesia rhizome essential oil. Conclusion: C. caesia rhizome essential oil possesses potent antioxidant, anti-inflammatory, and antimicrobial properties with negligible genotoxicity. Hence, the present study is highly significant for the utilization of rhizome of C. caesia, a high-value ethnopharmacological plant for advanced R & D and commercial application.

Comparison of Chemical Composition and Antimicrobial Activities of the Essential Oil of Taxus Media and Taxus Chinensis Var. Mairei Leaves

2011 ◽  
Vol 343-344 ◽  
pp. 1092-1097 ◽  
Author(s):  
Jing Zhang ◽  
Ke Yuan ◽  
Yong Chun Jin
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Taxus Chinensis ◽  
Filter Paper Disc ◽  
Taxus Media ◽  
The Difference

The study was conceived to evaluate the difference in the chemical composition as well as the difference in the antimicrobial activities of the essential oils of Taxus media and Taxus chinensis var. mairei leaves. The chemical composition of essential oils which obtained by hydrodistillation method, were analysed by gas chromatography-mass spectrometry (GC-MS) system, and the relative amount of the chemical components of the essential oils were determined by the peak area normalization method. Remarkable differences were recorded between the percentages of a few constituents from the two essential oils. A total of 34 compounds were identified from the essential oil of Taxus media leaves, with the compounds of the dominant are Benzene propanenitrile (21.30%), 1, 4-Dioxane-2, 3-diol (20.13%), 3-Bromo-3-methyl-Butyric acid (17.92%) and 1-hydroxy-2-Butanone (9.85%). For the essential oil of Taxus chinensis var. Mairei leaves, 33 compounds were identified with the compounds of the dominant are Benzene propanenitrile (49.39%), 1-hydroxy-2-Butanone (12.72%), Acetic acid (5.39%), 1-octen-3-ol (4.28%) and (E)-3-(2-hydroxyphenyl)-2-Propenoic acid, (3.53 %). The filter paper disc agar diffusion method was used to test the antimicrobial activities of the essential oils, and diameter of antibacterial zones (DD) value and minimum inhibitory concentration (MIC) value were used to evaluate the antimicrobial activity. The result showed that the essential oil of Taxus media leaves possessed stronger antimicrobial activity than the essential oil of Taxus chinensis var. mairei leaves against the two tested bacteria.

scholarly journals Antimicrobial, Cytotoxic, and Anti-Inflammatory Activities of Pimenta dioica and Rosmarinus officinalis Essential Oils

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ana Cecilia Lorenzo-Leal ◽  
Enrique Palou ◽  
Aurelio López-Malo ◽  
Horacio Bach
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Ex Vivo ◽  
Antimicrobial Activities ◽  
Rosmarinus Officinalis ◽  
Inflammatory Activity ◽  
Inflammatory Agent ◽  
Anti Inflammatory ◽  
Pimenta Dioica ◽  
Anti Inflammatory Cytokine

Essential oils (EOs) are natural products composed of a mixture of volatile and aromatic compounds extracted from different parts of plants that have shown antimicrobial activities against pathogens. In this study, EOs extracted from Pimenta dioica (Myrtaceae) and Rosmarinus officinalis (Lamiaceae) were assessed for their antimicrobial activities using a panel of pathogenic Gram-positive, Gram-negative, and fungal strains. The antimicrobial activity was measured by the minimal inhibitory concentration required for the growth inhibition of the microorganisms. The cytotoxicity of the EOs was tested ex vivo using the model of human-derived macrophage THP-1 cells. In addition, an inflammatory response was evaluated using the anti-inflammatory cytokine IL-10 and the proinflammatory cytokines IL-6 and TNF-α. Results showed that both EOs had antimicrobial activity and different pathogens were exposed to concentrations ranging between 600 and 2000 μg/mL. In addition, the EOs showed no inflammatory activity when exposed to human macrophages, but a potent anti-inflammatory activity was measured when the oil from Rosmarinus officinalis was exposed to macrophages. This study demonstrates that the use of EOs is an effective alternative for pathogenic bacterial and fungal control, alone or in combination with antibiotic therapy. Moreover, the oil extracted from Rosmarinus officinalis could be used as potent anti-inflammatory agent.

