Marker-assisted identification of potential fertility restorers for the development of three-line hybrids in rice (Oryza sativa L.)

2020 ◽  
Vol 57 (3) ◽  
pp. 181-189
Author(s):  
Asma Majid ◽  
GA Parray ◽  
NR Sofi ◽  
Gazala H Khan ◽  
Showkat A Waza ◽  
...  
Keyword(s):  
Oryza Sativa L ◽  
Chromosome 1 ◽  
Limiting Factor ◽  
Kashmir Valley ◽  
Fertility Restorer ◽  
Yield And Quality ◽  
Restorer Genes ◽  
Increase Productivity ◽  
Fertility Restorers ◽  
Selection Of

Rice being a staple food crop of Kashmir valley, the focus is on enhancement of yield in order to meet the needs of ever-growing population.Identification of new parental lines is crucial for developing ecology-specific hybrids with ideal agronomic performance. Exploitation of heterosis in the form of hybrid rice technology can be one of the approaches to increase productivity in this crop, especially exploiting diversity among japonica lines can serve as an excellent route.A number of CMS lines suitable formountainous areas of Kashmir have been developed, however, the availability of promising restorer lines remains to be the major limitation for utilization of these lines.Identification of potential restorers acts as the main limiting factor for hybrid development in the Kashmir valley. Marker based screening for Rf3 and Rf4 fertility restorer genes can be helpful in rapid selection of restorer lines while dealing with the large quantity of genetic materials. In the present study, 100 rice germplasm were screened with the help of SSR markers, RM3148 and RM6100linked to Rf3 and Rf4 genes on chromosome 1 and 10, respectively. In total, 19 lines revealed the presence of both Rf3 and Rf4 genes. These lines amplified fertility restorer specific alleles for both the genes and may serve as potential restorers for obtaining heterotic rice hybrids. Further the germplasm lines were also evaluated for yield and quality traits.The present results would help in selection of suitable restorers along with preferred grain shape/size.

scholarly journals Identification of Suitable Restorers for WA-CMS Lines of Rice (Oryza sativa L.) through Conventional and Molecular Breeding Methods

2020 ◽  
Author(s):  
Anirban Nath ◽  
Disharee Nath ◽  
Chand Kumar Santra ◽  
Tapash Dasgupta
Keyword(s):  
Genetic Background ◽  
Molecular Breeding ◽  
Oryza Sativa L ◽  
Fertility Restoration ◽  
Male Sterile ◽  
Maintainer Line ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Restoration Potential ◽  
Fertility Restorer Genes

The current study extensively evaluates 51 genotypes for their fertility restoration potential using test crosses with five WA (wild abortive) Cytoplasmic Male Sterile lines namely IR58025A, IR6897A, IR79156A, IR80559A and APMS6A. Also the genotypes were screened using SSR markers RM6100 and RM10313, tightly linked with the fertility restorer genes Rf4 and Rf3 respectively. The two way approach helped in identifying potential restorers for five WA-CMS lines and also detected the presence of dominant Rf genes in their genetic background. The R-lines identified can be safely presumed to be strong restorers for consecutive A-lines they were crossed with. The study also identified a potential maintainer CN1039-9 for the A-line IR58025A. The maintainer line identified can be later exploited for developing new CMS lines.

scholarly journals Tagging of four Rf genes with selective genotyping analysis in rice (Oryza sativa L.)

Genetika ◽  
2017 ◽  
Vol 49 (3) ◽  
pp. 809-818
Author(s):  
Saeid Yarahmadi ◽  
Mohammad Sohani ◽  
Ali Ebadi ◽  
Masumeh Kheirgoo
Keyword(s):  
Genetic Distance ◽  
Hybrid Rice ◽  
Oryza Sativa L ◽  
Random Mating ◽  
Chromosome 1 ◽  
Chromosome 12 ◽  
Rice Seed ◽  
Rf Gene ◽  
F2 Population ◽  
Selection Of

Wild abortive type of cytoplasmic male sterility (WA-CMS) is commercially used for hybrid rice seed production. The linked markers can be used for selection of plants with desirable traits. Tagging of Rf genes was carried out using recessive and dominant class analysis in a large F2 population from the cross IR58025A?IR42686R. Pollen fertility and seed setting were evaluated at the flowering and maturity stages, respectively. Forty-seven highly sterile and 23 fertile homozygous plants were selected from F2 population for molecular marker assay. Four Rf genes identified in a good restorer line with high-quality derived from a random mating composite population at the International Rice Research Institute (IRRI). The genetic distance from Rf3 locus with flanking markers RM443 and RM315 on chromosome 1 was 3.7 and 21.2 cM, respectively. RM258, RM591, RM271 and RM6737 on the long arm of chromosome 10 were linked with the Rf6 gene with distance of 7.4, 22.6, 6 and 2.9 cM, respectively. Rf6 was flanked by RM6737 and RM591. The Rf4 gene located on chromosome 7 was linked with RM6344 at a genetic distance of 10.6 cM. RM519 and RM7003 were linked with other Rf gene on chromosome 12 at a genetic distance of 8.5 and 20.8 cM, respectively. Closely linked markers identified in this study could be used for marker assisted selection in a hybrid rice breeding program. A new Rf locus on chromosome 12 that designated Rf7 was linked with RM7003 and RM519.

