scholarly journals Royal Jelly and Its SDS-PAGE Electrophoresis Profiles

2019 ◽  
pp. 17-20
Author(s):  
Hilal Ebru Çakır ◽  
Yakup Şirin ◽  
Sevgi Kolaylı
Keyword(s):  
Royal Jelly ◽  
Sds Page ◽  
Page Electrophoresis

scholarly journals Establishment of peritoneal liquid electrophoretogram from healthy horses and horses submitted to experimentally induced intestinal obstruction

2014 ◽  
Vol 66 (3) ◽  
pp. 665-671 ◽  
Author(s):  
A.F.S. Nogueira ◽  
P.A. Di Filippo ◽  
L.A. Anai ◽  
M.C. Vieira ◽  
K.M.M.G. Simplício ◽  
...  
Keyword(s):  
Intestinal Obstruction ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Immunoglobulin A ◽  
Control Group ◽  
Protein Levels ◽  
Sds Page ◽  
Significant Difference ◽  
Page Electrophoresis ◽  
Experimentally Induced

The initial inflammatory stages of the colic syndrome include changes known as acute phase response. The aim of this study was to contribute with the establishment of reference values concerning the electrophoretogram of peritoneal liquid from healthy horses and horses submitted to experimentally induced intestinal obstruction. Twenty-one horses were allotted in four groups: duodenal obstruction (DG), ileum obstruction (IG), left-dorsal colon obstruction (MG), and control group (CG). Peritoneal liquid was sampled before obtruction (T0), with 3 hours of obstruction (T3) and 6, 30, 102 and 174 hours after desobstructing (T6, T30, T102 and T174, respectively). Total protein levels were determined by the biuret method and protein fractions were obtained by SDS-PAGE electrophoresis. The acute phase proteins (APP) identified were Immunoglobulin-A, ceruloplasmin, transferrin, albumin, α1-antitrypsin, heavy and light chains of immunoglobulin-G, haptoglobin, α1-acid glycoprotein and a still unnamed protein, which was called P24. There was no difference (P>0.3) in protein levels among groups, although a significant difference (P>0.05) was observed between distinct experimental moments in each group evidencing a higher response of the APP in the obstructed groups. The APP fractioning of the peritoneal liquid was standardized to establish a standard curve for healthy equines and those submitted to induced intestinal obstruction. Moreover, it was verified that the SDS-PAGE electrophoresis was sensitive and effective to help diagnose abdominal inflammatory processes.

scholarly journals Growth characteristics and dynamics of protein synthesis in callus cultures from Glycine wightii (Wight & Arn.) Verdc.

2005 ◽  
Vol 29 (6) ◽  
pp. 1161-1166 ◽  
Author(s):  
André Luis Coelho da Silva ◽  
Cecília Sulzbacher Caruso ◽  
Renato de Azevedo Moreira ◽  
Ana Cecília Góes Horta
Keyword(s):  
Protein Synthesis ◽  
Growth Curve ◽  
Dry Weight ◽  
Callus Cultures ◽  
Exponential Phase ◽  
Cotyledon Explants ◽  
Ph Values ◽  
Freeze Dried ◽  
Sds Page ◽  
Page Electrophoresis

Cotyledon explants were first cultured on MS medium supplemented with 4.52 M 2,4-D and 0.46 mM kinetin. The development of the calli was followed (0, 4, 8, 12, 16, 20, 24, 28 and 32 days after) and the growth curve was determined, based in fresh and dry weight. The growth curve presented sigmoidal form with four distinct phases. The highest growth percentage was observed at the exponential phase and the lowest at the stationary phase. These results indicated that cotyledon callus subculture should be performed 20 days after inoculation. The calli obtained after a period of 28 days were freeze dried, macerated and submitted to extraction with buffers of different pH values (2.6; 4.0; 6.0; 8.0 and 10.0) and the proteins in the extracts were determined by Bradford method. The pH 8.0 buffer was the most efficient to extract the largest amount of protein. The amino acid analyses calli showed a high content of aspartic acid and low content of metionin. The dynamics of protein synthesis in calli was followed by SDS-PAGE electrophoresis.

scholarly journals Characterization and purification of a novel dATP-binding protein in eukaryotes

