scholarly journals The effect of extract of Dunaliella salina L. on expression of anti-apoptotic BCL2 in Hela cell line

2021 ◽  
Vol 43 (2) ◽  
pp. 186-192
Author(s):  
Mahdie- Sadat Lajavardi ◽  
Mahsa Kavousi
Keyword(s):  
Gene Expression ◽  
Treatment Group ◽  
Hela Cell ◽  
Cell Line ◽  
Real Time ◽  
Real Time Pcr ◽  
Control Group ◽  
P Value ◽  
Hela Cell Line ◽  
Relative Expression

Background: After breast cancer, cervix neoplasm is the most common disease among young women. Nowadays, natural substances are used in the treatment of diseases, because of the known side effects of the chemical drugs. Dunaliella is a green alga that lives in the saltwater lakes of Iran and is abundant in antioxidant substances. This paper aimed to study the effect of Dunaliella extract on the expression of the anti-apoptotic BCL-2 gene in Hela cell line. Expression of this gene is increased during cancer. It is expected that gene expression will be reduced if the alga extract is effective. Methods: Hela was prepared from the Center for Genetic and Biological Reserves of Iran and cultured. After culture, cells were divided into two treatment and control groups. Different concentrations of algae extract were applied to the treatment group for 48 hours. Then its toxicity was measured using MTT assay and IC50 was determined. RNA was extracted from cells of two groups, to determine the relative amount of gene expression at a concentration of IC50. Then Real-time PCR was used. Results: The result of Real-time PCR showed that the relative expression of BCL-2, in treatment group cells that were affected by algae extract, was four times lower than the control group. Since the P-value is less than 0.05 (P-value = 0), this decrease is significant. Conclusion: After 48 hours, at a concentration of IC50 of algae extract, the relative expression of BCL-2 was four times lower than control group.

The Effect of deinoxanthin Isolated from Radioactivity-Resistant Bacteria on Expression of Bcl-2 Gene in Hela Cell Line

2021 ◽  
Vol 43 (1) ◽  
pp. 84-91
Author(s):  
Mahsa Kavousi ◽  
Hesam Bagheri
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Real Time ◽  
Real Time Pcr ◽  
Cellular Proliferation ◽  
Uterine Cancer ◽  
Inhibitory Effect ◽  
Resistant Bacteria ◽  
P Value ◽  
Hela Cell Line

Background: After breast cancer, uterine cervical neoplasms is the most common cancer in women. It is believed that genetic factors are effective in developing cancer. Bcl-2 is a well-known anti-apoptosis gene that increases cell viability without stimulating effect on cellular proliferation. Today it attempts to use natural compounds to control or treat diseases. Carotenoids are one of these compounds. Deinoxanthin is a carotenoid isolated from Deinococcus radiodurans. Since this bacterium has a unique ability to withstand radiation, and radiation is a well-known cause of cancer carotenoid synthesized by bacterium is worthwhile. The aim of study is evaluating the effect of deinoxanthin on the expression of Bcl-2 in Hela cell line. Methods: Active culture of bacteria was purchased from Genetic and Biological Reserve of Iran then deinoxanthin was extracted. Hela was prepared of Pasteur Institute of Iran and cultured. Cells were divided into two treatment and control groups. Deinoxanthin was affected on the treatment group and its toxicity was measured using MTT. Real-time ­PCR was used to measure gene expression. RNA was extracted from two groups and cDNA was made. Results: Real-time PCR showed the anti-apoptotic expression of Bcl-2 decreased by 4/85 and given that p-value of 0/05 was (p-value=0) this decrease is significant. Conclusion: Regarding the results of Real-time PCR it can be concluded deinoxanthin extract has an inhibitory effect on the uterine cancer cell line has an inhibitory effect after 48 hours and the amount of anti-apoptotic expression of Bcl-2 has significantly decreased (p-value=0).

scholarly journals CHANGES IN GENE EXPRESSION ASSOCIATED WITH NON-CANCER EFFECTS OF THE CHORNOBYL CLEAN-UP WORKERS IN THE REMOTE PERIOD AFTER EXPOSURE

2020 ◽  
Vol 25 ◽  
pp. 456-477
Author(s):  
I. Ilienko ◽  
◽  
D. Bazyka ◽  
N. Golyarnyk ◽  
L. Zvarych ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Main Group ◽  
Control Group ◽  
Chronic Obstructive ◽  
Relative Level ◽  
Gstm1 Gene ◽  
Expression Of Genes ◽  
Immune Inflammation

