scholarly journals Phytochemical and biological investigation of Indian medicinal plant of givotia rottlerifromis griff

2021 ◽  
Vol 3 (2) ◽  
pp. 54-63
Author(s):  
Mohanraj V ◽  
Sakthivel R.V ◽  
Karthick M
Keyword(s):  
2D Nmr ◽  
Docking Studies ◽  
Hiv Protease ◽  
Melting Point Determination ◽  
Biological Investigation ◽  
Antimicrobial Studies ◽  
Soxhlet Apparatus ◽  
Point Determination ◽  
Pure Methanol ◽  
Antibacterial And Antifungal

A study on givotia rottlerifomis griff shows that studies on a tremendous of pharmacological and physiological activities and other applications. Moreover, givotia rottlerifomis griff has diverse physiological effects. This tempted us to use the bark of givotia rottlerifomis griff for extraction of new compound from the extract. Attempt is made to develop an efficient method for isolation of compounds from the barks of givotia rottlerifomis griff. Our scheme of work is to separate a portion by treatment with pure methanol (CH3OH) using soxhlet apparatus at boiling temperature and the sample is crystallized to separate to the pure compound. The compound was identified further by chromatographic studies, melting point determination, and spectral analysis (IR, 1D and 2D NMR) etc. Furthermore, antimicrobial studies are tested various antibacterial and antifungal species and also molecular docking studies are checked with HIV protease with Schrodinger 9.5 software.

scholarly journals Synthesis, Characterization and Antimicrobial Studies of Schiff Base Ligand-Derived from Amoxicillin and Benzaldehyde

2016 ◽  
Vol 19 (2) ◽  
pp. 211-214
Author(s):  
Asif Iqbal ◽  
Farhana Hoque
Keyword(s):  
Schiff Base ◽  
Pharmaceutical Journal ◽  
Spectroscopic Techniques ◽  
Disc Diffusion ◽  
Melting Point Determination ◽  
Antibacterial And Antifungal Activities ◽  
Antimicrobial Studies ◽  
Point Determination ◽  
Antibacterial And Antifungal

Advanced research into synthetic chemistry has pointed out several organic compounds with antimicrobial potential. Schiff bases are one of such compounds. This research aims at the synthesis of a Schiff base out of a fairly common antibiotic amoxicillin and benzaldehyde. The synthesized compound was characterized by different spectroscopic techniques. It was also subjected to melting point determination, TLC and solubility tests. The Schiff base was screened for antibacterial and antifungal activities in-vitro by disc diffusion method.Bangladesh Pharmaceutical Journal 19(2): 211-214, 2016

Substituted 6,7-dimethoxy-5-oxo-2,3,5,9b-tetrahydrothiazolo[2,3-a]isoindole- 3-1,1-dioxide Derivatives with Antimicrobial Activity and Docking Assisted Prediction of the Mechanism of their Antibacterial and Antifungal Properties

2020 ◽  
Vol 20 (29) ◽  
pp. 2681-2691
Author(s):  
Athina Geronikaki ◽  
Victor Kartsev ◽  
Phaedra Eleftheriou ◽  
Anthi Petrou ◽  
Jasmina Glamočlija ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Drug Targets ◽  
Bacterial Species ◽  
Pharmaceutical Research ◽  
Docking Studies ◽  
Fungal Species ◽  
Docking Analysis ◽  
E Coli ◽  
Antibacterial And Antifungal

Background: Although a great number of the targets of antimicrobial therapy have been achieved, it remains among the first fields of pharmaceutical research, mainly because of the development of resistant strains. Docking analysis may be an important tool in the research for the development of more effective agents against specific drug targets or multi-target agents 1-3. Methods: In the present study, based on docking analysis, ten tetrahydrothiazolo[2,3-a]isoindole derivatives were chosen for the evaluation of the antimicrobial activity. Results: All compounds showed antibacterial activity against eight Gram-positive and Gram-negative bacterial species being, in some cases, more potent than ampicillin and streptomycin against all species. The most sensitive bacteria appeared to be S. aureus and En. Cloacae, while M. flavus, E. coli and P. aeruginosa were the most resistant ones. The compounds were also tested for their antifungal activity against eight fungal species. All compounds exhibited good antifungal activity better than reference drugs bifonazole (1.4 – 41 folds) and ketoconazole (1.1 – 406 folds) against all fungal species. In order to elucidate the mechanism of action, docking studies on different antimicrobial targets were performed. Conclusion: According to docking analysis, the antifungal activity can be explained by the inhibition of the CYP51 enzyme for most compounds with a better correlation of the results obtained for the P.v.c. strain (linear regression between estimated binding Energy and log(1/MIC) with R 2 =0.867 and p=0.000091 or R 2 = 0.924, p= 0.000036, when compound 3 is excluded.