scholarly journals Polyphenolic content, Antioxidant and Anti-inflammatory activities of Melon (Cucumis melo L. var. inodorus) Seeds

2020 ◽  
Vol 10 (2-s) ◽  
pp. 22-26
Author(s):  
Amel Bouaziz ◽  
Saliha Djidel ◽  
Assia Bentaher ◽  
Seddik Khennouf
Keyword(s):  
Antioxidant Activity ◽  
Cucumis Melo ◽  
Inflammatory Activity ◽  
Food Ingredients ◽  
Dry Extract ◽  
Anti Inflammatory ◽  
Cucumis Melo L ◽  
Promising Source ◽  
Β Carotene

The aim of this study was to estimate the content of polyphenols, flavonoids and tannins compounds and to evaluate the antioxidant activity and  the  in vitro anti-inflammatory  of    the ethanolic extract of  melon (Cucumis melo L.  var inodorus) seeds. The Folin-Ciocalteau and AlCl3 methods were applied in order to quantify the polyphenolic and flavonoids contents, respectively. However, DPPH and  β-carotene bleaching  method were applied  to evaluate the  in vitro antioxidant activity. The polyphenolic  and flavonoids contents  of    seeds  extract  were  found to be 37,10 ± 1,11 mg GAE/g of dry extract and 0,43 ± 0,27 mg QE/ g  of dry extract, respectively. Whereas the amount of tannins was  48, 30 ±  0,90 mg TAE/ g of dry extract. The scavenger effect of  seeds extract against DPPH radicals showed IC50 value of  4,13 ± 0,07 mg/ ml. The β-carotene bleaching assay indicated a strong inhibition percentage of the lipid peroxidation with value of 69,47 %. The in vitro anti-inflammatory activity was evaluated using the inhibition of   proteins denaturation revealed that the seeds extract had a moderate anti-inflammatory activity which is estimated at 49,09 % compared to the aspirin (97,73  %) at 1 mg/ ml.  Finally, melon seeds may be considered as a promising source of natural antioxidants which possess remarkable therapeutic action as inflammatory activity and may serve as food ingredients. Keywords: Cucumis melo var. inodorus, Polyphenols, Flavonoids, Tannins, Oxidative stress,  Anti-inflammatory activity.

scholarly journals CHEMICAL COMPOSITION AND ANTI-INFLAMMATORY ACTIVITY OFRoldana platanifolia

Química Nova ◽  
2015 ◽  
Author(s):  
Amira Arciniegas ◽  
Ana L. Pérez-Castorena ◽  
Antonio Nieto-Camacho ◽  
Jhon Ironzi Maldonado ◽  
José Luis Villaseñor ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Inflammatory Activity ◽  
Anti Inflammatory

Chemical composition and anti-inflammatory activity of a pectic polysaccharide isolated from sweet pepper using a simulated gastric medium

2011 ◽  
Vol 124 (1) ◽  
pp. 309-315 ◽  
Author(s):  
Sergey V. Popov ◽  
Raisa G. Ovodova ◽  
Victoria V. Golovchenko ◽  
Galina Yu. Popova ◽  
Feodor V. Viatyasev ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Sweet Pepper ◽  
Inflammatory Activity ◽  
Pectic Polysaccharide ◽  
Anti Inflammatory

scholarly journals Antibacterial, Antioxidant and Anti-Inflammatory Potential of the Different Extracts of Holoptelia Integrifolia

2019 ◽  
Vol 9 (2) ◽  
pp. 5234-5240
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Albino Rats ◽  
Nitric Oxide Radical ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