Objective. to establish the connection of radiation-induced changes in gene expression with the realized pathology of the broncho-pulmonary and cardiovascular systems in Chornobyl clean-up workers. Materials and methods. We examined 314 male Chornobyl clean-up workers (main group; age (58.94 ± 6.82) years (M ± SD); min 33, max 79 years; radiation dose (411.82 ± 625.41) mSv (M ± SD); min 1.74, max 3600 mSv) with various nosological forms of cardiovascular and broncho-pulmonary pathology (BPP) and 50 subjects of the control group: age (50.50 ± 5.73) years (M ± SD); min 41, max 67 years. The relative level of BCL2, CDKN2A, CLSTN2, GSTM1, IFNG, IL1B, MCF2L, SERPINB9, STAT3, TERF1, TERF2, TERT, TNF, TP53, CCND1, CSF2, VEGFA genes expression was determined in peripheral blood leukocytes by real-time PCR (7900 HT Fast Real-Time PCR System (Applied Biosystems, USA)). The «gene-disease» association was determined on statistical models stratified separately for each disease and gene. Logistic regression was used to calculate the odds ratio. Results. Increased GSTM1 gene expression and no changes in angiogenesis-related VEGFA gene expression were found in the main group of patients with coronary heart disease (CHD). It was established overexpression of TP53, VEGF and IFNG genes in the group of patients with arterial hypertension (AH). At combination of these diseases an increase of expression of СSF2, TERF1, TERF2 genes was established. The detected changes demonstrate an activation of the antioxidative defense system in patients with CHD, while AH is associated with the expression of genes of angiogenesis and immune inflammation. It was shown an increase in the expression of genes associated with apoptosis and kinase activity (BCL2, CLSTN2, CDKN2), immune inflammation (CSF2, IL1B, TNF) in Chornobyl clean-up workers with BPP. Expression of TP53 and GSTM1 (gene, associated with the glutathione system) was significantly upregulated in the group of individuals with chronic bronchitis, whereas in patients with chronic obstructive pulmonary disease, no increase was detected; the expression of SERPINB9 and MCF2L genes was downregulated. Conclusions. Changes in the expression of genes, associated with the development of somatic pathology in the remote period after irradiation, in particular the genes of the immune response and inflammatory reactions CSF2, IFNG, IL1B, TNF; expression of genes that regulate cell proliferation, aging and apoptosis TP53, BCL2, MCF2L, CDKN2A, SERPINB9, TERF1, TERF2, TERT; genes that regulate cell adhesion and angiogenesis CLSTN2, VEGF. Key words: gene expression, somatic pathology, radiation, Chornobyl.

The Alteration of SEPT5 Gene Expression in BCR-ABL Positive Cells and Its Possible Correlation with the Development and / or Progression of Chronic Myeloid Leukemia (CML)

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4415-4415
Author(s):  
Cintia Do Couto Mascarenhas ◽  
Anderson Ferreira Cunha ◽  
Ana Flavia Brugnerotto ◽  
Sheley Gambero ◽  
Joao Machado-Neto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Cell Lines ◽  
Real Time Pcr ◽  
Blood Donors ◽  
Mononuclear Cells ◽  
Conflicts Of Interest ◽  
Blood Platelets ◽  
Gene Expression Pattern ◽  
P Value