scholarly journals Synthesis of 3-Ferrocenyl Isocoumarins

2020 ◽  
Vol 36 (05) ◽  
pp. 976-979
Author(s):  
Neeta Sinha
Keyword(s):  
Spectral Analysis ◽  
Melting Point ◽  
Phosphoric Acid ◽  
Polyphosphoric Acid ◽  
Aluminium Chloride ◽  
Melting Point Determination ◽  
Anhydrous Aluminium Chloride ◽  
Point Determination ◽  
Derivatives Of

The derivatives of 3-ferrocenyl isocoumarin were synthesized by the condensation of substituted homothphalic anhydride with ferrocene using phosphoric acid or anhydrous aluminium chloride as cyclising agent. Substituted homophthalic acid did not condense with ferrocene so homophthalic acids were converted into their anhydride and then allowed to react with ferrocene in the presence of polyphosphoric acid or in the presence of anhydrous aluminium chloride using dichloromethane as the solvent to give 3-ferrocenyl isocoumarins. 7-Methoxy, 6-methyl, 5,7-dihydroxy, 6,7-dimethoxy and 5,7-dimethoxy derivatives of 3-ferrocenyl isocoumarin were synthesized. All the compounds were characterised by melting point determination, elemental and spectral analysis.

scholarly journals ANTIMICROBIAL STUDIES OF DIFFERENT EXTRACTS OF HOLOPTELEA INTEGRIFOLIA (ROXB.) LEAVES

2020 ◽  
pp. 191-193
Author(s):  
REKHA TRIPATHI
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Disk Diffusion Method ◽  
Rhizoctonia Bataticola ◽  
Antimicrobial Studies ◽  
Almost All ◽  
Antibacterial And Antifungal ◽  
Antifungal Efficacy

Objective: The aim of this study was to examine various extracts of leaves of Holoptelea integrifolia against some test bacteria and test fungi. Methods: Disk diffusion method was adopted for the assessment of antimicrobial activity. Amikacin and nystatin were used as standard drugs for antibacterial and antifungal activity, respectively. Results: The screening data indicated that all four extracts showed antibacterial activity against Staphylococcus aureus, but the growth of this bacteria was inhibited the most by the aqueous extract. In the case of antifungal efficacy, all the extracts inhibited the growth of almost all the test fungi. Petroleum ether and benzene extracts showed maximum efficacy against Aspergillus flavus, whereas methanolic extract and aqueous extract inhibited the growth of Rhizoctonia bataticola significantly. Conclusion: Different extracts of leaves of H. integrifolia were significantly active against selected test fungi and they can be a harmless alternative of expensive conventional medicines.

scholarly journals Synthesis, Characterization, in vitro Antimicrobial Evaluation and in silico Molecular Docking and ADME Prediction of 4-Chlorophenyl Furfuran Derivatives bearing Pyrazole Moieties

2020 ◽  
Vol 32 (6) ◽  
pp. 1482-1490
Author(s):  
Manju Mathew ◽  
Raja Chinnamanayakar ◽  
Ezhilarasi Muthuvel Ramanathan
Keyword(s):  
Molecular Docking ◽  
In Silico ◽  
Docking Study ◽  
Docking Studies ◽  
Moderate Activity ◽  
Molecular Docking Study ◽  
Adme Prediction ◽  
Antimicrobial Studies ◽  
In Silico Molecular Docking

A series of 1-(5-(5-(4-chlorophenyl)furan-2-yl)-4,5-dihyropyrazol-1-yl ethanone (5a-h) was synthesized through E-(3-(5-(4-chloro-phenyl)furan-2-yl)-1-phenylprop-2-en-1-one (3a-h) with hydrazine monohydrate and sodium acetate. Totally, eight compounds were synthesized and their structures were elucidated by infrared, 1H & 13C NMR, elemental analysis, antimicrobial studies, in silico molecular docking studies and also in silico ADME prediction. Antimicrobial studies of the synthesized compounds showed good to moderate activity against the all the stains compared with standard drugs. in silico Molecular docking study was carried out using bacterial protein and BC protein. Synthesized compounds (5a-h) showed good docking score compared with ciprofloxacin. Antimicrobial study was carried out for 4-chlorophenyl furfuran pyrazole derivatives (5a-h). The results of assessment of toxicities, drug likeness and drug score profiles of compounds (5a-j) are promising

Synthesis, SAR and molecular docking studies of benzo[d]thiazole-hydrazones as potential antibacterial and antifungal agents