scholarly journals Development of fertility restorers of winter oilseed rape with low glucosinolate content for the CMS Ogu-INRA system

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 123-127 ◽  
Author(s):  
R. Koprna ◽  
V. Kučera ◽  
I. Macháčková ◽  
J. Horáček ◽  
J. Ehrenbergerová
Keyword(s):  
Oilseed Rape ◽  
Scar Marker ◽  
Restorer Gene ◽  
Glucosinolate Content ◽  
Restorer Line ◽  
Winter Oilseed Rape ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Fertility Restorers ◽  
Selection Of

We have bred low glucosinolate (GSL) winter oilseed rape lines carrying the fertility restorer for the CMS Ogu-INRA system. The original restorer line BO20 contained 31μmol/g GSL in seeds, but by crossing this line with various low GSL CMS lines, followed by repeated selection of fertile segregants, we were able to obtain fertile lines with a mean GSL content in seeds of 11.8 μmol/g. This result confirmed that the gene(s) controlling the GSL content are not closely linked to the fertility restorer gene. The results confirm, that the SCAR marker SG34 is closely associated with the fertility restoring allele, and facilitates so the selection of fertile segregants; however, the marker is unable to distinguish between the homozygous RfRf and the heterozygous Rfrf genotypes.

Role of Farmers Participatory Vegetable Breeding in Kashmir

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
LAL SINGH ◽  
PRADEEP KUMAR SINGH ◽  
HARI BAKSH ◽  
SARVESH SINGH
Keyword(s):  
Resource Conservation ◽  
Vegetable Crops ◽  
Zero Tillage ◽  
Kashmir Valley ◽  
Full Control ◽  
Conservation Technology ◽  
Raised Bed ◽  
Selection Of ◽  
Conservation Technologies

Vegetable crops are conducting under Farmers Participatory Research Trial in Temperate regions of Kashmir Valley. The trials are designed and managed by farmers, the researchers have only advice for selection of the resource conservation technology (treatments). Farmers have full control over the selection of treatments to be used on his/her field. The main objectives of this type of research is to be established and demonstrate the benefits of resource conservation technologies like raised bed, furrow irrigated planting system, zero tillage etc. over the conventional practices. In these type of trial farmers are briefed about new practices. The participating farmers are encouraged to experiment their own and are given the full control over the selection of subset of resource conservation technologies to be tested on their fields with a view to assess farmer innovation and acceptability.

scholarly journals Identification of the Optimum Environments for the High Yield and Quality Traits of Lentil Genotypes Evaluated in Multi-Location Trials

2021 ◽  
Vol 13 (15) ◽  
pp. 8247
Author(s):  
Dimitrios N. Vlachostergios ◽  
Christos Noulas ◽  
Anastasia Kargiotidou ◽  
Dimitrios Baxevanos ◽  
Evangelia Tigka ◽  
...  
Keyword(s):  
Crude Protein ◽  
Agronomic Traits ◽  
High Yield ◽  
Breeding Programs ◽  
Growing Seasons ◽  
Yield And Quality ◽  
Food And Feed ◽  
Superior Genotypes ◽  
Selection Of ◽  
The Ideal

Lentil is a versatile and profitable pulse crop with high nutritional food and feed values. The objectives of the study were to determine suitable locations for high yield and quality in terms of production and/or breeding, and to identify promising genotypes. For this reason, five lentil genotypes were evaluated in a multi-location network consisting of ten diverse sites for two consecutive growing seasons, for seed yield (SY), other agronomic traits, crude protein (CP), cooking time (CT) and crude protein yield (CPY). A significant diversification and specialization of the locations was identified with regards to SY, CP, CT and CPY. Different locations showed optimal values for each trait. Locations E4 and E3, followed by E10, were “ideal” for SY; locations E1, E3 and E7 were ideal for high CP; and the “ideal” locations for CT were E3 and E5, followed by E2. Therefore, the scope of the cultivation determined the optimum locations for lentil cultivation. The GGE-biplot analysis revealed different discriminating abilities and representativeness among the locations for the identification of the most productive and stable genotypes. Location E3 (Orestiada, Region of Thrace) was recognized as being optimal for lentil breeding, as it was the “ideal” or close to “ideal” for the selection of superior genotypes for SY, CP, CT and CPY. Adaptable genotypes (cv. Dimitra, Samos) showed a high SY along with excellent values for CP, CT and CPY, and are suggested either for cultivation in many regions or to be exploited in breeding programs.