Abstract Abstract 4415 The CML is a clonal disease of stem cells and its main feature is the unregulated production of a tyrosine kinase protein called BCR-ABL, the progression of the disease to accelerated phase or blast crisis may be associated with genomic instability. Because of this, the use of tools for the study of gene expression could bring new insights in the understanding of these mechanisms in the CML. In a recent study using SSH libraries, we compared the gene expression pattern between granulocytes of health control and CML patients, and we identified the gene SEPT5 expressed only in CML patients. Although the studies in the literature, there is not a clear relationship between the expression of this gene and the development or progression of CML. SEPT5 is a member of nucleotide binding proteins called septins that were firstly described in yeast as cell division cycle regulatory proteins. This gene was reported in patients with AML translocated with MLL gene, in adult human brain and heart; it is also associated with alpha granules of human blood platelets. The aims of this study are to carry a functional analysis of SEPT5 in differents cells line and to study the relationship of this gene and the development and/or progression of CML. The gene expression evaluation was made in granulocytes, mononuclear cells and total leukocytes of CML patients and healthy blood donors in peripheral blood. It was also evaluated in bone marrow donors, in human cell lines (K562, HL60 and NB4) and in mice cell lines (BaF3/BCR-ABLp210 and BaF3T315I), performed by real-time PCR for the following genes: SEPT5, β-actin and GAPDH. Experiments were also performed to verify the difference between the chemotaxis of granulocytic cells from controls and patients by ELISA. Data were analysed statistically using the ANOVA followed by Dunnett’s test – P value of less than 0.05 was considered to be significant. The study was approved by the Research Ethic Committee of the Faculty of Medical Sciences of University of Campinas. The gene expression of SEPT5 was evaluated by real time PCR using the same samples used in the library construction to validate the results found in the SSH library. The data confirmed our previous results, showing that the SEPT5 expression is increased in all cells of patients compared to controls. The same results were observed when we studied the expression comparing individually patients and health blood donors, suggesting that this protein could be increased in all human cells that present the translocation BCR-ABL. The level of expression of this gene in HL60 and NB4 was significantly lower than in K562 cell line. The experiments with mice cell lines showed a higher expression of this gene in BaF3T315I when compared to BaF3BCR-ABLp210. We obtained a significant expression difference in all experiments (p <0.05). The spontaneous and stimulated with IL-8 chemotaxis assays used granulocytes and were assessed using chamber containing 96 wells. However, although the results suggest an increased chemotactic activity in patients, there were no significant differences (p<0.05) between controls and patients – regardless of whether the chemotaxis was spontaneous or stimulated with IL-8. In mammals the SEPT5 gene is associated with cellular processes such as exocytosis, apoptosis, leukemogenesis, carcinogenesis and neurodegeneration. Therefore, molecules capable of interacting with the septins, either at biochemical or molecular level, can bring information about their functions in cytokinesis. Studies indicate that the human septins can interact among themselves and with other components of the cytoskeleton – this may be a relevant observation regarding the function of this gene in cancer. The SEPT5 can be activated by different pathways – this may increase expression in translocated cells. Despite major advances in the treatment of CML, the treatments available are not capable of inactivating all the signaling pathways activated by BCR/ABL. Our results demonstrate that SEPT5 may be involved in the pathophysiology of CML. Also, it is clear the importance of the study of pathways that could culminate in its high expression or the triggering of other unknown pathways involved in the development of CML. The increased expression of this gene may be related to disease progression, and finally, the identification of several important genes may lead to a better understanding of CML and helping to identify new therapeutic targets. FAPESP/INCT. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Evaluation of GNMT Gene Expression in Prostate Cancer Tissues using Real-Time PCR

2021 ◽  
Author(s):  
Niloofar Dehghani ◽  
Masoud Salehipour ◽  
Babak Javanmard
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Real Time ◽  
Real Time Pcr ◽  
Prostatic Hyperplasia ◽  
Expression Level ◽  
P Value ◽  
Cancer Tissue ◽  
Tissue Samples

Introduction: Prostate cancer is the second most common cancer and the leading cause of cancer-related deaths worldwide. In the present study, the expression level of glycine N-methyl transferase gene (GNMT) was investigated in prostate cancer tissue. The GNMT enzyme is encoded by the GNMT gene. Increased GNMT gene expression increases the conversion of glycine to sarcosine and results in the elevated levels of sarcosine in blood and urine. Methods: The expression level of GNMT gene in tissue samples of patients with prostate cancer was compared with those with benign prostatic hyperplasia using Real-Time PCR technique. Results: The GNMT gene expression level increased significantly in prostate cancer patients compared with those with benign prostatic hyperplasia (p-value <0.001). In addition, the expression level of GNMT gene was stage-dependent and  significant increases were observed in all stages of prostate cancer compared with those with benign prostatic hyperplasia (p-value <0.001). Conclusion: The concentration of sarcosine is controlled by GNMT and it seems that increasing the expression level of GNMT gene increases the level of sarcosine concentration. Thus, it appears that increased levels of GNMT expression occur in the early stages of prostate cancer. Therefore, periodic measurement of GNMT expression levels can detect prostate cancer before it forms a cancer cell and invades other tissues.