Medicinal plants are play significant impact in the personal medicine for most of the people all over the world as an alternative live saving medicines and most of their medicinal properties are well known for anticancer activity. The different extracts of Holoptelea integrifolia (H.integrifolia) leaves, stem bark and fruits were studied as a potent natural source of antimicrobial, antioxidant and wound healing potential. This work was carried out to evaluate antimicrobial, antioxidant and anti-inflammatory activity of different extracts of H.integrifolia. The antimicrobial activity of the H.integrifolia ethanolic extract was studied against five fungal and bacterial strains by utilizing the agar well diffusion method and MIC. Among several strain, the ethanolic extract of fruit has shown higher antimicrobial inhibition zone as 9.25-16 mm compare to other two extracts of stem and leaves as 10- 13.25 mm and 6-10.2 mm respectively. The antioxidant activities for different extract were also determined by DPPH free radical assay, Hydroxyl Radical Scavenging and Nitric Oxide Radical Scavenging Activity method. The anti-inflammatory activity also estimated basedon formalin induced paw edema method on Wistar albino rats. The different extracts of leaves, stem bark and fruit parts of Holoptelea integrifolia were estimated for in vivo anti-inflammatory activity against the animal model of female Wistar albino rats. The results of anti-inflammatory activity revealed that the Ethanol extracts showed vital and dose-dependent anti-inflammatory effects. Our findings revealed that aerial parts of H.integrifolia contais potential antimicrobial, antioxidant and anti-inflammatory compounds, which expose the medicinal potential of the selected plant could be a significant drug candidates against microbial, oxidative and inflammation-related pathological processes as a future alternative medicine.

Study of the chemical composition, antimicrobial activity of the essen- al oil of Cistus salviifolius from Tissemsilt National Park (Algeria) and influence of the drying period in the shade on the yield of this oil

2020 ◽  
Vol 9 (6) ◽  
pp. 238-244
Author(s):  
Meharrar Nadjet ◽  
Bekada Ahmed Mohamed Ali ◽  
Dris Ibrahim ◽  
Jacques Dommes ◽  
Arabi Abed ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Wide Spectrum ◽  
Diffusion Method ◽  
Bacterial Suspension ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Antibiotic Susceptibility Test ◽  
Cistus Salviifolius

The genera Cistus present groups of about twenty species of shrubs belon- ging to the Cistaceae family are very common throughout the Mediterranean region. Cistus salviifolius, known as Melliya is a species of this genus that occurs in the forest region of Mount Ouarsenis (northwest Algeria), exactly in Thniet El Had Na onal Park in the Wilaya of Tissemsilt. This study was un- dertaken to evaluate the an bacterial effects of C. salviifolius and its chemical composition in addition the effect of shade drying me on essen al oil yield in order to op mize this parameter which is most o en not controlled for producers. The op mal yield recorded is about 0.07± 0.02%. Its characte- riza on by gas chromatography coupled with mass spectrometry shows a dominance of hydrocarbon compounds. The minimum inhibitory concentration (MIC) is established using 6 strains of Gram-positive and negative bacteria. The antibiotic susceptibility test is performed using the giliose- environment diffusion method, a bacterial suspension is prepared and subsequently well homogenized. Opacity must be adjusted to 0.5 McF (McFarland), from a young exponen al culture (18 to 24 hours). The an mi- crobial ac vity of C. salviifolius EO was tested against three Gram-posi ve and three Gram-nega ve bacteria. In fact, a strong inhibitory and bactericidal effect has been demonstrated against all Gram-positive and Gram- negative bacteria tested. It appears from this study that the EO of C. salviifo- lius has a very high antimicrobial activity, it shows a wide spectrum covering Gram positive and Gram negative bacteria. Taking into account the results of this study, new an bacterial agents could be developed and the use of C.salviifolius should be encouraged in the pharmaceutical and perfumery industries.

Sign in / Sign up

Export Citation Format

Share Document