scholarly journals Mutator-Induced Mutations of the rf1 Nuclear Fertility Restorer of T-Cytoplasm Maize Alter the Accumulation of T-urfl3 Mitochondrial Transcripts

Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1383-1394
Author(s):  
Roger P Wise ◽  
Carren L Dill ◽  
Patrick S Schnable
Keyword(s):  
Pollen Fertility ◽  
Fertility Restoration ◽  
Mitochondrial Rna ◽  
Induced Mutations ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Mitochondrial Transcripts ◽  
Ecori Restriction ◽  
Fertility Restorer Genes

Abstract Dominant alleles of the rf1 and rf2 nuclear-encoded fertility restorer genes are necessary for restoration of pollen fertility in T-cytoplasm maize. To further characterize fertility restoration mediated by the Rf1 allele, 123,500 gametes derived from plants carrying the Mutator transposable element family were screened for rf1-mutant alleles (rf1-m) Four heritable rf1-m alleles were recovered from these populations. Three rf1-m alleles were derived from the progenitor allele Rf1-IAl53 and one was derived from Rf1-Ky21. Cosegregation analysis revealed 5.5- and 2.4kb Mu1-hybridizing EcoRI restriction fragments in all of the male-sterile and none of the male-fertile plants in families segregating for rf1-m3207 and rf1-m3310, respectively. Mitochondrial RNA gel blot analyses indicated that all four rf1-m alleles in male-sterile plants cosegregated with the altered steady-state accumulation of 1.6 and O.6-kb T-urf13 transcripts, demonstrating that these transcripts are Rf1 dependent. Plants carrying a leaky mutant, rf1-m7323, revealed variable levels of Rf1-associated, T-urf13 transcripts and the degree of pollen fertility. The ability to obtain rf1-m derivatives from Rf1 indicates that Rf1 alleles produce a functional gene product necessary for the accumulation of specific T-urf13 transcripts in T-cytoplasm maize.

Translation Technology

2015 ◽  
Author(s):  
Lynne Bowker ◽  
Gloria Corpas Pastor
Keyword(s):  
Professional Competence ◽  
High Quality ◽  
Translation Tools ◽  
Use Of Technology ◽  
Computer Aided ◽  
Increase Productivity ◽  
Selection Of

In today’s market, the use of technology by translators is no longer a luxury but a necessity if they are to meet rising market demands for the quick delivery of high-quality texts in many languages. This chapter describes a selection of computer-aided translation tools, resources, and applications, most commonly employed by translators to help them increase productivity while maintaining high quality in their work. This chapter also considers some of the ways in which translation technology has influenced the practice and the product of translation, as well as translators’ professional competence and their preferences with regard to tools and resources.

Prescreening in large populations as a tool for identifying elevated CO2-responsive genotypes in plants

2019 ◽  
Vol 46 (1) ◽  
pp. 1 ◽  
Author(s):  
Hiroyuki Shimono ◽  
Graham Farquhar ◽  
Matthew Brookhouse ◽  
Florian A. Busch ◽  
Anthony O'Grady ◽  
...  
Keyword(s):  
Genetic Selection ◽  
Co2 Concentration ◽  
Elevated Atmospheric Co2 ◽  
High Performing ◽  
Active Selection ◽  
Wide Range ◽  
Large Populations ◽  
Increase Productivity ◽  
The Cost ◽  
Selection Of

Elevated atmospheric CO2 concentration (e[CO2]) can stimulate the photosynthesis and productivity of C3 species including food and forest crops. Intraspecific variation in responsiveness to e[CO2] can be exploited to increase productivity under e[CO2]. However, active selection of genotypes to increase productivity under e[CO2] is rarely performed across a wide range of germplasm, because of constraints of space and the cost of CO2 fumigation facilities. If we are to capitalise on recent advances in whole genome sequencing, approaches are required to help overcome these issues of space and cost. Here, we discuss the advantage of applying prescreening as a tool in large genome×e[CO2] experiments, where a surrogate for e[CO2] was used to select cultivars for more detailed analysis under e[CO2] conditions. We discuss why phenotypic prescreening in population-wide screening for e[CO2] responsiveness is necessary, what approaches could be used for prescreening for e[CO2] responsiveness, and how the data can be used to improve genetic selection of high-performing cultivars. We do this within the framework of understanding the strengths and limitations of genotype–phenotype mapping.

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