65 SCRIPTAID CORRECTS GENE EXPRESSION OF A FEW ABERRANTLY REPROGRAMMED TRANSCRIPTS IN NUCLEAR TRANSFER PIG BLASTOCYST STAGE EMBRYOS

2011 ◽  
Vol 23 (1) ◽  
pp. 138
Author(s):  
K. M. Whitworth ◽  
J. Zhao ◽  
L. D. Spate ◽  
R. S. Prather
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Real Time ◽  
Histone Deacetylase ◽  
Real Time Pcr ◽  
Nuclear Transfer ◽  
Transcriptional Profiling ◽  
Donor Cell ◽  
Blastocyst Stage ◽  
Hdac Activity

Scriptaid is a histone deacetylase inhibitor (HDACi) that can increase cloning efficiency. The objective of this study was to identify aberrantly reprogrammed transcripts by performing transcriptional profiling between in vivo (IVV), nuclear transfer (NT) blastocyst stage embryos and the donor cell line (cells). This was followed by measuring HDAC activity (Epigentek) in zygotes and by real-time PCR on a selected subset of genes at the blastocyst stage to determine if Scriptaid treatment (NTS) corrected the aberrant gene expression. NTS embryos were treated with 500 nM Scriptaid for 14 h after activation. NT and NTS embryos were transferred into gilts on Day 0 or 1 of oestrus and collected 6 days later by uterine flush. IVV samples were collected on Day 8 of gestation. 3 pools of 10 to 15 embryos and cells were collected for each treatment and analysed twice. For transcriptional profiling, total RNA was isolated by using Trizol (Invitrogen, Carlsbad, CA, USA), amplified by using an Ovation Ribo-SPIA linear amplification kit (Nugen), labelled with Cy5 and compared to reference labelled with Cy3. Lowess normalization and analysis was performed in Genespring 7.3.1. ANOVA was performed with the Benjamini and Hochberg False Discovery Rate. Transcripts that were different between IVV and NT (P ≤ 0.20) and significantly different from the donor cell line (P ≤ 0.05) were classified as being aberrantly reprogrammed. This comparison resulted in 119 under- and 60 over-compensated transcripts. Functional annotation classification was performed in DAVID and identified under-compensated pathways (oxidative phosphorylation and protein biosynthesis) and over-compensated pathways (chromatin packaging/remodelling and protein complex assembly). Fourteen transcripts were chosen for real-time PCR validation and evaluation of the effect of Scriptaid. Relative gene expression was compared between IVV, NT, NTS, and cells by the comparative Ct method with SYBR Green Supermix (Bio-Rad) and statistical analysis was performed in SAS 9.1 (SAS Institute Inc., Cary, NC, USA) by using a least significant difference test (P ≤ 0.05). NTS embryos had 3 transcripts returning to the same level as IVV (H3F3A, CAPG, and SEPT7). The level of the majority of the transcripts (8/14) was not affected by NTS treatment, e.g. histone deacetylase SIRT1 and H1 histone, member 0 (H1F0). However, Scriptaid treatment caused COX5A to be further over compensated in NTS with expression levels higher than IVV and NT. 2 transcripts had expression levels that were lower in NTS compared to both IVV and NT including GPD1L and EIF3E. Scriptaid treatment significantly affected gene expression in 6 of the 14 transcripts evaluated. Scriptaid treatment of the reconstructed zygotes did not affect the majority of the transcripts when measured at the blastocyst stage. HDAC activity was significantly reduced in NTS compared to NT 1-cell stage embryos (P ≤ 0.038). While Scriptaid reduced HDAC activity, it returned only a few genes to normal IVV levels. This project was supported in part by the USDA NRI (2006-35203-17282) and Food for the 21st Century.

scholarly journals PENGARUH EKSTRAK PROPOLIS TERHADAP EKSPRESI PROTEIN Bcl2, p21, DAN INDUKSI APOPTOSIS PADA SEL HELA

Biomedika ◽  
2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Tri Hardi Susanto ◽  
Suradi Maryono ◽  
Bambang Purwanto
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Control Group ◽  
Hela Cell Line ◽  
Control Group Design ◽  
P21 Protein ◽  
Group Design ◽  
Post Test