2017 ◽  
Vol 27 (14) ◽  
pp. 3148-3155 ◽  
Author(s):  
Gao-Feng Zha ◽  
Jing Leng ◽  
N. Darshini ◽  
T. Shubhavathi ◽  
H.K. Vivek ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Antifungal Agents ◽  
Docking Studies ◽  
Molecular Docking Studies ◽  
Antibacterial And Antifungal

Synthesis, biological investigation, calf thymus DNA binding and docking studies of the sulfonyl hydrazides and their derivatives

2016 ◽  
Vol 1107 ◽  
pp. 99-108 ◽  
Author(s):  
Shahzad Murtaza ◽  
Saima Shamim ◽  
Naghmana Kousar ◽  
Muhammad Nawaz Tahir ◽  
Muhammad Sirajuddin ◽  
...  
Keyword(s):  
Dna Binding ◽  
Docking Studies ◽  
Calf Thymus Dna ◽  
Biological Investigation ◽  
Calf Thymus

scholarly journals Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa

2015 ◽  
Vol 10 (10) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Manne Sumalatha ◽  
Rachakunta Munikishore ◽  
Aluru Rammohan ◽  
Duvvuru Gunasekar ◽  
Kotha Anil Kumar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Methanol Extract ◽  
2D Nmr ◽  
Docking Studies ◽  
Selective Inhibitor ◽  
Pueraria Tuberosa ◽  
Nmr Techniques ◽  
Cox 2 ◽  
Plant Sources ◽  
Cox 1

Bioassay-guided fraction of the methanol extract of the roots of Pueraria tuberose DC yielded puerarin, an isoflavone C-glycoside (PT-1), isoorientin, a flavone C-glycoside (PT-2) and mangiferin, a xanthone C-glycoside (PT-3). The extracts and the isolated compounds were screened for potent antiinflammatory components inhibiting the cyclooxygenases (COX-1 and COX-2) and 5-lipoxygenase (5-LOX), the target enzymes of inflammation, by employing spectroscopic/polorographic methods. Among these, isoorientin was found to be a potent inhibitorof COX-2with an IC50 value of 39 μM. Docking studies were carried out to understand the interactions of isorientin (PT-2) with COX-2. The structures of the isolates were determined by mass spectrometry and 2D-NMR techniques including HSQC, HMBC, NOESY and 1H-1H COSY experiments. Although isoorientin and mangiferin have been reported from several plant sources, this is the first report of their isolation from a Pueraria species.

scholarly journals Antimicrobial Activity of Nitrogen-Containing 5-α-Androstane Derivatives: In Silico and Experimental Studies

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 224 ◽  
Author(s):  
Lela Amiranashvili ◽  
Nanuli Nadaraia ◽  
Maia Merlani ◽  
Charalampos Kamoutsis ◽  
Anthi Petrou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
In Silico ◽  
Web Applications ◽  
Experimental Studies ◽  
Docking Studies ◽  
Minimum Fungicidal Concentration ◽  
Antibacterial And Antifungal ◽  
Mic Minimum Inhibitory Concentration ◽  
Androstane Derivatives

We evaluated the antimicrobial activity of thirty-one nitrogen-containing 5-α-androstane derivatives in silico using computer program PASS (Prediction of Activity Spectra for Substances) and freely available PASS-based web applications (such as Way2Drug). Antibacterial activity was predicted for 27 out of 31 molecules; antifungal activity was predicted for 25 out of 31 compounds. The results of experiments, which we conducted to study the antimicrobial activity, are in agreement with the predictions. All compounds were found to be active with MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values in the range of 0.0005–0.6 mg/mL. The activity of all studied 5-α-androstane derivatives exceeded or was equal to those of Streptomycin and, except for the 3β-hydroxy-17α-aza-d-homo-5α-androstane-17-one, all molecules were more active than Ampicillin. Activity against the resistant strains of E. coli, P. aeruginosa, and methicillin-resistant Staphylococcus aureus was also shown in experiments. Antifungal activity was determined with MIC and MFC (Minimum Fungicidal Concentration) values varying from 0.007 to 0.6 mg/mL. Most of the compounds were found to be more potent than the reference drugs Bifonazole and Ketoconazole. According to the results of docking studies, the putative targets for antibacterial and antifungal activity are UDP-N-acetylenolpyruvoylglucosamine reductase and 14-α-demethylase, respectively. In silico assessments of the acute rodent toxicity and cytotoxicity obtained using GUSAR (General Unrestricted Structure-Activity Relationships) and CLC-Pred (Cell Line Cytotoxicity Predictor) web-services were low for the majority of compounds under study, which contributes to the chances for those compounds to advance in the development.

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