1992 ◽  
Vol 287 (3) ◽  
pp. 871-879 ◽  
Author(s):  
A Dalton ◽  
D P Hornby ◽  
S P Langston ◽  
G M Blackburn
Keyword(s):  
Binding Protein ◽  
Active Form ◽  
Direct Interaction ◽  
Nuclear Fraction ◽  
Yeast Saccharomyces Cerevisiae ◽  
Naturally Occurring ◽  
Sds Page ◽  
Marked Preference ◽  
High Degree ◽  
Page Electrophoresis

We characterized and purified an acidic dATP-binding protein, which, in its active form, resides in the nuclear fraction of a range of cells from mammals (including pig liver) and baker's yeast (Saccharomyces cerevisiae). This protein exhibits a high degree of specificity for the deoxy form of the naturally occurring nucleoside triphosphates and shows a marked preference for the purine deoxynucleoside triphosphates dATP and dGTP. The protein cleaves the terminal phosphate of dATP and appears to retain the dADP moiety of the nucleotide in a reaction that is resistant to both SDS and 8 M-urea. Fractionation of the nuclear preparation followed by non-denaturing PAGE and SDS/PAGE electrophoresis was sufficient to produce pure protein. The occurrence of this activity in all nuclei tested suggests that it plays an important role in nuclear metabolism. The specificity of the enzyme for deoxynucleoside triphosphates further suggests a role for this enzyme in DNA replication or repair, but the acidity of the protein argues against a direct interaction with DNA, and, indeed, the catalytic activity is not modulated by the inclusion of DNA in a variety of physical forms.

scholarly journals Quality, Microstructure, and Technological Properties ofSheepMeat Marinated in Three Different Ways

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Dessislava Borislavova Vlahova-Vangelova ◽  
Stefan Georgiev Dragoev ◽  
Dessislav Kostadinov Balev ◽  
Bahytkul Kajkenovna Assenova ◽  
Kumarbek Junusbekovich Amirhanov
Keyword(s):  
Protein Solubility ◽  
Amino Nitrogen ◽  
Sodium Lactate ◽  
Technological Properties ◽  
Lipid Fractions ◽  
Sds Page ◽  
Lipid And Protein Oxidation ◽  
Oil Emulsions ◽  
Prooxidant Action ◽  
Page Electrophoresis

The objective of this study was to explore the effect of 24 and 48 h alkaline (2% pentasodium tripolyphosphate), acid (2% sodium lactate), and water-oil marinating (water : sun flavor oil = 1 : 1 and 2% salt) as well as brine soaking (2% salt) on microstructure, changes in protein, and lipid fractions and technological properties of sheep (m. Longissimus dorsi). Strong myofibrillar fragmentation after 48 h alkaline marinating was observed. Significant swelling and increasing of spaces between myofibrils were found after 24 h brine soaking. Marinating in water-oil emulsions did not show a significant impact on the muscle microstructure. Alkaline and acid marinating as well as salt soaking promote the myofibrillar protein solubility and increased the free amino nitrogen content. After 24 h acid and 48 h alkaline marinating SDS-PAGE electrophoresis showed increasing of 25–30 kDa protein bands. The results obtained for the lipid and protein oxidation confirm prooxidant action of the sodium lactate (2%) and antioxidant effect of polyphosphates (2%) in marinated sheep.

scholarly journals Effect of Selenate on Viability and Selenomethionine Accumulation ofChlorella sorokinianaGrown in Batch Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Živan Gojkovic ◽  
Carlos Vílchez ◽  
Rafael Torronteras ◽  
Javier Vigara ◽  
Veronica Gómez-Jacinto ◽  
...  
Keyword(s):  
Large Subunit ◽  
Cell Activity ◽  
Protein Band ◽  
Chlorella Sorokiniana ◽  
Chloroplast Gene ◽  
Batch Cultures ◽  
Ultrastructural Studies ◽  
Sds Page ◽  
Sublethal Concentrations ◽  
Page Electrophoresis

The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green algaChlorella sorokinianagrown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L−1(212 μM) decreased growth rates for about 25% compared to control. A selenate EC50value of 45 mg·L−1(238.2 μM) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit ofChlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg−1of SeMet in 120 h of cultivation. This paper shows thatChlorella sorokinianabiomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.