Kanker serviks merupakan penyebab kematian ketiga akibat kanker pada wanita di dunia. Di Indonesia, kanker serviks merupakan penyebab kematian utama perempuan dalam tiga dasa warsa terakhir. Berbagai strategi terapi pengobatan kanker serviks dengan menggunakan terapi bedah, radioterapi, dan kemoterapi maupun kombinasi ketiganya relatif belum optimal. Setiap abnormalitas pada jalur apoptosis dapat sebagai target terapi kanker. Pendekatan yang menarik untuk dikembangkan adalah penggunaan kombinasi kemoterapi atau ko-kemoterapi. Propolis yang menunjukkan aktivitas proapoptosis pada berbagai jenis sel kanker, meliputi : kanker laring, kanker paru, kanker pankreas, kanker tiroid, kanker kolorektal, kanker payudara, kanker prostat dan glioma. Propolis merupakan salah satu produk natural yang potensial untuk dikembangkan sebagai agen ko-kemoterapi. Tujuan penelitian ini untuk mengetahui pengaruh pemberian propolis yang berasal dari Kerjo, Karanganyar, Indonesia terhadap induksi proses apoptosis dan aktivitas antiproliferasi, terutama terkait dengan penekanan ekspresi protein Bcl2 dan peningkatan aktivasi p21 pada kultur sel HeLa (cell line kanker servik). Penelitian ini merupakan penelitian eksperimental laboratorik dengan menggunakan post test with control group design. Penelitian dilakukan pada kultur sel HeLa (sel kanker servik) dengan pemberian propolis. Pengamatan ekspresi p21 dan ekspresi protein Bcl2 dilakukan dengan metode imunositokimia, sedangkan pengamatan induksi apoptosis dilakukan dengan flowcytometry. Hasil penelitian menunjukkan ekspresi rata-rata Bcl2 pada kelima kelompok yaitu kontrol 84,60 ± 1,34 µg/ml, EEP 1/2 IC50  62,33 ±4,58, EEP IC50  33,98 ± 2,11 µg/ml, EEP 2 IC50 22,16 ± 3,41 µg/ml, Cisplatin 13,09±4,38 µg/ml. Terdapat perbedaan bermakna ekspresi Bcl2  antara kelompok uji dibandingkan kelompok kontrol (p < 0,001). Rata-rata ekspresi p21 pada kelima kelompok yaitu pada grup kontrol 1,70 ± 0,67 µg/ml, EEP 1/2 IC50  65,92 ± 0,40, EEP IC50  82,76 ± 3,03 µg/ml, EEP 2 IC50  86,86 ± 3,33 µg/ ml, Cisplatin 93,19 ± 3,02 µg/ml. Terdapat perbedaan bermakna ekspresi p21  antara kelompok uji dibandingkan kelompok kontrol (p < 0,001). Penelitian ini menyimpulkan bahwa pemberian ekstrak etanol propolis mempunyai pengaruh terhadap penekanan ekspresi Bcl2, peningkatan ekspresi p21, dan induksi apoptosis pada kultur sel kanker servik (HeLa cell line).Kata Kunci: EEP, p21, protein Bcl2, HeLa cell line

scholarly journals Extracellular matrix (ECM) and cell adhesion molecules (CAMs) gene expression in brain GD-10, hippocampal cell (mHT-22 Cell Line), human glioblastoma-astrocytoma (hLN-405), and rat glioma cell (rF98) using real time PCR.

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Yulia Irnidayanti ◽  
Win Darmanto
Keyword(s):  
Gene Expression ◽  
Cell Adhesion ◽  
Cell Line ◽  
Real Time ◽  
Real Time Pcr ◽  
Glioma Cell ◽  
Gene Copy ◽  
Human Glioblastoma ◽  
Rat Glioma ◽  
Hippocampal Cell

The aim of this study is to compare ECM and CAMs gene expression in hippocampal cell, glioblastoma-astrocytoma and glioma cell using real time PCR at gestation day of 10 (GD-10) Result of real time PCR showed that expression level of extracelluler matrix of vimentin was higher than the expression of fibronectin, Neural cell Adhesion molecule (Ncam), neurofilament high (Nfh), neurofilament medium (NFm), neurofilament low (Nfl) and tenascin. The expression of vimentin in the brain also shows the highest when compare to the expression in the human glioblastoma-astrocytoma (hLN-405), rat glioma cell (rF98) and mouse hippocampal cell line (mHT-22). The number of vimentin gene copy was 538.554, whereas in the culture of hLN-405 was 2.246.309, in culture of rF98 was 974.368 and mHT-22 was 1.542.529. These findings suggested that vimentin most dominant expressed compare to the another gen. Expression of vimentin was gradually lower from astrocyte cell (hLN-405 or human glioblastoma-astrocytoma), then in hippocampus cell,  glioma cell and embryonic brain cell. ________________________________________GRAPHICAL ABSTRACT

scholarly journals Association of Setmar Expression with Clinical Characteristics in Chronic Lymphocytic Leukemia

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4815-4815
Author(s):  
Flavia Z Piazera ◽  
Doralina Amaral Rabello ◽  
Felipe Araújo Saldanha ◽  
Fábio Pitella Silva ◽  
Fábio Morato Oliveira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Characteristics ◽  
Tumor Stage ◽  
Healthy Controls ◽  
Set Domain ◽  
Relative Expression ◽  
Wbc Count ◽  
Lower Expression

Abstract INTRODUCTION: Epigenetic changes have proved increasingly important in the genesis of various tumors including acute and chronic lymphoid malignancies.Its basic features do not cause changes in the DNA sequence, but removing selective expression of genes dependent DNA packaging level. The histone methylation is one of the leading and most studied epigenetic events. SETD family of methyltransferases comprises 10 genes encoding proteins with SET domain. SETMAR encodes a protein that contains an N-terminal SET domain and a C-terminal mariner transposase domain. SETMAR-catalyzed methylation of H3K4 and H3K36 may lead to an open chromatin structure, which may facilitate its transposase-dependent processes. SETMAR was associated with carcinogenesis of acute myeloid leukemia by losing disjuntion checkpoint in cells treated with inhibitors of protein topo II. However, the role of SETMAR in CLL leukemogenesis remains unknown. METHODS: In the present study, we evaluated the relative expression of SETMAR between a group of 59 CLL patients and 10 healthy controls by real-time PCR. As normal controls, we used peripheral blood mononuclear cells (PBMC) from 10 age-matched hematological healthy donors (age 50 to 84 years).We analyzed the correlation between SETMAR expression patterns with CLL clinical characteristics such as chromosomal aberrations, ZAP-70 expression and white blood cell (WBC) count. Total RNA was isolated and cDNAs were synthesized. Quantification of SETMAR was performed by Real Time PCR (qPCR) and normalized to endogenous (beta-actin) expression. Results were analyzed by the comparative2-ΔΔCt method. The amount of target gene, normalized to the endogenous control gene and relative to a reference sample, was converted into relative quantification. Based on the continuous distribution of SETMAR expression on CLL samples, we adopted the median value as the cutoff to dichotomize CLL patients in "low" and "high" SETMAR expression. Clinical and laboratory information were then compared between groups. Statistical analysis was performed by GraphPad Prism version 5. The Mann-Whitney U-test was used to examine differences between SETMAR expression (low or high) groups versus platelets, WBC count and ZAP-70 status and also to compare the groups of CLL patients with normal versus abnormal karyotypes. Association of SETMAR expression profile with karyotype was done using Fisher's exact test. RESULTS: The clinical characteristics of CLL patient cohort used in this study is summarized in table 1. Initially, we compared SETMAR gene expression profiles between CLL patients and control samples, using the Mann-Whitney test. SETMAR gene expression was higher in CLL samples (p= 0.00117),with statistical difference (p<0.05) (Fig. 1). Table 1. Clinical and laboratorial characteristics of CLL patients. Characteristics PATIENTS (%) GENDER Male 24(40,7%) Female 35(59,3%) AGE 63 (32-98 years) TUMOR STAGE Binet A 39(66%) Binet B 13(22%) Binet C 7(12%) CYTOGENETIC ANALYSIS 13q deletion 5(8,3%) 17p deletion 4(6,7%) 12tryssomy 16(26,7%) Normal 15(25%) Others 20(33,3%) ZAP-70 expression >20% 39(67,2%) <20% 19(32,8%) CLL patients with lower expression of SETMAR had a higher WBC count and a higher incidence of cytogenetic abnormalities (CTG) (p=0.001) when compared to those with higher expression (p=0.0262) (Fig. 2). However, differential SETMAR expression had no impact on platelets count (p = 0.092), ZAP-70 protein expression (p = 0.25) and tumor stage of Binet (p= 0.38). Figure 2- A) SETMAR relative expression gene and cytogenetic abnormalities, with p=0,001 and B) SETMAR relative expression and WBC counts with p= 0,0262. The cohort of 59 clinical specimens of CLL patients used in the present study revealed quite heterogeneous patterns of SETMAR expression and its association with clinical variables. Future efforts will be necessary to increase the assessment of SETMAR expression in a larger number of CLL patients in order to evaluate its impact on survival, as well as to unveil its correlation with response to established cancer therapy and in the course of the disease. CONCLUSION: We observed that although the expression of SETMAR is elevated in the majority of CLL patients when compared to healthy controls, a lower expression of SETMAR in patients is associated with chromosomal instability and progression of the tumor mass (increased leukocytosis). Figure 2. Figure 2. Figure 3. Figure 3. Disclosures No relevant conflicts of interest to declare.

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