scholarly journals Deoxygenation Affects Composition of Membrane-Bound Proteins in Human Erythrocytes

2016 ◽  
Vol 39 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Oksana G. Luneva ◽  
Svetlana V. Sidorenko ◽  
Olga O. Ponomarchuk ◽  
Artem M. Tverskoy ◽  
Aleksander A. Cherkashin ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Atp Release ◽  
Human Erythrocytes ◽  
Atp Content ◽  
Hypoxic Conditions ◽  
Sds Page ◽  
Membrane Bound ◽  
Molecular Origin ◽  
Free Environment ◽  
Page Electrophoresis

Background/Aims: ATP release from erythrocyte plays a key role in hypoxia-induced elevation of blood flow in systematic circulation. We have previously shown that hemolysis contributes to erythrocyte ATP release triggered by several stimuli, including hypoxia, but the molecular mechanisms of hypoxia-increased membrane fragility remain unknown. Methods: In this study, we compared the action of hypoxia on hemolysis, ATP release and the composition of membrane-bound proteins in human erythrocytes. Results: Twenty minutes incubation of human erythrocytes in the oxygen-free environment increased the content of extracellular hemoglobin by ∼1.5 fold. Paired measurements of hemoglobin and ATP content in the same samples, showed a positive correlation between hemolysis and ATP release. Comparative analysis of SDS-PAGE electrophoresis of erythrocyte ghosts obtained under control and deoxygenated conditions revealed a ∼2-fold elevation of the content of membrane-bound protein with Mr of ∼60 kDa. Conclusion: Deoxygenation of human erythrocytes affects composition of membrane-bound proteins. Additional experiments should be performed to identify the molecular origin of 60 kDa protein and its role in the attenuation of erythrocyte integrity and ATP release in hypoxic conditions.

scholarly journals Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development

2011 ◽  
Vol 41 (12) ◽  
pp. 2224-2229 ◽  
Author(s):  
Camila Argenta Fante ◽  
Patrícia de Fátima Pereira Goulart ◽  
José Donizeti Alves ◽  
Paôla de Castro Henrique ◽  
Daniela Deitos Fries
Keyword(s):  
Protein Content ◽  
Chronic Diseases ◽  
Total Protein ◽  
Electrophoretic Analysis ◽  
Stages Of Development ◽  
Major Barrier ◽  
Human Food ◽  
Soil Flooding ◽  
Sds Page ◽  
Page Electrophoresis

The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.

scholarly journals Characterization of Gelatin Profile of Chicken Broiler (Gallus domestica) Bone Using SDS-PAGE Electrophoresis

ALCHEMY ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 7 ◽  
Author(s):  
Dewi Yuliani ◽  
Dhienda Risa Awalsasi ◽  
Akyunul Jannah
Keyword(s):  
Ammonium Sulfate ◽  
Protein Concentration ◽  
Peptide Fragments ◽  
Strong Base ◽  
Chicken Bone ◽  
Sds Page ◽  
Hydrolysis Of ◽  
Β Chain ◽  
Page Electrophoresis

<p>Gelatin, a proteinaceous additive, is obtained from hydrolysis of collagen in the bone, hide and skin of animals. As natural product, gelatin has been applied in many industries with various functions. This study attempt to characterize gelatin profile of broiler chicken (<em>Gallus domestica</em>) using SDS-PAGE electrophoresis. The chicken bone was pretreated using a strong base, sodium hydroxide, producing type B gelatin. The gelatin was purified through precipitation using the variation of ammonium sulfate concentrations (40-70%) and dialysis using cellophane membrane. The purified gelatin was characterized through SDS-PAGE electrophoresis. Based on electrophoresis visualization, reduction of band intensity by ammonium sulfate 40% showed removal of small peptide fragments. The remained gelatin showed two major bands, α-chains and a β-chain with the respective molecular weight of ~135 and ~245 kDa. The protein content of the unpurified gelatin (E1) was 71.65±0.60 mg/L.  The purified E1 gelatins by 40-70% of ammonium sulfate addition contained 61.42±3.90, 60.45±1.36, 59.89±0.24, and 55.32±1.05 mg/L of protein concentration, respectively.</p><p> </p><p>Keywords: chicken bone, gelatin profile, protein electrophoresis</p>

Sign in / Sign up

Export Citation Format

Share Document